广西桑蚕原料茧解舒率与茧层丝胶初期溶解速度的关系探讨

本文通过对不同品种、不同庄口与季节原料茧茧层丝胶性能研究,结果表明广西的桑蚕原料茧,春茧和秋茧5 min、10 min两个处理水平的茧层丝胶初期溶解速度与蚕茧解舒率之间都存在着良好的正相关关系,茧层丝胶初期溶解速度越大,蚕茧的解舒率越大.庄口和季节是影响广西桑蚕原料茧茧层丝胶初期溶解速度的重要因素.而茧层丝胶初期溶解速度与蚕茧解舒率在品种水平上则没有相关关系,也就说明茧层丝胶初期溶解速度在品种间虽然有差异,但蚕品种并不是主要制约或影响蚕茧茧层丝胶初期溶解速度的直接因素.     

广西桑蚕茧丝质量与性能指标的测定分析

本研究对广西桑蚕茧的茧丝质量与性能指标进行了测定分析.结果表明,广西桑蚕茧外形略小,茧层丝胶含量、茧层易溶丝胶含量、丝胶溶解性以及茧丝纤度等主要指标均与江浙夏秋用桑蚕茧有明显差异.桑蚕茧的茧层丝胶含量、易溶丝胶含量随庄口、季节不同而有显著差异;春季桂南地区的蚕茧茧层丝胶含量高于桂西北地区,秋季刚好相反.茧层易溶丝胶含量在11.6%～14.4%之间,约占丝胶总含量的41%～45%,茧层易溶丝胶含量和解舒率呈显著的正相关关系,即茧层易溶丝胶含量越多,蚕茧解舒越好.庄口和季节是影响桑蚕茧层易溶丝胶含量高低与蚕茧解舒优劣的重要因素.广西桑蚕茧层丝胶对煮茧温度更敏感,茧丝纤度粗细变化开差大.因此,我们认为目前困扰广西桑蚕茧加工技术突破的原因之一是缫丝工艺技术,采用传统缫丝工艺技术难以正常发挥广西桑蚕茧的茧丝优势与特性.     

中国印度茧蜂属Indiopius Fischer一新种(膜翅目:茧蜂科)

本文记述了茧蜂科Braconidae潜蝇茧蜂亚科Opiinae印度茧蜂属Indiopius的1个新种：Indiopius alutacius Weng et Chen sp.nov.。这是该属在中国分布的首次记录。建立了世界已知3种的分种检索表。本新种与矮茧蜂I.humillimus Fischer相似,但新种可以由下列特征予以区别：1.并胸腹节革质纹,后缘具短纵脊;2.第1腹节背板长为宽的1.2-1.3倍;3.腹板侧泡明显具齿。模式标本保存于福建农林大学益虫研究室。     

前裂长管茧蜂个体发育研究

前裂长管茧蜂Diachasmimorpha longicaudata（Ashmead）是许多双翅目实蝇类害虫的重要寄生性天敌。在实验室条件下,以桔小实蝇Bactrocera dorsalis（Hendel）为繁育寄主,系统观测了前裂长管茧蜂个体发育过程中各虫态的特征及其相应的发育历期。观察结果表明,前裂长管茧蜂整个生活史由卵、幼虫、蛹及成虫四个阶段组成。其幼虫可划分为四个龄期,一龄幼虫具几质丁的坚硬头壳和镰刀状的颚,形态特征与行为习性与二、三、四龄幼虫完全不同。前裂长管茧蜂约需20天完成世代发育。卵、一龄幼虫、二龄幼虫、三龄幼虫及四龄幼虫的历期依次为1．91、2．02、1．75、2．04和2．31d;蛹的历期介于8～10d,雌性个体发育时间长于雄性。前裂长管茧蜂通常比未被寄生的寄主迟3～5d羽化,雌、雄蜂在羽化后即能交配、产卵,被寄生的桔小实蝇幼虫体表上会留下明显的深褐色产卵孔,寄主化蛹后产卵孔仍保留在其蛹壳上。     

中国长柄茧蜂属亚长柄茧蜂亚属记述(膜翅目:茧蜂科)

对长柄茧蜂属Ｓｔｒｅｂｌｏｃｅｒａ亚长柄茧蜂亚属Ａｓｉａｓｔｒｅｂｌｏｃｅｒａ Ｂｅｌｏｋｏｂｙｌｓｋｉｊ（膜翅目：茧蜂科）的种类进行了研究。亚长柄茧蜂亚属仅分布于我国和俄罗斯远东地区。以前仅知２种：具角长柄茧蜂Ｓ．（Ａ．）ｃｏｒｎｕｔａＣｈａｏ,１９６４和大峪长柄茧峰Ｓ．（Ａ．）ｄａｙｕｅｎｓｉｓ Ｗａｎｇ,１９８３。本文记述了该亚属的一新种：扁角长柄茧蜂,新种Ｓ．（Ａ．）Ｐｌａｎｉｃｏｒｎｉ     

中国费氏茧蜂属新种和新记录种记述(膜翅目:茧蜂科:蝇茧蜂亚科)

本文记述了中国费氏茧蜂属的3个新种：匙胸费氏茧蜂F.mystrium,sp．nov．,锐费氏茧蜂F.oxoestos,sp．nov．,疏纹费氏茧蜂F.spanistriae,sp．nov．,和1中国新记录种：齿唇费氏茧蜂Fopius denticulifer(van Achterberg and Maeto^,1990)。这是该属在中国大陆的首次记录。1．齿唇费氏茧蜂Fopius denticulifer(van Achterberg and Maeto^,1990),中国新记录研究标本：1♀,浙江临安市天目山,1987-Ⅶ-21,陈学新,No．873495;2♀♀1♂,浙江安吉县龙王山,1993-Ⅷ-31,马云,许再福,朱春燕,No．9310360,9310128,939981;2♀♀,浙江临安市大目山,1993-Ⅵ—11,滕玲,汪建平,No．935298,935444;1♀,浙江临安市天目山,1993Ⅵ—12,马云,No．934408。分布：中国(浙江),俄罗斯东部,日本。2．匙胸费氏茧蜂Fopius mystrium,新种(图1～4)正模：♀,湖北神农架宋洛,1982-Ⅷ-28,何俊华,No．825801。鉴别特征：本新种与F.deeralensis(Fullaway,1950)相似,但下列特征可资区别：额具深密刻点及刚毛;产卵管两侧平行,端部具一小突起;产卵管鞘长度为前翅长度的0．4倍,为腹部第一背板长的2．7倍。3．锐费氏茧蜂Fopius oxoestos,新种(图5～10)正模：早,浙江德清县筏头,1995-Ⅴ-27,何俊华,No．957552;副模,1♀,浙江德清县筏头,1995-Ⅴ-27,何俊华,No．957416。鉴别特征：本新种与F.ruficornis(Granger,1949)相似,但下列特征可资区别：中胸全部橘黄色;产卵管端部背方具一突起;并胸腹节后方具一大的扇形区域。4．疏纹费氏茧蜂Fopius spanistriae,新种(图11～16)正模：♀,海南尖峰岭,1997-Ⅶ-22,蔡平,No．973369。鉴别特征：本新种与E.arisanus(Sonan,1932)相似,但下列特征可资区别：唇基腹方具一小而不明显的突起：中胸腹板后横脊腹方中央存在,短且突出;产卵管鞘相对较短,其长度为前翅长的0．8倍,为腹部第,背板长的0．9倍。     

中国小腹茧蜂属二新种记述(膜翅目,茧蜂科,小腹茧蜂亚科)

记述了采自黑龙江、吉林、辽宁和湖北省小腹茧蜂属M.icrogaster Latreille,18042新种,短管小腹茧蜂M.breviterebrae sp．nov．和长距小腹茧蜂M．longicalcar sp．nov．。短管小腹茧蜂M.breviterebrae sp．nov．(♀)与M．grandis相似,但以下特征可以区别：1)翅痣下方具暗斑(后者无);2)触角端前节长为宽的1．2倍(后者为2倍);3)头顶光滑(后者具皱纹);4)腹部第3背板光滑(后者具皱状刻点);5)后足胫节黑色(后者红黄色)。采自黑龙江镜泊湖、吉林长春、辽宁(阜新、大连、沈阳)。长距小腹茧蜂M．longicalcar sp．nov．(♀)与短管小腹茧蜂M.breviterebrae sp．nov．相似,但以下特征可以区别：1)1—RS脉长为1—M脉1／2(后者为1／3);2)r脉与翅痣宽等长(后者明显短于翅痣宽);3)后足胫节内距长为基跗节6／7(后者为1／2);4)产卵管鞘长为后足胫节1／2(后者为1／3);5)前后单眼间距与单眼直径等长(后者短于单眼直径)。采自湖北房县。本文附中国小腹茧蜂属分种检索表。模式标本均保存在浙江大学植保系寄生蜂标本室。     

中国拱茧蜂属ForniciaBrulle五新种记述：膜翅目：茧蜂科：小腹…

拱茧蜂属ＦｏｒｎｉｃｉａＢｒｕｌｌｅ是一个中小型属,过去全世界已知２１种,我国已知６种。本文报道我国发现的５个新种,它们是强突拱茧蜂Ｆ．ｐｒｏｍｉｎｅｎｔｉｃＣｈｅｎｅｔＨｅ,ｓｐ．ｎ．、弱皱拱茧蜂Ｆ．ｉｍｂｅｃｉｌｌａＣｈｅｒｅｔＨｅ,ｓｐ．ｎ．、白翅拱茧蜂Ｆ．ａｌｂａｌａｔａＭａｅｔＣｈｅｎ,ｓｐ．ｎ．、黄腹拱茧蜂Ｆ．ｆｌａｖｏａｂｄｏｍｉｎｉｓＨｅｅｔＣｈｅｎ,ｓｐ．ｎ．和小拱茧蜂Ｆ．ｍｉｎ     

中国毛室茧蜂属记述并附二新种描述（膜翅目：茧蜂科：优茧蜂亚科）

毛室茧蜂属LeiophronNees是茧蜂科优茧蜂亚科中1个较大的属,含3个亚属啮虫茧蜂亚属EuphoriellaAshmead,拟优茧蜂亚属EuphorianaGahan和毛室茧蜂亚属Leiophrons.s,寄生于半翅目和啮虫目的若虫。本文对毛室茧蜂属的中国种类进行了研究,共报道7种,其中5种为已知种程氏毛室茧蜂L.(Euphoriana)chengiChen&vanAchterberg,1997、狭翅毛室茧蜂L.(L.)bounluoicaBelokobylskij,1993、黄体毛室茧蜂L.(L.)flavicorpusChen&vanAchterberg,1997、红头毛室茧蜂L.(L.)ruficephalusChen&vanAchterberg,1997和细毛室茧蜂L.(L.)subtilisChen&vanAchterberg;2种为新种大头毛室茧蜂L.(E.)amplicaptis,sp.nov.和伊春毛室茧蜂L.(L.)yichunensis,sp.nov.。研究标本(包括模式标本)保存在浙江大学应用昆虫研究所寄生蜂标本室。 1.大头毛室茧蜂Leiophron(Euphoriana)amplicaptis,新种(图1～5) 　　本种与程氏毛室茧蜂L.(E.)chengiChen&vanAchterberg,1997相似,但前翅1-SR+M、m-cu、2-Cu1、2-M和2-SR脉缺,基节前沟缺,盾纵沟存在,浅,腹部第1背板具纵刻纹,触角15节,脸宽为高的1.1倍及体较小等特征可与后者相区别。 　　研究标本正模,♀,吉林通化,1994-Ⅷ-01,娄巨贤,no.976813。 2.伊春毛室茧蜂Leiophron(Leiophron)yichunensis,新种(图6～10) 　　本种与黄体毛室茧蜂L.(L.)flavicorpusChen&vanAchterberg,1997相似,但本种前翅SR1和2-SR脉出自翅痣同一地点、唇基腹缘明显突出、盾纵沟区域光滑、腹部第1节背板长为端宽的3.1倍等特征可与后者相区别。 　　研究标本正模,♀,黑龙江伊春,1994-Ⅶ-19～26,娄巨贤,no.952676。副模1♂,采集记录同正模,no.952608。     

中国拱茧蜂属ForniciaBrulle五新种记述（膜翅目：茧蜂科：小腹茧蜂亚科）

拱茧蜂属ＦｏｒｎｉｃｉａＢｒｕｌｌｅ是一个中小型属,过去全世界已知２１种,我国已知６种。本文报道我国发现的５个新种,它们是强突拱茧蜂Ｆ．ｐｒｏｍｉｎｅｎｔｉｓＣｈｅｎｅｔＨｅ,ｓｐ．ｎ．、弱皱拱茧蜂Ｆ．ｉｍｂｅｃｉｌｌａＣｈｅｎｅｔＨｅ,ｓｐ．ｎ．、白翅拱茧蜂Ｆ．ａｌｂａｌａｔａＭａｅｔＣｈｅｎ,ｓｐ．ｎ．、黄腹拱茧蜂Ｆ．ｆｌａｖｏａｂｄｏｍｉｎｉｓＨｅｅｔＣｈｅｎ,ｓｐ．ｎ．和小拱茧蜂Ｆ．ｍｉｎｉｓＨｅｅｔＣｈｅｎ,ｓｐ．ｎ．,并记述了２已知种在我国的新分布。文中附有我国已知种的检索表。     

End-products of behaviour versus behavioural characters: a phylogenetic investigation of pupal cocoon construction and form in some North American black flies (Diptera: Simuliidae)

Cocoon spinning was analysed, using video recording and playback, in eighteen Nearctic black fly species, comparing nine Simulium species, six Eusimulium species, Stegopterna mutata, Cnephia dacotensis and Prosimulium mixtum. Fourteen behavioural characters were revealed that produced twenty-two equally parsimonious trees (CI = 0.93, RI = 0.96). Another tree was constructed on the basis of five characters relating to the cocoon structure (end-product characters). The goal of the study was to determine whether characters relating to behavioural components of black fly cocoon spinning or those based on end-products of the behaviour are superior for revealing phylogenetic relationships. This was accomplished by comparing both data sets to a phylogeny constructed with the use of cytological and morphological characters. If taxa are grouped according to end-products (the cocoons) there are some spurious groupings. The behavioural analysis only required one extra step to duplicate the morphological and cytological tree. In the case of black flies, it is more informative to use characters resulting from the analysis of the cocoon spinning behaviour than cocoon morphology.     

QTL mapping of economically important traits in Silkworm (<Emphasis Type="Italic">Bombyx mori</Emphasis>)

A backcrossed population(BC1)was derived from a cross between C1AFLP technique was employed for mapping the QTLs.The QTLs for the whole cocoon weight,cocoon shell weight,ratio of cocoon shell,weight of pupae etc.Were analyzed and 11 QTLs were detected based on the constructed linkage map.Two QTLs for whole cocoon weight were localized on linkage group 6 and 19; three QTLs for cocoon shell weight were localized on linkage group 3,14 and 19; three QTLs for ratio of cocoon shell were localized on the linkage group 2,11and 15,and three QTLs for the weight of pupae were localized on linkage 2,14 and 19.All these have laid an important base for the marker assisted breeding of the silkworm.     

QTL mapping of economically important traits in Silkworm (Bombyx mori)

The construction of linkage map is both a funda-mental research area and an important aspect of gene analyses in genetics. It provides the guidelines for breeding. A sound linkage map is also necessary for further genetic analysis. In recent years, great and rapid progress has been made in molecular biology, which enables fingerprinting of organisms at the ge-nomic level. Many molecular marker techniques have been well established. Heartening progress has been made in many organisms in the co…     

家蚕不同茧色品种中cbp基因的结构和表达分析

类胡萝卜素结合蛋白(CBP)是唯一已被确认与家蚕黄色茧形成儡切相关的主要蛋白质。文章选择12个有色茧和白茧蚕品种, 调查了cbp基因结构、转录产物mRNA类型和丝腺类胡萝卜素紫外可见光吸收特征与其茧色的关系。结果表明: 黄色茧蚕品种含有2种或3种cbp基因结构, 同时转录具有CBP功能性的完整 mRNA和缺少第2外显子的mRNA; 绿茧品种间cbp基因结构存在差异, 转录缺少第2外显子的mRNA; 白茧蚕品种cbp基因只有1种结构, 转录缺少第2外显子的mRNA。文章在黄茧品种中新发现的cbp基因第1内含子序列可能具有茧色品种特异性, 家蚕黄茧品种丝腺的紫外可见吸收光谱特征显著区别于绿茧和白茧品种, cbp基因的结构和表达特征与家蚕茧色密切相关。     

基于超高效液相色谱-质谱技术对野蚕茧和家蚕茧化学成分进行比较

鉴定并比较野蚕茧与家蚕茧的化学成分对于理解家蚕的驯化具有重要的意义。利用高温高压结合甲醇-水提取的方法获得蚕茧中的化学成分,利用UHPLC-MS技术对野蚕、家蚕大造品种和皓月品种3种蚕茧丝中的小分子成分进行鉴定和比较分析。通过阳离子模式和阴离子模式的UHPLC-MS获得了野蚕、大造和皓月蚕茧丝的代谢指纹图谱,对鉴定到的高丰度化合物进行注释,发现其中包括了氨基酸、黄酮、生物碱、萜类、有机酸和木脂素等成分。PLS-DA的得分图表明,野蚕、家蚕大造品种和皓月品种的3种蚕茧的代谢组存在显著差异。发现脯氨酸、亮氨酸/异亮氨酸和苯丙氨酸在大造茧中的含量显著高于在野蚕和皓月茧中的含量,黄酮类植物次生代谢物在大造茧中的含量显著提高,包括槲皮素、异槲皮素、槲皮素-3-O-槐糖苷、槲皮素-3-O-L-鼠李糖苷、槲皮素-3-O-芸香糖苷和山奈酚;而神经碱、白桥楼碱、毛果芸香次碱、美洲豚草内酯、线叶泽兰素和中缅木莲素等生物碱、萜类和木脂素类的植物次生代谢物在野蚕茧中的含量显著高于在家蚕茧中的含量。在紫外光的激发下观察黄酮的绿色荧光发现家蚕大造茧中的黄酮含量最高,家蚕皓月茧中的黄酮含量最低,而野蚕茧中的黄酮含量居中。生物碱和有机酸是良好的抗虫抗菌剂,它们在野蚕茧中的含量较高,能够提高野蚕茧的防护能力。黄酮类物质在家蚕大造茧中的含量较高,是导致家蚕大造茧呈黄绿色的主要原因。     

家蚕茧质性状的QTL定位研究

采用QTLMapper 2．0 QTL作图软件,对F2群体的家蚕全茧量、茧层量、茧层率和蛹体重等性状进行了QTL定位分析,分别检测出7个、6个、2个、8个有显著效应分量的QTLs,分布于7个、5个、2个、7个不同的连锁群。控制全茧量、茧层量的QTLs一般存在复杂的上位性效应。对全茧量性状,有3对QTLs存在显著的加加上位性效应,其中1对还存在加显、显显互作;共有3个QTLs存在显著的显性效应,1个存在显著的加性效应。对茧层量QTLs,发现1对QTLs存在极显著的各项遗传效应,包括上位性效应;1对QTLs被检测到显著的显显互作,1个QTL具有显著的显性效应,并与另一个QTL存在显著的加加互作。茧层率、蛹体重主要受加性或显性的QTLs作用,没有发现茧层率QTLs的上位性效应,蛹体重的有效QTL大都呈现显著的负向显性效应,只有一对QTLs存在显著的加加上位性效应。第2、3、4、11、13、24、34、37、40连锁群是两个或多个性状QTLs分布的共同连锁群。全茧量和茧层量存在共同的QTL或染色体区域,育种上可通过适当选配,利用基因的互作效应,同步改良这两个性状。     

In-situ observation of tryptophan in larval cocoon silk of the hornet Vespa simillima xanthoptera cameron by ultraviolet resonance raman spectroscopy

We performed in-situ ultraviolet resonance Raman (UVRR) spectroscopy of the larval cocoon silk of the hornet, Vespa simillima xanthoptera Cameron, and compared the result with that of the silkworm, Bombix mori. The UVRR spectrum of the hornet cocoon differed markedly from that of the B. mori cocoon: peaks attributable to tyrosine (Tyr) were observed strongly, and tryptophan (Trp) peaks weakly, in the spectrum of the B. mori cocoon, whereas peaks attributable to Trp exclusively appeared in the spectrum of the hornet cocoon.     

Surface topology and structural integrity of the Theromyzon tessulatum (Annelida: Hirudinea: Glossiphoniidae) cocoon

Cocoons secreted by the aquatic leech Theromyzon tessulatum comprise a tubular, membranous ovoid, sealed at each end by a glue‐like substance, called an operculum. Scanning electron microscopy showed surface features of the T. tessulatum cocoon that included a circuitous bulge, cups that conformed to the shape of embryos, relief folds that radiated from opercula, and asymmetric distributions of protuberances on the upper aspect of the cocoon surface. The structural integrity of the T. tessulatum cocoon was assessed after exposure to a variety of denaturing conditions (e.g., extreme heat, detergents, acids). Although both the fibrous cocoon membrane and opercula were strikingly resilient, the membrane/operculum boundary appeared to be the weakest structural component of the cocoon, consistent with its functional role as an escape hatch for juvenile leeches. The operculum itself was more sensitive to denaturation than the cocoon membrane, and thus was probably the source of a major protein component isolated from the T. tessulatum cocoon (i.e., Tcp; Theromyzon cocoon protein). J. Morphol., 2008. © 2008 Wiley‐Liss, Inc.     

Proteins in the Cocoon of Silkworm Inhibit the Growth of Beauveria bassiana

Silk cocoons are composed of fiber proteins (fibroins) and adhesive glue proteins (sericins), which provide a physical barrier to protect the inside pupa. Moreover, other proteins were identified in the cocoon silk, many of which are immune related proteins. In this study, we extracted proteins from the silkworm cocoon by Tris-HCl buffer (pH7.5), and found that they had a strong inhibitory activity against fungal proteases and they had higher abundance in the outer cocoon layers than in the inner cocoon layers. Moreover, we found that extracted cocoon proteins can inhibit the germination of Beauveria bassiana spores. Consistent with the distribution of protease inhibitors, we found that proteins from the outer cocoon layers showed better inhibitory effects against B. bassiana spores than proteins from the inner layers. Liquid chromatography-tandem mass spectrometry was used to reveal the extracted components in the scaffold silk, the outermost cocoon layer. A total of 129 proteins were identified, 30 of which were annotated as protease inhibitors. Protease inhibitors accounted for 89.1% in abundance among extracted proteins. These protease inhibitors have many intramolecular disulfide bonds to maintain their stable structure, and remained active after being boiled. This study added a new understanding to the antimicrobial function of the cocoon.