豚鼠卵巢囊淋巴孔的发现及卵巢囊超微结构研究

In order to explore its structure and speciality of species, the ovary bursa of guinea pig was studied by using dissecting microscopy and scanning electron microscopy. In this paper, the lymphatic stomata on both internal and external layer of the ovary bursa was frist reported in guinea pig. The results suggested that the stomata in bursa not only provided a pathway to connect the bursal cavity with the lymphatic vessels of bursa and the peritoneal cavity, but also may be involved in the reproduction and local immunity of the ovary. The stomata may play an important role in physiological function of the ovary. At the same time, the structural differences were identified in ovary bursa between guinea pig and golden hamster.     

毛脚卵巢的组织学观察

对3例雌性毛脚的卵巢结构进行组织形态学研究。采用常规方法制片,H.E染色。结果显示卵巢分叶呈条索状,卵泡发育中没有出现卵泡腔,卵泡细胞始终为单层,卵泡膜随卵泡发育成熟由单层转变为多层,闭锁卵泡在卵泡发育的各个不同时期都有出现。     

妊娠和激素干预对豚鼠卵巢囊淋巴孔的影响

In order to study the influence of pregnancy and hormone on the lymphatic stomata of guinea pig's ovary bursa, the lymphatic stomata of ovary bursa were observed during pregnancy and by using exogenous hormone, including serum gonadotrophin (PMSG) combined with human chorionic gonadotropin (HCG) to promote ovulation and androgen to inhibit the ovary. Then the lymphatic stomata in the inner layer of ovary bursa and their absorption functions were observed with scanning electron microscope (SEM) and trypan blue as a tracer respectively. The lymphatic stomata were also counted by the Elescope computer image processing system. We found that the quantity of tracer absorbed by the lymphatic stomata of ovary bursa in pregnant group was more than that in ovulation-promoted group, and least in the androgen-treated group, which had statistical significance between each couples of them (P < 0.05). The opening area of the lymphatic stomata in pregnant, ovulation-promoted and androgen-treated groups were 189.9 +/- 48.7 micron 2/1000 micron 2, 104.4 +/- 31.2 micron 2/1000 micron 2 and 40.5 +/- 18.7 micron 2/1000 micron 2 respectively, which had remarkable statistical difference between each couples (P < 0.01). Under SEM observation, the secretory granules in ciliated columnar epithelium were less in pregnant group than the other two, while both the secretory granules and the cilium were most active in ovulation-promoted group. The results indicated that both the openings and absorption functions of the lymphatic stomata in guinea pig's ovary bursa could be affected by pregnancy and exogenous homone. There existed relationship between ovary function and the regulation of the lymphatic stomata in ovary bursa.     

中华绒螯蟹卵巢差减cDNA文库的构建

应用抑制性差减杂交技术构建中华绒螯蟹卵巢两个发育时期的差减cDNA文库。正向差减杂交以Ⅲ期卵巢为试验方、Ⅱ期卵巢为驱动方,反向差减杂交以Ⅱ期卵巢为试验方、Ⅲ期卵巢为驱动方;将所获差减cDNA片段克隆入质粒表达载体,转化大肠杆菌JM109。最后获得的正、反向差减文库分别含863、360个重组子。PCR扩增鉴定正、反向差减cDNA文库的插入片段平均大小分别为360和160bp,表明所构建的差减言语库适合进一步研究中华绒螯蟹卵巢发育相关基因。     

滋补肾阴方与温补肾阳方对卵巢切除所致骨质疏松大鼠治疗作用的对比研究

目的　对比观察滋补肾阴方与温补肾阳方对卵巢切除所致骨质疏松大鼠的治疗作用 ,以探讨它们之间的疗效是否存在差异。方法　将雌性Wistar大鼠 4 9只随机分为正常对照组、假手术组、卵巢切除组、滋阴组 (灌服生药含量为 1 0 5g ml的滋补肾阴方 ,按每 10 0g体重 0 7ml剂量 )、补阳组 (灌服生药含量为 0 78g ml的温补肾阳方 ,按每 10 0g体重 0 7ml剂量 )。于造模 3个月后开始给药 ,1次 日 ,连续 6d ,休息一天后 ,再连续灌服 6d。给药 2个月后 ,将大鼠处死 ,取胫骨行不脱钙骨切片 ,甲苯胺蓝染色 ,对其进行骨组织形态计量。结果　切除卵巢 5个月后 ,大鼠胫骨TBV %显著降低 ,TRS %以及TFS %、AFS %、MAR、BFR、OSW和mAR皆显著增高 ,给大鼠灌服滋阴补肾方和温补肾阳方 2个月后 ,均能使上述指标发生逆转 ,但程度有所不同 ,温补肾阳方的效果要明显优于滋补肾阴方。结论　滋补肾阴方与温补肾阳方对卵巢切除所致的骨质疏松均有明显的治疗作用 ,但温补肾阳方的疗效要优于滋阴补肾方。     

62例卵巢冠囊肿B超误诊分析

卵巢冠囊肿是来源于阔韧带的病变,在临床B超检查中常出现误诊,错误的诊断为卵巢、输卵管或盒腹其它脏器来源的病变。本文对我院近年经手术证实的62例卵巢冠囊肿患者术前所做的B超检查结果进行了分析,总结出一些误诊的原因和经验教训。1 资料和方法1.1 一般资料 本组患者62例,均是我院1997年～2000年在我院住院的患者,年龄19～67岁,平均45岁。来院就诊主诉各不相同,其中腹胀48例,下腹隐痛20例,不孕12例,要求中止早期妊娠者6     

凹唇壁蜂的耐寒性及生殖潜力研究

在滞育期和滞育结束后 (成虫出茧之前 ) ,分别测定凹唇壁蜂越冬成虫的耐寒性 ,结果表明 :滞育期成虫的过冷却点和体液冰点分别为 -2 6 4 8℃和 -2 0 91℃ ,解除滞育后分别为 -1 8 4 0℃和-1 1 78℃。自然条件下 ,凹唇壁蜂在我国北方大部分地区可安全越冬。在筑巢前期 ,对雌蜂的生殖系统解剖观察发现 ,卵巢由 6根卵巢管组成 ,卵母细胞总数平均为 55 9± 4 4 6个 ,卵原区在成虫筑巢期不再产生卵母细胞。     

抗人P185~(erbB2)嵌合抗体在CHO细胞中的高效表达及其活性分析

为降低人抗鼠抗体 (HAMA)反应并在CHO细胞中高效表达抗人P185 erbB2 人 /鼠嵌合抗体 ,将抗人P185 erbB2 单抗C2 5的轻、重链可变区基因分别克隆入具有人抗体恒定区基因组序列和弱化启动子驱动的选择标志基因的真核表达载体中 ,共转染CHO dhfr-细胞 ,经G418及氨甲喋呤 (MTX)梯度加压筛选进行了嵌合抗体的高效表达。采用RT PCR、ELISA、细胞ELISA、免疫荧光细胞化学等实验证实了所表达的抗人P185 erbB2 嵌合抗体的人源性及抗原特异性。培养上清中的抗体产量可达 10 0mg/L ,所表达的嵌合抗体具有抑制P185 erbB2 高表达肿瘤细胞增殖的作用     

豚鼠卵巢囊淋巴孔的发现及卵巢囊超微结构研究

本实验通过扫描电镜等方法研究豚鼠卵巢囊及卵巢囊淋巴孔的结构,并探讨了卵巢囊及其淋巴孔的种属差异.实验结果首次报道豚鼠卵巢囊内、外层上皮均存在淋巴孔;豚鼠卵巢囊结构在输卵管走行、囊闭合程度及囊表面超微结构等方面与金仓鼠存在差异.结果提示豚鼠卵巢囊内、外层淋巴孔是沟通卵巢囊腔、卵巢囊淋巴系及腹膜腔的形态基础,可能是三者间物质转运的重要途径.卵巢囊淋巴孔可能影响物种的繁殖并参与囊腔内局部免疫反应.卵巢囊结构和发育程度与对应的输卵管伞端等结构及其他生殖特点相适应,研究结果丰富了生殖形态学和比较解剖学资料.     

不同证型多囊卵巢综合征患者的临床和生化特征研究

目的:探讨"痰湿证"和"非痰湿证"多囊卵巢综合征(Polycystic ovary syndrome,PCOS)患者的临床和生化特征。方法:纳入PCOS患者89例(其中痰湿证42例、非痰湿证47例)及正常对照52例(其中痰湿证对照组21例、非痰湿证对照组31例),采集和比较其临床资料及血清糖脂代谢指标、性激素水平的差异。结果:PCOS患者月经初潮年龄明显晚于正常对照组,两种证型PCOS患者均存在临床表现、性激素、糖代谢、脂代谢的异常,表现在多毛评分、黑棘皮、皮肤溢脂、痤疮发生率明显高于对照组;黄体生成素((Luteinizing hormone,LH)、黄体生成素/促卵泡激素(Follicle stimulating hormone,FSH)、雌二醇(Estradiol,E2)、睾酮(Testosterone,T)、游离雄激素指数(Free androgen index,FAI)、空腹葡萄糖(Fast blood glucose,FBG)、甘油三酯(Triglyceride,TG)水平明显高于对照组;高密度脂蛋白(High density lipoprotein,HDL)水平显著低于对照组(P0.05)。而"痰湿型"PCOS以体重指数(Body mass index,BMI)、腰臀比(Waist hip ratio,WHR)、收缩压、黑棘皮和皮肤溢脂发生率、FAI、FBG、空腹胰岛素(Fast Insuline,FINS)、稳态模型胰岛素抵抗指数(Homeostasis model insulin resistance index,HOMA-IR)水平增高为主;而"非痰湿型"PCOS以LH/FSH、LH水平增高为主;与"非痰湿"对照组相比,"痰湿"对照组BMI、WHR、收缩压、FBG、TG、低密度脂蛋白(Low density lipoprotein,LDL)、HOMA-IR明显升高。结论:"痰湿证"PCOS患者以糖脂代谢异常和胰岛素抵抗为主,而"非痰湿证"PCOS患者以性腺轴紊乱和高雄激素血症为主,将PCOS患者分为"痰湿证"和"非痰湿证",能反映疾病不同证型的基本特点,对远期并发症的防治有指导意义。