宏基因组学在胃肠道微生物研究中的应用

动物胃肠道中普遍存在大量共生微生物群,对于它们的研究一直受制于纯培养技术。随着分子生物学的快速发展及其在微生物学及生态学上的应用,针对未培养微生物研究的一门新型学科——宏基因组技术应运而生并迅速发展。通过提取胃肠道粘膜表面以及内容物中微生物DNA,构建总DNA文库的方法,利用基因组学的研究策略,来研究胃肠道中微生物遗传组成及群落功能。宏基因组技术在胃肠道微生物研究中广泛的应用,对于医学、生态学、生物能源利用等领域的研究具有重大的价值。     

牧民对高寒牧区生态环境的感知——以甘南牧区为例

地处青藏高原的甘南牧区是典型的生态敏感区,牧民作为牧区最主要的经济活动主体与最基本的决策单位,其对生态环境的认识和响应影响着牧区的生态安全.采用参与性农户评估方法(PRA)对甘南牧区的97户牧民家庭进行了调查,并根据分布区域将其分成纯牧区牧户和半农半牧区牧户两组,就牧民的环境意识、对环境变化的感知、对环境变化原因的认识进行比较分析,基于分析结果,提出了相关的政策建议.研究结果表明:研究区牧民对生态环境重要性的认识非常明确,但在生产中却较少真正考虑对生态环境的影响;与20a前相比,85%的牧户认为生态环境有所恶化,纯牧区牧户的感受更为深刻;对于生态环境恶化的原因,超过40%的牧户认为是超载过牧,其次是破坏植被(22.68%)和气候变化(18.56%).两组牧户对于生态环境恶化原因的认识具有一定差别,纯牧区牧户认为主要原因是过度放牧及气候变化,半农半牧区牧户认为是过度放牧与破坏植被、滥垦土地.     

海南植物区系的多样性

记载海南维管束植物的多样性,表明蕨类植物区系已有古生代和中生代的孑遗,又有白垩纪以来的现代蕨类,它们都存在着许多特有种;裸子植物则以泛热带成分及华夏成分为主;有花植物包括全球植物区系8个植物区的成分,而以热带成分及全球性分布成分最多。最后,文章分析了海南植物区系多样性的地史背景及自然条件的因素。     

海南植物区系的多样性

记载海南维管束植物的多样性,表明蕨类植物区系已有古生代和中生代的孑遗,又有白垩纪以来的现代蕨类,它们都存在着许多特有种;裸子植物则以泛热带成分及华夏成分为主;有花植物包括全球植物区系8个植物区的成分,而以热带成分全球性分布成分最多。最后,文章分析了海南植物区系多样性的地史背景及自然条件的因素。     

微生物课程改革与职业技能鉴定衔接初探

高职院校开展职业技能鉴定,推行职业资格证书制度,是落实“科教兴国”战略方针的重要举措。推行“双证书”制度,创新人才培养模式,强化学生技能训练,使学生在获得学历证书的同时,顺利获得相应的职业资格证书,是高职教育的发展方向。本文根据职业技能鉴定工作与微生物教学改革衔接过程中的具体实践,提出了这方面工作的途径和见解。     

多异瓢虫的发育与温度的关系

测定多异瓢虫Hippodamia variegata(Goeze)在13,17,21,25,29和33℃下各虫态的发育历期。结果表明,在不同温度梯度下,多异瓢虫全世代历期依次为:(62.23±3.42)d,(48.31±2.96)d,(25.01±2.53)d,(20.78±1.83)d,(13.68±0.67)d和(10.92±0.58)d。利用最小二乘法确定出多异瓢虫各虫态的发育起点温度和有效积温,其全世代的分别为10.85℃和249.79日.度;由微分法求出Logistic曲线方程中的最大发育速率K,进而由最小二乘法求出a、r,从而确定出多异瓢虫各虫态的发育速率与温度的Logistic曲线关系,其全世代的为:V=0.101156/[1+exp(3.905041-0.183509T)];由Lagrangc中值定理求出各虫态的发育最适温度、适宜温区,其全世代的分别为:Tmid(最适温度)=21.28℃、Tmin(适宜温区下限)=11.55℃、Tmax(适宜温区上限)=31.01℃。     

Jurisdiction Over Underwater Cultural Heritage in the EEZ and on the Continental Shelf: A Perspective From the Practice of States Bordering the South China Sea

Abstract

The Chinese initiative of constructing the 21st Century Maritime Silk Road could be identified as a new chance to promote the protection of underwater cultural heritage (UCH) in the South China Sea. However, uncertainties concerning the jurisdictional issue over the UCH in the exclusive economic zone (EEZ) or on the continental shelf constitute an obstacle. The Convention on the Protection of Underwater Cultural Heritage has, to some extent, enlarged the coastal state’s jurisdiction. State practice differs on this issue. This article focuses on the domestic legislations of states bordering the South China Sea related to the jurisdiction over UCH found in their EEZ or on their continental shelf.     

长江三峡淹没区与移民安置区植物多样性及其保护策略

随着三峡大坝的修建,三峡地区淹没区和移民安置区的植物多样性调查与保护工作相继展开。从多年考察采集到的标本及历史资料补充确定,两区高等植物为170科,762属,1784种。分别占到三峡地区高等植物科、属、种数的85.85%,75.30%和59.19%。其中特有植物27种。而三峡地区的灌木和草丛群落基本分布在沿江两岸的低海拔地区,受水库蓄水影响较大。对两区内21个马尾松(Pinus massoniana)群落物种多样性进行的Shannon-Wiener指数及Pielou均匀度指数的测定结果表明,马尾松群落的多样性变化总体趋势为:灌木层>草本层>乔木层;对该地区具有代表性的11种灌丛类型进行的物种多样性指数的测定结果表明:盐肤木(Rhus chinensis)、毛黄栌(Cotinus coggygriavar.pubescens)、荆条(Vitex negundo)、马桑(Coriaria sinic)等4种群落的灌木层丰富度较高,分别为16,26,20和15。多样性指数分别为1.791,3.427,2.949和1.718;对沿江分布的9种主要草丛群落进行的物种多样性指数的测定结果表明:芒萁(Dicranopteris dichotoma)、五节芒(Miscanthus floridutus)、白茅(Imperatacylindricavar.major)、荩草(Arthraxon hispidus)等4种海拔分布较高的群落物种多样性指数较高,分别达到1.697,1.354,1.144和1.018。另外,对淹没区及移民安置区的物种调查结果显示:受淹的自然植被类型共有22种。其中木本群落4种,灌丛9种,草丛9种。小鞍叶羊蹄甲(Bauhinia brachycarpavar.microphylla)、中华蚊母树(Distylium chinensis)、水杨梅(Geum aleppicum)、小叶黄杨(Buxus sinica var.parvifolia)、铁仔(Myrsine africana)、疏花水柏枝(Myricaria laxiflora)等灌丛被全部淹没;巫溪叶底珠(Securinega wuxiensis)、宜昌黄杨(Buxus ichangensis)和荷叶铁线蕨(Adiantum reniformevar.sinense)大部分被淹没。目前,已建立了库区植物物种保护站及监测站,200多种植物已得到迁地保护,包括已列入中国植物红皮书的37种珍濒物种和11种三峡库区建群种。     

微生物课程改革与职业技能鉴定衔接初探

高职院校开展职业技能鉴定,推行职业资格证书制度,是落实“科教兴国”战略方针的重要举措。推行“双证书”制度,创新人才培养模式,强化学生技能训练,使学生在获得学历证书的同时,顺利获得相应的职业资格证书,是高职教育的发展方向。本文根据职业技能鉴定工作与微生物教学改革衔接过程中的具体实践,提出了这方面工作的途径和见解。     

植物种群分子进化中对生境的适应——对荒漠植物遗传多样性研究结果的初步分析

长期以来,自然选择理论与中性理论对生物分子进化中的环境适应机理存在着激烈争论。目前,在植物种群分子进化中对生境适应的研究中正面临着一些难题：中性突变是分子水平进化的唯一原因,自然选择发挥主要作用的适应性进化是否存在于分子水平,选择与中性两种学说两种机制完全不同,如何才能将两者联系和统一起来,部分学者利用建立各种模型来描述自然选择对分子标记位点以及连锁序列的直接作用,如生态位宽度变异假设等。本研究小组以新疆阜康荒漠植物为研究对象,通过对两种重要荒漠植物遗传多样性的研究,分析两种植物各亚种群不同生境的生态因子与其遗传变异的关系,讨论生态位宽度变异假设,揭示遗传变异的产生与维持。中性论者与选择论者都试图解释生物环境适应与分子变异之间的关系。中性论和选择论是反映进化的两个侧面,它们不是绝对的,可以相互转化。     

天气变化对人口死亡率的影响——以广州市和上海市为例

人口死亡率与天气有一定的关系,以广州、上海两市１０个冬季和夏季的逐日死亡资料和气象资料,利用统计学和天气学方法得出,在最高温度达３４℃的“热日”,各类死亡数明显增多;而冬季死亡数随最低温度的下降而略有增加;广州夏季最大死亡率出现在“阴凉型”天气,而上海则是在“晴热型”天气,且上海死亡率受最高温度的影响大于广州;广州和上海冬季死亡率最大的天气均是“严寒型”,但广州死亡率受低温的影响要比上海大．在其他气象要素相似的情况下,风速对死亡率有一定的影响．     

Then and Now: Reappraising Freedom of the Seas in Modern Law of the Sea

The principle of freedom of the seas remains the governing paradigm of the high seas in modern law of the sea. Although the principle, as embraced by the UN Convention on the Law of the Sea (LOSC), is no longer an absolute norm, it continues to present fundamental challenges for achieving effective conservation on the high seas as it stands in direct contrast to the conservation duty imposed on states by LOSC. The recent UN General Assembly resolution calling for the adoption of a further Implementing Agreement under LOSC to address conservation on the high seas, highlights the need to build a new ethos for management of the high seas, which will require states to loosen their firm grip on the Grotian doctrine. This article seeks to contribute toward shifting attitudes in relation to the principle of freedom through an examination of the nature and scope of the principle in its historic context and in contemporary law of the sea.     

黑河中下游绿洲生态安全评价

生态安全包括自然生态安全、经济生态安全和社会生态安全 ,尤指自然和半自然生态系统的安全 ,即生态系统完整性和健康的整体水平反映。生态安全是维护地区或国家乃至全球的生态环境不受威胁的状态 ,能为整个生态经济系统的持续发展提供生态保障。从水安全、土地安全、经济社会安全出发 ,选取水资源量供需比、地表水质量、地下水埋深和地下水矿化度 4种水安全评价指标 ;土地人口承载度、林地覆盖率、草地载畜度、绿洲面积变化率、绿洲土地沙化率、绿洲土地盐碱化率和抗灾度等 7种土地安全评价指标 ;人均 GDP产值、单位面积农业生产总值、农民人均纯收入、绿洲人口密度、城市化率和文明度等经济社会安全评价指标 ;共计 17项 ,建立了绿洲生态安全评价综合指标体系。然后 ,应用综合评价法、层次分析法和 GIS等多种方法与手段 ,在绿洲尺度上对黑河干流中下游地区的张掖、临泽、高台、鼎新和额济纳 5个绿洲进行绿洲生态安全综合评价。评价结果显示 :自黑河中游至下游 ,各个绿洲的生态安全状况依次降低 ,最下游的额济纳绿洲生态安全仅为 4级的差状态。其中 ,水安全是关键 ,土地安全是载体 ,经济社会安全是保障 ,以上几方面相互关联 ,共同作用导致绿洲的生态安全综合水平。     

Third Party Intervention in the Commission on the Limits of the Continental Shelf Regarding a Submission Involving a Dispute

The Commission on the Limits of the Continental Shelf (CLCS) was established in accordance with Article 76 of the Law of the Sea Convention. CLCS has the mandate to consider data submitted by coastal states concerning the outer limits of their continental shelf in areas where those limits extend beyond 200 nautical miles and to make recommendations to the submitting state on matters related to the establishment of the limits. For third parties that have actual or potential disputes, unresolved borders, or unresolved land or maritime disputes with the submitting state, the recommendations of the CLCS can be very sensitive. The focus of this article is on the practice of third parties in responding to submissions and how third parties have constructed their Notifications.     

Today's Customary International Law of the Sea

International courts and tribunals, governments, and scholars over the past half-century (many in the past two decades) have identified various provisions of the 1958 and 1982 treaties on the law of the sea that are customary international law and thus binding on all states, including those not party to these treaties. This article systematically collects these opinions and identifies provisions that have not yet attracted their attention.     

A primer of community ecology using the R language

Community ecology beginners often struggle to understand theories expressed in complex mathematical formulas and to master computer programming. To remedy this situation, this article provides a practical, R-based introduction to community ecology by illustrating core concepts (vital rates, carrying capacity, and density dependence) and models that can be used to explore the patterns of species abundance and diversity. The structure of this article consists of three modeling exercises, each asking a general question that can be answered by a combination of theory and R programming: (1) what determines the abundance of species, and what makes a population persist and go extinct?; (2) what determines the distribution of species and species diversity?; (3) what determines the relative abundance of species and what allows species to coexist? Through the exercises, I discuss the following five concepts and ideas that provide valuable insights into the questions: (i) the tragedy of the commons, (ii) the theory of island biogeography, (iii) competitive exclusion, (iv) the neutral theory of biodiversity, and (v) frequency dependence. These materials are thus designed to guide the reader in developing an intuition for ecological thinking that will help capture the essence of the global environmental and biodiversity crisis. Although this article does not delineate the scope and depth of the vast field of community ecology, I hope that it will motivate the reader to step up to a more formal introduction to community ecology.     

The key role of the energized state of Saccharomyces cerevisiae mitochondria in modulations of the outer membrane channels by the intermembrane space proteins

Mitochondria of the yeast Saccharomyces cerevisiae constitute a perfect model to study the outer membrane channel modulation as besides the TOM complex channel they contain only a single isoform of the VDAC channel and it is possible to obtain viable mutants devoid of the channel. Here, we report that the fraction of the intermembrane space isolated from wild type and the VDAC channel-depleted yeast mitochondria, except of the well-known VDAC channel modulator activity, displays also the TOM complex channel modulating activity as measured in the reconstituted system and with intact mitochondria. The important factor influencing the action of both modulating activities is the energized state of mitochondria. Moreover, the presence of the VDAC channel itself seems to be crucial to properties of the intermembrane space protein (s) able to modulate the outer membrane channels because in the case of intact mitochondria quantitative differences are observed between modulating capabilities of the fractions isolated from wild type and mutant mitochondria.     

相地合宜·借景随机—基于场地特征的乡村景观设计探索

针对当前乡村景观设计“千村一面”的现象进行剖析,反思美丽乡村建设的困惑。提出传统造园中的相地和借景理论是乡村景观设计的核心基础,乡村天然地具有山林、江湖等自然资源,同时又因村民世代居住而流传下宗族起源和文化认同,这给予设计师难得的机会去挖掘、思考和创作。在此基础上,设计师也应该以新的语言和技术去创新和探索表达场地特征,当代而又本土的乡村景观。此外,还以皂溪村、龙坞茶村、下杨村3个案例来具体探讨实践过程中的相地之理解和借景之应用。     

Anatomical Study of the Rachis of Compsopteris elliptica and the Systematic Position of Compsopteris

The anatomical characters of the rachis of Compsopteris elliptica ex Yang et Chen are described from the calc-petrified specimens of the Late Permian of Panxing, Guizhou, China. The main characters of its rachis are as follows (Plate I, 1–4; 6–9): The vascular bundles of 2– 2.5 cycles and ectophloic type. Phloem, consisting of 1–2 layers of cells. Protoxylem, composed of several small tracheids which are less than 20μm in diameter. Metaxylem, consisting of 2–3 layers of tracheids, about 30μm in diameter, scalariform thickenning. The sclerenchyma zone lies between the vascular bundles, its cell, small, 20–25 μm in diameter, some containing brown substances. The cortex could be divided into two zones: outer zone, consisting of thin-walled cells, and inner zone, thicken-walled cells, with secretory cavities. Epidermis, one layer, rectangular, some with contents. Compsopteris sp. (Plate I,5) 3–4 cycles vascular bundles, the same as C. elliptica in many respects; only larger than C. ellipptica in diameter. It may represent the base part of the rachis or larger rachis. Based on the shape of vascular bundles and the structure of xylem and phloem, Compsopteris is very similar to Angiopteris, Danaea etc., which all belong to the Marattiales. In addition, Huang et al. (1989) found that the sporangia of Compsopteris is similar to that of Danaeites, which belongs to the Maratriales. Therefore, Compsopteris doer not belong to the seed fern, bur the Marattiales.     

草原蘑菇圈对牧草长势影响的初步分析

在白蘑,黄蘑圈上,圈内及圈外分别作测产样方,分析它们之间的差异。结果表明：白蘑,黄蘑圈均可极显著地提高群落地上生物量。其对羊草的影响较显著,圈上羊草的生物量,株高,密度及单株重均明显高于对圈内,圈外;对黄囊苔草和其它植物的影响不明显。白蘑圈对羊草的影响较黄蘑圈小。