拉萨地区林周盆地设兴组上部(古近系)年代研究的新进展及意义

拉萨地区林周盆地典中那玛剖面设兴组上部新发现的孢粉组合以落叶、阔叶植物为主体,主要为桦科的Alnipollenites,Betulaepollenites,Carpiniptes,山毛榉科的Quercoidites,胡桃科的Juglanspollenites,榆科的Ulmipollenites,椴科的Tiliapollenites等,孢粉化石多为古近纪常见分子,未发现白垩纪的特征分子。古近纪早期大量出现的三孔沟,网面三孔沟等花粉少量出现;孢粉组合更接近于古近纪中晚期的面貌,其时代可能属于晚始新世。因此,设兴组上部的年代可能是始新世晚期。由此推论,设兴组和林子宗群之间的角度不整合不能代表白垩纪古近纪之间的构造运动,而是代表始新世晚期后的构造运动。同时,林周盆地可能沉积有古近纪的河湖相地层。     

七种灯台报春组植物的细胞学研究

对报春花科（Primulaceae）报春花属（Primula）灯台报春组植物Section Proliferoe的7种报春进行了细胞学研究,其中腾冲灯台报春和川东灯台报春的核型分析为首次报道,结合已报道的灯台报春组其它植物的细胞学资料进行统计分析,结果表明灯台报春组植物在染色体基数、染色体形态、着丝点位置及染色体对称性上都具有很高的一致性,在灯台报春组有核型记录的种类中,其核型都属于Stebbins的2A型或2B型,核型差异很小,染色体基数均为x=11,推测其可能与报春花属中具有相同染色体基数的组亲缘关系更近。结合已发表的灯台报春组植物的细胞学资料及它们的形态特征,对其系统演化关系进行了比较分析,以期对该组的系统学及演化关系提供一些证据。     

浙江煤山剖面二叠纪末的小有孔虫动物群

通过对浙江长兴煤山剖面长兴阶上部和二叠系三叠系界线地层进行系统切片和研究,共鉴别出小有孔虫化石18属50种。这一丰富的有孔虫动物群,不仅进一步完善了长兴阶标准剖面的化石资料,而且也为研究二叠纪末的生物灭绝形式提供了新的依据。     

江西全南小慕泥盆-石炭系孢子组合及其地层意义

据30属57种(包含9新种)小孢子的垂直分布特征,将江西全南小慕泥盆一石炭系的小孢子划分为3个组合(自下而上):1)Acanthotriletes denticulatus-Apiculireiusispora raris(DR)组合,2) Leiotriletes macrothlis-Grandispora xiamuensis(MX)组合,uispinosa(TT)组合,3)Apiculiretusisporatera-Lycospora cf. thuispinosa(TT)组合.这3个组合分别产于三门滩组、翻下组与荒塘组-刘家塘组.据孢子资料的分析与植物、腕足类、鱼类等化石的佐证,三门滩组与翻下组的时代均属晚泥盆世晚期(Famennian),荒塘组与刘家塘组属早石炭世早期(Tournaisian).本区泥盆系与石炭系的界线置于翻下组与荒塘组之间较适合.     

斯勒卷瓣兰，中国云南南部兰科一新种（英文）

描述并绘制了云南南部兰科石豆兰属一新种：斯勒卷瓣兰(Bulbophyllum dresslerianum Z. D. Han & H. Wang)。该新种属于双叶卷瓣兰组(section Tripudianthes Seidenf.),在形态上与该组的4种,即狄氏卷瓣兰(B. dickasonii)、堪布里石豆兰(B. kanburiense)、皱掌卷瓣兰(B. rugosisepalum)和拟双叶卷瓣兰(B. tripudians)近似,主要区别在于该种唇瓣前缘具囊泡,侧面及腹面被稀疏的短腺毛;侧萼片棕黄色,表面光滑。编制了斯勒卷瓣兰及其近缘种的检索表。目前,该种仅在云南南部墨江县发现一个居群,濒危状况有待评估。     

An assay specific for the active form of liver phosphorylase kinase

An assay specific for the active form of liver phosphorylase kinase (EC 2.7.1.38) has been developed utilizing inhibition of the inactive form of phosphorylase kinase by β-glycerophos, phate and ethylene glycol bis(β-aminoethyl ether) N,N′-tetraacetic acid. Following in vitro activation the results compared favorably with those obtained using a less specific assay previously available. (J. R. Vandenheede, S. Keppens, and H. DeWulf, 1977, Biochim. Biophys. Acta.481, 463–470;D. D. Doorneweerd, A. W. H. Tan, and F. Q. Nuttall, 1978, Diabetes27, 474). The in vitro activation of phosphorylase kinase was not associated with the formation of a small-molecular-weight form of the enzyme. The utility of the assay in monitoring in vivo interconversion reactions in response to various physiological stimuli was demonstrated.     

Flora Palinologica Italiana,Sezione Aeropalinologica-Specie esotiche S229:Thuja orientalis L. (Cupressaceae); S230:Cryptomeria japonica (L. fil.) D. Don (Taxodiaceae)

Summary The morphopalynological cards ofThuja orientalis L. and ofCryptomeria japonica (L. fil.) D. Don were illustrated as part of a more comprehensive study on ornamental Pinophyta. Pollens from the original area of the two species were also compared with grains coming from plants cultivated in Italy. In the case ofCryptomeria japonica (L. fil.) D. Don DV, D01, D02, exine, min. and max. diameters, calculated on a japanese sample, differed significatively from the italian sample. In the case ofThuja orientalis L. no significant differences were observed. Pollen from the two italian sites did not show significative differences in size and shape. Pollens acetolysed with the traditional method (Erdtman, 1960) were compared with pollens treated with the Tatzreiter method (1985). Morphometric analysis showed similar values for both species.     

Preparation and properties of fluorescent polysaccharides

A new method for preparing fluorescein derivatives of polysaccharides is described. These derivatives are prepared by activation of the polysaccharide with cyanogen bromide and subsequent reaction with fluoresceinamine. The optimum conditions for coupling have been established in this report. Using this procedure, we have prepared fluorescein derivatives of a wide variety of polysaccharides. Degrees of substitution in the range of 3.0 X 10(-3) to 2.4 X 10(-2) mol of fluorescein per mole of monosaccharide equivalent were obtained. The fluorescent derivatives are stable: no free fluorescein was detected after incubation at 22 degrees C for 48 h or at -10 degrees C for 4 months. The fluorescein-derivatized polysaccharides were found to have the same potency in inhibiting lectin-mediated hemagglutination as the underivatized polysaccharide. In addition, these fluorescent polysaccharides can be radioiodinated to specific activities exceeding 10(6) dpm/micrograms due to incorporation of 125I into fluorescein. The cell binding properties of 125I-fucoidin and 125I-heparin are indistinguishable from the corresponding underivatized polysaccharides. This general approach for preparing fluorescent polysaccharides should produce useful reagents for localizing and quantifying cell surface carbohydrate-binding proteins (lectins).     

Interaction of heparin with antithrombin III and platelet factor 4: pH dependence demonstrated with a new rocket precipitin electrophoretic procedure

Only 30% of commercial heparin reacts with antithrombin III (ATIII). This study shows that the interaction is pH dependent: 100% of the heparin binds to ATIII at pH 3.0, 30% at physiological pH. Binding of ATIII, platelet factor 4, and protamine to heparin was studied using a new rocket precipitin electrophoresis procedure, adapted from the Laurell rocket immunoelectrophoresis procedure. Protamine is incorporated into agarose gel, and heparin mixtures with protamine, ATIII, or platelet factor 4 electrophoresed into the gel from a series of wells. The residual free heparin is precipitated by the protamine in a rocket-shaped arc, the height of which is proportional to the amount of free heparin. No antibody is employed. This procedure is useful for quantitation of heparin and for studying the binding of heparin to proteins.