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991.
Statistics in Biosciences - Weighted log-rank statistics and recently weighted win loss statistics are often used to test the null hypothesis that the treatment group and the control group have no... 相似文献
992.
Hui Xiong Xuefeng Liu Yuhang Fan Shaorong Luan Qingchun Huang 《Journal of Phytopathology》2020,168(10):601-608
Botrytis cinerea, the causal agent of the grey mould disease, developed resistance to multiple fungicides. However, the role of cell membrane in survival competition of B. cinerea upon quinone outside inhibitor (QoI) fungicide has not yet been elucidated. In this paper, the enhancement of cystamine, a transglutaminase inhibitor, on membrane integrity of B. cinerea was determined, and the effect of the enhancement on the sensitivity of B. cinerea to pyraclostrobin was investigated. The results showed that pyraclostrobin inhibited mycelial growth with EC50 as 1.122 and 3.042 μg/ml at 24 and 48 hr, respectively. In the treatment of 5 and 50 μg/ml pyraclostrobin, membrane integrity of B. cinerea was broken, causing high permeability, lipid peroxidation, flocculent and malformed surface with vague septum and abundant agglomerates inside and outside the mycelia. Cystamine even at 50 and 200 μg/ml had little inhibitory effect on mycelial growth. However, in presence of 50 or 200 μg/ml cystamine, the mycelia from pyraclostrobin treatment possessed a significantly reduced leakage, lower MDA content, and a revived fibrous and transparent surface. Meanwhile, SEM images showed that membrane integrity of the mycelia was significantly improved and the agglomerates were dramatically disappeared. Synergy assays further revealed that B. cinerea regained less sensitivity to pyraclostrobin inhibition. In conclusion, membrane integrity controls mycelia sensitivity and is required for survival competition of B. cinerea upon pyraclostrobin. 相似文献
993.
Jun Chen Bo Wang Yan Zheng Hui Jiang Tian Jiang Hakan Bozdoğan Junqiang Zhang Baizheng An Xiaoli Wang Haichun Zhang 《Journal of Zoological Systematics and Evolutionary Research》2020,58(1):174-193
The Mesozoic family Procercopidae is widely treated as the ancient group of Cercopoidea and a transitional unit to recent lineages, but its evolution and diversity are vague due to fragmentary fossil record and confusing taxonomic history. Herein, an extensive taxonomic review of Procercopidae is presented and some new fossils are reported from the Lower Cretaceous Yixian Formation of NE China. As a result, Chengdecercopis Hong, 1983 is transferred from Procercopidae to Sinoalidae; Procercopis longipennis Becker-Migdisova, 1962 and P shawanensis Zhang, Wang and Zhang, 2003 are transferred to Procercopina Martynov, 1937, resulting in Procercopina longipennis (Becker-Migdisova, 1962), comb. n. and P shawanensis (Zhang, Wang and Zhang, 2003), comb. n.; Luanpingia senjituensis Hong, 1984 is transferred to Stellularis Chen, Yao and Ren, 2015, leading to Stellulari senjituensis (Hong, 1984), comb. n.; Anthoscytina macula Hu, Yao and Ren, 2014 is transferred to Sinocercopis Hong, 1982, and Sunoscytinopteris (Scytinopteridae) and Cathaycixius (Cixiidae) are treated as junior homonym names of Sinocercopis, leading to Sinocercopis macula (Hu, Yao and Ren, 2014), comb. n., S lushangfenensis (Hong, 1984), comb. n., S pustulosis (Ren, 1995), comb. n., and S trinervis (Ren, 1995), comb. n. Additionally, two new species are erected: Stellularis bineuris Chen and Wang, sp. n. and S minutus Chen and Wang, sp. n. Our cladistic analysis based on wing (tegmen and hind wing) characteristics recovers the high-level relationships within Cercopoidea: Sinoalidae + (Procercopidae + (Cercopionidae + modern cercopoids)). Within the family Procercopidae, the cladistic analysis reveals that the Middle to Late Jurassic Titanocercopis and Jurocercopis and the Cretaceous Cretocercopis occupy the basal position, and a gradual change in wing venation can be recognized from the Early Jurassic Procercopis and Procercopina to the Jurassic Anthoscytina, and then to the Cretaceous Stellularis and Sinocercopis. The two Cretaceous genera, sharing wing traits with extant cercopoids, likely represent transitional forms between Procercopidae and recent Cercopoidea; however, they are very similar to their Jurassic relatives in body structures, suggesting it is applicable to attribute them to Procercopidae. Furthermore, our analysis suggests that the extinction of Procercopidae and the origin and early diversification of modern Cercopoidea approximately coincided with the rise and explosive radiation of angiosperms in the late Early Cretaceous and onwards. 相似文献
994.
Hui Fang Xiuyi Fu Yuebin Wang Jing Xu Haiying Feng Weiya Li Jieting Xu Orawan Jittham Xuan Zhang Lili Zhang Ning Yang Gen Xu Min Wang Xiaowei Li Jiansheng Li Jianbing Yan Xiaohong Yang 《The Plant journal : for cell and molecular biology》2020,101(2):278-292
The nutritional traits of maize kernels are important for human and animal nutrition, and these traits have undergone selection to meet the diverse nutritional needs of humans. However, our knowledge of the genetic basis of selecting for kernel nutritional traits is limited. Here, we identified both single and epistatic quantitative trait loci (QTLs) that contributed to the differences of oil and carotenoid traits between maize and teosinte. Over half of teosinte alleles of single QTLs increased the values of the detected oil and carotenoid traits. Based on the pleiotropism or linkage information of the identified single QTLs, we constructed a trait–locus network to help clarify the genetic basis of correlations among oil and carotenoid traits. Furthermore, the selection features and evolutionary trajectories of the genes or loci underlying variations in oil and carotenoid traits revealed that these nutritional traits produced diverse selection events during maize domestication and improvement. To illustrate more, a mutator distance–relative transposable element (TE) in intron 1 of DXS2, which encoded a rate‐limiting enzyme in the methylerythritol phosphate pathway, was identified to increase carotenoid biosynthesis by enhancing DXS2 expression. This TE occurs in the grass teosinte, and has been found to have undergone selection during maize domestication and improvement, and is almost fixed in yellow maize. Our findings not only provide important insights into evolutionary changes in nutritional traits, but also highlight the feasibility of reintroducing back into commercial agricultural germplasm those nutritionally important genes hidden in wild relatives. 相似文献
995.
Hui Li Min Wang Weijun Li Linlin He Yuanyuan Zhou Jiantang Zhu Ronghui Che Marilyn L. Warburton Xiaohong Yang Jianbing Yan 《The Plant journal : for cell and molecular biology》2020,103(3):1089-1102
Traditional genetic studies focus on identifying genetic variants associated with the mean difference in a quantitative trait. Because genetic variants also influence phenotypic variation via heterogeneity, we conducted a variance‐heterogeneity genome‐wide association study to examine the contribution of variance heterogeneity to oil‐related quantitative traits. We identified 79 unique variance‐controlling single nucleotide polymorphisms (vSNPs) from the sequences of 77 candidate variance‐heterogeneity genes for 21 oil‐related traits using the Levene test (P < 1.0 × 10?5). About 30% of the candidate genes encode enzymes that work in lipid metabolic pathways, most of which define clear expression variance quantitative trait loci. Of the vSNPs specifically associated with the genetic variance heterogeneity of oil concentration, 89% can be explained by additional linked mean‐effects genetic variants. Furthermore, we demonstrated that gene × gene interactions play important roles in the formation of variance heterogeneity for fatty acid compositional traits. The interaction pattern was validated for one gene pair (GRMZM2G035341 and GRMZM2G152328) using yeast two‐hybrid and bimolecular fluorescent complementation analyses. Our findings have implications for uncovering the genetic basis of hidden additive genetic effects and epistatic interaction effects, and we indicate opportunities to stabilize efficient breeding and selection of high‐oil maize (Zea mays L.). 相似文献
996.
Wenfei Ding Hui Yin Tan Jia Xiang Zhang Luke A. Wilczek Karin R. Hsieh Jeffrey A. Mulkin Piero R. Bianco 《Protein science : a publication of the Protein Society》2020,29(5):1211-1227
The Escherichia coli single‐strand DNA binding protein (SSB) is essential to viability where it functions to regulate SSB interactome function. Here it binds to single‐stranded DNA and to target proteins that comprise the interactome. The region of SSB that links these two essential protein functions is the intrinsically disordered linker. Key to linker function is the presence of three, conserved PXXP motifs that mediate binding to oligosaccharide‐oligonucleotide binding folds (OB‐fold) present in SSB and its interactome partners. Not surprisingly, partner OB‐fold deletions eliminate SSB binding. Furthermore, single point mutations in either the PXXP motifs or, in the RecG OB‐fold, obliterate SSB binding. The data also demonstrate that, and in contrast to the view currently held in the field, the C‐terminal acidic tip of SSB is not required for interactome partner binding. Instead, we propose the tip has two roles. First, and consistent with the proposal of Dixon, to regulate the structure of the C‐terminal domain in a biologically active conformation that prevents linkers from binding to SSB OB‐folds until this interaction is required. Second, as a secondary binding domain. Finally, as OB‐folds are present in SSB and many of its partners, we present the SSB interactome as the first family of OB‐fold genome guardians identified in prokaryotes. 相似文献
997.
本研究将microRNA插入EF1α启动子的内含子中,构建携带沉默PD-1基因的miRNA的新型慢病毒载体,并将其应用于CAR-T细胞。通过流式细胞术检测慢病毒载体转导效率和PD-1沉默效率;Westernblotting检测PD-1蛋白表达差异;荧光定量PCR检测microRNA相对表达情况;荧光素酶生物发光法和流式细胞术检测CAR-T细胞的能力。结果显示与U6转录microRNA的载体相比较,将microRNA插入到EF1-α内含子中的病毒载体转导效率更显著,对PD-1的敲低效率均达90%以上,且Westernblotting结果验证了PD-1的敲低效果。另外通过荧光定量PCR,可显示出转导该新型慢病毒载体的Jurkat细胞内microRNA的相对表达量。荧光素酶生物发光法证实了CAR-T细胞针对靶细胞的特异杀伤性,流式细胞术结果表明沉默PD-1的CAR-T细胞相较于正常CAR-T细胞显示出更强的特异性杀伤能力。本研究成功构建了经microRNA敲低PD-1的新型慢病毒载体并验证了其转导效率的优越性,以及基于此载体表达的microRNA可高效地沉默PD-1;且应用此载体的CAR-T细胞能发挥更强的杀伤活性,从而为后续该CAR-T细胞治疗表达PD-L1的肿瘤奠定基础。 相似文献
998.
长春花(Catharanthus roseus)对热带珊瑚岛生理生态适应性研究 总被引:1,自引:0,他引:1
长春花(Catharanthus roseus)是夹竹桃科的一种亚灌木植物,具有重要的药用价值和观赏价值,在前期的试验性种植中,发现长春花对热带珊瑚岛环境有很强的适应性。为了探讨长春花对热带珊瑚岛环境的生理生态适应性,该文以移植到热带珊瑚岛的长春花和生长于海南省文昌市苗圃的长春花为研究对象,对其叶片的形态解剖结构、生理学特征、营养元素含量等进行了分析。结果表明:(1)与苗圃生长的长春花和其他耐胁迫的植物相比,移植到热带珊瑚岛上的长春花具有叶片厚、栅栏组织发达、比叶面积小等形态解剖特征,这些特征有利于其光能吸收、水分储存和对环境资源的利用。(2)长春花的超氧化物歧化酶(SOD)、过氧化物酶(POD)和过氧化氢酶(CAT)活性较高,表现出较强的抗氧化性和抗胁迫能力。(3)长春花的叶绿素a和叶绿素b含量较低,可以减少过多的光能进入叶绿体光合系统,防止过剩的光能对光合系统产生伤害。(4)热带珊瑚岛土壤养分含量低,但生长在岛上的长春花叶片的营养元素含量高,表现出很强的养分吸收和利用能力。因此,长春花对干旱、贫瘠等恶劣生境具有很好的适应能力,可以作为热带珊瑚岛植被恢复工具种。 相似文献
999.
狭叶坡垒(Hopea chinensis)为常绿乔木,是我国热带季雨林的代表树种之一。它树型优美,具一定的耐寒性,是我国珍稀濒危保护植物。为阐明其传粉生物学特征和迁地保护的繁殖潜力,该文在引种地桂林植物园对狭叶坡垒的开花物候、花尺寸和花气味进行了观察和测量,运用杂交指数(OCI)、花粉胚珠比(P/O)、花粉活力、柱头活性检测和人工自交等方法对其繁育系统进行了检测,观察了访花昆虫并检验了其传粉效率,通过人工异交检验了繁殖潜力和可能的传粉限制。结果表明:(1)桂林植物园内狭叶坡垒的花期为7月底到9月底,持续60 d左右,一个花序花期约12 d,单花期约3 d,花朵开放时间为17:00—18:45。(2)雌雄同熟,雌蕊空间位置高于雄蕊,高花粉活力和高柱头可授性出现时间基本一致。(3)OCI 等于4,P/O为10 788±984。(4)无自动自花授粉能力且自交不亲和,自然条件下和异交授粉能坐果且坐果率无显著差异。(5)蕈蚊是狭叶坡垒唯一的传粉者。(6)主要花香成分为β-榄香烯、(E)-7,11-二甲基-3-亚甲基-1,6,10-十二碳三烯和1-石竹烯。综上所述,狭叶坡垒繁育系统为异交,在迁地保护地需要蕈蚊作为传粉者,能坐果并得到成熟种子,不存在授粉限制。 相似文献
1000.
贮食行为是动物应对食物资源的季节性和不可预测性变化的一种适应性生存策略。在群居性贮食动物中,同胎个体常表现出不同的贮食水平,而关于贮食行为与动物的个性和代谢水平之间的关系尚缺乏研究。本文以长爪沙鼠(Meriones unguiculatus)为对象,在筛选出具有高贮食和低贮食行为特征个体的基础上,比较了两组动物的个性特征(勇敢行为和探索能力)、静止代谢率、血清甲状腺激素水平、贮食期间的运动距离和贮食后的平均每日代谢率等。结果发现:高、低贮食长爪沙鼠的数量各占49%(22/45)和47%(21/45),两组动物之间的个性特征、静止代谢率和血清甲状腺激素均没有显著差异。在贮食期间高贮食个体的运动距离显著高于低贮食个体,且在停止贮食后,高贮食个体的平均每日代谢率显著低于低贮食个体。这些结果表明,在室内条件下,长爪沙鼠的贮食量高低与个性和静止代谢率无关,但高贮食个体会在停止贮食后降低其总能量消耗,以补偿贮食过程中的高能量代价。 相似文献