探讨医院图书馆数字化信息建设与服务

数字化信息时代,信息资源建设是主流的业务。本文通过对医院图书馆数字资源建设与知识服务的内容,提出医院图书馆信息资源数字化建设的基本对策和发展方向。     

四阶段四层次医学实验动物学教学模式的建立

通过基础理论、实验操作、应用实践和在职培训四个阶段学习,构建医学特色突出,符合国家法规的医学实验动物学课程体系.针对不同学科专业的培养要求,以及教学对象的素质结构,合理调整教学内容,区别对待研究生、本科生、医学实验专业技术学生和实验动物学专业学员的实验动学教学,制订四层次教学重点与大纲,强化实验动物学与医学生物学的融合,突出专业特点.     

蝴蝶兰LFY基因克隆及高效植物表达载体的构建

根据NCBI中蝴蝶兰LFY花序分生组织基因序列设计2对引物,用RT-PCR法从蝴蝶兰花芽中扩增出LFY基因,对扩增产物进行克隆和测序.结果表明,获得的蝴蝶兰LFY基因约为1500 bp,与报道序列同源性达98.71％.将LFY基因插入pRI101-ON载体中,经PCR、双酶切及测序鉴定,证实重组表达质粒中含有目的片段,表明成功构建了高效植物表达载体pRI1O1-LFY.     

环境微生物学课程内容体系和理论教学模式的探索与实践

环境微生物学是专业基础课,它是开启环境类专业科学大门的一把钥匙,也是破解环境类专业工程难题的一件利器。结合国家精品/资源共享课和学校专业核心课建设,课程组对环境微生物学的课程理论教学模式进行了积极的改革探索,构建了以解答微生物"是什么"、"有何用"和"怎么用"为主线的课程内容体系,提出了以"学生兼课"、"课堂讨论"、"专题讲座"、"课程论文"和"综合评价"为标志的课程理论教学模式。经过实践,成效显著。     

An attempt to dendrochronologically date house features at the King site (9FL5), a 16th century Late Mississippian town in northwestern Georgia,USA

The King site is a Late Mississippian (ca. 1400–1540 CE) aboriginal town located in northwestern Georgia along the Coosa River associated with the Coosa Chiefdom. The site was settled ca. 1530 but was occupied for perhaps only 50 years or so based on the lack of horizontal stratigraphy. The site was visited by members of either or both the Hernando de Soto expedition in 1540 and/or the Tristan de Luna expedition in 1560. In 1974, archaeologists discovered and removed 36 sections of subterranean charred pine posts from six house features. Our objectives were to determine if the tree rings on these posts could be dendrochronologically dated to verify the dates of site occupation and confirm the construction sequence of several houses determined originally via stratigraphic and archaeological evidence. We were able to graphically and statistically crossmatch 13 measurement series representing 10 posts from 5 of the 6 structures, yielding a 157-year floating chronology (average interseries correlation = 0.60). We were unable to absolutely crossdate this floating chronology with the only regional reference chronology long enough (back to 1378 CE) to reach the 16th century, an eastern red cedar chronology from eastern Tennessee. Archaeological evidence indicated Houses 8 and 23.4 were built later in the King site occupancy, confirmed by the tree-ring dates as both houses have the youngest tree rings of the five structures. House 14 had the oldest outermost tree rings but archaeological evidence suggests this house also was likely constructed late in the King site occupancy. We propose some posts were salvaged and reused from abandoned houses as the King site became rapidly depopulated in the last 10–20 years of site occupancy, thus explaining the age of posts used in House 14. We urge archaeologists working in the Southeastern U.S. to consider developing a more formal process for exhuming and preserving charcoalized wood remains from archaeological sites so that these samples can be evaluated using dendrochronological techniques.     

豫北黄河故道湿地岛类自然保护区鸟类区系调查

1997－1999年对豫北黄河故道湿地鸟类自然保护区的鸟类进行了调查,共记录鸟类119种,隶属16目37科,其中留鸟32种,占总数的26．89％,冬候鸟24种,占总数的20．17％,夏候鸟30种,占总数的25．21％,旅鸟占33种,占总数的27．73％,国家一级重点保护鸟类2种,二级重点保护鸟类19种,河南省重点保护的鸟类6种。     

猪线粒体控制区微卫星(mtMs)变异及其分布特征

猪线粒体DNA长度因D-loop中mtMs序列重复数变异而不同。为阐明猪mtMs变异及其在不同群体中的分布特征,本研究对4个藏猪群体、八眉猪、烟台黑猪及3个引入猪群体共164个个体mtDNA D-loop全序进行测定,并与GenBank中发表的相关序列进行比对,分析了其中的重复单元核苷酸变异、重复次数及其在各群体间的分布规律。结果表明,因重复单元“5’-TGCGTACACG-3’”第2~4和10位上碱基变异,猪mtMs形成了以其为核心序列的多种重复单元(R^A^R^G)组成的复合结构,重复数介于3~47之间。单一重复(R^A)组成的mtMs结构在各群体中表现出分布优势,而大多数复合结构(R^AR^B)分布在国外选育群体中。藏猪的mtMs复合结构多达8个,R^AR^CR^E为其特有,另有5个与八眉猪共享。中国野猪和韩国地方猪种也有其特有mtMs结构。丰富的mtMs变异和特有结构与长期适应进化有关,本研究为地方猪种遗传资源及适应性研究提供了标记工具和理论基础。     

KDM3A全长及截短质粒构建及其在细胞中的定位探究

赖氨酸去甲基化酶3A(lysine demethylase 3A,KDM3A)是组蛋白去甲基化酶家族成员之一,通过特异性对组蛋白H3K9甲基化进行去甲基化的作用调控基因转录。目前研究证实KDM3A在肝癌、雌激素受体(estrogen receptorα,ERα)阳性乳腺癌及前列腺癌等肿瘤中发挥促癌作用,但其具体作用机制仍有待更深层次的研究。为深入解析KDM3A在各种肿瘤中的作用及其分子机制,本研究利用分子生物学方法构建了KDM3A基因的全长和截短基因表达质粒,酶切鉴定及测序证实KDM3A全长及截短基因的表达质粒构建成功。在此基础上,再将构建成功的上述多种表达质粒分别转染到HEK293细胞和MCF7细胞中,进行Western blotting和免疫荧光共聚焦实验,进一步对各重组表达质粒在培养的哺乳动物细胞中的蛋白表达及蛋白细胞定位进行初步探究。Western blotting结果显示KDM3A全长基因表达的蛋白约147 kD,其他KDM3A截短基因表达的蛋白均对应其特异性的蛋白条带;免疫荧光共聚焦实验结果显示KDM3A全长质粒以及pN1、pN2、p N3、pΔ截短质粒表达的蛋白分布在细胞核,pC1、pC2截短质粒表达的蛋白分布在细胞质,pC3截短质粒表达的蛋白分别分布于细胞核与细胞质。本实验为后续深入研究KDM3A在肿瘤中的作用及分子机制提供了实验基础。     

A Novel Technique for Efficient Construction of Large scFv Libraries

The development of patient-tailored anti-tumor therapies requires large-scale production of antibodies for the purpose of screening specific tumor antibodies. Thus, the generation of a single chain fragment of variable region (scFv) library with a large repertoire is required for the selection of specific antibodies with high affinity. Presently, the generation of large scFv libraries is impeded by the low efficiency of cloning PCR fragments into phage display vectors, which is due to the low efficiency of the restriction digestion step required in the process. The aim of this study was to increase the efficiency of this critical step. We found methods that are formally believed to facilitate the digestion efficiency, such as adding longer tails to the primers or prolonging the incubation time, were inefficient. We then investigated the feasibility of using T/A cloning to improve the efficiency of cloning and found that when PCR fragments were first cloned into T-vector, and then subsequently subcloned into a phagemid, the cloning efficiency was dramatically increased. Our findings show that by utilizing this method the construction of a large scFv library can be easily accomplished. Aizhi Zhao is the co-first author.     

基因组YAC文库构建方法的几点改进

以pJS97、pJS98为载体, 对中国人基因组YAC文库的构建进行了尝试.建立了一种对小片段DNA“原位电泳筛除”的方案, 采用多胺溶液处理以减少大片段DNA的降解, 使整个建库工作更加简便、有效. 此外, 改进了转化子的挑取和保存的方法, 既简化了操作, 又减少了杂菌污染和交叉污染. 这些改进的方案, 可以推广到其他高等生物基因组YAC文库的构建和应用工作.