利用固定在角叉菜凝胶中的中间埃希氏菌细胞合成L-多巴

将中间埃希氏菌(Escherichia intermedia)细胞用包埋方法固定在角叉菜凝胶中,应用于从邻苯二酚、丙酮酸和氨酶促合成L-多巴。含75mg细胞/克凝胶的制剂保留原酶活性的60～65％。通过对三种底物抑制作用进行观察。看到底物浓度对游离细胞和固定化细胞合成L-多巴初速度的影响几乎相似,在分批反应器中,20小时得到7.8克/升以上的L-多巴(产率是0.39克/升小时)。在初速度条件和分批反应器中,固定在角叉菜凝胶中的细胞合成的L-多巴比固定在聚丙烯酰胺凝胶中的细胞要高。     

黑龙江饶河东安镇侏罗-白垩系界线及Buchia新知

一、前言我国东北地区侏罗、白垩纪地层广泛发育,但绝大部分为陆相地层。1958年,黑龙江省地质局徐衍强等,于虎林县龙爪沟地区首次发现含菊石的海相层料,经王义刚等研究,时代属中侏罗世巴通期(Bathonian)(王义刚,1983,100页)。七十年代后期,张弥曼等报道了松辽盆地吉林前郭、农安等地嫩江组合Sungarichthys longicephalus Takai等半咸水海湾相地层的发现,认为时代属晚白垩世早、中期(张弥曼、刘智成,1977;张弥曼、周家健、刘智成,1977);熊宪政等(1976)于黑龙江穆棱下城子、伊林两地     

家兔中脑导水管周围灰质中注射八肽胆囊收缩素（CCK—8）拮抗吗啡镇痛和...

We have reported that intracerebroventricular (i. c. v.) injection of 1-4 ng of CCK-8 to the rat produced a remarkable antagonistic effect on morphine analgesia. In order to study the species specificity and the site of action, CCK-8 was microinjected into the PAG of the rabbit, and its influence on morphine analgesia and electroacupuncture analgesia was observed. The latency of the escape response (ERL) to radiant heat focused on the snout was measured as an index of the pain threshold. Microinjections were made via cannulae chronically implanted into the PAG. The drug solutions were delivered in a volume of 1 microliter, at a speed of 0.125 microliter/min. The ERL was measured for a period of 60 or 70 minutes at 10 min intervals. 1. CCK-8 administered unilaterally to the PAG of the rabbit at a dose of 3 ng antagonized the analgesia induced by morphine (4 mg/kg, i. v.) by 73% (P less than 0.001), and reduced the analgesic effect of electroacupuncture by 67% (P less than 0.001). These effects were dose-dependent within the range from 1.5 ng to 6.0 ng. The effect of CCK-8 was reversed by CCK receptor blocker proglumide (4 microliters, intra-PAG injection). Unsulfated CCK-8 (CCK-us) had no effect in this regard. These results indicate that in the PAG of the rabbit, exogenously administered CCK-8 was capable of antagonizing opioid analgesia by the activation of CCK receptors. 2. Two groups of rabbits were given with morphine (2 mg/kg, i. v.) and simultaneous injection of CCK-8 antiserum (CCK-AS, 1 microliter) or normal rabbit serum (NRS) into the PAG.(ABSTRACT TRUNCATED AT 250 WORDS)     

薄膜氧电极测定光合作用的HCO3^—浓度问题

氧电极法是一种简单方便的测氧技术,可用以测定光合强度。我们在用氧电极研究植物光合作用时,发现目前采用的HCO_3~-浓度都偏高。本文试讨论用氧电极测定植物叶圆片光合作用时的适宜HCO_3~-浓度问题。目前用氧电极测定光合作用所用的HCO_3~-浓度一般为20mmol/L,亦有用1mmol/L,15mmol/L或100mmol/     

菜粉蝶幼虫S型生长曲线及其拐点的初步研究

菜粉蝶Artogeia rapae(L.)是十字花科蔬菜最重要的害虫之一。国内关于该虫的研究颇多。僵至今尚未见报道其幼虫生长曲线和拐点。本文以衡阳市第一代菜粉蝶幼虫为材料,对其“S”型生长曲线进行了初步研究,并求得其拐点位于第5龄初期,亦即药剂防治的最佳时期。 材料与方法 1.试虫 1986年3月自田间甘蓝上采得第一代菜粉蝶卵,置于室内孵化,幼虫用新鲜甘蓝叶单饲。将同一天孵化的幼虫分成两组,分别作为称重和观察历期试虫。供称重用的幼虫,自孵化之日起,每天均在同一时刻称重1次,直至化蛹,计3批112头。     

用双向Southern转移-杂交法进行克隆的基因分析

用双向Southern转移和分子杂交方法,分析证明了SRSV有关的克隆pSF11插入子含有LTR结构、非编码区、gag基因和部分env基因。并讨论了该方法的优缺点。