油菜花序轴、叶片组织培养中形态发生的细胞组织学研究

油菜花序轴、叶片在MS培养基上经诱导可产生愈伤组织并形成再生植株。愈伤组织形成可分3个时期:细胞启动期、分裂期和分化期。实验证明:花序轴启动部位多发生在皮层薄壁细胞、髓部薄壁细胞、韧皮薄壁细胞和木薄壁细胞。叶片主要是叶肉细胞、叶脉薄壁细胞和表皮细胞启动。细胞分裂期的特点是启动细胞反复增殖,形成连续的分生细胞层。细胞分化期的特点是一部分细胞保持分裂能力,一部分细胞分化形成薄壁细胞和输导分子。观察了愈伤组织中器官发生的部位和方式。实验证明,根的发生多为内起源,芽的发生多为外起源。     

酸雨对翅荚木的生理反应及其机理的研究

本文指出了酸雨对翅荚木细胞透性、pH值、等电点、耐酸力均有显著的影响。其影响是随酸雨pH值的下降和浸没时间的延长而增加。发芽种子的抗坏血酸氧化酶、多酚氧化酶的活性,呼吸强度和可溶性糖、粗脂肪和蛋白质的含量,也是随酸雨pH值的下降而降低。叶绿素总量、叶绿素a,b和a/b的比值,pH 5处理与对照无差异,低于pH4以下的处理叶绿素a含量则大幅度减少。叶绿素b含量则较稳定。根系的活力也是随酸雨pH值下降而急剧减少。酸雨对翅荚木种子的发芽率、出苗率及胚和幼苗的生长,都表现有不同程度的抑制效应,酸雨对叶片的伤害,也是随酸雨pH值的降低、喷洒次数的增多和浸没时间的延长而加重。     

猪肺炎支原体膜上ATP酶生化性质的研究

猪肺炎支原体膜上ATP酶为Mg~(2+)激活,乌巴因不抑制。DCCD和寡霉素对该酶也无抑制作用,只有NBD与Quercetin才有一定的抑制效果。用梯度凝胶电泳可获均一的具有活性的酶蛋白带。     

尾壳核内注射脑啡肽和bestatin对大鼠操作式条件...

Female Wistar rats were trained in a Skinner-box, 30 trials per day in a dark room to establish operant defence conditioning. Training started with a light (15 s), then combined with footshock for further 8 s. When the rats learned to press the key to avoid footshock within 15 s, conditioned response was considered established. After the rats reached a conditioning rate (CR) above 80% for 5 days, cannulae were implanted into caudate-putamen. Two to three days later, Met-enkephalin (MEK) or bestatin (an aminopeptidase inhibitor) was injected bilaterally into caudate-putamen. 30 min, 2 h, 24 h and 48 h after injection, conditioning tests were conducted, with each session consisting of 30 trials. Control experiments were done when 0.9% NaCl (NS) was injected. After injection of NS, CR maintained above 80% in all 4 test sessions. MEK (60 ng/rat) or bestatin (10 micrograms/rat) significantly lowered the CR during the 30 min and 2 h test session. In the latter case, the latency (L) was also prolonged. However both CR and L returned to the control level in the 24 h and 48 h test sessions. Naloxone (2 mg/kg, i.p.) blocked the conditioning-depression effect of bestatin. No significant alteration was seen in locomotor activity after MEK or bestatin injection. The results suggest that enkephalin in caudate-putamen may be involved in the regulation of retrieval of conditioning. Bestatin mimics the effect of MEK on conditioning reflex probably by increasing production of endogenous enkephalin.     

PP333对油菜种间杂种试管苗生长发育的影响

甘蓝型油菜(Brassica napus)品种Willi与埃塞俄比亚芥(B.carinata)F_4代种子,白菜型油菜(B.campestris)品种郫县早黄油菜和七星剑作母本与埃塞俄比亚芥杂交授粉后胚发育到手杖期的不饱满种子,用75％乙醇处理30s,再用0.2％HgCl_2浸泡12min,无菌水冲洗3次。甘蓝型作母本的组合接种在1/2MS大量元素和1/2MS附加不同浓度的PP333的液体和固体等7种培养基上。     

HPLC法定量分析植物组织中ABA,IAA和NAA

近年来有关植物激素的提取、分离方法的报道时有出现,但大都是沿用80％甲醇(MeOH)从植物组织中提取IAA和ABA,用乙酸乙酯萃取酸化的提取液后得到粗提液,粗提液再经过纤维素薄层层析、硅胶层析、     

中国农业害虫生物防治概况与进展

<正> 随着我国经济建设的不断发展和社会对生态环境意识的增强,生物防治科技工作在政府大力支持和广大科技人员共同努力下,得到迅速发展。“七五”和“八五”期间经政府部门批准正式列入国家科技攻关计划,并由中国农科院生防所主持,组织全国30多个科研和教学单位4百多位科技人员进行协作攻关。与此同