Neuroplasticity of the spinal cord following electroacupuncture (EA) has been demonstrated although little is known about
the possible underlying mechanism. This study evaluated the effect of EA on expression of neurotrophins in the lamina II of
the spinal cord, in cats subjected to dorsal rhizotomy. Cats received bilateral removal of L1–L5 and L7–S2 dorsal root ganglia
(DRG, L6 DRG spared) and unilateral EA. They were sacrificed 7 days after surgery, and the L6 spinal segment removed and processed
by immunohistochemistry and in situ hybridization histochemistry, to demonstrate the expression of neurotrophins. Significantly
greater numbers of nerve growth factor (NGF) and neurotrophin-3 (NT-3) positive neurons, brain-derived neurotrophic factor
(BDNF) immunoreactive varicosities and NT-3 positive neurons and glial cells were observed in lamina II on the acupunctured
(left) side, compared to the non-acupunctured, contralateral side. Greater number of neurons expressing NGF mRNA was also
observed on the acupunctured side. No signal for mRNA to BDNF and NT-3 was detected. The above findings demonstrate that EA
can increase the expression of endogenous NGF at both the mRNA and protein level, and BDNF and NT-3 at the protein level.
It is postulated that EA may promote the plasticity of the spinal cord by inducing increased expression of neurotrophins. 相似文献
Under conditions of the formalin test, we studied changes in the level of analgesia induced by the action of low-intensity
microwaves on the antinociceptive acupuncture point (AP) E36 in mice of strains CBA/CaLac (CBA) and C57BL/6j (C57) and in
albino mongrel mice. Measurements were performed under control conditions and with experimentally induced decrease in the
serotonin level in the brain (by injections of DL-parachlorophenylalanine, p-CPA). In the latter cases, the duration of the pain behavioral reaction increased despite irradiation of the AP E36. In mongrel,
CBA, and C57 mice, the intensity of pain manifestations was 114.4, 29.0, and 21.1% greater, respectively, than in mice of
these groups with no injections of p-CPA. These facts show that the serotonergic brain system is profoundly involved in the formation of analgesia after irradiation
of the AP by low-intensity microwaves, and this involvement significantly depends on the genotype of the animals.
Neirofiziologiya/Neurophysiology, Vol. 38, Nos. 5/6, pp. 495–497, September–December, 2006. 相似文献
Acupuncture has been an effective treatment for various pain in China for several thousand years. However, the mechanisms underlying this mysterious ancient healing are still largely unknown. Here we applied photoacoustic microscopy (PAM) to investigate brain hemodynamic changes in response to electronic acupuncture (EA) at ST36 (Zusanli). Due to the high optical absorption of blood at 532 nm, PAM could sensitively probe changes in hemoglobin concentration (HbT, i.e., cerebral blood volume [CBV]) of cortical regions in high resolution. Six healthy mice were stimulated at the acupoint and three healthy mice were stimulated at sham points. Remarkable CBV changes in sensorimotor and retrosplenial agranular cortex were observed. Results showed the potential of PAM as a visualization tool to study the acupuncture effect on brain hemodynamics in animal models.
( a ) Schematic showing the stimulation points. ( b ) B‐scan images overlaid with mouse atlas. ( c ) & ( d ) Statistical results of CBV changes from cortical regions. 相似文献