HPLC测定灵芝子实体水提物及相关产品中三萜的含量

灵芝药品大多以灵芝子实体水提物为原料,为快速准确测定灵芝子实体水提物及相关产品中三萜的含量,建立了具有较好分离效果的HPLC分析测定方法.通过优化色谱柱和洗脱条件,优选出Agilent Zorbax SB-Aq C18色谱柱(250 mm×4.6 mm,5μm),以乙腈-乙酸水溶液(0.01％)为流动相梯度洗脱,流速为...

Determination of triterpenoids in Ganoderma lingzhi fruiting body water extract and related products by HPLC

Most of the Ganoderma lingzhi medicinal preparations use water extract of fruiting bodies as raw materials. In order to quickly and accurately determine the content of triterpenes in the water extract of G. lingzhi fruiting bodies and related products, a HPLC analysis method with good separation effect is established. Through optimizing the chromatographic column and elution conditions, the Agilent Zorbax SB-Aq C18 chromatographic column (250 mm× 4.6 mm, 5 μm) was selected. Using acetonitrile-glacial acetic acid aqueous solution (0.01%) as mobile phase gradient, under the flow rate of 1.0 mL/min, detection wavelength of 252 nm, and column temperature of 30 °C, 10 ganoderic acids such as ganoderic acid A and ganoderic acid F are well separated. Methodological investigations show that the precision, repeatability, stability, and sample recovery of this analytical method are all less than 5%. This method can be used for quantitative detection of 10 types of ganoderic acids including ganoderic acid C2, ganoderic acid G, ganoderenic acid B, and ganoderic acid B. Comparative analysis of 10 triterpenoids in the raw fruiting bodies, water extracts of fruiting bodies and commercial end products of G. lingzhi indicates that the water extracts of G. lingzhi fruiting bodies contain all of these 10 triterpenes, and the content is 2.52%-6.83% being higher than that in raw fruiting bodies. The triterpene content in the commercial end G. lingzhi products is 0.27%-0.84%. This method advantages the establishment of quality standard of G. lingzhi water extract and its products.