不同海拔‘凤丹'牡丹籽粒油脂品质形成及代谢相关基因差异表达

通过对洛阳地区海拔100、650和1010 m‘凤丹'牡丹籽粒发育过程中形态指标、营养成分和关键基因的表达分析,研究了不同海拔条件下‘凤丹'牡丹籽粒产量性状变化和可溶性糖、淀粉、可溶性蛋白质与脂肪酸间的转化规律,以及相关酶活性和油脂代谢关键基因差异表达。结果表明: ‘凤丹'牡丹单果籽粒产量性状随海拔的升高而升高,且高海拔‘凤丹'籽粒生长期长于低、中海拔。成熟籽粒中可溶性糖和淀粉含量随海拔的升高而增加,可溶性蛋白质和粗脂肪含量差异不显著。籽粒发育过程中蔗糖合成酶(SS)和蔗糖磷酸合成酶(SPS)活性呈现先降后升的趋势,花后90 d活性最低;丙酮酸脱氢酶(PDH)、谷丙转氨酶(GPT)和谷草转氨酶(GOT)活性均在花后50～90 d快速增加,花后90 d达到最大值。牡丹籽粒中乙酰CoA羧化酶基因(ACCase)和硬脂酰ACP脱氢酶基因(SAD)相对表达量在花后50 d达到最大值,而ω-6脂肪酸去饱和酶2(FAD2)相对表达量在花后90 d达到最大值。籽粒发育过程中可溶性糖和淀粉与蛋白质和粗脂肪的积累呈负相关;SPS活性与可溶性糖和淀粉呈正相关,与可溶性蛋白质和粗脂肪呈极显著负相关;GPT、GOT与可溶性糖呈负相关,与淀粉呈极显著负相关,与可溶性蛋白质和粗脂肪呈极显著正相关;PDH与可溶性蛋白质、GPT、GOT呈正相关,与可溶性糖呈负相关,与淀粉呈极显著负相关。表明牡丹籽粒发育过程中养分积累是由糖类向粗脂肪和蛋白质的方向转化,SPS、PDH、GPT、GOT等在此过程中起重要作用。籽油中的棕榈酸、硬脂酸和亚油酸与亚麻酸相对增量呈负相关,说明牡丹籽油中脂肪酸去饱和过程是向亚麻酸合成的方向进行。ACCase、SAD和FAD2相对表达量与亚麻酸的相对增量呈正相关,均在亚麻酸合成过程中起重要作用。不同海拔条件下牡丹籽粒油脂品质相对稳定,籽粒生产性能随海拔的升高而升高,故在洛阳中高海拔地区种植油用牡丹是边际土地高效利用的重要策略。     

果梅果实发育过程中有机酸含量及相关代谢酶活性的变化特征

以优选荔波野生梅单株‘荔波-3’和‘荔波-11’为试验材料,采用高效液相色谱法(HPLC)测定了果梅果实在发育过程中有机酸含量及相关代谢酶活性变化特征。结果表明:(1)‘荔波-3’和‘荔波-11’果梅果实中柠檬酸和苹果酸主要在果实发育后期积累,花后140d两品种的柠檬酸积累量达到最大,且果实中总酸的积累量也相应的达到最大值。(2)柠檬酸积累量与柠檬酸合成酶(CS)活性呈极显著正相关关系,‘荔波-11’因CS活性高于‘荔波-3’,使得其果实成熟时的柠檬酸含量高于后者。(3)苹果酸积累量与磷酸烯醇式丙酮酸羧化酶(PEPC)和NAD-苹果酸脱氢酶(NAD-MDH)活性呈极显著正相关关系,但与NADP-苹果酸酶(NADP-ME)活性呈负相关关系;在花后45~100d内,果梅果实中PEPC和NAD-MDH活性迅速升高,而NADP-ME活性相对较低,使苹果酸积累量迅速增加;在花后120~140d,‘荔波-3’果实中PEPC、NADP-MDH活性显著高于‘荔波-11’,而其NADP-ME活性低于‘荔波-11’,导致成熟时‘荔波-11’的苹果酸含量高于‘荔波-3’。可见,果梅果实的有机酸主要在果实发育后期积累,有机酸积累差异受多个酶协同调控;苹果酸的积累差异主要由NAD-MDH、PEPC和NADP-ME活性协同变化引起,柠檬酸积累差异主要受CS活性变化影响。     

灌浆期间不同穗型冬小麦品种源库端的碳氮化合物含量及与其相关的酶活性变化

灌浆期间冬小麦多穗型品种豫麦49和大穗型品种豫麦66旗叶中蔗糖磷酸合成酶活性和可溶性糖含量变化均呈单峰曲线,后者的峰值出现较晚,灌浆中后期叶片中相关的酶活性下降缓慢。两品种旗叶中硝酸还原酶活性变化趋势基本相同,开花后5～20d问,豫麦49旗叶中NR活性高于豫麦66,20～35d间情况相反。在整个灌浆期间豫麦49籽粒淀粉积累速率呈双峰曲线,峰值分别出现在花后15～20d和25～30d问;豫麦66则为单峰曲线,峰值出现较晚但持续时间较长。     

网纹甜瓜发育果实糖分积累与蔗糖代谢参与酶的关系

随着网纹甜瓜果实的发育,果实中葡萄糖和果糖的含量增加,蔗糖的快速积累发生在果实发育的中后期,高蔗糖积累型果实中蔗糖积累速率明显快于低蔗糖积累型.蔗糖磷酸合成酶活性在果实发育的前期短暂下降, 而后稳步上升,在果实发育的中后期高蔗糖积累型果实中该酶的活性显著高于低蔗糖积累型果实;随着果实发育,蔗糖合成酶的分解活性降低而合成活性升高.酸性和中性转化酶在未成熟果实中活性较高,而在成熟果实中很低; 高蔗糖积累型果实中酸性转化酶活性显著低于同期低蔗糖积累型果实.合成蔗糖的酶活性小于分解蔗糖的酶活性时蔗糖几乎没有积累.根据这些结果推测,转化酶活性的下降、蔗糖磷酸合成酶活性的增加以及蔗糖合成酶分解活性的下降和合成活性的增加,是引起果实蔗糖积累的主要内在因子.     

糯性和非糯性小麦灌浆期胚乳直/支链淀粉积累及其相关酶活性研究

选用3份糯性和2份非糯性小麦材料,通过田间试验在灌浆过程中分别检测了各材料的籽粒直链和支链淀粉积累量、淀粉积累速率及淀粉合成关键酶活性的动态变化过程,探讨籽粒淀粉累积与相关酶活性的关系.结果表明:(1)非糯小麦在花后7 d前均未检测到直链淀粉存在,而此时已经检测到支链淀粉含量,并且糯小麦仅含有支链淀粉,支链淀粉早于直链淀粉合成.(2)糯性和非糯性小麦灌浆期籽粒的直、支链淀粉积累速率均呈先增加后降低的趋势,且直、支链淀粉最终积累量取决于最大积累速率和平均积累速率的大小,而积累活跃期的调节作用较小;糯性和非糯性小麦在淀粉合成过程中的腺苷二磷酸葡萄糖焦磷酸化酶(AGPP)、可溶性淀粉合成酶(SSS)、颗粒结合型淀粉合成酶(GBSS)和淀粉分支酶(SEB)活性均呈单峰曲线变化,活性峰值基本上都出现在花后20～25 d左右.(3)直链淀粉积累速率与AGPP、SSS、GBSS和SBE活性变化显著或极显著正相关,而支链淀粉积累速率仅与SSS活性变化极显著正相关,总淀粉积累速率与AGPP和SSS活性变化显著或极显著正相关.     

昼间亚高温对花后番茄叶和果实中光合产物代谢的影响

于日光温室中栽培的番茄第一花序第一花开花和开花10d时分别作昼间35℃亚高温处理(以25℃为对照),检测不同生育期的番茄叶与果实中同化产物积累与代谢变化的结果表明:番茄开花后叶中果糖、葡萄糖和蔗糖含量均下降,花后亚高温处理时期越早,叶中糖含量越低;果实发育过程中的果糖、葡萄糖和蔗糖含量比25℃下的高,但成熟时趋于一致。叶和果实中蔗糖代谢相关酶的活性变化基本一致,酸性转化酶(AI)与中性转化酶(NI)活性下降,蔗糖合成酶(SS)和蔗糖磷酸合成酶(SPS)活性升高。     

橄榄果实发育成熟过程中糖积累与相关酶活性的关系

以可溶性总糖含量差异明显的2个橄榄品种为试验材料,测定果实发育成熟过程中蔗糖、葡萄糖、果糖、可溶性总糖含量及蔗糖代谢相关酶活性的动态变化,并对果实糖积累与酶活性进行相关性分析,以明确不同橄榄品种果实糖积累差异的生理基础,为进一步在代谢与分子水平探讨橄榄果实糖积累机制提供依据。结果表明:(1)蔗糖快速积累期是橄榄品种间果实蔗糖积累差异的关键时期,并影响成熟时果实可溶性总糖含量的高低,其中‘马坑22’蔗糖快速积累期较长,增长幅度较大,成熟时可溶性糖含量高;成熟时‘马坑22’、‘檀头23’果实内己糖与蔗糖比分别为0.668、0.904。(2)在蔗糖快速积累期内,‘马坑22’酸性转化酶(AI)活性低于‘檀头23’,为其蔗糖积累创造条件,而中性转化酶活性高于后者则有利于其增加果实库强;两品种蔗糖磷酸合成酶(SPS)活性变化差异不大,说明SPS不是蔗糖积累的关键酶;‘马坑22’蔗糖合成酶(SuSy)合成方向活性在花后144~186d增幅显著高于‘檀头23’,说明SuSy为果实蔗糖积累的关键酶。(3)‘马坑22’蔗糖快速积累主要依靠SuSy合成方向活性变化促进蔗糖合成,‘檀头23’蔗糖快速积累主要依靠SuSy分解方向活性变化促进蔗糖直接进入果实。     

不同时期套袋对幸水梨果实品质、石细胞发育及其相关酶活性变化的影响

以幸水梨为试材分不同时期进行套袋处理,采集各个发育时期的果实,对其果实品质、石细胞团的密度、大小、含量及几种相关酶活性进行了分析.结果表明:(1)不同时期套袋后,幸水梨的硬度、可滴定酸含量均比对照显著增加,可溶性固形物、可溶性总糖含量以及单果重均有不同程度的降低.(2)不同时期套袋果的石细胞含量显著低于对照;与对照果相比套袋果果实表面光洁,果点小而稀,外观品质明显改善.(3)果实石细胞团的密度在幼果期较高,随着果实的发育膨大,密度逐渐减小,成熟前1个月左右基本稳定,石细胞团的纵横径随果实发育先逐渐增大而后减小,石细胞含量也表现出先增加后减少的趋势,在花后第49 d达到最大值.(4)果实内苯丙氨酸解氨酶在果实发育初期的活性较高,随着果实发育逐渐降低;多酚氧化酶活性变化与苯丙氨酸解氨酶相似;过氧化物酶活性随果实的生长呈现先上升后下降的趋势,其活性峰值在盛花后第28 d,其后缓慢下降.(5)不同时期套袋处理后,苯丙氨酸解氨酶、过氧化物酶以及多酚氧化酶活性整体上均比对照降低,与石细胞含量呈正相关关系.     

宁夏枸杞果实糖积累和蔗糖代谢相关酶活性的关系

通过对枸杞果实发育过程中果实生长模式、蔗糖、果糖、葡萄糖和淀粉含量及糖代谢相关酶活性的测定,研究了宁夏枸杞果实生长发育过程中糖的代谢积累与相关酶活性的关系.结果表明:(1)宁夏枸杞果实发育呈双S"曲线,果实主要以积累己糖为主.(2)蔗糖磷酸合成酶(SPS)活性在果实发育初期处于下降的趋势,在花后19d开始上升,果实转色后又逐渐下降;蔗糖合成酶(SS)活性总体表现为SS分解方向的活性大于SS合成方向的活性,说明枸杞果实发育过程中,SS的活性主要以分解方向的为主;酸性转化酶(AI)和中性转化酶(NI)的活性随果实发育呈上升趋势,但在果实成熟后期有所下降,且AI和NI活性高于合成酶类的活性,较高的转化酶类活性促进了果实内部己糖的积累.(3)在枸杞果实生长发育中,葡萄糖和果糖含量与AI和NI均呈极显著正相关,而与其它酶不具有相关性.说明AI和NI在宁夏枸杞果实的糖代谢中起着主要的调控作用.     

氮、磷、钾对豫麦50旗叶蔗糖和籽粒淀粉积累的影响

以豫麦50为对象,探讨了氮、磷、钾对小麦旗叶中蔗糖的积累及相关酶活性以及籽粒中淀粉含量和组分的影响.结果表明,施氮可以增加灌浆前期旗叶中的糖含量,施钾则提高了灌浆后期旗叶中的糖含量,而施磷则对旗叶中的糖含量影响不大.施氮、磷、钾均能增加蔗糖合成酶活性,但它们的作用时间不同：施氮活性增加在籽粒灌浆中期,施磷在灌浆前期,而施钾在灌浆前、中期.但氮亦可增加花后24 d的磷酸蔗糖合成酶活性,施磷增加了灌浆前、中期磷酸蔗糖合成酶活性,施钾则增加了灌浆后期旗叶磷酸蔗糖酶活性.施氮、磷、钾都提高了籽粒中总糖含量,增加籽粒中淀粉含量,其中施钾效果最为明显.施磷提高籽粒中直链淀粉的积累,而施钾则显著提高了籽粒中支链淀粉的含量.     

新疆核桃WJ-CAD和ZJ-CAD基因克隆及时空表达表达分析

肉桂醇脱氢酶(CAD)是木质素生物合成中最后一步反应酶。本研究采用RT-PCR技术克隆露仁核桃WJ-CAD基因和硬壳完整核桃ZJ-CAD基因,研究CAD基因在硬壳完整和露仁核桃内果皮中发育过程中的表达特性。WJ-CAD基因cDNA序列含有788 bp ORF,编码258个氨基酸,分子量84.57 kD,理论等电点5.05;ZJ-CAD基因cDNA序列含有666 bp ORF,编码332个氨基酸,分子量81.93 kD,理论等电点5.09;对所编码的蛋白预测分析表明均属于FrmA超基因家族;实时荧光定量PCR结果表明,WJ-CAD基因的相对表达量整体呈现下降-上升-下降的趋势,ZJ-CAD基因的相对表达量整体均呈现上升-下降-上升-下降的趋势,2个基因均在花后65 d表达量达到最高峰,其后期表达量均较低,CAD基因在"温138"核桃内果皮中的表达量远低于同期"纸皮"的表达量。推测"温138"核桃露仁现象由于花后65 d后木质素合成量降低,导致缺乏木质素的积累或影响木质素单体的组成,从而造成内果皮发育不完整(露仁),初步证明CAD基因参与调控木质素合成,露仁现象可能由于硬核期缺乏木质素的积累。     

The Physiological and Molecular Responses of Exogenous Selenium to Selenium Content and Fruit Quality in Walnut

To study the effect of exogenous selenium on fruit quality in walnut (Juglans regia L.), 8-year-old walnut (Qingxiang) was taken as the research object. In the fruit expansion stage, 300 mg/L of sodium selenate, yeast selenium and sodium selenite solutions were applied on the leaf of walnut, and the selenium levels in leaves, pericarp and kernel were determined at the ripening stage. The fruit quality, mineral nutrient content, antioxidant enzyme activity, and related genes’ expression were analyzed. The results showed that the three exogenous selenium increased the selenium levels in leaves, pericarp and kernel of walnut. They also significantly increased fruit and kernel weights, and kernel linoleic acid, but markedly decreased kernel crude fat and saturated fatty acid. Selenium spraying promoted the absorption of mineral nutrients such as potassium and zinc, but inhibited the absorption of calcium, and had no significant effect on iron and magnesium in the kernel. Three exogenous selenium increased the activities of superoxide dismutase (SOD), peroxidase (POD), ascorbate peroxidase (APX), and catalase (CAT) significantly in the kernel. Except for sodium selenate treatment significantly reduced malondialdehyde (MDA) content in the kernel, the other two selenium sources treatments had no significant effect on MDA and hydrogen peroxide (H₂O₂) levels. They also increased the expression of JrCu/Zn-SOD, Jr2-Cys POD, JrCyt-APX and JrCAT in kernel, to different extents. These implies that, in the walnut fruit enlargement period, the foliar spraying selenium could increase the selenium content of walnut, affect the mineral nutrient absorption, improve the antioxidant capacity and related genes’ expression, and reduce the degree of peroxide, and then improve the quality of fruit. Furthermore, yeast selenium showed the comprehensive effect of the best.     

Regulation of PDH in human arm and leg muscles at rest and during intense exercise

To test the hypothesis that pyruvate dehydrogenase (PDH) is differentially regulated in specific human muscles, regulation of PDH was examined in triceps, deltoid, and vastus lateralis at rest and during intense exercise. To elicit considerable glycogen use, subjects performed 30 min of exhaustive arm cycling on two occasions and leg cycling exercise on a third day. Muscle biopsies were obtained from deltoid or triceps on the arm exercise days and from vastus lateralis on the leg cycling day. Resting PDH protein content and phosphorylation on PDH-E1 alpha sites 1 and 2 were higher (P < or = 0.05) in vastus lateralis than in triceps and deltoid as was the activity of oxidative enzymes. Net muscle glycogen utilization was similar in vastus lateralis and triceps ( approximately 50%) but less in deltoid (likely reflecting less recruitment of deltoid), while muscle lactate accumulation was approximately 55% higher (P < or = 0.05) in triceps than vastus lateralis. Exercise induced (P < or = 0.05) dephosphorylation of both PDH-E1 alpha site 1 and site 2 in all three muscles, but it was more pronounced at PDH-E1 alpha site 1 in triceps than in vastus lateralis (P < or = 0.05). The increase in activity of the active form of PDH (PDHa) after 10 min of exercise was more marked in vastus lateralis ( approximately 246%) than in triceps ( approximately 160%), but when it was related to total PDH-E1 alpha protein content, no difference was evident. In conclusion, PDH protein content seems to be related to metabolic enzyme profile, rather than myosin heavy chain composition, and less PDH capacity in triceps is a likely contributing factor to higher lactate accumulation in triceps than in vastus lateralis.     

Effect of dietary fat on whole body fatty acid synthesis in weanling rats

The effect of dietary fat on body composition, whole body lipogenesis, and enzyme activity was measured in rats over the first 16 weeks post-weaning. Rats were fed either a low fat (5% w/w fat) or high fat (20% w/w fat) diet for the first 4 weeks. After this time all rats were fed the low fat diet. The results showed no significant effect of diet on the rate of fat synthesis over the first 8 weeks of the experiment. However, the activities of the enzymes of fatty acid synthesis [glucose 6-phosphate dehydrogenase, malic enzyme, adenosine triphosphate-citrate lyase, acetyl-coenzyme A carboxylase (ACCX), fatty acid synthetase] were dependent on the age and dietary status of the animals. The exact pattern depended on the specific enzyme and the tissue source. No significant differences in pyruvate dehydrogenase (PDH) activity were observed. Mathematical analysis of the enzyme activities suggested that ACCX and PDH were the most likely sites of fat synthesis regulation. In addition, an examination of body composition and overall weight retention showed that the "weight increasing" effect of a high fat diet could be completely reversed by subsequent feeding of a low fat diet. However, the reversal required an additional 12 weeks. Interestingly, at this time the rats switched from a high fat to a low fat diet had a lower body weight and lower body fat content than rats fed a low fat diet throughout the course of the experiment.     

欧洲榛子贮藏及萌发生理特性研究

对欧洲榛子(Corylus avellanaL.)在沙藏、萌发、幼苗生长阶段的种仁养分和保护酶活性动态变化特点进行了研究,结果表明:种子萌发后107 d营养耗尽;沙藏过程中种仁的干重、粗脂肪含量略下降,萌发后下降迅速且与时间呈线性负相关;可溶性糖在沙藏至萌发后12 d内下降而后上升,萌发24 d后上升迅速,72 d后进入高峰期;淀粉含量在沙藏过程中下降,萌发后上升并在萌发48 d取得11.12%的峰值后下降;游离氨基酸总量在沙藏期间下降,萌发前12 d开始升高,在萌发36~48 d达155.31 mg/100 g的高峰后降低;可溶性蛋白50.87 mg/g的峰值出现在萌发前12 d;SOD在萌发前12 d至萌发后12 d、CAT由萌发起24 d内、POD在萌发36 d后分别出现活性高峰,对种仁营养按测定时间进行的聚类分析结果与依据幼苗干重划分的幼苗生长阶段相吻合。     

钾对小麦旗叶蔗糖和籽粒淀粉积累的影响

 利用冬小麦品种‘鲁麦22’(Triticum aestivum cv. `Lumai 22’)在大田条件下研究了钾素对小麦旗叶蔗糖和籽粒淀粉积累及其有关酶活性的影响。结果表明,钾素有利于提高旗叶光合速率,增强开花后旗叶磷酸蔗糖合成酶活性,提高旗叶中蔗糖的含量;从而提高了灌浆期间籽粒中蔗糖的供应,增强了籽粒中蔗糖合成酶和腺苷二磷酸葡萄糖焦磷酸化酶的活性,加速了淀粉积累速率,提高了粒重和产量。     

Mechanisms of glycolytic control during hibernation in the ground squirrel Spermophilus lateralis

Summary The mechanisms of glycolytic rate control during hibernation in the ground squirrel Spermophilus lateralis were investigated in four tissues: heart, liver, kidney, and leg muscle. Overall glycogen phosphorylase activity decreased significantly in liver and kidney to give 50% or 75% of the activity found in the corresponding euthermic organs, respectively. The concentration of fructose-2,6-bisphosphate (F-2,6-P₂) decreased significantly in heart and leg muscle during hibernation to 50% and 80% of euthermic tissue concentrations, respectively, but remained constant in liver and kidney. The overall activity of pyruvate dehydrogenase (PDH) in heart and kidney from hibernators was only 4% of the corresponding euthermic values. Measurements of phosphofructokinase (PFK) and pyruvate kinase (PK) kinetic parameters in euthermic and hibernating animals showed that heart and skeletal muscle had typical rabbit skeletal M-type PFK and M₁-type PK. Liver and kidney PFK were similar to the L-type enzyme from rabbit liver, whereas liver and kidney PK were similar to the M₂ isozyme found primarily in rabbit kidney. The kinetic parameters of PFK and PK from euthermic vs hibernating animals were not statistically different. These data indicate that tissue-specific phosphorylation of glycogen phosphorylase and PDH, as well as changes in the concentration of F-2,6-P₂ may be part of a general mechanism to coordinate glycolytic rate reduction in hibernating S. lateralis.Abbreviations ADP adenosine diphosphate - AMP adenosine monophosphate - ATP adenonine triphoshate - EDTA ethylenediaminetetra-acetic acid - EGTA ethylene glycol tetra-acetic acid - F-6-P fructose 6-phosphate - F-1,6-P₂ fructose 1,6-bisphosphate - F-2,6-P₂ fructose-2,6-bisphosphate - K _a activation coefficient - I₅₀ concentration of inhibitor which reduces control activity by 50% - PDH pyruvate dehydrogenase - PEP phosphoenolpyruvate - PFK 6-phosphofructo-1-kinase - PK pyruvate kinase     

多效唑对不同品质类型花生产量、品质及相关酶活性的影响

选用高蛋白品种KB008（KB008）、高脂肪品种花17(H17)和高油酸/亚油酸（O/L）品种农大818（818）,在大田栽培条件下,研究了盛花后期叶面喷施多效唑(PBZ)对不同品质类型花生产量、品质及相关碳、氮代谢酶活性的影响.结果表明：喷施PBZ显著增加了3种品质类型花生荚果产量,原因是增加了单株结果数,降低了千克果数而提高了双仁果率.喷施PBZ不同程度地提高了3种类型花生籽仁脂肪和可溶性糖含量,降低了蛋白质含量,显著增加了高脂肪品种H17的O/L值.PBZ使高O/L值品种818的脂肪含量增加显著,同时其蛋白质含量显著降低,而对其他两品种的蛋白质和脂肪含量影响较小.喷施PBZ均降低了3种类型花生结荚期叶片硝酸还原酶（NR）活性及结荚期和饱果期叶片谷氨酰胺合成酶和谷氨酸脱氢酶活性,818的3种酶活性降低幅度最大,KB008和H17的酶活性降幅较小;喷施PBZ均降低了3种类型花生结荚期和饱果期叶片谷草转氨酶和谷丙转氨酶活性.说明氮代谢酶活性的降低是喷施PBZ降低3种类型花生籽仁蛋白质含量的主要原因.喷施PBZ均提高了3品种结荚期和饱果期叶片蔗糖合成酶和磷酸蔗糖合成酶活性,其中显著提高了818的2种酶活性,而对KB008和H17的活性提高不显著;喷施PBZ提高了3品种结荚期和饱果期的磷酸烯醇式丙酮酸羧化酶和1,5-二磷酸核酮糖羧化酶活性,其中对818在结荚期的活性提高最显著,对H17活性提高较小.碳代谢酶活性的增强是喷施PBZ提高花生籽仁脂肪含量的生理基础.