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51.
灵芝液体发酵条件的优化研究 总被引:2,自引:0,他引:2
通过单因素和正交试验,优化灵芝5760深层培养的营养需求与环境条件对灵芝菌丝体生物量及次生代谢产物发酵液多糖的作用。结果表明,适宜灵芝液体菌种培养和液体发酵的培养基配方为麦麸粉5%,黄豆饼粉3%,KH2PO40.10%,MgSO40.09%;适宜的环境条件为初始pH5.5,装液量32%,生物量最大时为第8~9d;适宜接种的种龄为6d,接种量为10%;发酵周期6d,搅拌转速150r/min,消沫剂添加量可为0.20%。从硒对灵芝5760菌丝体生长及深层发酵生物量的影响,灵芝5760富硒培养适宜浓度为100μg/mL;从加硒时间对生物量的作用,在菌龄适宜时期加入即第1d。 相似文献
52.
Ru-Ming LiuJian-Jiang Zhong 《Phytomedicine》2011,18(5):349-355
In this work, the effects of a pair of positional isomer of ganoderic acids (GAs), namely ganoderic acid Mf (GA-Mf) and ganoderic acid S (GA-S) purified from the fermented mycelia of Ganoderma lucidum, on induction of cell apoptosis and the apoptotic pathway in HeLa cells were investigated. The results demonstrate that both isomers decreased cell population growth on various human carcinoma cell lines by MTT assay, while GA-Mf had better selectivity between normal and cancer cells. The flow cytometry analysis indicated that treatment of HeLa cells with GA-S caused cell cycle arrest in the S phase, while GA-Mf caused cell cycle arrest in the G1 phase. Compared with GA-S, GA-Mf had more potent increase in the number of early and late apoptotic cells. Treatment of HeLa cells with each isomer decreased the mitochondria membrane potential and caused the release of cytochrome c from mitochondria into the cytosol. In addition, stimulation of caspase-3 and caspase-9 activity was observed. The Bax/Bcl-2 ratio was also increased in GA-treated HeLa cells. The results demonstrated that both isomers GA-Mf and GA-S induced apoptosis of human HeLa cells through a mitochondria mediated pathway, but they had the different cell cycle arrest specificity. The findings will be helpful to the development of useful cancer chemopreventive compounds from G. lucidum. 相似文献
53.
一种测定女贞子中齐墩果酸和熊果酸的新方法 总被引:1,自引:0,他引:1
为建立一种加速溶剂萃取-毛细管区带电泳测定女贞子中齐墩果酸和熊果酸的新方法.考察了萃取温度、萃取时间和萃取次数对目标物萃取效率的影响,并考察了硼砂浓度,β-环糊精浓度、pH及甲醇浓度对目标物分离的影响.结果表明:(1)萃取温度和萃取次数影响目标物的萃取效率,而萃取时间的影响很小;萃取压力影响萃取过程的重现性.(2)优化的萃取条件为:萃取压力6.9 MPa,萃取温度100℃,萃取时间5 min和萃取次数2次.(3)优化的缓冲体系为:40 mmol/L硼砂,1 mmol/Lβ-环糊精,pH 9.5及6%甲醇.(4)齐墩果酸和熊果酸分别在10~200、10~160 mg/L范围内线性良好,相关系数均大于0.996,检测限分别为3.2 mg/L和3.0 mg/L,加标回收率为93%~97%.对比了加速溶剂萃取、索氏提取以及超声提取的提取效率.(5)不同提取方法比较结果表明,加速溶剂萃取的提取效率与索氏提取法接近,但高于超声提取;加速溶剂萃取法的主要优点是消耗提取溶剂量少,提取时间短,样品用量小. 相似文献
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Lefki‐Maria Papaspyridi Petros Katapodis Zacharoula Gonou‐Zagou Evangelia Kapsanaki‐Gotsi Paul Christakopoulos 《Engineering in Life Science》2011,11(1):65-74
In this study we maximized biomass production by the basidiomycete Ganoderma australe ATHUM 4345, a species of pharmaceutical interest as it is a valuable source of nutraceuticals, including dietary fibers and glucans. We used the Biolog FF MicroPlate to screen 95 different carbon sources for growth monitoring. The pattern of substrate catabolism forms a substrate assimilation fingerprint, which is useful in selecting components for media optimization of maximum biomass production. Response surface methodology, based on the central composite design was applied to explore the optimum concentrations of carbon and nitrogen sources of culture medium in shake flask cultures. When the improved culture medium was tested in a 20‐L stirred tank bioreactor, using 13.7 g/L glucose and 30.0 g/L yeast extract, high biomass yields (10.1±0.4 g/L) and productivity of 0.09 g L?1 h?1 were obtained. The yield coefficients for total glucan and dietary fibers on biomass formed were 94.82±6 and 341.15±12.3 mg/g mycelium dry weight, respectively. 相似文献
56.
采用单因素试验和响应面分析法优化灵芪菌质多糖固态发酵条件.优化后发酵条件为:采用药性固体发酵培养基,初始pH6.5,接种量10.16ml/100g,发酵温度30.65℃,相对湿度59.54%,发酵周期28d.利用此优化条件进行灵芪菌质多糖发酵,灵芪菌质多糖含量可达9.12±0.09mg/g,较优化前8.06±0.07 ... 相似文献
57.
An insight into spore dispersal of <Emphasis Type="Italic">Ganoderma boninense</Emphasis> on oil palm 总被引:1,自引:0,他引:1
Sanderson FR 《Mycopathologia》2005,159(1):139-141
The disease of oil palm caused by Ganoderma boninense, although universally referred to as Ganoderma basal stem rot, occurs in three very distinct phases, with basal stem rot only part of the disease cycle. G. boninense also causes a seedling disease and an upper stem rot. An understanding of spore dispersal provides an insight into where spores of G. boninense have a role in the infection process. This role will be discussed in relation to each of these three infection phases. This understanding is a critical component of developing a successful disease control strategy. 相似文献
58.
利用深层发酵培养基对两种食药用菌(香菇、猴头菌)进行深层发酵培养,然后分别将香菇(猴头菌)发酵滤液、奶粉、无菌水按不同比例配制成相应的复原奶,并接种乳酸菌制成酸乳。同时检测不同比例发酵滤液添加量所制得的酸乳的营养和理化特性,选择出了最佳比例的滤液添加量,即香菇滤液添加量以3份较好,而猴头菌则以4份滤液添加量最佳。 相似文献
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