Monospecific antibodies against a synthetic peptide predicted from the alpha-3 nicotinic receptor cDNA inhibit binding of [3H]nicotine to rat brain nicotinic cholinergic receptor

Polyclonal antibodies were raised against a synthetic decapeptide (designated S3) predicted from a segment of the alpha-3 subunit cDNA (amino acid residues 130-139) encoding the rat brain nicotinic cholinergic receptor. This segment was selected because it may be proximate to the nicotine/acetylcholine-binding site of the receptor (1). By radioligand binding assays and sucrose density gradient centrifugation, these monospecific antibodies were shown to inhibit the binding of [3H]nicotine to both the large molecular weight rat brain receptor (240 kDa) and to an SDS-disaggregated nicotine-binding subunit species (80 kDa), in a dose-dependent manner. The neutralizing effect of the anti-S3 antibodies supports the view that this region of the protein is closely related to the agonist binding site.     

Acquired immunity in rats against Angiostrongylus cantonensis infection

Acquired immunity against Angiostrongylus cantonensis was induced by immunizing rats with somatic antigens from fifth-stage larvae and adult worms and live third-stage larvae. Rats immunized twice had significantly fewer worms than rats immunized three times. Fewer worms were recovered from rats immunized with 200 live third-stage larvae than from any other groups. Rats immunized with somatic antigens had higher enzyme-linked immunosorbent assay (ELISA) antibody levels than rats immunized with live larvae. Rats immunized with live third-stage larvae of Angiostrongylus cantonensis were more strongly protected against challenge infections (62-92%) than rats immunized with antigens extracted from fifth-stage larvae (0-30%) and adult worms (11-24%).     

大鼠缰核与下丘脑外侧区在调节心血管活动方面的机能联系

电刺激大鼠下丘脑外侧区（LH）,动脉压明显升高,心率加快,在刺激电极同侧缰核（Hb)内微量注射盐酸利多卡因、电刺激LH引起的升压反应可被阻断38.9%,心率增快反应可被阻断44.4%,双侧Hb内微量注射盐酸利多卡因,电刺激LH引起的升压反应可被阻断40.7%,心率增快反应可被阻断41.2% ,单侧或双侧Hb内微量注射人工脑脊液均不能阻断电刺激LH引起的心血管反应。电刺激大鼠Hb,动脉压明显升高,心率无明显改变,在刺激电极同侧LH内微量注射盐酸利多卡因,电刺激Hb引起的升压反应可被阻断63.2%,双侧LH内微量注射盐酸利多卡因,电刺激Hb引起的升压反应可被阻断62.6%,单侧或双侧LH内微量注射人工脑脊液均不能阻断电刺激Hb引起的心血管反应。本实验提示Hb与LH在调节心血管活动方面有协同作用。     

A partial map of the barley genome incorporating restriction fragment length polymorphism, polymerase chain reaction, isozyme, and morphological marker loci

Nine low copy number genomic DNA clones, a ribosomal sequence, and seven cDNA clones were found to identify polymorphisms in cultivated barley (Hordeum vulgare L.). An F2 population consisting of 100 plants was produced from a cross between a high-yielding two-rowed feed barley cultivar and a genetic marker stock homozygous for nine recessive and one dominant morphological marker genes. Through the use of these 10 well-distributed marker genes, five previously mapped isozyme loci, and two storage-protein loci, the approximate recombinational location for each of 17 restriction fragment length polymorphism loci was estimated. One clone, pMSU21, identified variation that appeared to be the result of a small insertion-deletion event that differentiated two-rowed and six-rowed genotypes. This difference was characterized, and one allele was sequenced. Oligonucleotide primers that flanked the insertion-deletion event were synthesized, and DNA samples from the F2 population were subjected to polymerase chain reaction sequence amplification. The variation identified by this technique was determined to be allelic to the variation identified using pMSU21 in Southern blot analysis. Maps of previously undescribed informative clones are included.     

草蛉科纳草蛉属研究（Ⅱ）—六新种记述（脉翅目：草蛉科）

本文是纳草蛉属Navasius研究续报(研究Ⅰ见“动物分类学报”第15卷第3期)。报道了纳草蛉属6新种:跃纳草蛉N.igneus sp.nov.,冠纳草蛉N.lophophorus sp.nov.,黑角纳草蛉N.nigricornutus sp.nov.显沟纳草蛉N.phantosulcus sp.nov.康纳草蛉N.sanus sp.nov.及三齿纳草蛉N.tridentatus sp.nov.。     

四川省米虾属一新种的描述（十足目：匙指虾科）

本文报道的刺肢米虾,新种Caridina spinipoda sp. nov。是采自四川绵竹泉水溪流中的二个雄性个体。其形状很似叶状米虾Caridina babaultioides Yu,但它第一腹肢内肢的结构非常特殊,因其周缘排列有既长又粗的刺而命名。     

木耳属种间杂交技术研究 Ⅰ.木耳菌丝原生质体的形成与再生

本文比较了不同酶液、渗透压稳定剂、酶解温室及菌丝培养基成份等因素对木耳属(Auricularia)中木耳(Auricularia auricula)和毛木耳(Auricularia polytricha)菌丝释放原生质体的作用及影响。用0.5%纤维素酶加0.5%蜗牛酶的混合酶液,以0.6M的MgSO_4为稳定剂,在34℃下可自两种菌丝体获得大量原生质体。对原生质体再生条件的研究表明,纤维二糖和菌丝体培养物浸提物对再生有明显促进作用,再生率达20%左右。本文还用VBL型荧光增白剂观察了菌丝脱壁以及原生质体细胞壁再生的过程。     

去甲肾上腺素在大鼠缰核引起的心血管效应及其机制

在乌拉坦麻醉的大鼠,研究了去甲肾上腺素(NE)在缰核(Hb)引起的心血管效应及其机制。Hb内微量注射NE使平均动脉压和心率呈剂量依赖性增加。用α受体阻断剂酚妥拉明预处理Hb,可明显减弱NE在Hb引起的心血管效应,但β受体阻断剂心得安或生理盐水不引起任何影响。Hb内微量注射海人酸使平均动脉压和心率明显增加,但Hb内微量注射利多卡因则不能引起明显的心血管效应。上述结果表明,Hb内NE在调节心血管活动中起重要作用,而这种效应可能是通过激活α受体使Hb兴奋的结果。     

NO—样松驰因子在大鼠止血带休克中的作用

为探讨NO-样松驰因子(NO-LRF)在休克中的效应及其病理生理意义,本工作在大鼠止血带休克(ToS)模型上发现离体灌流的主动脉环对去甲肾上腺素的反应性降低,组织cGMP含量增加。用NO前体L-精氨酸(L-Arg)或NO合成阻断剂L-NNA,可溶性乌苷酸环化酶抑制剂亚甲蓝灌流,分别增强或减轻ToS动物主动脉的上述变化,而且这些药物的作用不受血管内皮是否存在的影响。实验结果提示非内皮细胞源的NO-LRF是引起ToS动物血管低反应性的因素之一。体内实验表明 L-Arg治疗缓解,而L-NNA恶化ToS病程,提示NO-LRF可能参与机体的适应保护机制。     

囟土白蚁蚁后卵巢管数量、蚁巢体积与蚁群中各蚁型数量的相关性

囟土白蚁Odontotermes fontanellus Kemner是安徽为害林木、水库堤坝的主要蚁种.研究结果表明:1.在一定的巢龄范围内,随着蚁后身体增长,卵巢管数量增多,一侧的卵巢管最多可达3726条.2.蚁后体长与工蚁数、兵蚁数、幼蚁数、整巢蚁数之间成幂函数关系.3.卵巢管数与蚁后体长、工蚁数、兵蚁数、幼蚁数、整巢蚁数之间成幂函数关系,4,蚁巢体积(y)与蚁后体长(X₂)、兵蚁数(X₄)、的关系式为:y=6.687e^0.0566X₂+0.6741X₄