中国东北地区蚱属一新种(直翅目,蚱总科)

报道采自东北地区蚱总科、蚱属昆虫1新种,即长春蚱Tetrix changchunensis sp.nov.;并与其近似种做了比较,模式标本保存于东北师范大学生命科学学院动物标本室.     

口蹄疫病毒P1基因在大肠杆菌中的高效表达及其生物活性的初步分析

将口蹄疫病毒 (FMDV)结构蛋白基因P1的完整cDNA序列插入原核表达性载体pGEX KG中 ,使P1基因与GST融合 ,获得融合表达质粒pKG P1,转化E .coliBL₂₁ (DE3) ,经IPTG诱导 ,SDS PADE结果表明GST P1融合蛋白获得高效表达 ,Western blot检测证实表达的融合蛋白具有免疫学活性 ,表达产物主要存在于细菌裂解液上清中。进一步采用GST纯化试剂盒纯化P1蛋白并作为诊断抗原 ,建立了P1 ELISA诊断方法 ,与FMD间接血凝 (IHA)检测方法平行检测 86 4份血清样品 ,总的符合率达87%。     

TAT蛋白质转导结构域与SV40启动子特异的三锌指结构融合蛋白的表达与纯化

利用蛋白质转导结构域(PTDs)可以将与之融合表达的蛋白质直接送入细胞中。将通过筛选噬菌体展示锌指库得到的特异作用于SV40启动子上9bp序列的三锌指结构的序列插入含有TAT蛋白的蛋白质转导结构域的表达载体pET—TAT-NLS中,构建融合蛋白的表达载体pET-TAT-NLS—clone3。融合蛋白在E．coli BL21(DE3)中得到了可溶性表达,含量约占总蛋白的18％;并通过镍亲和凝胶层析柱得到了较好的纯化融合蛋白。     

哺乳动物细胞高效表达系统研究进展

哺乳动物细胞高效表达系统是基因工程制药研究中的一个重要内容,而表达载体和宿主细胞是哺乳动物细胞表达系统的2个基本组成部分。近年来通过降低目的基因的位置效应、提高目的基因的转录和翻译水平以及目的基因的拷贝数等方面构建哺乳动物细胞高效表达载体。与此同时,研究也对宿主细胞进行了多方面的改造,使之能更好地适应工业化大规模培养的要求,从而降低细胞培养和产品纯化的成本。本就哺乳动物细胞高效表达载体的构建及宿主细胞的改造等方面的最新进展作一简述。     

Various Applications of Soil Contaminant Database Developed for the Central Artery/Tunnel Project

One of the largest environmental assessment programs in the United States was initiated in the early 1990s to determine the chemical characteristics of soil located within the planned alignment for the Central Artery (I-93) / Tunnel (I-90) (CA/T) Project in Boston, Massachusetts. The primary purpose of the program was to support management of the handling and disposal of over 17 million cubic yards of soil to be excavated during construction of the CA/T Project. As part of this work, more than 8,000 soil samples were collected from more than 2,600 soil borings and analyzed for a range of chemical contaminants, including volatile organic compounds, acid/base neutral compounds, total petroleum hydrocarbons, polychlorinated biphenyls, and heavy metals. The soils encountered during the investigations exhibited properties influenced by numerous anthropogenic activities. These activities, such as vehicular emissions, historic industrial/manufacturing operations, and waterfront filling with both building rubble and dredge spoils from Boston Harbor, resulted in soils primarily contaminated with petroleum hydrocarbons and metals. As a result of this program, an extensive database of the chemical constituents present in urban soils in downtown Boston was developed. These results were primarily used to delineate the limits of contaminated areas affecting the planned construction. In addition, the database has been used by the Project to support various soil management activities, as well as by the regulatory community in developing guidelines and criteria governing the management of contaminated soils in Massachusetts. This paper focuses on the various applications of this database throughout the course of the Project, and with the additional aim of stimulating potential future applications by both the regulatory and scientific communities.     

Comparative Analysis of Eubacterial DNA Polymerase III Alpha Subunits

DNA polymerase III is one of the five eubacterial DNA polymerases that is re-sponsible for the replication of DNA duplex. Among the ten subunits of the DNApolymerase III core enzyme, the alpha subunit catalyzes the reaction for polymer-izing both DNA strands. In this study, we extracted genomic sequences of thealpha subunit from 159 sequenced eubacterial genomes, and carried out sequence-based phylogenetic and structural analyses. We found that all eubacterial genomeshave one or more alpha subunits, which form either homodimers or heterodimers.Phylogenetic and domain structural analyses as well as copy number variations ofthe alpha subunit in each bacterium indicate the classification of alpha subunit intofour basic groups: polC, dnaE1, dnaE2, and dnaE3. This classification is of essencein genome composition analysis. We also consolidated the naming convention toavoid further confusion in gene annotations.     

低温对水稻类囊体膜蛋白磷酸化及光合机构光能分配的影响

研究了低温胁迫对水稻类囊体膜蛋白磷酸化和光能分配的影响。类囊体膜蛋白组分的SDS-PAGE和免疫印迹分析结果显示,低温弱光条件下光系统Ⅱ(PSⅡ)功能蛋白的稳态水平均有所降低。低温(77K)荧光分析表明,低温处理后类囊体膜光能吸收明显下降,而且FPSⅡ/FPSⅠ的比值均较对照组下降,表明低温弱光条件下有更多的激发能被分配到PSⅠ。低温处理同时还改变了类囊体膜蛋白磷酸化水平,捕光天线LHCⅡ蛋白中lhcb1的磷酸化水平明显降低,lhcb2的磷酸化水平增加,进一步证实lhcb2向PSⅠ移动,改变光能分配。PSⅡ反应中心D1、D2蛋白和核心天线CP43的磷酸化水平增高,有利于PSⅡ二聚体的稳定。     

农杆菌抑菌剂的抑菌效果及其对小黑杨叶片不定芽产生率的影响

研究了几种抗生素及AgNO3对农杆菌的抑菌效果和对小黑杨花粉植株叶片不定芽产生率的影响.确定首选抑菌抗生素是头孢噻肟钠,AgNO3的抑菌效果也很好,两者的有效抑菌浓度分别为200和3.0 mg·L-1,抑菌时间均为9 d,此浓度下小黑杨叶片的不定芽产生率为88%和89%.     

低温贮藏期间百合鳞茎中的游离氨基酸组分和含量变化

百合鳞茎的游离氨基酸主要集中在顶芽和内部鳞片等幼嫩组织中,含量最高、变化最大的是精氨酸.谷氨酸族的氨基酸在鳞茎代谢中起重要作用.顶芽的氨基酸总量及精氨酸含量随贮藏温度的升高而增加.贮藏过程中,鳞茎盘的氨基酸含量下降,顶芽和贮备组织中其含量有明显升高的过程.贮藏前期34 d内游离氨基酸含量发生显著变化.