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71.
Jie SHEN * Dong Bao YU * Chinese Center for Disease Control Prevention China Division of Policy Study Information National Center for AIDS Prevention Control China 《Cell research》2005,(Z1)
EVOLUTION OF THE GOVERNMENT POLI- CIES Looking back 20 and more years, one could have hardly imagined that such great changes would have happened to the governmental policies on HIV/AIDS in China. In the earlier phase of the epidemic, laws and regulations were drafted based on the practices and experiences of preven- tion and control of other infectious diseases, such as isolation, quarantine and compulsory testing and examina- tions etc. When the HIV/AIDS epidemic was firstl… 相似文献
72.
等位基因特异性引物PCR技术及其应用研究 总被引:15,自引:0,他引:15
目的:研究建立等位基因特异性引物PCR技术体系,并将其应用于基因单核苷酸多态性研究工作。方法:通过美国国家生物信息中心(NCBI)的genBank获取基因序列及其相应位点的SNP信息。利用Primer5.0软件设计引物,并经NCBI的Blast2.0软件检验其特异性。结果:建立了单一等位基因特异性引物PCR(SASP—PCR)与嵌套式等位基因特异性引物PCR(NASP-PCR)两种技术,并应用于β2肾上腺素受体及内皮源性一氧化氮合酶基因单核苷酸多态性的研究,证实该技术的稳定性和优越性。结论:等位基因特异性引物PCR技术是一种更为简便、特异性较高、费用少的、便于推广的SNP检测方法,特别是在群体基因单核苷酸多态性研究中更有优势。 相似文献
73.
Yang Ping He Yu-quan Zeng Hong Ni Jin-song Yun Qing-jun Huang Xiao-ping Li Shu-mei .Cadiovascular Department China-Japan Union Hospital of Jilin University Changchun P. R. China . Department of Pathdogy School of Basic Medical Sciences of Jilin University Changchun P. R. China . Cardiovascular Department Second Hospital of Jilin University Changchun P. R. China 《仿生工程学报(英文版)》2005,2(2):87-91
1Introduction Congestiveheartfailureisamultipleaetiology,high prevalence,cardiovasculardisorderwithpoorprognosis. Medicaltreatmentofdilatedcardiomyopathyisaimedat alleviatingthesymptomsofheartfailure.Diuretics,ACE inhibitorsandbeta blockershavefavourableeffectson symptoms,exercisecapacityandmortality[1-3].Growth hormone(GH)andinsulin likegrowthfactor(IGF) 1 areinvolvedinseveralphysiologicalprocessessuchas thecontrolofmusclemassandfunction,bodycomposi tionandtheregulationofnutrientmetaboli… 相似文献
74.
Shi Wei-ping Ren Lu-quan Tian Li-mei .The Key Lab of Terrain-Machinery Bionic Engineering 《仿生工程学报(英文版)》2005,(3)
1IntroductionAdhesive forces exist between soil and the surfacesof soil-engaging components[1,2].Soil adhesion increasesthe running resistance and energy consumption,andaffects the operating quality.Soil adhesion also reducesthe working productivity of terrain machines,even worseit makes terrain machines fail to run.Reducing theadhesive force of the soil-engaging machines will have aprofound influence for cultivation.Through theinvestigation of soil animals,we have found that soilanimals poss… 相似文献
75.
我国北方植被指数对土壤湿度的敏感性分析 总被引:7,自引:0,他引:7
土壤湿度对植被指数起着重要的作用。利用NOAA-AVHRR数据中的植被指数(NDVI)和我国气象台站所监测的土壤湿度数据,对我国北方地区的NDVI与土壤湿度的关系进行了研究。结果表明,近18年来,北方地区土壤湿度不同区域其变化趋势存在差异,西北地区土壤湿度在增加,而华北和东北地区土壤湿度在下降。NDVI对土壤湿度的响应也存在着明显的区域差异,我国西北地区植被对土壤湿度的敏感性最强,其次是华北地区,敏感性弱的是东北地区;对于不同层次的土壤湿度,也表现为同样的区域特点。但随着土壤深度的增加,敏感性减弱。这种敏感性也表现在不同季节变化上,春季华北地区土壤湿度对植被指数影响较大,其次是西北和东北地区,夏季和秋季北方地区土壤湿度与植被指数都有较好的相关性,以西北地区的相关系数最大,而冬季北方地区植被指数对土壤湿度都不敏感。 相似文献
76.
Li YANG Jiang CHEN Catherine C. Y. CHANG Xin-Ying YANG Zhen-Zhen WANG Ta-Yuan CHANG and Bo-Liang LI* State Key Laboratory of Molecular Biology Institute of Biochemistry Cell Biology Shanghai Institutes for Biological Sciences the Chinese Academy of Sciences Shanghai China Department of Biochemistry Dartmouth Medical School Hanover NH USA 《Acta biochimica et biophysica Sinica》2004,(4)
Acyl-coenzyme A:cholesterol acyltransferase (ACAT)is an integral membrane protein, which is mainly locatedin rough endoplasmic reticulum (ER), and is responsiblefor catalyzing the intracellular formation of cholesterylester from cholesterol and long-chain fatty acyl-coenzymeA [1,2]. Human ACAT1 cDNA K1 was firstly cloned andfunctionally expressed in 1993 [3]. Further studies withspecific anti-ACAT1 antibody (DM10) illustrated that onemajor 50 kD ACAT1 protein was expressed in various… 相似文献
77.
ZHENG Junfang LIU Guirong ZHU Wanfu ZHOU Yuguang & LIU Shulin . Department of Microbiology School of Basic Medical Sciences Peking University Beijing China . Department of Microbiology Infectious Diseases University of Calgary Calgary ABTN N Canada . Institute of Microbiology Chinese Academy of Sciences Beijing China 《中国科学:生命科学英文版》2004,47(3):268-278
Rhizobiaareagriculturallyandenvironmentallyimportantbacteria.Theirsymbiosiswithleguminousplantsisresponsibleformostoftheatmosphericni-trogenfixedonland.Classificationofthesebacteriabasedontheirnaturalrelationshipswillpromotetheirapplication.Thisresearchemploysanewphylogeneticmethod,i.e.,revelationandcomparisonofgenomestructure,tocategorizerhizobia.Phylogenyisthestudyoftheevolutionaryrelationshipsamongorgan-isms[1].Currentlyphylogeneticrelationshipsamongrhizobiaaremostlyinferredfromcomparisons… 相似文献
78.
Differentiation potential of bone marrow mesenchymal stem cells into retina in normal and laser-injured rat eye 总被引:5,自引:0,他引:5
ZHANG Jie SHAN Qing MA Ping JIANG Yanming CHEN Peng WEN Jingxia ZHOU You QIAN Huanwen & PEI Xuetao . Beijing Institute of Radiation Medicine Beijing China . Beijing Institute of Transfusion Medicine Beijing China 《中国科学:生命科学英文版》2004,47(3):241-250
Withinthebonemarrowstromathereexistsasubsetofnonhematopoieticcellsreferredtoasmes-enchymalstemormesenchymalprogenitorcells.Mesenchymalstemcells(MSCs)areapopulationofpluripotentcellswithinthehuman,birdorrodentbonemarrowmicroenvironmentdefinedbytheirability,eitherinvitroorinvivo,todifferentiateintocellsoftheosteogenic,chondrogenic,tendonogenic,adipo-genic,neuralcellsandmyogeniclineages[1].Themethodologiestoisolateandculture-expandMSCsfromhumanbonemarrowforestablishingthecellularortissuediffere… 相似文献
79.
CHENG Lingpeng CHEN Senxiong Jenifer M. Brannan Joanita Jakana ZHANG Qinfen Z. H. Zhou & ZHANG Jingqiang . State Key Laboratory for Bio-control Zhongshan University Guangzhou China . Department of Pathology Medicine University of Texas-Huston Medical School Huston TX USA . National Center for Macromolecular Imaging Baylor College of Medicine Houston TX USA 《中国科学:生命科学英文版》2004,47(3):224-228
Apreviously-unknownviruswasfoundchroni-callyinfectingC6/36cellsthatwereusedtoculturedenguevirus.Afterisolationandpurification,RT,PCRandsequencingwereperformedwithrandomprimers.TheresultsshowthattheviralgenomeisssDNAandis4096ntinlength(GenBankAccessionNo.AY095351).Thenucleicacidsequenceofthispreviously-undescribedvirussharesabout90%iden-titywiththatofAedesaegyptidensonucleosisvirusandtheaminoacidsequencesofthreeproteinsen-codedbythenucleicacidshareabout89%—93%identitieswiththoseofAedesa… 相似文献
80.
ZHOU Jie'''' ZHAO Ruqian WEI Xihui XIA Dong XU Qingfu & CHEN Jie . Key Laboratory of Animal Physiology Biochemistry Ministry of Agriculture Nanjing Agricultural University Nanjing China . Anhui Agricultural University Hefei China 《中国科学:生命科学英文版》2004,47(2):190-196
~~Developmental patterns of serum leptin levels, leptin gene expression in adipose tissue and Ob-Rb gene expression in hypothalamus of Erhualian and Large White pigs~~ 相似文献