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Four factors influence on transformation of indica rice, which were high osmotic treatment; different explant as the target tissue; pressure of rupture disk and quantity of plasmid DNA, were investigated in this experiment. High osmotic treatment of target tissue prior to and after bombardment increased 3.2-fold for Gus transient expression than control. The best treatment of high osmotic was that the target tissues were kept in the target-bed medium which contained 0.4-0.6 mol/L sorbitol and manitol each for 4 h prior to bombardment and for 16 h after bombardment. Four explants: scutellum from mature seed, young panicle, embryogenic callus and suspension cells of indica rice were tested as target explant by particle bombardment. The results of Gus transient showed that the highest expression was scutellum and for other three explants, the order from high to low was young panicle, embryogenic callus and suspension cell. Transgenic plants were obtained from all of the explants except young panicle. For the pressure of rupture disk on transformation, 1100 psi or 1300 psi of the pressure of rupture disk were best one for the transformation and higher than 1300 psi could damage the target tissue which become black and died in the following culture duration. For the quantity of plasmid DNA, the results showed that 0.83 microgram of plasmid DNA per bombardment was preferred for the transformation of indica rice. 相似文献
123.
籼稻和粳稻中蜡质基因座位上微卫星标记的多态性及其与直链淀粉含 … 总被引:10,自引:0,他引:10
稻米直链淀粉是在由蜡质基因Wx编码的颗粒结合淀粉合成酶(GBSS)的催化下合成的,最近,在Wx基因的区段内发现了一段多态性微卫星序列(CT)n,对74个非糯籼稻和粳稻材料的(CT)n多态性进行了分析,并探讨了其与直链淀粉含量之间的关系,在74个品种(系)中共发现7种(CT)n片段(Wx等位基因)邓(CT)8,(CT)10,(CT)11,(CT)16,(CT)17,(CT)18,(CT)19,在籼粳 相似文献
124.
高产优质早籼稻品种粤香占的生长发育特性 总被引:1,自引:0,他引:1
1998年早季在广东省农业科学院水稻研究所内,观察研究了粤香占的部分形态生理特性,结果认为这个常规稻新品种属高收获指数型,收获指数达0.6以上,而且是早季稻早熟种,其从播种到始穗历期为84 d。这个品种的生育前期生长优势较强,具有叶鞘/叶片长度比值小、LAI和干物质增长快、有效分蘖数量多的特点。 相似文献
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采用针对人SRY基因及X与Y染色体同源序列的两对引物进行多重聚合酶链反应技术检测204例新生儿脐血DNA,男性均显示590bp、 355bp及280bp 3条扩增带,女性只有590bp1条扩增带,性别鉴定准确率为100%。又检测47例性器官异常患,13例社会性别为女性者只出现590bp扩增带,与核型性别结果一致。 34例社会性别为男性者中,有2例SRY基因检测结果阴性,只出现590bp带,核型为46,XX,证明这两例患者社会性别不相符,经病理证实后应诊断为女性假两性畸形。
Abstract:This investigation adapted multiplex PCR technique with two pairs of primer to determine sex according to the human SRY gene and X,Y chromosome analogical sequence.We detected bellybutton blood DNA from 204 newborns.Male revealed three bands:590bp,355bp and 280bp,and females only had one band which was 590bp,the accuracy of sex determination was 100%.47 sexual abnormal patients were tested.The results showed that 13 cases,whose social sex were female,had one 590bp band which consistent with their chromosome type sex.In the rest 34 cases,whose social sex were male,2cases showed,on the contrary,only one 590bp band and their chromosome type were 46,XX.This proved that the two patients did not consistent with their social sex,and pathological analysis showed that they were female pseud-hermaphroditism. 相似文献
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影响籼稻DNA限制性片段长度多态性检测的因素分析 总被引:1,自引:0,他引:1
对窄叶青(籼稻)和京系17(粳稻)的RFLP进行了系统分析。结果表明,某种酶多态性检测能力与同时检测到此多态性的其余酶的数目之间存在极显著的正相关(r=0.962**)。由此推论,籼粳稻大部分RFLP可能来自大范围DNA的结构变化而不是碱基取代。cDNA克隆虽然具有高度的保守性,但却具有比基因组克隆更高的多态性检测能力。在实验使用的探针范围内,探针长度和检测到多态性无相关性。由探针检测到的多态性位 相似文献
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