鲢、鳙、青、草鱼地理分布的研究

鲢、鳙、青、草鱼是我国最重要的四种淡水家养鱼类,自1958年人工繁育成功后,现已被各处广为引种养殖,其原来的自然分布区迄今尚无专文研究,为了避免将来无从查考,本文根据较早文献记载及有关鱼化石的研究等,对其自然分布区作了归纳,并对其分布规律作了较详的分析及说明。     

Human pescadillo induces large-scale chromatin unfolding

The human pescadillo gene encodes a protein with a BRCT domain. Pescadillo plays an important role in DNA synthesis, cell proliferation and transformation. Since BRCT domains have been shown to induce chromatin large-scale unfolding, we tested the role of Pescadillo in regulation of large-scale chromatin unfolding. To this end, we isolated the coding region of Pescadillo from human mammary MCF10A cells. Compared with the reported sequence, the isolated Pescadillo contains in-frame deletion from amino acid 580 to 582. Targeting the Pescadillo to an amplified, lac operator-containing chromosome region in the mammalian genome results in large-scale chromatin decondensation. This unfolding activity maps to the BRCT domain of Pescadillo. These data provide a new clue to understanding the vital role of Pescadillo.     

丹参愈伤组织的诱导及增殖效应

以丹参(Salvia miltiorrhiza bunge)的幼叶、茎、叶柄为外植体,接种于附加2,4—D、MM、Kr、Zr、6—BA及其组合的hIs固体培养基上,结果发现单独使用四种植物生长调节物质在一定浓度范围均有愈伤组织产生;最佳组合的诱导培养基为MS 2,4—D0．5mg/L 6—BA1．0mg/L。进一步研究发现,在黑暗和光照培养条件下,愈伤组织增殖呈“S”型,且生长周期均为30天。     

Facile synthesis of 4-substituted-4-aminopiperidine derivatives, the key building block of piperazine-based CCR5 antagonists

4-Substituted-4-aminopiperidine is an interesting structural motif found in a number of bioactive compounds. An efficient and convenient method for the synthesis of 4-differently substituted-4-aminopiperidine derivatives was described, employing isonipecotate as a starting material and Curtius rearrangement as a key step. The alkylation of isonipecotate could introduce various substituents at the 4-position of the piperidine ring. With this key building block, we are able to efficiently synthesize piperazino-piperidine based CCR5 antagonist in a highly convergent manner free of using toxic reagents such as diethylaluminum cyanide. The concise synthesis of a potent bioavailable CCR5 antagonist as HIV-1 entry inhibitor, Sch-350634 (1) was accomplished in excellent yield using N'-Boc-4-methyl-4-aminopiperidine 5a as a smart building block. The new methodology provides a facile and practical access to the piperazino-piperidine amide analogs as HIV-1 entry inhibitors.     

血管紧张素Ⅱ受体和钙蛋白酶在心肌重构中的作用

目的:探讨不同程度充血性心力衰竭(CHF)病人心肌组织血管紧张素Ⅱ受体(AT1、AT2)mRNA表达与钙蛋白酶和心功能的关系.方法:RT-PCR检测39例瓣膜病所致的不同程度心力衰竭病人与8例正常人心肌组织AT1和AT2 mRNA表达.免疫沉淀检测钙蛋白酶(u-calpain,m-calpain)蛋白表达.结果:瓣膜病所致心力衰竭病人心肌组织呈心肌重塑的病理改变.轻度心衰组AT1 mRNA表达较正常人高,中重度心衰组AT1mRNA表达明显降低,AT2mRNA表达差异无显著性,但AT1/AT2比值明显降低.u-calpain,m-calpain蛋白表达随心功能恶化而增加.结论:重度心衰病人心肌组织以AT2为主,钙蛋白酶系统处于激活状态进一步恶化心功能.     

自桑兰叶甲分离出的一种微孢子虫(Mic-Ⅰ)的研究

从广州郊区罗岗的桑园及菜地捕捉到的桑兰叶甲Mimastra cyanura Hope成虫体中分离到一种卵圆形的微孢子虫（简称Mic-Ⅰ）。孢子大小为(3.35±0.46)×(1.96±0.17) μm;孢子具单核、双核两种类型;极丝10～11圈;孢子表面抗原的血清学类型与家蚕微粒子虫Nosema bombycis孢子不同;在家蚕Bombyx mori体内以两种不同的生活史发育,发育过程符合变态孢虫属Vairimorpha微孢子虫发育特征。Mic-Ⅰ微孢子虫对家蚕具强病原性,对斜纹夜蛾Prodenia litura和小菜蛾Plutella xylostella也有感染能力。     

甘薯兼抗薯瘟病和蔓割病种质筛选鉴定

用甘薯瘟2个致病型菌株对557份甘薯品种(系)分菌系做盆苗和田间接种鉴定,并用蔓割病菌接种鉴定.结果表明,筛选出兼抗薯瘟病2个菌系的品种(系)31份,兼抗甘薯瘟和蔓割病的品种(系)有16份,其中泉薯860、泉薯854、金山93、榕选416、金山908、广薯88-70和泉薯853等7份与我省主栽抗病品种湘薯75-55(CK)相比,具有类似的双抗性能力,而其抗瘟能力更强.福薯87、林泗2号、金山57和湛93-16等4份对照品种只抗单个病害或兼抗单个菌系.     

The design of new molecular "light switches" for DNA

Two novel ruthenium(II) complexes, [Ru(pztp)2(phen)](ClO4)2 and [Ru(pztp)2(bpy)] (ClO4)2, have been synthesized and characterized by UV/Vis and 1H NMR spectroscopies and mass spectrometry. The MeCN solutions of both complexes display fluorescence that was found to be highly sensitive to the presence and concentration of water. The complexes behave like a "light switch" for DNA in that they do not luminesce in water but were "turned on" in the presence of DNA and show emission enhancement with the increase of DNA concentration. Their DNA binding behavior was also studied by absorption spectroscopy and viscosity measurements, which suggest that the DNA-complex interaction involves intercalation of the metal-bound pztp ligand into the base pairs of duplex DNA.     

蛱蝶翅鳞片的超微结构观察

对我国东北地区典型常见蛱蝶科15属20种蝴蝶翅鳞片的超微结构进行了扫描电镜观察。结果显示：蛱蝶翅鳞片形态上可分为窄叶形、阔叶形和圆叶形3种,鳞片长65～135 μm,宽35～85 μm,间距48～112 μm。蛱蝶翅鳞片的超微结构可分为拱桥形、棋盘形和筛孔形3 种。拱桥形结构和棋盘形结构比较接近,二者与筛孔形结构差异较明显。在已观察的种类中,线蛱蝶属红线蛱蝶翅鳞片上的纵肋突起最小（200 nm×300 nm）,闪蛱蝶属柳紫闪蛱蝶翅鳞片上的纵肋突起最大（590 nm×560 nm）。鳞片具有相似的形状、结构和排列,尤其是同属蝴蝶翅鳞片超微结构的形状和尺寸差异较小,表明它们之间的亲缘关系接近。