全文获取类型
收费全文 | 3986篇 |
免费 | 506篇 |
国内免费 | 1659篇 |
出版年
2024年 | 19篇 |
2023年 | 108篇 |
2022年 | 174篇 |
2021年 | 212篇 |
2020年 | 178篇 |
2019年 | 218篇 |
2018年 | 155篇 |
2017年 | 133篇 |
2016年 | 168篇 |
2015年 | 260篇 |
2014年 | 323篇 |
2013年 | 260篇 |
2012年 | 404篇 |
2011年 | 427篇 |
2010年 | 293篇 |
2009年 | 315篇 |
2008年 | 326篇 |
2007年 | 339篇 |
2006年 | 289篇 |
2005年 | 275篇 |
2004年 | 184篇 |
2003年 | 175篇 |
2002年 | 166篇 |
2001年 | 147篇 |
2000年 | 115篇 |
1999年 | 76篇 |
1998年 | 42篇 |
1997年 | 38篇 |
1996年 | 37篇 |
1995年 | 33篇 |
1994年 | 33篇 |
1993年 | 18篇 |
1992年 | 30篇 |
1991年 | 15篇 |
1990年 | 13篇 |
1989年 | 14篇 |
1988年 | 19篇 |
1987年 | 24篇 |
1986年 | 14篇 |
1985年 | 14篇 |
1984年 | 7篇 |
1983年 | 8篇 |
1982年 | 9篇 |
1981年 | 5篇 |
1980年 | 7篇 |
1979年 | 5篇 |
1978年 | 5篇 |
1974年 | 4篇 |
1973年 | 4篇 |
1972年 | 4篇 |
排序方式: 共有6151条查询结果,搜索用时 171 毫秒
21.
22.
Compartmentalization of SHC, GRB2 and mSOS, and hyperphosphorylation of Raf-1 by EGF but not insulin in liver parenchyma. 总被引:15,自引:1,他引:14 下载免费PDF全文
Rat liver parenchyma harbors equal numbers of epidermal growth factor (EGF) and insulin receptors. Following administration of a saturating dose of EGF (10 micrograms/100 g body weight), there was a rapid (t1/2 approximately 1.1 min) internalization of receptor coincident with its tyrosine phosphorylation at residue 1173 and receptor recruitment of the adaptor protein SHC, its tyrosine phosphorylation and its association with GRB2 and the Ras guanine nucleotide exchange factor, mSOS, largely in endosomes. This led to a cytosolic pool of a complex of tyrosine-phosphorylated SHC, GRB2 and mSOS. It was demonstrated that these constituents were linked to Ras activation by the characteristic decrease in Raf-1 mobility on SDS-PAGE, which was maintained for 60 min after a single bolus of administered EGF. While insulin administration (15 micrograms/100 g body weight) led to insulin receptor beta-subunit tyrosine phosphorylation and internalization, there was little detectable tyrosine phosphorylation of SHC, recruitment of GRB2, association of a complex with mSOS or any detectable change in the mobility of Raf-1. Therefore, in normal physiological target cells in vivo, distinct signaling pathways are realized after EGF or insulin receptor activation, with regulation of this specificity most probably occurring at the locus of the endosome. 相似文献
23.
The importance of spontaneous lac host-trees for preserving the fragile agroecosystem of the main lac producing areas of China, located in the tropical part of Yunnan province, is analyzed. In upland fields, the fire-resistant vegetation consists mainly of these trees. Farmers deliberately preserve young sprouts during the cultivation cycle. This benefits both soil fertility and lac production. Host-trees, mainly Dalbergia and Ficus, form a range of multipurpose trees which although limited to some ten species allows maximum use of the heterogeneities of the village territory. Finally, lac host-trees are associated with some environmental values important to farmers. This circumstance contributes to the sustainability of lac host-tree agroforestry in spite of current problems in the lac market. 相似文献
24.
Expression and characterization of virus-like particles containing rubella virus structural proteins. 总被引:7,自引:1,他引:6 下载免费PDF全文
Rubella virus (RV) virions contain two envelope glycoproteins (E1 and E2) and a capsid protein (C). Noninfectious RV-like particles (VLPs) containing three structural proteins were expressed in a BHK cell line (BHK-24S) by using an inducible promoter. These VLPs were found to resemble RV virons in terms of their size, their morphology, and some biological activities. In immunoblotting studies, VLPs were found to bind similarly to native RV virions with 10 of a panel of 12 RV-specific murine monoclonal antibodies. Immunization of mice with VLPs induced specific antibody responses against RV structural proteins as well as virus-neutralizing and hemagglutination-inhibiting antibodies. After immunization of mice with VLPs, in vitro challenge of isolated lymphocytes with inactivated RV and individual RV structural proteins stimulated proliferation. Our data suggest the possibility of using VLPs as immunogens for serodiagnostic assays and RV vaccines. 相似文献
25.
湿螨属四新种记述及中华湿螨再记述:(蜱螨亚纲:湿螨科) 总被引:1,自引:1,他引:0
本文记述湿螨属水螨四种新种:郭氏湿螨Hygrobates gousi sp.nov.,贵州湿螨H.guiahouensis sp.nov.,墨缘湿螨H.atrovirens sp.nov.和兴义湿螨H.xinyiensis sp.nov..文中扼述了它们与近似种的区别。另外。还对中华湿螨H.sinensis Uchide Imamura作了再描述。 相似文献
26.
埃及血吸虫毛蚴表皮板共有4排,各排数目为6:9:4:3。除第1排之外,其他各排的表皮板均由无纤毛的嵴所分隔,在第1与第2排之间的环嵴中,除第1排各板底边中央小凹处各有1感受器外,尚可见4对感受器。第2与第3排的环嵴内可见16—20个感受器。此外,于体之两侧第3排表皮板间各见1个排泄孔。顶突似六边形,于其两侧各有1个侧腺开口,其内侧可见14个感受器。本文在讨论中与前人对本虫毛蚴表皮板的描述做了简单比较。 相似文献
27.
鸡马立克氏病病毒B抗原片段在大肠杆菌中表达 总被引:1,自引:0,他引:1
鸡马立克氏病病毒BamHI基因文库I3质粒中含有编码B抗原膜外Domain的DNA序列。经ScaI和SphI双酶酶解I3质粒,分离获得764bpDNA片段,并克隆进M13mp19中。DNA序列测定分析表明克隆片段为MDV-B抗原基因的494-1258bp部分序列。进一步分离NcoI-HindIII部分基因片(530bp),克隆于PLPromoter控制下的含有修饰型cIts857基因的表达载体中, 相似文献
28.
Transthyretin(TTR) gene was highly expressed in normal liver and it has been found to be deleted in part of DNA samples from human hepatic cancer.Its mRNA expression was suppressed in most hepatoma samples.In order to study the biological effect of TTR gene on the growth of hepatoma cells,a recombinant vector containing TTR cDNA was constructed by pCMV,then it was transfected into hepatoma cell lines SMMC-7721 and Q3.It has been demonstrated that the inhibition of growth rate of TTR cDNA transfected hepatoma cells was about 50% in strength compared with that of the control.This inhibition was further enhanced when the transfected hepatoma cells were treated with all-trans retinoic acid.Hepatoma cells of cell lines PLC/PRF/5,SMMC-7721 and Q3 as well as hepatoma cells SMMC-7721 transfected with pCMV or pCMV-TTR were analyzed for TTR expression by Northern hybridization.The low level of TTR expression was found in both hepatoma cell lines and in SMMC-7721 cells transfected with pCMV alone.However,a remarkable TTR mRNA expression was observed in hepatoma SMMV-7721 cells transfected with pCMV-TTR.It seems possible that TTR gene might be a candidate of cancer suppressor gene for human hepatic cancer. 相似文献
29.
The influence of temperature, shoot age, and medium on gall induction by Subanguina picridis on Russian knapweed (Acroptilon repens) was examined in vitro. The optimal temperature for gall formation was 20 C. Gall induction was delayed as the temperature decreased, and decreased as shoot age increased. Bud primordia (0-day-old shoots and 5-day-old shoots) with an average length of 4.2 mm and 7.9 mm were the most suitable tissues for nematode development and gall formation. Gall formation was more effective on B5G medium than on MSG. Young shoots under slow growth were most suitable for mass rearing of S. picridis. 相似文献
30.
Depression of Neuronal Protein Synthesis Initiation by Protein Tyrosine Kinase Inhibitors 总被引:2,自引:1,他引:1
Abstract— Growth factors stimulate cellular protein synthesis, but the intracellular signaling mechanisms that regulate initiation of mRNA translation in neurons have not been clarified. A rate-limiting step in the initiation of protein synthesis is the formation of the ternary complex among GTP, eukaryotic initiation factor 2 (elF-2), and the initiator tRNA. Here we report that genistein, a specific tyrosine kinase inhibitor, decreases tyrosine kinase activity and the content of phosphotyrosine proteins in cultured primary cortical neurons. Genistein inhibits protein synthesis by >80% in a dose-dependent manner (10–80 μg/ml) and concurrently decreases ternary complex formation by 60%. At the doses investigated, genistein depresses tyrosine kinase activity and concomitantly stimulates PKC activity. We propose that a protein tyrosine kinase participates in the initiation of protein synthesis in neurons, by affecting the activity of elF-2 directly or through a protein kinase cascade. 相似文献