NGFI-B targets mitochondria and induces cardiomyocyte apoptosis in restraint-stressed rats by mediating energy metabolism disorder

NGFI-B/Nur77/TR3, originally identified as an immediate-early gene rapidly induced by serum and growth factors, is a member of the steroid hormone nuclear receptor superfamily with no identified endogenous ligand. NGFI-B induces apoptosis in a number of cell lineages exposed to proapoptotic stimuli by directly targeting the mitochondria, inducing cytochrome c release. The present study was designed to determine the role of NGFI-B in cardiomyocytes of restraint-stressed rats. The NGFI-B content was increased in mitochondria and reduced in plasma as apoptosis increased. Analysis showed that NGFI-B induces cardiomyocyte apoptosis in restraint-stressed rats by mediating mitochondrial energy metabolism disorder. Several novel mitochondrial proteins, which correlate with NGFI-B, were reported in cardiomyocyte apoptosis of restraint-stressed rats. Five proteins associated with NGFI-B participate directly in mitochondrial energy metabolism. Studies of mitochondrial respiratory efficiency and ATP synthase activity strongly support the findings. These results provide significant information for comprehensively understanding the cellular mechanism of cardiovascular diseases.     

Fine-Scale Population Genetic Structure of Zhikong Scallop (Chlamys farreri): Do Local Marine Currents Drive Geographical Differentiation?

Marine scallops, with extended planktonic larval stages which can potentially disperse over large distances when advected by marine currents, are expected to possess low geographical differentiation. However, the sessile lifestyle as adult tends to form discrete "sea beds" with unique population dynamics and structure. The narrow distribution of Zhikong scallop (Chlamys farreri), its long planktonic larval stage, and the extremely hydrographic complexity in its distribution range provide an interesting case to elucidate the impact of marine currents on geographical differentiation for marine bivalves at a fine geographical scale. In this study, we analyzed genetic variation at nine microsatellite DNA loci in six locations throughout the distribution of Zhikong scallop in the Northern China. Very high genetic diversity was present in all six populations. Two populations sampled from the same marine gyre had no detectable genetic differentiation (F (ST) = 0.0013); however, the remaining four populations collected from different marine gyres or separated by strong marine currents showed low but significant genetic differentiation (F (ST) range 0.0184-0.0602). Genetic differentiation was further analyzed using the Monmonier algorithm to identify genetic barriers and using the assignment test conducted by software GeneClass2 to ascertain population membership of individuals. The genetic barriers fitting the orientation of marine gyres/currents were clearly identified, and the individual assignment analysis indicated that 95.6% of specimens were correctly allocated to one of the six populations sampled. The results support the hypothesis that significant population structure is present in Zhikong scallop at a fine geographical scale, and marine currents can be responsible for the genetic differentiation.     

膝部骨折合并腘动脉损伤23例临床分析

目的：回顾性分析膝部骨折合并腘动脉损伤的治疗方法和效果。方法：23例膝部骨折合并腘动脉损伤的患者,其中肢体严重缺血患者（远端动脉搏动消失,皮温下降,皮肤花斑或者苍白）13例,部分缺血患者（远端动脉搏动减弱,或者消失但有毛细血管充盈征）10例。腘动脉修复方法：端端吻合术4例,修补术5例,切开取栓术3例,对侧大隐静脉移植修复术10例。修复顺序：先修复血管再固定骨折8例,先固定骨折再修复血管14例,处理骨折前先建立临时性动脉内分流10例。结果：肢体存活19例,截肢4例。截肢者均为严重缺血患者,其中1例患者因严重骨折和广泛软组织损伤合并急性肾功能衰竭行I期截肢,3例患者因术后反复感染（1例合并肾功能不全）行Ⅱ期截肢。严重缺血的患者只有3/13例完全恢复,而部分缺血的患者有6/10例完全恢复。血管再通时间≥8h的患者只有4/13例完全恢复,而血管再通时间〈8h的患者有5/9完全恢复。结论：膝部骨折合并腘动脉损伤时,肢体缺血程度和缺血时间是影响患者康复的重要因素,术后感染仍是造成截肢的主要原因。     

低分子肝素对MPTP帕金森小鼠的治疗作用及可能机制

目的：研究1．甲基4-苯基-1,2,3,6-四氢吡啶（1-methy-4-phenyl-1,2,3,6-tetrahy-dropyridine,MPTP）帕金森病（PD）模型中小胶质细胞的激活情况,探讨低分子肝素对MPTP导致的小胶质细胞活化的抑制作用。方法：C57BL随机分成正常对照组、MPTP组、低分子肝素＋MPTP组。MPTP组腹腔注射MPTP（30mg／kgx7d）同时腹部皮下注射生理盐水,低分子肝素＋MPTP组在注射MPTP同时腹部皮下注射低分子肝素（1501U／kg·12hx7d）。各组于末次给药后予行为学测试,7d后免疫组化检测酪氨酸羟化酶（TyrosineHydroxylase,TH）阳性细胞。镀银染色观察小胶质细胞激活情况。结果：MPTP组较低分子肝素＋MPTP组爬竿时间明显延长,并出现更多非随意动作。低分子肝素＋MPTP组黑质部位TH阳性细胞数量高于MPTP组。MPTP组活化的小胶质细胞数量高于低分子肝素＋MPTP组。结论：低分子肝素通过抑制小胶质细胞的激活减少MPTP帕金森小鼠多巴胺能神经元的损伤,提示低分子肝素可能有延缓PD进程的作用。     

定量聚合酶链反应评估去白细胞滤器去除血液成分中巨细胞病毒的效果

为了评估目前常用的去白细胞滤器去除血液成分中人巨细胞病毒( HCMV) 的效果, 我们在献血员中筛选获得HCMV-IgG、pp65 皆阴性的血液, 分离外周血单个核细胞( PBMC) , 将HCMV 感染的人成纤维细胞与PBMC 共培养, 感染HCMV 的PBMC 经抗人成纤维抗体和抗CD45 磁珠2 次分选纯化后, 将CD45 + 单核细胞加回原全血中, 建立HCMV 潜伏感染的模型, 再用去白细胞滤器过滤。用实时聚合酶链反应( PCR) 定量测定过滤前、后血液样本中的病毒载量, 残留成纤维细胞所含的HCMV 通过定量反转录PCR( RT - PCR) 扩增脯-4-羟化酶exon12a mRNA 来校正。结果显示, 使用去白细胞滤器后, 每单位( 200 ml) 全血血液中, 白细胞的去除率为99. 98% 。全血中平均HCMV 病毒载量从3 742 拷贝/ μl 降至22. 57 拷贝/ μl, 降低2. 50 log10 , 说明去白细胞滤器虽然可明显减少全血中HCMV 病毒的载量, 但并不能完全去除病毒。     

糖尿病足的三种疗法比较及护理效应

目的:研究中西医结合治疗糖尿病足的临床疗效.方法:将146例糖尿病足患者按已接受的治疗方法分为中西医结合治疗组43例和中医药组49例和西医治疗组54例.西医组患者在降血糖的基础上给予营养神经、抗凝、扩张血管、改善微循环、抗感染等治疗,中西医治疗组在此基础上加用中医辨证施治.中医药组则辨证施治内服外用中药.结果:三种疗法疗效对比临床症状改善时间(d)中西医结合治疗组与其他组比较差异有显著性(P＜0.05).三组治疗后空腹血糖,糖化血红蛋白水平均有改善,其中西医组比中医组明显(P＜0.05).但中西医结合治疗组与其他组比较疗效更佳,统计学有显著性差异(P＜0.05).结论:中西医结合组治疗糖尿病足的疗效更好.     

多孔钽钉植入治疗早期股骨头缺血性骨坏死

目的:总结多孔钽钉植入治疗成人早期股骨头缺血性骨坏死(avascular necrosis of the femoral head,ANFH)的手术方法及临床疗效.方法:2008年3月-2009年2月,采用多孔钽钉植入治疗10例(共11髋)ANFH患者.男7例,女3例;年龄18-36岁,平均28岁.致病原因:创伤性2例,激素性2例,酒精性1例,特发性6例.单侧9例,双侧1例.左侧5髋,右侧6髋.根据1995年Steinberg分型:Ⅰ期3例3髋,Ⅱ期7例8髋.Harris评分为(52.3±1.2)分.病程11-26个月,平均15个月.术前术后影像学对比.结果:手术时间平均25min,平均出血量50ml.术后患者切1:2均Ⅰ期愈合,无感染等并发症发生.10例均获随访,随访时间7-14个月,平均11个月.患者术前疼痛及功能受限症状均明显缓解.术后Harris评分为(82.9±1.4)分,与术前比较差异有统计学意义(P<0.05);影像学显示术后坏死程度没有进展或改善.结论:多孔钽钉植入是治疗早期ANFH理想的微创手术疗法,特别是骨坏死处于Ⅰ期和Ⅱ期的年轻病人,既解除疼痛又可最大限度减少并发症,至少延缓甚至避免了关节置换,但远期疗效尚待大样本特别是多中心水平的长期随访观察.     

网络成瘾类型特点及成瘾原因述评

不同类型的网络成瘾在其需要、特点和成瘾机制上存在着差异,因此,本文对国内外关于网络成瘾的类型特点及不同成瘾类型的成瘾原因进行了述评并对后续研究进行展望,旨在为网络成瘾实施个体化的预防和干预方案提供借鉴,使成瘾者和网络使用者以健康、科学的态度合理使用网络,适应现实生活.