Proteome analysis of hepatic non-parenchymal cells of immune liver fibrosis rats

Elucidation of the mechanisms of liver fibrogenesis is important to treat liver fibrosis.In this study,we established rat models of liver fibrosis with stages from 0–1,2,and 3–4 to 4 at 2,4,6,and 8 weeks,respectively,by injection of pig serum.Liver fibrogenesis was detected by Masson’s trichrome staining.Rat non-parenchymal cells(NPCs)were enriched 4-fold by Percoll density gradient centrifugation.Protein extracts from NPCs were prepared at 4 and 8 weeks,separated by two-dimensional electrophoresis,and then stained with Coomassie Blue G-250.At 4 weeks,we identified 18 non-redundant differentially expressed proteins of which protein disulfide-isomerase associated protein 3(PDIA3)and NDUV showed consistent expression at protein and mRNA levels from 4 to 8 weeks.PDIA3 was found to be down-regulated by Western blotting in the rat model and immunohistochemically in human liver.Our results revealed important aspects of the pathogenesis/progression of liver fibrosis and demonstrated important changes in protein expression levels of NPCs at various stages of liver fibrosis.     

ABA和SA对于提高植物抗旱及抗盐性的研究进展

植物受到环境胁迫后体内会产生活性氧自由基等有害物质,破坏质膜透性,导致植物生长受到抑制。经研究发现脱落酸(ABA)和水杨酸(SA)作为植物的生长调节物质对于提高植物抗性,维持植物正常生长具有重要的意义。综述近年来国内外有关ABA和SA提高植物抗性的最新进展,为研究提高植物抗性提供理论参考。     

Atg5 regulates late endosome and lysosome biogenesis

Autophagy is an evolutionarily conserved lysosome-based degradation process.Atg5 plays a very important role in autophagosome formation.Here we show that Atg5 is required for biogenesis of late endosomes and lysosomes in an autophagy-independent manner.In Atg5 cells,but not in other essential autophagy genes defecting cells,recycling and retrieval of late endosomal components from hybrid organelles are impaired,causing persistent hybrid organelles and defective formation of late endosomes and lysosomes.Defective retrieval of late endosomal components from hybrid organelles resulting from impaired recruitment of a component of V1-ATPase to acidic organelles blocks the pH-dependent retrieval of late endosomal components from hybrid organelles.Lowering the intracellular pH restores late endosome/lysosome biogenesis in Atg5 cells.Our data demonstrate an unexpected role of Atg5 and shed new light on late endosome and lysosome biogenesis.     

烟叶果胶质分解菌的选育

从烟叶叶面分离到产果胶酶真菌 18株 ,在此基础上 ,进行液体培养 ,测定酶活 ,得到一株酶活性较高的黄曲霉DPE - 0 0 5。对该菌株产酶培养基的碳、氮源进行正交法研究 ,正交实验的结果表明 ,影响该菌产酶活性的因素依次为A(麸皮 ) >B(乳糖 ) >C(果胶 ) >D(硫酸铵 ) ,其最佳组合为A2B3C3D1。最适产酶条件为 :麸皮 4 0 % ,果胶 0 3% ,乳糖 1 5 % ,(NH4 ) 2 SO4 0 5 % ,KH2 PO4 0 2 5 % ,MgSO4 ·7H2 O 0 0 5 % ,NaNO30 0 2 % ,FeSO4 ·7H2 O 0 0 0 1% ,起始pH 6 0 ,在 2 8℃摇床培养 7d产酶量达到最高。以玉溪B3F烟叶为材料 ,施加DPE - 0 0 5菌株所产酶液 ,在 5 0℃贮存 12h。经化学成分检测 ,结果表明 ,果胶质降低了 18 15 %。     

地霉属真菌和棒状杆菌属菌株协同发酵生产γ-癸内酯

使用有油脂水解活性的地霉属菌株PL-4与有内酯合成能力的棒状杆菌属菌株RS105进行协同发酵转化蓖麻油生产γ-癸内酯,发酵产物用GC/MS（气质联用）方法进行检测,结果显示使用具有油脂降解能力的地震属真菌PL-4协同发酵较单独使用RS105发酵时内酯的产率有明显提高。     

去叶对不同生长习性大豆固氮作用的影响

生殖生长期开始去叶后 ,(1)有限型、亚有限型和无限型 3种类型的大豆根瘤固氮酶活性都降低 ,而根瘤中酰脲含量则不同程度地增加 ;(2 )有限型大豆幼茎中酰脲含量明显增加 ,但亚有限型和无限型大豆变化不大 ;(3) 3种类型大豆幼茎中硝态氮含量增加明显     

利用基因芯片对一高保守基因进行肿瘤相关分析

本文对大规模人类cDNA 测序过程中获得的一条高保守基因进行了初步功能研究,生物信息学研究发现该基因在人类、小鼠、果蝇、拟南芥和裂殖酵母中都有很高的保守性,其他分析预测该基因可能具有肿瘤相关性。RT-PCR分析表明,该基因在成人和胎儿组织中广谱表达。利用基因芯片分析该基因在7例肝癌、5例胰腺癌、2例喉癌和2例肺癌中表达情况,结果证实了该基因的肿瘤相关性,并且提示该基因在不同肿瘤类型中可能处于不同的地位。 Abstract:Preliminary function research of a highly conserved human gene,which was cloned from human fetal cDNA library during large-scale cDNA sequencing,is illustrated in this article.Bioinformatics analysis indicates that this gene is highly conserved in human、mouse、fruit fly、thaliana and fission yeast.Other bioinformatics analysis implies its relevance with tumors.RT-PCR analysis shows its wide-ranging expression patterns.Its expression in 16 cancer cases(including 7 liver cancer cases、5 pancreas cancer cases、2 larynx cancer cases and 2 lung cancer cases) is studied by using gene microarray analysis.The result shows its relevance with tumors and implies it may have different status in different classification of tumors.     

广东玉米区域试验新组合的模糊综合评价

应用模糊数学中的模糊综合评价方法对广东省1999—2001 年春植玉米区域试验新组合进行定量化评价。结果表明,粤单2号、南玉 18号与南玉35号分别居于1999—2001年区试组合评价第一位;参试两年的组合粤单2号、南玉18号、登海3号与华引115号综合评价较高,表现稳定,可以在广东省内推广应用。 Abstract:The fuzzy comprehensive evaluation method was applied to the quantitative evaluation of the maize new cross combinations from 1999 to 2001 in Guangdong province.The results showed that Yuedan 2,Nanyu 18 and Nanyu 35 ranked the first among the combination evaluations of regional trials in 1999-2000.The comprehensive evaluation values of the combination of Yuedan 2,Nanyu 18 and Denghai 3 were high and stable in the successive two-year experiment.So they were recommended to popularize in Guangdong province.     

Phylogeny of Ptychostomum （Bryaceae,Musci） inferred from sequences of nuclear ribosomal DNA internal transcribed spacer （ITS） and chloroplast rps4

The phylogeny of Ptychostomum was first spacer （ITS） region of the nuclear ribosomal （nr） DNA DNA rps4 sequences. Maximum parsimony, maximum undertaken based on analysis of the internal transcribed and by combining data from nrDNA ITS and chloroplast likelihood, and Bayesian analyses all support the conclusion that the reinstated genus Ptychostomum is not monophyletic. Ptychostomum funkii （Schwagr.） J. R. Spence （≡ Bryum funkii Schwaigr.） is placed within a clade containing the type species of Bryum, B. argenteum Hedw. The remaining members of Ptychostomum investigated in the present study constitute another well-supported clade. The results are congruent with previous molecular analyses. On the basis of phylogenetic evidence, we agree with transferring B. amblyodon Mull. Hal. （≡ B. inclinatum （Brid.） Turton≡ Bryum archangelicum Bruch ＆ Schimp.）, Bryum lonchocaulon Mull. Hal., Bryum pallescens Schleich. ex Schwaigr., and Bryum pallens Sw. to Ptychostomum.