Chromosomal assignment of 46 brain cDNAs.

Expressed sequence tags (ESTs) have been obtained from several hundred brain cDNAs as an initial effort to characterize expressed brain genes. These ESTs will become tools for human genome mapping and they will also provide candidate causative genes for inherited disorders affecting the central nervous system. We have developed a procedure for the rapid chromosomal assignment of these ESTs: cDNA sequences are first analyzed by a computer program to determine regions likely not to be interrupted by introns in the genomic DNA. A pair of oligonucleotide primers is then designed to amplify this region by the polymerase chain reaction using DNA template from human-rodent somatic cell hybrid chromosomal panels. The chromosomal assignment of the cDNA is determined by studying the segregation of the amplified products in these panels. In this paper we describe the mapping of 46 brain ESTs, as well as observations on the amplification of rodent sequences.     

Pollen-specific expression of theArabidopsis thaliana α1-tubulin promoter assayed by β-glucuronidase,chloramphenicol acetyltransferase and diphtheria toxin reporter genespromoter assayed by β-glucuronidase,chloramphenicol acetyltransferase and diphtheria toxin reporter genes

We have characterized the promoter specificity of theArabidopsis thaliana α₁-tubulin (α ₁-tub) gene by studying expression patterns of gene fusions between the 2.2 kbp 5′ upstream region of theα ₁-tub gene and each of three different reporters: chloramphenical acetyltransferase, β-glucuronidase or the diphtheria toxin chain A gene. Analysis of transgenic tobacco andArabidopsis plants carrying the transgene showed that the chloramphenicol acetyltransferase and β-glucuronidase activities were not detected in any vegetative or reproductive organs except mature pollen. Transgenic tobacco plants carrying the diphtheria toxin chain A gene under the control of theα ₁-tub promoter were of normal phenotype but seed fertility was drastically reduced. Furthermore, the transgene could not be transmitted to the next generation through pollen, supporting the observation that theα ₁-tub promoter is active only in pollen. It was observed that the promoter activity was most active in mature pollen and decreased significantly duringin vitro pollen germination, indicating that the promoter is inactive or subdued in germinating pollen. The promoter activity was not affected by various plant growth hormones during pollen maturation.     

大仓鼠肥满度的研究

本文依公式K=100W/L~3计算了大仓鼠肥满度。统计结果表明,雌性肥满度大于雄性肥满度。肥满度随年龄增长而减小。不同作物类型样地中大仓鼠的肥满度变化不明显。不同季节肥满度有一定的变化,主要与繁殖和气候有关。肥满度与种群密度之间不存在明显的对应关系。     

雄貉睾丸宽度和血清睾酮水平的季节性变化及其与某些繁殖特性的关系

作者测定并分析了43只雄貉睾丸宽度、血清睾酮水平的季节性变化。结果表明:睾丸宽度和血清睾酮水平呈明显的年周期季节性变化(p<0.01)。秋分(9月)时,睾丸宽度开始增大(p<0.05 );血清睾酮水平在10月开始升高(p<0.05)。各月雄貉的平均睾酮水平与平均睾丸宽度是极显著的正相关(r=0.83,p<0.01 n=11)。雄貉繁殖季节初期,血清睾酮水平与其首、末次的交配日期呈显著负相关(r=-0.525和r=-0.476,p<0.05,n=19)。     

中国乌头属属下等级数量分类的初步研究

本文取中国乌头属50种(含变种)植物,选取形态学性状46个、化学性状8个、组织学性状5个、生态学性状1个,运用数量分类方法,对该属进行属下等级的分类研究。根据数学分析结果,确定了树系图中变种、种、系、亚属的分级界线,并在聚类分析的基础上,进一步探讨了各等级类群的划分。     

过硫酸铵-N,N,N′,N′-四甲基乙二胺体系产生的氧自由基及其清除的研究

应用ESR和自旋捕集相结合的技术直接测定了过硫酸铵—N,N,N′,N′-四甲基乙二胺(AP-TEMED)体系产生的氧自由基,经计算机波谱模拟和计算波谱参数证实该体系产生的氧自由基是O_2~-和·OH。并用维生素C、茶多酚、超氧化物歧化酶等氧自由基清除剂,从聚丙烯酰胺凝胶法、化学发光法和脂质过氧化法不同角度研究了AP-TEMED体系在自由基研究方面的应用意义。     

用2DNMR鉴定倍半萜多元酯的结构

利用2DNMR结合其他波谱方法从苦皮藤种子油中鉴定了2个新倍半萜多元酯,它们分别是1α-苯甲酰氧-8β-烟酰氧-9α、11β-2乙酰氧-β-二氢沉香呋喃,1β-苯甲酰氧-2α、8β、9α、11β-4乙酰氧-β-二氢沉香呋喃,命名为苦皮藤酯2和3。     

毛枝南蛇藤根皮中南蛇藤素的分离鉴定及含量测定

从卫矛科南蛇藤属植物毛枝南蛇藤(Celastrus hookeri Prain)根皮中首次分离出一个红色立方体形结晶,经紫外、红外、高分辨质谱和~(13)C核磁共振谱分析,并与有关文献数据对照,确认是南蛇藤素。作者用薄层扫描和高效液相两种方法初步测定了毛枝南蛇藤根皮和根的木栓化表皮的南蛇藤素含量,结果表明南蛇藤素主要集中在根的木栓化表皮,含量在12%以上,而在整个根皮中的含量大约为1%。     

青海省雏蝗属一新种记述：（直翅目：蝗总科：网翅蝗科）

在整理由青海省采得蝗虫标本时,发现1新种,记述如下。模式标本保存于山东大学生物系。青海雏蝗Chorthippus qinghaiensis,新种(图1—8) 雄:体小型。头部短于前胸背板。头顶平,侧缘明显隆起,顶锐角形。头侧窝狭长方形,长为宽的3.3倍。颜面向后倾斜,颜面隆起纵沟较浅,具刻点,侧缘在中眼处略狭。触角丝状,中段一节长为宽的1.4倍。复眼较小,卵圆形,纵径为横径的1.3倍,