有关生物工程专书文献分类汇集(续)

生物化学《亲和层析》(Affinity Chromatograph)一本实践入门书。P.D.G.Dean,W.S.Johnson和F.A.Middle主编。1055 IRLPress.《亲和层析》(Affinity Chromatograph)粒酸和蛋白的模板层析.H.Schott.19ss MarcelDekker.《氨基酸和肤》(Amino Aeids and Peptides)J.S.Davies 1985 Chapman and Hall.《多肤激素的生物化学》(Bioehemistry of the polypeptide Hormones)M.Wallis,5.L .Howell和K.W.Taylor,2955 John Wiley.     

The identity of some taxa of Schizophragma （Saxifragaceae）

After examining a large number of type and authoritative specimens of Schizophragma Siebold ＆ Zucc., S. elliptifolium C. F. Wei and S. integrifolium var. glaucescens Rehder were reduced to synonyms of S. hypoglaucum Rehder and S. integrifolium f. cordatum S. Y. Wang was reduced to synonym of S. corylifolium Chun. Schizophragma crassum var. ellipticum J. Anthony was treated as the correct name of S. crassum var. hsitaoanum （Chun） C. F. Wei. The name S. integrifolium var. minus Rehder is reinstated. The taxonomic treatment is supported by leaf epidermal characters.     

《现代生物医学进展》编辑部及各通联部联系方式

本刊网址 ：www.biomed.net.cn, www.shengwuyixue.com, www.swcx.periodicals.net.cn E-mail： biomed_54@126.com, liudhui_21 @126.com, biomagnetism@163.com, liudhui21 @gmail.com     

世界秋海棠属侧膜组植物新资料

记述了秋海棠属侧膜组11新种,即星果草叶秋海棠(BegoniaasteropyrifoliaY.M.ShuietW.H.Chen)、耳托秋海棠(B.auritistipulaY.M.ShuietW.H.Chen)、桂南秋海棠(B.austroguangxiensisY.M.ShuietW.H.Chen)、水晶秋海棠(B.crystallinaY.M.ShuietW.H.Chen)、须苞秋海棠(B.fimbribracteataY.M.ShuietW.H.Chen)、巨叶秋海棠(B.gigaphyllaY.M.ShuietW.H.Chen)、黄氏秋海棠(B.huangiiY.M.ShuietW.H.Chen)、长柱秋海棠(B.longistylaY.M.ShuietW.H.Chen)、扁果秋海棠(B.platycarpaY.M.ShuietW.H.Chen)、喙果秋海棠(B.rhynchocarpaY.M.ShuietW.H.Chen)、多变秋海棠(B.variifoliaY.M.ShuietW.H.Chen);并报道了3新变种及1新名称,即疏毛越南秋海棠(B.boniiGagnep.var.remotisetulosaY.M.ShuietW.H.Chen)、密毛龙州秋海棠(B.morseiIrmsch.var.myriotrichaY.M.ShuietW.H.Chen)、簇毛伞叶秋海棠(B.umbr-aculifoliaY.WanetB.N.Changvar.flocculosaY.M.ShuietW.H.Chen)及彩纹秋海棠(B.variegataY.M.ShuietW.H.Chen)。     

中国马蓝属(爵床科)新组合和新分类群(英文)

采用广义的马蓝属(Strobilanthes Blume)概念,提出3个新组合:匍匐半插花(S.primulifolia(Nees)Y.F.DengJ.R.I.Wood),直立半插花(S.cumingiana(Forst.)Y.F.DengJ.R.I.Wood)和狭叶马蓝(S.atropurpurea var.stenophylla(C.B.Clarke)Y.F.DengJ.R.I.Wood);描述了8新种:南岭马蓝(Strobilanthes austrosinensis Y.F.DengJ.R.I.Wood)、冯氏马蓝(S.fengiana Y.F.DengJ.R.I.Wood)、陶氏马蓝(S.taoana Y.F.DengJ.R.I.Wood)、启无马蓝(S.wangiana Y.F.DengJ.R.I.Wood)、景东马蓝(S.atroviridis Y.F.DengJ.R.I.Wood)、西畴马蓝(S.rostrata Y.F.DengJ.R.I.Wood)、黄连山马蓝(S.spiciformis Y.F.DengJ.R.I.Wood)和匍匐马蓝(S.procumbens Y.F.DengJ.R.I.Wood)。对南岭马蓝、黄连山马蓝、景东马蓝和匍匐马蓝的花粉形态进行了观察。     

贵州蕨类植物资料

亮鳞肋毛蕨 贵州新记录 Ctenitis subglandulosa (Hance)Ching in Bull.Fan Mem.Inst.Biol.Bot.Ser.8:302.1938;T.Nakaike,New Fl.Jap.(Rev.& Enl.)334.1992;K.Iwatsuki,Ferns & Fern All.Jap.203.t.129.f.1-3.1992;C.F.Zhang & S.Y.Zhang,Fl.Zhejiang,1:287.f.1-297.1993;C.H.Wang in S.H.Wu,Fl.Reip.Pop.Sin.6(1):36.1999.--Alsophila subglandulosa Hance in Ann.Sci.Nat.ser.5,5:253.1866.     

Taxonomic changes regarding three species of Zingiber (Zingiberaceae) from Thailand

Zingiber teres S. Q. Tong & Y. M. Xia and Z. xishuangbannaense S. Q. Tong are reduced to be synonyms of Z. smilesianum Craib and Z. thorelii Gagnep.,respectively. Z. cochinchinense Gagnep. is changed to be a subspecies of Z. zerumbet (L.) Sm.     

广西赤车属和楼梯草属杂记

<正> 赤车属Pellionia Gaud. 赤车组Sect. Pellionia 赤车系Ser. Radicantes W. T. Wang 小赤车 Pellionia minima Makino in Bot. Mag, Tokyo 23:85(1909); W. T. Wang in Bull. Bot. Lab. North-East. Forest, Inst. 6:58(1980)——Elatostema radicans var. minimum(Makino)H. Schrter in Rep. Sp. Nov. Beih, 83(2):88     

云南楼梯草属小志(英文)

Elatostema integrifolium (D. Don) Wedd. var. tomentosum (Hook. f.) W. T. Wang, comb. nov.——E. sesquifolium (BI)Hassk. var. tomentoaum Hook. f. Fl. Brit. Ind. 5:565 (1888); W. T. Wang in Bull. Bot. Lab. North-East. Forest. Inst. 7:84(1980).     

藏药雪莲花的化学成分

菊科植物丛株雪兔子(Soussurea tridactyla Sch.-Bip. var. maidugonla S. W. Liou)为西藏藏族药物雪莲花的原植物之一,从中分离到九种化学成分,分别鉴定为芹菜索,芹菜素-7-葡萄糖甙,伞形花内酯,伞形花内酯-7-葡萄糖甙,东莨若素,对羟丛苯乙酮,3-吲哚乙酸,秋水仙碱及β-谷甾醇。     

利用叶绿体DNA trnL-F序列初步探讨菊科风毛菊属的系统发育

风毛菊属Saussurea DC.是菊科物种分化十分剧烈和分类处理十分困难的一个属。该属的单系起源性质、属下分类系统以及一些独特形态物种的系统位置尚不清楚,有待进一步验证。本文测定了代表该属5个亚属37种植物43个样品和川木香属Dolomiaea DC.的１种样品的叶绿体DNA trnL-F序列,并调取菜蓟族Cardueae Cass.与风毛菊属具有一定亲缘关系的13属的该序列,一起进行了分支分析,重点验证该属的属下形态分类系统以及形态特殊、青藏高原地区特有的雪兔子亚属subgen. Eriocoryne     

Comparison of Differences in Chemical Composition and Related Antioxidant Activity of Snow Lotus from Different Origins

The snow lotus is an endangered traditional Chinese medicinal herb. Saussurea involucrata, Saussurea laniceps, and Saussurea medusa, the three main snow lotus species (five herbs and two S. involucrata cell cultures), were selected for this study. Snow lotus (XLs) was extracted using 75 % (v/v) ethanol. Two reversed phase-high performance liquid chromatography-diode array detector methods were developed and validated for the determination of 10 representative components in XLs. The antioxidant efficacy of XLs and their components was investigated using DPPH, ABTS free radical scavenging, and ROS inhibition experiments. The results showed that the IC₅₀ for DPPH scavenging ranged from 0.06–0.29 mg/mL for XLs and from 0.13–0.63 mg/mL for ABTS, and could downregulate ROS to varying degrees. The results of the antioxidant activity showed that rutin, quercetin, and isochlorogenic acid A contributed to the antioxidant capacity of XLs. The high content and activity of the cell cultures indicate that they can serve as an effective alternative to snow lotus, thus providing a theoretical basis for the selection of herbs and cell cultures to fulfil various needs.     

尖苞雪莲的化学成分

利用硅胶柱层析、Sephadex LH-20和MCI等分离和纯化手段,从产于青海的菊科风毛菊属植物尖苞雪莲的全草中,分离得到5个化合物,经IR、NMR(1D、2D)、MS等现代波谱技术鉴定它们为芦丁、槲皮素-5-O-β-D-葡萄糖苷、丁香苷、胡萝卜苷和β-谷甾醇,其化学成分为首次报道。     

雪莲PBP基因表达载体的构建

目的:利用新疆雪莲特殊功能基因磷脂酰乙醇胺结合蛋白基因(XLPBP)与基础质粒构建植物表达载体pXLPBP,为介导该基因在植物中表达,以期提高植物抗寒力的转基因研究打下基础。方法:利用设计好的两端加有EcoRⅠ酶切位点的引物,对XLPBP全长基因片段进行PCR扩增,获得700bp左右大小的片段,将其纯化回收并与同样经过EcoRⅠ酶切的质粒pCAMBIA3301连接;然后采用冻融法和电击法,将含XLPBP基因的载体pCAMBIA3301转入根癌农杆菌中。结果:通过一系列分子克隆方法获得含雪莲XLPBP基因的植物表达载体,并经PCR实验证实。结论:利用以自身携带的非编码区为调控序列的XLPBP全长基因和双向表达载体pCAMBIA3301为基础构建植物表达载体,可望提高外源基因表达量。     

Saussurea xinjiangensis sp. nov. (Asteraceae,Cardueae), a new species from Xinjiang,China

Saussurea xinjiangensis Y. S. Chen, a new species from Xinjiang, China, is described and illustrated. It was first found during a field expedition by the author in 2014. It belongs to S. subgen. Saussurea sect. Laguranthera because its root and caudex split fibrously and it has numerous capitula in a corymbiform synflorescence. The new species is similar to S. mucronulata in its habit, strongly fibrous rootstock and caudex, sessile linear leaves and capitula shortly pedunculated in a corymbiform synflorescence. However, it differs from S. mucronulata by its usually branched stems (versus usually simple or apically few-branched), lower leaf blade 8–12 cm long and 0.5–1.8 cm wide (versus 3–5 cm long and 0.2–0.5 cm wide), leaves abaxially sparsely arachnoid tomentose (versus densely arachnoid tomentose), phyllary white arachnoid tomentose and green to brown (versus glabrous and blackish purple).     

秋水仙素诱导天山雪莲四倍体的研究

利用组织培养附加秋水仙素的方法对濒危药用植物天山雪莲(Saussurea involucrata Kar.et Kir.)进行多倍体诱导,结果表明:(1)与种子作材料相比,采用秋水仙素处理无菌苗是获得天山雪莲多倍体植株的最佳途径.(2)二倍体植株的叶片薄、平展、较细长、色绿、尖齿不明显、根细长;经秋水仙素处理诱导得到的植株叶片变宽、变肥厚、叶色深绿,尖齿明显、根短粗.(3)染色体计数、气孔分析及流式细胞仪倍性综合检测表明,诱导得到的多倍体变异苗为纯合四倍体.     

STUDY ON CHEMICAL CONSTITUENTS OF SAUSSUREA LAPPA II

Seven compounds were isolated from the underground parts of Saussurea lappe in-cluding three steroids, pregnenolone (1), β-sitosterol (2), daucosterol (3); a phenylpropanoid glycoside, syringin (4); a lignan glycoside, l- hydroxypinoresinol-l-β- D- glucopyrano side (5); a straight chain fatty acid and an its propanedinyl ester, (z, z) -9, 12 -octadecadienoic acid (6), (z,z)- 9, 12 -octadecadienoic acid -2- hydroxy -1, 3 -propanedinyl ester (7). The structures of these compounds were determined by means of spectral and some chemical evidence. Moreover, among the seven compounds, compound 1 as a natural product has been isolated from plantS for the first time, compounds 3,4,5 and 7 have been obtained from this plant source for the first time.     

雪莲类PEBP基因在大肠杆菌中的表达及鉴定

本实验旨在构建雪莲类PEBP基因原核表达载体,在大肠杆菌中表达并纯化类PEBP基因所编码的蛋白,为进一步研究奠定基础.将雪莲类PEBP基因开放阅读框序列克隆到原核表达载体pET30( )上,转化感受态表达菌株BL21(DE3),低浓度IPTG低温诱导融合蛋白的表达,纯化产物,Western blotting鉴定目的蛋白.IFTG低诱导PEBP,经SDS-PAGE分析,其相对分子量约为28 kD,与预期相符,表达量约占菌体蛋白的26.8%,并且通过亲和层析纯化了重组融合蛋白,Western blotting鉴定为阳性.成功构建了原核表达载体pET-PEBP,获得了高效表达产物,并为进一步研究雪莲类PEBP基因的抗冻功能打下基础.