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31.
高翔蜉属一新种:蜉蝣目:扁蜉蝣科 总被引:1,自引:1,他引:0
本文记述采自湖南省大庸市高翔蜉属一新种。该种以其阳茎叶外侧面具有一向前弯曲的钩状突起为显著特征,可区别于本属其他种类。 相似文献
32.
研究一系列有明确意义改性胰岛素的结构将有益于新型胰岛素分子的设计。经蛋白质工程制备的AspA21—人胰岛素(ADHI)基本保持了生物活力,而在酸性溶夜(pH3—4)中的稳定性比天然胰岛素在中性溶液中高5—10倍。本文报道ADHI的结晶、初步晶体学研究及中等分辨率衍射数据的收集。ADHI晶体属R3空间群:a_H=b_H=82.72埃,c_H=34.02埃。 相似文献
33.
给完整的及切除肾上腺的雌性 Wistar 大鼠分別注射地塞米松、去氧皮质酮或地塞米松加去氧皮质酮;冷酚法提取心房总 RNA,用α-~(32P)标记的大鼠心房肽 cDNA 探针与之杂交。完整大鼠接受地塞米松和切除肾上腺后接受地塞米松加去氧皮质酮的大鼠,心房肽基因转录产物增加2倍,其余组无显著变化。结果提示糖皮质激素可促进心房肽基因表达,但此作用依赖于盐皮质激素的同时存在,单纯盐皮质激素不能增强该基因的表达。 相似文献
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35.
Various daily photon doses (daily net number of photons perunit area), provided by varying both photon flux density andphotoperiod, were applied to dormant seed populations of fourgraminaceous species tested for germination in alternating-temperatureregimes on filter papers moistened with either water or a solutionof potassium nitrate. In all four species germination was dependentupon daily photon dose, the product of photon flux density andduration, and quantified by linear relations between the probitof percentage germination and the logarithm of the daily photondose determined by probit analysis. Photon flux density andphotoperiod only influenced germination indirectly as factorsof daily photon dose, except in Echinochloa turnerana (Domin)J.M. Black at high daily photon doses where lower germinationwas observed in continuous light than in 8 h d1 photoperiod.The low energy reaction, in which increasing dose promotes seedgermination, was observed in Echlnochloa turnerana and Eragrostistef(Zucc.) Trotter but not in the populations of Brachiariahumidicola (Rendle) Schwcickerdt and Panicum maximum Jacq. usedin this study. The high irradiance reaction, in which increasingthe photon dose reduces germination, was, however, observedin all four species. In Eragrostis tef potassium nitrate influencedthe response of seed germination to light, but in the remainingspecies its influence on this relationship was either additive(Panicum maximum) or nil (Brachiaria humidicola and Echinochloaturnerana) in the alternating-temperature regimes used in thisstudy. The implications of the quantal response to photon dosefor devising optimal light regimes to be applied in laboratorytests intended to maximize seed germination are discussed. Key words: Light, seed germination, seed dormancy, grasses 相似文献
36.
Genomic DNA sequence for human C-reactive protein 总被引:12,自引:0,他引:12
K J Lei T Liu G Zon E Soravia T Y Liu N D Goldman 《The Journal of biological chemistry》1985,260(24):13377-13383
The gene for the prototype acute phase reactant, C-reactive protein, has been isolated from two lambda phage libraries containing inserted human DNA fragments using synthetic oligonucleotide probes. Nucleotide sequence analysis indicates that after coding for a signal peptide of 18 amino acids and the first two amino acids of the mature protein, there is an intron of 278 base pairs followed by the nucleotide sequence for the remaining 204 amino acids. The intron is unusual in that it contains on the positive strand a poly(A) stretch 16 nucleotides long and a poly(GT) region 30 nucleotides long which could adopt the Z-form of DNA. The nucleotide sequence reported here confirms the amino acid sequence of mature C-reactive protein as originally reported except that it codes for an additional 19 amino acids beginning at position 62. Thus DNA sequence analysis predicts that the mature protein consists of 206 amino acids rather than 187 as originally reported. The mRNA cap site is located 104 nucleotides from the start of the signal peptide and there is a 3' noncoding region 1.2 kilobase pairs in length. The gene has a typical promoter containing the sequences TATAAAT and CAAT 29 and 81 base pairs upstream, respectively, of the cap site. 相似文献
37.
38.
An improved DNA sequencing strategy 总被引:42,自引:0,他引:42
A modification of Hong's systematic DNA sequencing strategy is described. The original procedure has been simplified and transfectant yield increased. After DNase I limited cleavage in the presence of Mn2+, the single-cut linear DNA does not have to be separated from supercoiled or open circular DNA on an agarose gel. After ligation, the DNA is digested with a second restriction endonuclease for which a unique cleavage site resides between the insert and the first restriction endonuclease cutting site. The original intact DNA is linearized whereas the deleted subclone is not. The background is decreased to an undetectable level. This DNA sequencing strategy was tested on a 1.4-kb DNA fragment containing the araC regulatory gene from Erwinia carotovora. A set of subclones sufficient to sequence the fragment on both strands was produced in 2 days and the yield was at least 60-fold higher than in the original protocol. 相似文献
39.
Dr. Berend van der Lei Charles R. H. Wildevuur Paul Nieuwenhuis Engbert H. Blaauw Freark Dijk Caesar E. Hulstaert Izaäk Molenaar 《Cell and tissue research》1985,242(3):569-578
Summary The ultrastructure of a new type of vascular graft, prepared from a mixture of polyurethane (95 weight %) and poly-L-lactic acid (5 weight %), was examined six weeks after implantation into the abdominal aorta of rats. These microporous, compliant, biodegradable, vascular grafts function as temporary scaffolds for the regeneration of the arterial wall.Smooth muscle cells, covering the grafts, regenerated a neo-media underneath an almost completely regenerated endothelial layer (neo-intima). These smooth muscle cells varied in morphology from normal smooth muscle cells to myofibroblasts. They were surrounded by elastic laminae and collagen fibers.Macrophages, epithelioid cells, multinucleated giant cells, fibroblasts and capillaries were present in the disintegrating graft lattices. The epithelioid cells and multinucleated giant cells engulfed polymer particles of the disintegrating grafts.The regeneration of the endothelial and smooth muscle cells is similar to the natural response of arterial tissue upon injury. The presence of macrophages, epithelioid cells, multinucleated giant cells, fibroblasts and capillaries in the graft lattices resembles the natural response of tissue against foreign body implants. Both of these responses result in the formation of a neo-artery that possesses sufficient strength, compliance and thromboresistance to function as a small caliber arterial substitute.Supported by Grant nr. 82.042 from the Dutch Heart Foundation 相似文献
40.
Cloning of the pectate lyase genes from Erwinia carotovora and their expression in Escherichia coli 总被引:11,自引:0,他引:11
A hybrid cosmid coding for pectate lyase (PL) activity was identified from an Erwinia carotovora genomic library by an immunological screening method. A 7-kb DNA fragment was identified which codes for three proteins identical in size to proteins with PL activity purified from E. carotovora culture supernatants. The three proteins had apparent Mrs of 41, 44 and 44 X 10(3) as estimated by SDS-PAGE. None of the PLs were exported from Escherichia coli strain HB101 but all were found in the periplasmic space. Plant tissue was macerated by the PLs made in E. coli. 相似文献