全文获取类型
收费全文 | 39743篇 |
免费 | 3610篇 |
国内免费 | 4313篇 |
出版年
2024年 | 76篇 |
2023年 | 542篇 |
2022年 | 937篇 |
2021年 | 2075篇 |
2020年 | 1439篇 |
2019年 | 1780篇 |
2018年 | 1636篇 |
2017年 | 1203篇 |
2016年 | 1670篇 |
2015年 | 2561篇 |
2014年 | 2984篇 |
2013年 | 3119篇 |
2012年 | 3838篇 |
2011年 | 3350篇 |
2010年 | 2231篇 |
2009年 | 1883篇 |
2008年 | 2355篇 |
2007年 | 2014篇 |
2006年 | 1821篇 |
2005年 | 1519篇 |
2004年 | 1289篇 |
2003年 | 1149篇 |
2002年 | 1005篇 |
2001年 | 703篇 |
2000年 | 566篇 |
1999年 | 596篇 |
1998年 | 394篇 |
1997年 | 301篇 |
1996年 | 327篇 |
1995年 | 259篇 |
1994年 | 325篇 |
1993年 | 188篇 |
1992年 | 260篇 |
1991年 | 208篇 |
1990年 | 194篇 |
1989年 | 129篇 |
1988年 | 91篇 |
1987年 | 98篇 |
1986年 | 58篇 |
1985年 | 74篇 |
1984年 | 56篇 |
1983年 | 49篇 |
1982年 | 43篇 |
1981年 | 31篇 |
1980年 | 17篇 |
1979年 | 30篇 |
1977年 | 16篇 |
1976年 | 19篇 |
1975年 | 16篇 |
1973年 | 20篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
91.
A solvent mixture containing dioxane, acetonitrile, and hexane was found to be suitable as a mobile phase to resolve oxazepam enantiomers by chiral stationary phase high performance liquid chromatography using covalent Pirkle columns. The resolved oxazepam enantiomers in this solvent mixture had a racemization half-life greater than 3 days at 23°C. When desiccated at 0°C as dried residue, OX enantiomers were stable for at least 50 days with less than 2% racemization. The conditions which stabilized OX enantiomers significantly facilitated the determination of racemization half-lives of OX enantiomers in a variety of aqueous and nonaqueous solvents and at different temperatures. © 1992 Wiley-Liss, Inc. 相似文献
92.
Carbon Assimilation and Leaf Water Status in Sugar Beet Leaves during a Simulated Natural Light Regimen 下载免费PDF全文
Carbon assimilation and leaf water status were studied in sugar beet (Beta vulgaris L., Klein E-type multigerm) leaves during a light period in which illumination either increased rapidly to full irradiance or changed gradually in a sinusoidal manner as generally occurs during a natural day. A light regimen that simulated the light of a natural day was produced by adjusting irradiance with a neutral-density filter under the control of a computer. Under this light regimen, photosynthesis, transpiration, and stomatal conductance followed the irradiance pattern very closely and ribulose bisphosphate carboxylase was nearly fully activated. When illumination was increased rapidly at the beginning of a light period, transpiration also increased quickly, causing leaves to wilt to some extent. The activation state of ribulose bisphosphate carboxylase increased to only 52%, but ribulose bisphosphate level was nearly twice as high as during the simulated natural day. In spite of the differences in activation state and ribulose bisphosphate levels, photosynthesis rates were very similar under both regimens. Nevertheless, differences in parameters between leaves under the two irradiance regimens can affect how a plant responds to internal or external factors, and therefore, the rate at which irradiance increases at the beginning of a light period is an important consideration when interpreting data. 相似文献
93.
B. R. Lu R. von Bothmer 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1991,81(4):524-532
Summary Integeneric hybridizations were carried out between Secale cereale L. (2n = 14, RR) and three Elymus species, namely, E. caninus (L.) L. (2n = 28, SSHH), E. brevipes (Keng) Löve (2n = 28, SSYY) and E. tsukushiensis Honda (2n = 42, SSHHYY). Chromosome pairing was studied at metaphase I in the parental species and the hybrids. Meiotic configurations of the hybrids were 20.74 1+0.14 II for E. caninus x S. cereale (SHR), 16.35 I+2.17 II+0.09 III for E. brevipes x S. cereale (SYR) and 25.84 I+1.10 II+0.02 III for E. tsukushiensis x S. cereale (SHYR), in addition to some secondary associations in the different hybrids. It is concluded from the study that (1) a certain, different homoeologous relationship exists among S, H and Y genomes in the investigated Elymus species; (2) low homoeology is present between genomes of Elymus (S or H or Y) and rye (R); (3) the Secale genome affects homoeologous chromosome pairing between different genomes in E. brevipes and E. tsukushiensis. 相似文献
94.
Human chorionic gonadotropin (hCG) is a glycoprotein hormone composed of two dissimilar subunits, alpha and beta. Nicks or missing peptide linkages have been found in the beta 44-52 region of the beta-subunit of hCG, whether from pregnancy or trophoblast disease. This article reviews recent reports about the location of nicks in hCG, their origin and occurrence, their effects on the steroidogenic and receptor-binding activities of hCG, and on the immunological activities of hCG and its free beta-subunit. Taken together, the reports show: (1) nicks occur primarily between beta 47 and beta 48, and to a lesser extent between beta 44 and beta 45; (2) the extent of nicking in hCG samples varies widely, from undetectable to 100 percent of molecules; (3) nicks greatly reduce the steroidogenic activity of hCG in vitro (nicked molecules have less than 20 percent of the activity of the intact hormone); (4) nicks may occur at the trophoblast-myometrial interface or in the circulation by the action of human leucocyte elastase or similar leucocytic protease; (5) hCG testing kits using dimer-specific antibodies may not detect nicked molecules and may give different results from those using other antibodies; (6) hCG international reference preparations and the CR series of hCG standards are variably nicked (10 percent to 20 percent), complicating the problem of discordant hCG results in nick-sensitive assays; (7) results from commonly used immunoassays for measurement of the hCG free beta-subunit vary by as much as tenfold because some of the antibodies employed do not detect nick free beta-subunit. 相似文献
95.
Bao-Rong Lu 《Nordic Journal of Botany》1991,11(1):27-32
Intergeneric crosses have been made between Psathyrostachys huashanica (2x= 14, NN) and three Elymus species, namely, E. pendulinus (2n = 4x = 28, SSYY), E. ciliaris (2n = 4x = 28, SSYY), and E. tsukushiensis (2n = 6x = 42, SSHHYY). Three accessions of E. tsukushiensis from different localities crossed with P. huashanica produced adult hybrid plants. Although completely sterile, the hybrid plants developed rather vigorously, and were morphologically intermediate between the two parents. Chromosome configuration of 24.62I + 1.51II + 0.03III per cell characterized meioses of the hybrids, but there existed slight variations among different combinations. These results indicate that there is little or no chromosome homoeology between "N" genome of P. huashanica and "S", "H" or "Y" genomes of E. tsukushiensis. 相似文献
96.
灭幼脲Ⅲ号在好气水环境中的降解代谢的初步研究 总被引:1,自引:1,他引:0
本文研究了新农药灭幼脲Ⅲ号在好气水环境中的降解与代谢。在避光条件下,观察了灭菌组与实验组中灭幼脲Ⅲ号及其主要代谢产物的消长过程,比较了它的化学水解与微生物降解的差异。在室内模拟好气系统中,研究了母体化合物在水体中的残留动态和生物降解半衰期,及其初期主要代谢途径的转化产物,同时分别用高效液相色谱法,紫外吸收光谱扫描,以及特征有机质谱图,对灭幼脲Ⅲ号的两种主要代谢产物进行了定性定量测定。结果表明:灭幼脲Ⅲ号在室内好气环境中较易水解,而且水中微生物的存在能加速它的降解,母体化合物在水体中初期代谢主要途径为分子中的苯甲酰碳与脲氮键首先开裂,生成邻氯苯甲酸(CBA)和对氯苯基脲素(CPU)。 相似文献
97.
蓝藻型富营养湖泊藻量的昼夜变化节律 总被引:3,自引:0,他引:3
杭州西湖为蓝藻型富营养湖泊,根据1980年9月及11月二次昼夜分层采样调查,该湖浮游藻在一昼夜中有二次上下垂直移位,使近表层藻量形成规则或不规则的双峰型昼夜变化曲线,双峰分别出现在日照开始与日落后2h左右,认为昼夜光暗交替与其昼夜变化有一定相关关系,同时,双峰的形成与蓝藻门优势藻种对光照强度的适应性能相关。文中对其优势藻种的浮沉与日照关系作了讨论。 相似文献
98.
袁晓;卢大炎;王国亮;叶婉成 《武汉植物学研究》1992,10(4):359-362
从卫矛科南蛇藤属植物毛枝南蛇藤(Celastrus hookeri Prain)根皮中首次分离出一个红色立方体形结晶,经紫外、红外、高分辨质谱和~(13)C核磁共振谱分析,并与有关文献数据对照,确认是南蛇藤素。作者用薄层扫描和高效液相两种方法初步测定了毛枝南蛇藤根皮和根的木栓化表皮的南蛇藤素含量,结果表明南蛇藤素主要集中在根的木栓化表皮,含量在12%以上,而在整个根皮中的含量大约为1%。 相似文献
99.
人肌肌酸激酶胍变性时的失活与构象变化的比较研究 总被引:1,自引:1,他引:0
应用二阶导数光谱、紫外差吸收光谱和荧光光谱等监测手段,研究了人肌肌酸激酶在盐酸胍溶液中的构象变化。二阶导数光谱结果表明,若以6M盐酸胍中肌酸激酶酪氨酸残基的暴露程度为100%,则天然酶酪氨酸残基的暴露程度只有2%。而紫外差吸收光谱和荧光光谱的变化与兔肌肌酸激酶的结果相似。比较不同胍浓度下人肌肌酸激酶的失活与构象变化,表明酶的失活先于构象变化。同时还测定了不同浓度胍溶液中人肌酶的失活与构象变化的速度常数。结果表明以几种方法测定的构象变化均为单相的一级过程,而酶的失活却呈现了由快慢两相组成的一级反应过程。比较同浓度胍溶液中的失活速度与构象变化速度,发现酶失活的快相反应速度常数比构象变化的速度常数大1—2个数量级,慢相速度常数与构象变化速度常数相近。上述结果进一步支持了酶的活性部位构象柔性的观点。 相似文献
100.
本实验主要观察并比较了大鼠冷适应前后直肠温度(RT)、血清游离脂肪酸(SFFA)浓度、肩胛间棕色脂肪组织(IBAT)和肝脏cAMP含量的变化及其对去甲肾上腺素(NE)反应性的改变。结果表明:①冷适应28d大鼠在冷环境中RT稳定,NE刺激后RT上升幅度大于常温对照组(P<0.005);②冷适应1d组SFFA升高,冷适应28d组SFFA接近对照组,且对NE刺激无反应,对照组给NE后SFFA与RT一致性升高;③冷适应28d组IBAT的cAMP升高,而肝脏的cAMP含量三组间无显著性差异。NE刺激后,冷适应28d组IBAT和肝脏cAMP均升高,与RT反应一致,而对照组不变。结果提示,在5±3℃适应28d的大鼠已建立冷适应机制,非寒颤产热(NST)容量增加,在冷适应的不同时期,肝脏和IBAT调节NST的机制不同。 相似文献