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Transfer of human membrane surface components by incorporating human cells into intact animal tissue by cell-tissue electrofusion in vivo 总被引:1,自引:0,他引:1
Current animal models employed for the study of the obligate human pathogen Neisseria gonorrhoeae fail to utilize specific human gonococcal attachment receptors required to initiate pathogenesis in a clinically meaningful way. This communication presents evidence that suggests that cell-tissue electrofusion may be employed to create an animal model for this human specific pathogen. This new biotechnology was used to incorporate human membrane gonococcal receptors directly into epithelium of laboratory animals and subsequently infecting the histologically modified tissue with N. gonorrhoeae strain Pgh 3-2. 相似文献
3.
Hartmut Heller Sebastian Brandt Klaus-Dieter Schuster 《Journal of applied physiology》2002,92(4):1770; author reply 1770-1770; author reply 1771
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Human erythrocytes were exposed to oxidative stress by iodate and periodate. Oxidation causes a time- and concentration-dependent increase in membrane permeability for hydrophilic molecules and ions. The induced leak discriminates nonelectrolytes on the basis of molecular size and exhibits a very low activation energy (Ea = 1–4 kcal · mol?1). These results are reconcilable with the formation of aqeous pores. The pore size was approximated to be between 0.45 and 0.6 nm. This increase in permeability is reversible upon treatment with dithioerythritol. Blocking of membrane thiol groups with N-ethylmaleimide protects the membranes against leak formation. The oxidation causes dithioerythritol-reversible modification of membrane proteins as indicated by the gel electrophoretic behavior. These modifications can also be suppressed by blocking the membrane thiol groups with N-ethylmaleimide. About half of the membrane methionine is oxidized to acid hydrolysis-stable derivatives. A fast saturating increase in diene conjugation was observed in whole cells but not in isolated membranes, with only minor degradation of fatty acid chains. The oxidation of cell membrane lipids as well as oxidation of cell surface carbohydrates are not involved in leak formation. Taken together with earlier data (Deuticke, B., Poster, B., Lütkemeier, P., and Haest, C.W.M. (1983) Biochim. Biophys. Acta 731, 196–210), these findings indicate that formation of disulfide bonds by different oxidative mechanisms results in leaks with similar properties. 相似文献
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The urea concentration in the renal cortex ([RC]) of pentobarbital-anaesthetized dogs was 5.71 times higher than the plasma concentration ([P]); the liver ([L]) and the skeletal muscle ([SM]) concentrations were the same as ([P]). Rapid infusion of 20% urea (1 g/kg b.w. within 1 min) was followed by a sudden increase in [P]; [RC] and [L] rose to values nonsignificantly different from [P] and remained non-significantly different for the whole 4 hours of the experiment; at the end, [P] was still about 10 times higher than before infusion. Surprisingly, [SM] 2 and 6 min after infusion was significantly lower than [P]; later they were the same. The experiment thus does not testify to the existence of active transport of urea in the RC. The permeability of the skeletal muscle membrane for urea is lower than that of the RC and liver. Chronic uraemia was induced in rats by transplanting the trigonum vesicae into the peritoneal cavity. In addition to the chemical determination of urea, 14C-urea (marked*) was also measured. [RC]/[P] and [RC*]/[P*] fell as [P] rose; [L], [L*] [SM] and [SM*] never differed from [P] or [P*]. Fluid [PF] collected in the peritoneal cavity had the same chemically determined and radioactive urea concentration as P, but it was hypoosmolar and had a lower [Na+] than P. These experiments likewise did not testify to active urea transport in the RC. It is not clear what caused the osmolarity and sodium gradient between the PF and P, but the peritoneal wall definitely did not act as a simple dialyzing membrane. 相似文献
8.
Sixin Jiang Brigitte Heller Vincent S. Tagliabracci Lanmin Zhai Jose M. Irimia Anna A. DePaoli-Roach Clark D. Wells Alexander V. Skurat Peter J. Roach 《The Journal of biological chemistry》2010,285(45):34960-34971
Stbd1 is a protein of previously unknown function that is most prevalent in liver and muscle, the major sites for storage of the energy reserve glycogen. The protein is predicted to contain a hydrophobic N terminus and a C-terminal CBM20 glycan binding domain. Here, we show that Stbd1 binds to glycogen in vitro and that endogenous Stbd1 locates to perinuclear compartments in cultured mouse FL83B or Rat1 cells. When overexpressed in COSM9 cells, Stbd1 concentrated at enlarged perinuclear structures, co-localized with glycogen, the late endosomal/lysosomal marker LAMP1 and the autophagy protein GABARAPL1. Mutant Stbd1 lacking the N-terminal hydrophobic segment had a diffuse distribution throughout the cell. Point mutations in the CBM20 domain did not change the perinuclear localization of Stbd1, but glycogen was no longer concentrated in this compartment. Stable overexpression of glycogen synthase in Rat1WT4 cells resulted in accumulation of glycogen as massive perinuclear deposits, where a large fraction of the detectable Stbd1 co-localized. Starvation of Rat1WT4 cells for glucose resulted in dissipation of the massive glycogen stores into numerous and much smaller glycogen deposits that retained Stbd1. In vitro, in cells, and in animal models, Stbd1 consistently tracked with glycogen. We conclude that Stbd1 is involved in glycogen metabolism by binding to glycogen and anchoring it to membranes, thereby affecting its cellular localization and its intracellular trafficking to lysosomes. 相似文献
9.
L Bures J Heller K Janácek R Rybová M Kleinová J Kolc P Málek 《Physiologia Bohemoslovaca》1980,29(6):569-575
The authors studied the effect of Mercurascan (MSC) (a hydroxy- mercury derivative of fluorescein) on electrical parameters, namely potential difference (P.D.) and short circuit current (S.C.C.) of frog skin and on the ability of frog bladder tissue to accumulate sodium ions in experiments in vitro. It was found that MSC, in 10(-4) mol/l concentration, reduced the S.C.C., after a brief initial increase, to 5% of the original value and that the P.D. fell steadily right from the outset. In 10(-5) mol/l concentration it raised the S.C.C. by 60% and the increase lasted several hours. The P.D. was unaffected. In 10(-7) and 10(-6) mol/l concentration MSC had no effect on the NA+ content of a nonpolarized frog bladder tissue preparation, but a 10(-5) nol/l concentration sharply reduced it. The effect of MSC on membrane Na+--K+ ATPase, i.e. on the energy metabolism of cellular tissue, is discussed with reference to these results. 相似文献
10.
Oxidative stress of human erythrocytes by iodate and periodate. Reversible formation of aqueous membrane pores due to SH-group oxidation 总被引:2,自引:0,他引:2
Human erythrocytes were exposed to oxidative stress by iodate and periodate. Oxidation causes a time- and concentration-dependent increase in membrane permeability for hydrophilic molecules and ions. The induced leak discriminates nonelectrolytes on the basis of molecular size and exhibits a very low activation energy (Ea = 1-4 kcal.mol-1). These results are reconcilable with the formation of aqueous pores. The pore size was approximated to be between 0.45 and 0.6 nm. This increase in permeability is reversible upon treatment with dithioerythritol. Blocking of membrane thiol groups with N-ethylmaleimide protects the membranes against leak formation. The oxidation causes dithioerythritol-reversible modification of membrane proteins as indicated by the gel electrophoretic behavior. These modifications can also be suppressed by blocking the membrane thiol groups with N-ethylmaleimide. About half of the membrane methionine is oxidized to acid hydrolysis-stable derivatives. A fast saturating increase in diene conjugation was observed in whole cells but not in isolated membranes, with only minor degradation of fatty acid chains. The oxidation of cell membrane lipids as well as oxidation of cell surface carbohydrates are not involved in leak formation. Taken together with earlier data (Deuticke, B., Poser, B., Lütkemeier, P. and Haest, C.W.M. (1983) Biochim. Biophys. Acta 731, 196-210), these findings indicate that formation of disulfide bonds by different oxidative mechanisms results in leaks with similar properties. 相似文献