玉米农田空气动力学参数动态及其与影响因子的关系

对锦州玉米农田生态系统生长季内的粗糙度(z0)和零平面位移(d)动态特征及其与风速、株高(h)和叶面积指数(LAI)的关系分析表明:零平面位移(d)在玉米拔节后10d左右,株高约1.40m时开始出现,在抽雄前为0.80-1.00m,抽雄后为1.00-1.40m;z0在抽雄前小于0.20m,乳熟前后达到最大,约为0.40m.d/h和z0/分别为0.40-0.54和0.10-0.14,在h达到最大值前,分别随LAI增大而减小和增大.d出现前,z0与风速呈负指数关系,与LAI和h都呈线性关系.d出现后,风速与z0、d之和的关系比与各自的关系更显著;d与LAI、h都呈显著指数正相关关系,LAI、h对z0的影响大于d和z0 +d,且h的作用大于LAI.d出现至h达到最大期间,上述各种关系最为显著.研究结果可为建立空气动力学参数的动态参数化方案及改进陆面过程模型提供依据.     

盘锦芦苇湿地空气动力学参数动态及其影响因子

应用盘锦芦苇生态系统观测场的涡动相关通量和小气候梯度系统的观测资料,采用涡动相关与小气候梯度观测相结合的方法估算了研究区芦苇下垫面空气动力学参数,并分析了其影响因素.结果表明：该方法可较好地估算芦苇湿地的空气动力学参数;芦苇湿地零平面位移（d）和粗糙度（z0）的季节变化均呈先增后减的单峰曲线变化.z0和d均在9月达到最大,分别为0.24和1.85m;12月达最小,分别为0.03和0.02m.芦苇湿地z0和d的季节变化主要受株高（h）和叶面积指数（LAI）的影响.d随h的增加而线性增加,z0与h呈二次曲线关系.d/h和z0/h均与LAI呈幂函数关系,复相关系数分别为0.99和0.78.     

动态空气动力学参数对玉米田陆-气通量模拟的影响——以BATSle模型为例

基于2006-2008年锦州玉米农田生态系统的通量、气象及生物因子连续观测,利用所建立的考虑玉米不同发育期叶面积指数、冠层高度和风速的地表粗糙度(z0)和零平面位移(d)动态参数化方案对BATS1e陆面模型进行改进,研究空气动力学参数变化对玉米田陆-气通量模拟的影响.结果表明:改进后的拖曳系数(CD)随植被覆盖度增大而增大,更符合实际;感热、潜热和土壤热通量的模拟精度均有不同程度改进,效率系数分别提高0.0569、0.0194和0.0384,生长季累计改进量分别占总辐射的0.9％、1.1％和1.2％;当输入真实的表层土壤湿度后,z0和d动态参数化对感热和潜热的改进作用更大.合理的动态空气动力学参数化方案对陆面过程模拟具有明显改善作用.     

盘锦芦苇湿地空气动力学参数动态及其影响因子

应用盘锦芦苇生态系统观测场的涡动相关通量和小气候梯度系统的观测资料,采用涡动相关与小气候梯度观测相结合的方法估算了研究区芦苇下垫面空气动力学参数,并分析了其影响因素.结果表明：该方法可较好地估算芦苇湿地的空气动力学参数;芦苇湿地零平面位移（d）和粗糙度（z₀）的季节变化均呈先增后减的单峰曲线变化. z₀和d均在9月达到最大,分别为0.24和1.85 m; 12月达最小,分别为0.03和0.02 m.芦苇湿地z₀和d的季节变化主要受株高(h)和叶面积指数(LAI)的影响. d随h的增加而线性增加,z₀与h呈二次曲线关系.d/h和z₀/h均与LAI呈幂函数关系,复相关系数分别为0.99和0.78.     

通用风速廓线模型在山区森林的适用性

基于莫宁-奥布霍夫相似理论的解析方程(风速廓线模型)自20世纪70年代以来一直被用于平坦地形上的风速预测,成熟且准确,但在非平坦地形适用性尚不清楚。其适用性标志着对非平坦地形近地层空气动力学参数(如零平面位移高度及粗糙度)估计的准确性,将决定基于涡度协方差法计算碳水及痕量气体通量中频率修正及其源区分析的准确性。因此,风速廓线模型在非平坦地形的适用性评价是确认通量模型是否需要改进的前提。本研究利用辽东山区清原森林站科尔塔群测量三维风速数据,并与辽河平原盘锦农田站同类数据相比较,评估了以莫宁-奥布霍夫相似理论为基础建立的通用风速廓线模型在非平坦地形上的适用性。结果表明：通用风速廓线模型无法准确预测清原森林站3座通量塔所在地的风速,不适用于复杂地形。在非生长季和生长季,清原森林站3座通量塔实际观测与模型预测风速的决定系数(R²)为0.12～0.30,观测数据与预测值的标准误超过2 m·s^-1,模型风速约为实际观测风速的2.0倍,显著高估。然而,该模型可准确预测盘锦农田站平坦农田景观的水平风速,实际观测风速与模型预测之间的R²为...     

森林蒸散模型参数的确定

以长白山阔叶红松林为研究对象,利用长白山阔叶红松林气象观测塔安装的常规气象梯度观测系统、开路涡动相关系统的观测数据,依据空气动力学基本理论与能量平衡方程建立了森林蒸散机理模型,确定了模型参数,即风速廓线稳定度订正函数ψm、温度廓线稳定度订正函数ψh和零平面位移高度d。其中,研究地的零平面位移高度d为17．8m,是平均冠层高度(26m)的0．68。同时,给出了ψm和ψh随梯度理查逊数Ri变化的数学表达式。     

稀疏植被区空气动力学粗糙度特征及遥感反演

应用中国西北半干旱区定西试验站2005年观测资料,估算了典型的稀疏农作物区空气动力学粗糙度,结合空气动力学模型分析了稀疏植被区空气动力学粗糙度的时间变化及其与风速、植株密度的关系,并得出空气动力学粗糙度的参数化方案;结合卫星遥感得到的植被叶面积指数资料,模拟了区域空气动力学粗糙度,得出:(1)空气动力学粗糙度的时间变化与风速变化规律有相反的特点;在稀疏植被区,粗糙单元分布密度对流场有一定影响,随粗糙单元密度的增加,空气动力学粗糙度将逐渐增大。(2)植株高度超过0.2m时,植被高度和植株密度、叶面积指数对空气动力学粗糙度的影响较大,当植株高度低于0.2m时,风速的影响较大。(3)结合遥感反演的叶面积指数,采用空气动力学粗糙度和叶面积指数及风速的关系,反演了空气动力学粗糙度,反演结果空间分布和误差都反映了实际空气动力学粗糙度的特征,证明空气动力学粗糙度遥感参数化比较成功。     

1982-2013年内蒙古地区植被物候对干旱变化的响应

气候变化引起的植被物候变化正在大幅度改变生态系统,研究植被物候对干旱的响应对保护内蒙古的生态系统具有重要意义。根据1：100万植被区划,把内蒙古划分为8个植被分区,利用多时间尺度气象标准化降水蒸散指数（SPEI）和NDVI3g时序数据所反演的物候指标,分析内蒙古植被物候的时空变化及其对干旱的响应规律。结果显示：1）在1982年至2013年间,内蒙古植被受到不同时间尺度下干旱的高度控制,尤其是时间尺度干旱的影响（SPEI-3）;2）对于整个研究区,生长季开始（SOS）呈提前趋势,生长季结束（EOS）呈延后趋势,生长季长度（LOS）呈延长趋势,像元比例分别为63.79%、59.77%和62.83%;3）内蒙古除荒漠植被类型地区外,同年春季和夏季初期干旱对SOS均具有延迟作用,同年秋季干旱对EOS均具有延迟作用 ;4） 不同植被类型对干旱强度指数的响应程度存在差异,响应程度集中在-10d/0.1-10d/0.1（例如,1d/0.1表示干旱强度指数每增大0.1,会导致物候指数延迟1 d,而-1d/0.1表示干旱强度指数每增大0.1,会导致物候指数提前1 d）。     

西北温凉半湿润区气候变化对马铃薯生长发育的影响——以甘肃岷县为例

利用西北温凉半湿润区马铃薯生长发育定位观测资料、加密观测和对应平行气象观测资料,分析气候变化对马铃薯生长发育的影响,以及马铃薯块茎生长与气象条件的关系。结果表明,研究区域降水量年际变化呈下降趋势,降水量变化曲线线性拟合倾向率为-8.329 mm/10 a。降水量存在3 a的年际周期变化。气温年际变化呈上升趋势,气温变化曲线线性拟合倾向率为0.144℃/10 a。作物生长季干燥指数呈显著上升趋势,干燥指数变化曲线线性拟合倾向率为0.042/10 a,20世纪90年代初至2007年明显趋于干旱化。马铃薯播种到采收约需150-168d,需≥0℃积温2000-2300℃,降水量400-500mm,日照时数900-1100h。马铃薯在播种后105d开始,块茎由缓慢生长转为迅速生长阶段;在播种后127d,块茎生长速度最大;播种后149d开始,块茎生长从迅速生长又转为缓慢生长。对马铃薯生长发育全生育期而言,受气候变暖的影响,马铃薯花序形成期每10 a提前8-9d,开花期每10 a提前4-5d。气温对马铃薯产量形成除采收期外,其余为负效应,块茎膨大期对气温变化十分敏感;而降水量的影响函数同热量的影响函数呈反相位分布,除出苗期和采收期降水量为负效应外,其余时段降水量对马铃薯产量形成均为正效应,马铃薯分枝期到开花期对降水量变化十分敏感。     

长白山红松针阔叶混交林林冠层叶面积指数模拟分析

根据长白山原始红松针阔叶混交林光合有效辐射的连续3年自动观测结果,结合便携式叶面积仪的季节观测,建立了以林冠上下光合有效辐射估算森林冠层叶面积指数的半经验公式.结果表明,该方法可以很好地反映叶面积的季节动态. 通过3年叶面积指数的季节动态比较发现,该森林冠层叶面积的起始生长日期随气温稳定通过0 ℃的日期延迟而延迟,整个生长季的叶面积动态可划分成上升期、相对稳定期和下降期,每个阶段都与大于0 ℃的积温存在较好的相关关系,分别用Logistic曲线和线性方程表达. 并对叶面积的观测和估算方法中存在的问题进行了讨论.     

壳聚糖酶的克隆表达与壳寡糖的制备分析

目的:克隆壳聚糖酶基因于大肠杆菌中实现高表达,制备壳寡糖。方法:以枯草芽孢杆菌总DNA为模板扩增壳聚糖酶基因(CSN),克隆至载体pET23a(+)上,转化菌株BL21(DE3)。重组子经0.5 mmol/L IPTG诱导后,SDS-PAGE和质谱检测与鉴定重组酶。酶纯化后水解壳聚糖,薄层色谱分析其水解产物。结果:质谱证明壳聚糖酶(31.5kDa)成功表达,表达量占菌体总蛋白的45%左右。纯化后重组酶浓度为900 mg/L,纯度95%、回收率85%,酶活力为10 000 U/mg。壳聚糖降解产物为壳二糖至壳四糖。结论:原核表达载体pET23a(+)-CSN构建正确,壳聚糖酶表达量与活性高,适用于水解壳聚糖制备壳寡糖。     

国内外蝗害治理技术现状与展望

本文首先概述了国内外蝗虫发生与为害的态势,总结了现阶段我国蝗虫发生与为害的主要特点:即农田飞蝗暴发频繁而且严重,草原土蝗的发生时常造成严重的经济损失,而且侵入城市干扰市民生活,我国与周边国家之间蝗虫过境迁移频繁,使用化学农药污染环境和农产品;分析了国内外蝗虫防治对策与技术的发展现状,重点介绍了应急防治和可持续治理对策、...     

Coiled-coil nanomechanics and uncoiling and unfolding of the superhelix and alpha-helices of myosin

The nanomechanical properties of the coiled-coils of myosin are fundamentally important in understanding muscle assembly and contraction. Force spectra of single molecules of double-headed myosin, single-headed myosin, and coiled-coil tail fragments were acquired with an atomic force microscope and displayed characteristic triphasic force-distance responses to stretch: a rise phase (R) and a plateau phase (P) and an exponential phase (E). The R and P phases arise mainly from the stretching of the coiled-coils, with the hinge region being the main contributor to the rise phase at low force. Only the E phase was analyzable by the worm-like chain model of polymer elasticity. Restrained molecular mechanics simulations on an existing x-ray structure of scallop S2 yielded force spectra with either two or three phases, depending on the mode of stretch. It revealed that coiled-coil chains separate completely near the end of the P phase and the stretching of the unfolded chains gives rise to the E phase. Extensive conformational searching yielded a P phase force near 40 pN that agreed well with the experimental value. We suggest that the flexible and elastic S2 region, particularly the hinge region, may undergo force-induced unfolding and extend reversibly during actomyosin powerstroke.     

放牧对牧草光合作用、呼吸作用和氮、碳吸收与转运的影响

研究放牧对草地植物生理活动的影响,对于揭示草地放牧演替的生理机制有重要意义.大量研究表明,家畜放牧对牧草光合作用、呼吸作用以及C和N吸收与转运的影响,可以分为生理伤害和生理恢复2个阶段.放牧通过改变草地冠层结构影响牧草光合作用,净光合作用速率短期内迅速下降,随着叶面积指数增加又逐渐上升,呼吸作用有相似的变化趋势.牧草放牧后再生长所需的C和N最初主要来自根系和留茬中的贮藏物质,此后随着牧草生长恢复逐渐由同化作用供给,C代谢与土壤N水平负相关.放牧后牧草生理活动变化与牧草遗传特性、种间竞争、家畜放牧特征、非生物环境等因素密切相关.     

Synthesis of hapten and conjugates of coumestrol and development of immunoassay

3-O-Carboxymethylcoumestrol was prepared as the hapten for immunoassay by a partial alkylation of coumestrol with ethyl chloroacetate in acetone alkalized with potassium carbonate. 3-O-Ethoxycarbonylmethylcoumestrol was separated by column chromatography and finally was hydrolyzed with formic acid. 1H and 13C NMR data (APT, COSY, HMQC, and HMBC) revealed that the reaction was regioselective, as 3-O-ethoxycarboxymethylcoumestrol was the only monosubstituted derivative. The hapten was then conjugated to bovine serum albumin and used for immunization of rabbits. A radioimmunoassay (RIA) system was established based on the polyclonal antiserum and a 125I-labeled hapten-tyrosine methyl ester conjugate as the radioligand. Parameters of the RIA: sensitivity: 12 pg per tube, 50% intercept: 140 pg per tube, working range: 20-4000 pg per tube. The cross-reactivity of a panel isoflavonoid and lignan phytoestrogens was either negligible (e.g. formononetin 0.07%; biochanin A 0.06%) or not detectable at all. The major immunoreactive peak in HPLC fractions from an alfalfa extract had the same retention time as coumestrol standard and represented 94.8% of the signal. The remaining 5.2% of immunoreactivity was distributed between five minor peaks. We conclude that after the validation for particular matrices, the method will be a useful tool for analysis of coumestrol, especially in low volume and low concentration samples.     

Kinetics and thermodynamics of peroxidase- and laccase-catalyzed oxidation of N-substituted phenothiazines and phenoxazines

N -substituted phenothiazines (PTs) and phenoxazines (POs) catalyzed by fungal Coprinus cinereus peroxidase and Polyporus pinsitus laccase were investigated at pH 4–10. In the case of peroxidase, an apparent bimolecular rate constant (expressed as k _cat/K _m) varied from 1 ×10⁷M⁻¹s⁻¹to 2.6×10⁸M⁻¹s⁻¹ at pH 7.0. The constants for PO oxidation were higher in comparison to PT. pH dependence revealed two or three ionizable groups with pK _a values of 4.9–5.7 and 7.7–9.7 that significantly affected the activity of peroxidase. Single-turnover experiments showed that the limiting step of PT oxidation was reduction of compound II and second-order rate constants were obtained which were consistent with the constants at steady-state conditions. Laccase-catalyzed PT and PO oxidation rates were lower; apparent bimolecular rate constants varied from 1.8×10⁵M⁻¹s⁻¹ to 2.0×10⁷M⁻¹s⁻¹ at pH 5.3. PO constants were higher in comparison to PT, as was the case with peroxidase. The dependence of the apparent bimolecular constants of compound II or copper type 1 reduction, in the case of peroxidase or laccase, respectively, was analyzed in the framework of the Marcus outer-sphere electron-transfer theory. Peroxidase-catalyzed reactions with PT, as well as PO, fitted the same hyperbolic dependence with a maximal oxidation rate of 1.6×10⁸M⁻¹s⁻¹ and a reorganization energy of 0.30 eV. The respective parameters for laccase were 5.0×10⁷M⁻¹s⁻¹ and 0.29 eV. Received: 20 September 1999 / Accepted: 24 February 2000     

白术同源四倍体的诱导和鉴定及其与二倍体过氧化物酶的比较

以白术（Atractylodes macrooephala Koidz.）二倍体组培苗为材料,对其四倍体诱导方法进行研究,共获得45个白术同源四倍体株系,为优良株系的选育提供了材料。此外,还分析比较了其中8个白术四倍体株系与二倍体的过氧化物酶同工酶（POD）的酶谱差异,发现四倍体各株系过氧化物酶同工酶谱比二倍体的均多了Rf0.310的谱带,且总过氧化物酶比活力也发生了很大改变,对探讨白术四倍体优良株系的生理生化机理具有一定的参考价值。     

Morphology and putative function of the colon and cloaca of marine and freshwater snakes

Among tetrapods, evidence for postrenal modification of the urine by the distal digestive tract (including the colon and cloaca) is highly variable. Birds and bladderless reptiles are of interest because the colon and cloaca represent the only sites from which water and ions can be reclaimed from the urine secreted by the kidney. For animals occupying desiccating environments (e.g., deserts and marine environments), postrenal modification of the urine may directly contribute to the maintenance of hypo‐osmotic body fluids. We compared the morphology and distribution of key proteins in the colon, cloaca, and urogenital ducts of watersnakes from marine (Nerodia clarkii clarkii) and freshwater (Nerodia fasciata) habitats. Specifically, we examined the epithelia of each tissue for evidence of mucus production by examining the distribution of mucopolysaccharides, and for evidence of water/ion regulation by examining the distribution of Na⁺/K⁺‐ATPase (NKA), Na⁺/K⁺/Cl^? cotransporter (NKCC), and aquaporin 3 (AQP3). NKCC localized to the basolateral epithelium of the colon, urodeal sphincter, and proctodeum, consistent with a role in secretion of Na⁺, Cl^?, and K⁺ from the tissue, but NKA was not detected in the colon or any compartment of the cloaca. Interestingly, NKA was detected in the basolateral epithelium of the ureters, suggesting the urothelium may play a role in active ion transport. AQP3 was detected in the ureters and coprodeal complex, consistent with a role in urinary and fecal dehydration or, potentially, in the production of the watery component of the mucus secreted by the coprodeal complex. Since no differences in general cloacal morphology, production of mucus, or the distribution of ion transporters/water channels were detected between the two species, cloacal osmoregulation may either be regulated by proteins not examined in this study or may not be responsible for the differential success of N. c. clarkii and N. fasciata in marine habitats. J. Morphol. 2011. © 2011 Wiley Periodicals, Inc.