健脾益气解毒方联合化疗治疗晚期结直肠癌的疗效评价

目的:探讨健脾益气解毒方联合化疗治疗晚期结直肠癌的疗效及其对患者免疫功能、生活质量的影响。方法:选择2010年6月至2015年12月在我院进行治疗的晚期结直肠癌患者80例,随机分为两组,每组40例。对照组患者接受FOLFIRI化疗,观察组患者在对照组基础上服用健脾益气解毒汤。比较两组患者治疗的临床疗效、治疗前后机体免疫功能、生活质量的变化情况及治疗期间不良反应的发生情况。结果:治疗后,观察组患者治疗的疾病控制率、CD4~+以及CD4~+/CD8~+、KPS评分均显著高于对照组(P0.05),中医证侯评分及脾虚证候评分、白细胞减少、Ⅰ-Ⅱ级中性粒细胞减少以及肝损伤等不良事件的发生率均显著低于对照组(P0.05)。结论:健脾益气解毒汤联合化疗治疗晚期结直肠癌可有效提高临床疗效,改善患者免疫功能及生活质量。     

柴胡解毒汤对实验性酒精性肝纤维化复合模型大鼠Col-IV，HA的影响

摘要 目的：观察柴胡解毒汤对实验性酒精性肝纤维化复合模型大鼠Col-IV、HA血清含量的影响。方法：通过采用酒、猪油混合灌胃诱导大鼠实验性酒精性肝纤维化复合模型,并分为柴胡解毒汤干预I组（简称干预I组）、柴胡解毒汤兼酒精高脂饮食组（简称干预II组）、酒精兼高脂饮食组（简称模型组）及空白对照组予以灌胃,最终取大鼠血清进行检测。称量大鼠体重,并用ELISA Kit法检测透明质酸(HA)和IV型胶原（Col-IV）的含量。结果：12周后,造模各组大鼠毛发灰暗,杂乱,消瘦,活动度减弱,大便溏稀。血清Col-IV、HA较空白对照组均有显著升高（P＜0. 01）,与模型组比较,干预I组和干预II组均下降明显（P＜0.01）。干预I组、干预II组、模型组、空白对照组HA含量依次为219.17±41.64、184.89±22.83、249.32±34.60、123.48±25.85。Col-IV含量依次为9.59±1.81、12.57±1.41、19.71±6.04、7.62±5.36。结论：柴胡解毒汤可能通过抑制Col-IV、HA的表达水平,对大鼠实验性酒精性肝纤维化复合模型具有一定的干预作用。     

补阳还五汤对中风大鼠脑组织中ROS生成的抑制作用

诱发脑中风的分子机理至今尚未清楚,临床研究表明经典验方补阳还五汤治疗脑中风作用显著,但其作用的分子机理也不清楚。运用低温电子顺磁共振(EPR)技术和自旋捕捉EPR技术实验研究了中风大鼠脑组织中ROS的生成情况;并首次直接测定了补阳还五汤对中风大鼠皮层及小脑的活性氧物质(ROS),包括一氧化氮生成的影响。同时还检测了补阳还五汤对体外化学模拟体系产生的超氧阴离子(O2-)和羟自由基(OH)的清除能力。结果表明,与对照组相比,中风大鼠皮层及小脑中的ROS生成明显增多(P<0.05),脑组织中的NO水平也明显升高(P<0.001);补阳还五汤可明显(P<0.05)抑制中风大鼠皮层及小脑中ROS的生成,显著降低NO水平(P<0.05,与中风不给药组比较);补阳还五汤对于体外化学模拟反应生成的02-和OH也有很强的清除能力。由此可认为ROS的生成是导致脑中风的重要分子机理之一,而抑制ROS的生成则可能是补阳还五汤抗脑中风作用的途径之一,同时也说明在中药现代化研究中EPR技术可作为一种有效的研究工具。     

CD4+CD25+FOXP3+ T cells,Foxp3 gene and protein expression contribute to antiasthmatic effects of San’ao decoction in mice model of asthma

ObjectiveSan’ao decoction (SAD) is a commonly used traditional combinatorial formula composed of Herba Ephedrae, Radix Glycyrrhizae and Amygdalus Communis Vas. Early studies showed that in the OVA sensitization asthmatic mice model its compatibility could lower airway reactivity and airway inflammatory cell infiltration. Based on the above results, this study mainly discussed San’ao decoction's immunomodulatory effects on Tregs.MethodsUPLC–PDA–TOF-MS was applied to analyze chemicals of SAD, and under the optimized chromatographic and MS condition, the major components in SAD were well separated and detected within 22 min. An asthma model was established in BALB/c mice that were sensitized and challenged with ovalbumin. After 2 weeks’ treatment, peripheral blood and bronchoalveolar lavage fluid (BALF) were assessed for inflammatory cell counts; histological change of lung tissue were detected; flow cytometry detection of splenic CD4⁺CD25⁺Foxp3⁺ Treg cells of the mice were counted; Foxp3 expression in lung tissues were examined as well.Results22 Peaks signal chemical components in SAD were identified by UPLC–QTO-MS method. In terms of the percentage of eosinophile in peripheral blood and bronchoalveolar lavage fluid (BALF), SAD groups were significantly lower (p < 0.01) than model group. Compared with model group, lung histological changes of SAD groups were reduced; the proportion of CD4⁺CD25⁺Foxp3⁺ Treg cells in CD4⁺ cells of asthmatic mice also decreased; SAD significantly increased the proportion of CD4⁺CD25⁺Foxp3⁺ Treg cells and promoted Foxp3 expression in a mouse model of asthma.ConclusionThese results suggest that the antiasthmatic effects of SAD are at least partially associated with CD4⁺CD25⁺Foxp3⁺ Treg cells.     

黄芪当归汤联合莫沙比利治疗功能性便秘的疗效及对血清胃肠激素水平的影响

目的:研究黄芪当归汤联合莫沙比利治疗功能性便秘的临床疗效及对血清胃肠激素水平的影响。方法:选择2015年3月至2016年5月我院接受治疗的功能型便秘患者86例。按照随机数字表法分为观察组与对照组,各43例。对照组患者给予莫沙比利治疗,观察组同时加用黄芪当归汤治疗,对比两组临床疗效、不良反应;检测患者治疗前后血清胃动素(MTL)、P物质(SP)水平;采用SF-36生活质量量表对患者治疗后的生活质量进行评价。结果:观察组治疗总有效率为95.35%(41/43),显著高于对照组的81.40%(35/43),差异有统计学意义(P0.05)。两组腹痛、皮疹、稀便发生率对比,均无统计学意义(均P0.05)。治疗前两组血清胃肠激素水平对比无显著差异,治疗后两组MTL均降低,而SP均上升,且治疗后观察组MTL水平显著低于对照组,SP水平显著高于对照组(均P0.05)。治疗后观察组患者饮食、精神、睡眠以及心理四个维度的生活质量评分均显著高于对照组,差异均有统计学意义(均P0.05)。结论:黄芪当归汤联合莫沙比利治疗功能性便秘具有显著的临床疗效,能有效改善患者血清胃肠激素水平,提高患者生活质量,且安全可靠,值得推广应用。     

四磨汤口服液对脾虚便秘小鼠肠道黏膜厚度、隐窝深度和绒毛高度的影响

目的探讨四磨汤口服液对脾虚便秘的治疗机制。方法将实验动物随机分为正常组、脾虚便秘模型组和脾虚便秘治疗组。通过灌胃番泻叶水煎液7d,控制饮食、饥饱失常8d建立小鼠脾虚便秘模型,造模成功后,脾虚便秘治疗组给予四磨汤口服液灌胃,治疗5d,模型组和正常组给予等量无菌水灌胃。结果脾虚便秘治疗组小鼠的空肠肠黏膜厚度明显小于正常组和模型组(P<0.05);模型组小鼠与正常组相比在回肠隐窝深度和肠黏膜厚度上增加极显著(P<0.01),脾虚便秘治疗组小鼠与正常组相比能显著增加回肠绒毛的高度、隐窝深度和肠黏膜厚度(P<0.05或P<0.01);模型组小鼠与正常组相比在盲肠绒毛高度上增加显著(P<0.05),脾虚便秘治疗组小鼠与正常组相比能明显增加盲肠的绒毛高度及其肠黏膜厚度(P<0.01或P<0.05)。结论四磨汤口服液能保护肠黏膜,促进营养物质的吸收。     

麻杏五子汤联合痰热清治疗肺心病急性加重期疗效

目的:探讨麻杏五子汤联合痰热清治疗肺心病急性加重期疗效。方法:回顾性分析150例CPHD急性加重期患者临床资料,根据治疗方法的不同分为A、B、C三组。A组单纯给予西医治疗,B组加用痰热清,C组在西医治疗基础上,给予麻杏五子汤联合痰热清治疗,比较三组患者治疗效果及对Pa CO2、Pa O2、PAMP水平的影响。结果:1治疗2周,A组、B组、C组治疗有效率分别为86%、91.49%、100%,组间比较差异有统计学意义(P0.05)。2C组治疗后咳嗽、咯痰、气喘、肺部啰音评分优于A、B两组,B组优于A组,组间比较差异有统计学意义(P0.05);3治疗后Pa CO2、PAMP下降幅度、Pa O2上升幅度C组B组A组,组间比较差异有统计学意义(P0.05)。结论:麻杏五子汤联合痰热清治疗肺心病急性加重期疗效显著,可有效改善血气指标及肺动脉压。     

小半夏加茯苓汤对小鼠移植心存活时间的延长效果研究

目的:探讨小半夏加茯苓汤对小鼠心脏移植模型中移植心存活时间的延长效果及作用机制。方法:将接受过腹腔心脏移植手术的小鼠分为实验组和对照组,自移植手术当日起至术后7天,每日经口给药,通过腹部触诊判断移植心脏跳动情况,移植术后30日,取部分小鼠移植心脏进行病理检查,取脾细胞进行淋巴细胞培养,并检测培养液中白细胞介素(IL)-2、IL-4、IL-10和干扰素(IFN)-γ的浓度。取移植心脏存活时间超过30天的小鼠脾脏,通过流式细胞术检测调节性T细胞(Regulatory Tcells,Tregs)的含量。结果:与对照组相比,实验组的移植心存活时间明显延长(P0.01),心脏病理切片结果提示实验组的心脏血管病变受到了抑制,脾细胞培养液中IL-2和IFN-γ浓度明显降低,而IL-4和IL-10的浓度升高,脾细胞CD4阳性细胞中CD25和Foxp3均阳性的细胞含量明显高于对照组中同类细胞含量。结论:小半夏加茯苓汤可以显著延长小鼠移植心脏的存活时间,可能与其诱导调节T细胞的产生、改变受体小鼠体内Th-1和Th-2细胞因子的平衡及抑制IL-2、IFN-γ减轻心脏损伤有关。     

四君子汤对实验性脾虚小鼠肠道菌群的影响

本文报道了四君子汤对脾虚小鼠肠道内与人类关系密切的主要菌群的影响。用大量大黄水煎液灌胃给予小鼠,造成实验性脾虚模型,引起小鼠肠道内菌群紊乱,其中双歧杆菌、乳杆菌菌量均下降,与对照组小鼠相比具有显著性差异。当小鼠服用四君子汤后,双歧杆菌,乳杆菌均上升至正常值水平。四君子汤对拟杆菌数量也有一定的影响。本研究表明,四君子汤对小鼠肠道菌群的失调具有调节功能,对脾虚小鼠的康复具有一定的促进作用。     

Antibacterial activity of plant extracts from northwestern Argentina

AIMS: To determine the antibacterial and cytotoxic activities of aqueous and ethanolic extracts of northwestern Argentinian plants used in folk medicine. To compare the mentioned activities with those of five commercial antibiotics. To identify the compounds responsible for the antibacterial activity. METHODS AND RESULTS: Plant extracts were prepared according to traditional uses in northwestern Argentina. Antibacterial activity was assayed by agar dilution in Petri dishes and broth dilution in 96-well plates. Lethal dose 50 (LD(50)) was determined by the Artemia salina assay. Phytochemical analysis was performed by sample adsorption on silica gel, thin-layer chromatography (TLC), bioautography and UV-visible spectra. The results showed that Tripodanthus acutifolius aqueous extracts have lower minimal inhibitory concentrations (MIC) (502 and 506 microg of extracted material (EM) per ml for infusion and decoction, respectively) than cefotaxim MIC (640 microg ml(-1)) against Acinetobacterfreundii (303). These data were lower than their LD(50). Tripodanthus acutifolius tincture showed lower MIC (110 microg of EM per ml) and minimal bactericidal concentration (MBC) (220 microg of EM per ml) than cefotaxim (MIC and MBC of 320 microg ml(-1)) for Pseudomonasaeruginosa. This extract also showed a MIC/MBC of 110/220 microg of EM per ml, lower than oxacillin (MIC/MBC of 160/220 microg ml(-1)) for Staphylococcus aureus (ATCC 25923). The cytotoxicity of all extracts were compared with that of commercial antibiotics. Rutin (3,3',4',5,7-pentahydroxyflavone 3-beta-rhamnosilglucoside), iso-quercitrin (3,3',4',5,7-pentahydroxyflavone 3-beta-glucoside) and a terpene would be partially responsible for the antibacterial activity of T. acutifolius infusion. CONCLUSIONS: Tripodanthus acutifolius extracts had the ability to inhibit bacterial growth. The antibacterial activity differs with the applied extractive method, and it could be partially attributed to glycoflavonoids. This paper contributes to the knowledge of antibacterial capacity of plants from northwestern Argentina. SIGNIFICANCE AND IMPACT OF THE STUDY: These antibacterial activities support further studies to discover new chemical structures that can contribute to alleviate or cure some illnesses.