一种重组人α1型干扰素突变体IFN-α1/86D的纯化与鉴定

本文研究了重组人α1型干扰素突变体IFN-α1/86D在摇瓶培养和分批发酵培养的重组大肠杆菌DH5α株中的表达动态,并采用阳离子交换层析和抗α1型干扰素单克隆抗体亲和层析的两步流程对其进行了纯化,得到SDS-PAGE纯及高效液相层析(HPLC)纯的IFN-α1/86D产品,共比活性为2.3×10~7单位/毫克蛋白。N端氨基酸序列测定的结果表明,纯化产品的纯度在95％以上,但其N端不均一,产品中含有两种N端序列正确的IFN-α1/86D活性分子,即约75％为去Met分子和约25％为带Met分子。     

黄瓜子叶扩张与细胞分裂素的关系

BA(benzyl adenine)专一性地促进离体黄瓜子叶的扩张。为了研究 BA 的作用机理,我们采用间接 EIISA 和 HPLC 的方法测定了子叶扩张过程中内源玉米素(Z)和玉米素核苷(ZR)含量的变化。离体黄瓜(Cucumis sativus,津研4号)子叶用10mg/l 的 BA 培养,72小时之后,处理子叶鲜重的增加比对照高70%。Z+ZR 在 BA 处理的子叶中有大量的积累。结果表明 BA 可能诱发了黄瓜子叶中的细胞分裂素生物合成和代谢的某些基因。     

细胞分裂素在离体蒜苔内的运转

离体蒜苔饲喂带~3H 标记的6-苄基腺嘌呤(BA)后在暗中25℃下放置,分别在第3、5、10、15、20天取样,进行放射性物质的分布分析。结果表明:(1)BA 能沿着苔茎大量、长距离地上运,并在顶端的珠蒜中积累;(2)BA 的这种运转具有很强的向顶极性;(3)这种运转是一个平缓而稳定的过程,珠蒜中 BA 的积累与时间成较好的线性关系;(4)顶端的珠蒜对 BA 的上运是必不可少的。根据以上结果,本文对蒜苔内 BA 的运转机理及意义进行了讨论。     

The Ethanol Extract of Syringa oblata Heartwood,a Mongolian Folk Medicine Containing Major Lignans,Exerts Analgesic and Sedative Effects on Mice

The heartwood of Syringa oblata Lindl. (SO) is one of Mongolian folk medicines to treat insomnia and pain, while its pharmacological evaluation and underlying mechanism remain unclear. In this study, the sedative effect of ethanol extract of SO (ESO) was evaluated with the locomotor activity test and the threshold dose of pentobarbital sodium-induced sleep test in mice, and the hot plate test, acetic acid-induced writhing test, and formalin test in mice were used to evaluate its analgesic effect. The underlying mechanism of ESO analgesia was explored by RT-PCR and western blot analysis, which is associated with the regulation of the NF-κB signaling pathway. Besides, the main constituents of ESO were characterized by LC/MS data analysis and comparison with isolated pure compounds. The current findings brought evidence for clinical application and further pharmacological and phytochemical studies on SO.     

中国汉族人群间-α-胰蛋白酶抑制因子(ITI)的遗传多态性

我们建立了测定间-α-胰蛋白酶抑制因子遗传表型的聚丙烯酰胺凝胶等电聚焦免疫固定技术,应用这种方法对居住在成都地区的汉族群体进行了群体研究和家系调查,结果表明中国汉族人群间-α-胰蛋白酶抑制因子具有遗传多态性,它有希望成为法医血液遗传学新的遗传标记。     

Development of acute thermotolerance in L929 cells: lack of HSP28 synthesis and phosphorylation.

We investigated the correlation between the development of acute thermotolerance and the phosphorylation, synthesis, and expression of the HSP28 family in murine L929 cells. Following heating at 43 degrees C for 30 min, thermotolerance developed rapidly in exponential-phase cells and reached its maximum 4-9 h after heat shock. Maximal thermal resistance was maintained for 24 h and then gradually decayed. However, heat-induced phosphorylation of HSP28 was not detected. Furthermore, HSP28 synthesis during incubation at 37 degrees C for 12 h following heat shock was not detected by [3H]-leucine labeling followed by two-dimensional polyacrylamide gel electrophoresis. In addition, Northern blots failed to demonstrate expression of the HSP28 gene. Unlike HSP28, the expression of constitutive and inducible HSP70 genes, along with the synthesis of their proteins, was observed during incubation at 37 degrees C after heat shock. These results demonstrate that HSP28 synthesis and its phosphorylation are not required to develop acute thermotolerance in L929 cells.