喜马拉雅地区红矛猛蚁Myopopone castanea (Smith)记述

矛猛蚁属Myopopone Roger分布于东洋界和马来西亚界,目前为止该属仅知1种:红矛猛蚁M.castanea (Smith).首次在中国西藏记录到红矛猛蚁的分布,并系统记述了矛猛蚁属的特征、红矛猛蚁工蚁和蚁后的特征,提供了详尽的测量数据和插图.     

Evidence for Exploitative Competition: Comparative Foraging Behavior and Roosting Ecology of Short-Tailed Fruit Bats (Phyllostomidae)

Chestnut short-tailed bats, Carollia castanea , and Seba's short-tailed bats, C. perspicillata (Phyllostomidae), were radio-tracked ( N = 1593 positions) in lowland rain forest at Tiputini Biodiversity Station, Orellana Province, Ecuador. For 11 C . castanea , mean home range was 6.8 ± 2.2 ha, mean core-use area was 1.7 ± 0.8 ha, and mean long axis across home range was 438 ± 106 m. For three C . perspicillata , mean home range was 5.5 ± 1.7 ha, mean core-use area was 1.3 ± 0.6 ha, and mean long axis was 493 ± 172 m. Groups of less than five C. castanea occupied day-roosts in earthen cavities that undercut banks the Tiputini River. Carollia perspicillata used tree hollows and buildings as day-roosts. Interspecific and intraspecific overlap among short-tailed bats occurred in core-use areas associated with clumps of fruiting Piper hispidum (peppers ) and Cecropia sciadophylla . Piper hispidum seeds were present in 80 percent of the fecal samples from C . castanea and 56 percent of samples from C . perspicillata . Carollia perspicillata handled pepper fruits significantly faster than C . castanea ; however, C . castanea commenced foraging before C . perspicillata emerged from day-roosts. Evidence for exploitative competition between C . castanea and C . perspicillata is suggested by our observations that 95 percent of ripe P . hispidum fruits available at sunset disappear before sunrise ( N = 74 marked fruits). Piper hispidum plants produced zero to 12 ripe infructescences per plant each night during peak production. Few ripe infructescences of P . hispidum were available during the dry season; however, ripe infructescences of C . sciadophylla , remained abundant.     

Characterization of a highly repeated DNA sequence (SC1) from the arbuscular mycorrhizal fungus Scutellospora castanea and its detection in planta.

A highly repeated DNA sequence from the genome of an arbuscular mycorrhizal fungus has been isolated and characterized. This 1,202-bp sequence (SC1) represents about 0.24% of the Scutellospora castanea genome, estimated to be 1 pg by flow cytometry. The sequence was shown to be a Scutellospora-specific probe in Southern blots and dot blot hybridizations. After complete sequencing of SC1, PCR primers were generated and used to amplify a 907-bp fragment from spores of S. castanea or from colonized Allium porrum roots. No amplification products were obtained with DNA from either spores or mycorrhizal root of other species of arbuscular mycorrhizal fungi. These primers were sufficiently specific for unequivocal detection of S. castanea in planta.     

Susceptibility of four species of turfgrass-infesting scarabs (Coleoptera: Scarabaeidae) to Bacillus thuringiensis serovar japonensis strain Buibui

Bacillus thuringiensis serovar japonensis strain Buibui experimental formulations containing Cry8Cal delta-endotoxin were evaluated in the field for the control of oriental beetle, Anomala orientalis Waterhouse; Japanese beetle, Popillia japonica Newman; Asiatic garden beetle, Maladera castanea (Arrow); and European chafer, Rhizotrogus majalis (Razoumowsky) larvae infesting turfgrasses. In 17 fall applications, control of A. orientalis and P. japonica was achieved at rates as low as 100 g of toxin per ha; however, rates of 301 or 593 g/ha were consistently more effective. A. orientalis larvae were the most susceptible followed by P. japonica, R. majalis, and M. castanea. Two spring applications of 120 g of toxin per ha were not effective against oriental or Japanese beetles.     

Intraspecific ITS polymorphism in Scutellospora castanea (Glomales, Zygomycota) is structured within multinucleate spores.

Polymorphism of the internal transcribed spacers (ITS) of the ribosomal DNA in Scutellospora castanea (Glomales, Zygomycota) and its organization among spores were evaluated. Polymerase chain reaction (PCR) amplification with ITS1/ITS4 primers yielded several fragments of different lengths, even from single spores. Fragments produced from multisporal DNA were cloned and grouped into 6 ITS types by PCR-RFLP and sequence analysis. Five type-specific primers were designed. Spores were then analyzed by PCR and amplification profiles revealed that they were qualitatively different one from another due to the presence or absence of some ITS types. Intrasporal segregation of ITS variant length types was also shown, by PCR experiments, utilizing diluted fractions of nuclear suspensions from single spores. The results demonstrate the mainly multikaryotic condition of the spores of S. castanea.     

高黎贡山齿甲属六新种(鞘翅目,拟步甲科)

描述中国云南高黎贡山齿甲属Uloma Latreille 6新种,模式标本保存于河北大学博物馆.     

Testa sculpture in the genus Frailea (Cactaceae)

The primary sculpture of the seed surface in the genus Frailea (Cactaceae) is characterized by a i flat outer periclinal wall and the presence of an usually eccentric papilla. Papilla morphology differs mainly in size and breadth:length ratio. Seeds of different species (groups) are characterized by special patterns in the arrangement of short and long papillae on the surface, thus providing a useful tool for taxonomic studies in Frailea. The seeds of Frailea castanea possess branched papillae, a character restricted in this genus to this species. Moreover, such papilla morphology seems to be unique for the seed epidermis in spermatophytes. The possible function of the papillae is discussed.     

Identification and isolation of two ascomycete fungi from spores of the arbuscular mycorrhizal fungus Scutellospora castanea

Two filamentous fungi with different phenotypes were isolated from crushed healthy spores or perforated dead spores of the arbuscular mycorrhizal fungus (AMF) Scutellospora castanea. Based on comparative sequence analysis of 5.8S ribosomal DNA and internal transcribed spacer fragments, one isolate, obtained from perforated dead spores only, was assigned to the genus Nectria, and the second, obtained from both healthy and dead spores, was assigned to Leptosphaeria, a genus that also contains pathogens of plants in the Brassicaceae. PCR and randomly amplified polymorphic DNA-PCR analyses, however, did not indicate similarities between pathogens and the isolate. The presence of the two isolates in both healthy spores and perforated dead spores of S. castanea was finally confirmed by transmission electron microscopy by using distinctive characteristics of the isolates and S. castanea. The role of this fungus in S. castanea spores remains unclear, but the results serve as a strong warning that sequences obtained from apparently healthy AMF spores cannot be presumed to be of glomalean origin and that this could present problems for studies on AMF genes.     

Histological study of Goniodoris castanea Alder and Hancock, 1845 (Nudibranchia, Doridoidea, Goniodorididae)

The major organ systems of Goniodoris castanea were investigated by histological means, with an emphasis on those structures that are difficult to see by dissection. The species is characterized by some peculiar features that are unique or seldom within the Nudibranchia, such as the complete absence of specialized vacuolated cells, the presence of globular salivary glands, the presence of cuticular structures in the proximal intestine, a muscular sphincter around the distal vaginal duct, and the position of the blood gland closer to the pericardium than to the nervous system. Some of these characters are discussed in a phylogenetic context, although a thorough phylogenetic analysis is preliminary, due to lack of knowledge of probably related species.     

Phylogenetic analysis of a dataset of fungal 5.8S rDNA sequences shows that highly divergent copies of internal transcribed spacers reported from Scutellospora castanea are of ascomycete origin

Using a dataset comprising 5.8S rDNA sequences from a wide range of fungi, we show that some sequences reported recently from the arbuscular mycorrhizal (AM) fungus Scutellospora castanea most likely originate from Ascomycetes. Other ITS and 5.8S sequences which were previously reported are confirmed as being clearly of mycorrhizal origin and are variable within one isolate of S. castanea. However, these results mean that previous conclusions which were drawn regarding the heterokaryotic status of AM fungal spores remain unproven. We provide an enlarged 5.8S rDNA dataset that can be used to check ITS sequences for conflicts with well-established phylogenies of the organisms that they were obtained from.     

Structural color production by constructive reflection from ordered collagen arrays in a bird (Philepitta castanea: Eurylaimidae)

Ordered hexagonal arrays of parallel collagen fibers produce the brilliant green structural color of the fleshy, supraorbital caruncles of male Velvet Asity (Philepitta castanea; Aves: Eurylaimidae). The collagen arrays are organized in larger macrofibrils that are packed irregularly within cone-shaped papillae that cover the surface of the caruncle. The color of the caruncle conforms closely to the wavelengths predicted by applying Bragg's Law of constructive reflection to measurements of the size and spatial organization of the collagen arrays. These observations constitute a novel mechanism of structural color production in animals. These collagen arrays are convergently similar to the smaller, highly structured collagen arrays in the mammalian cornea, which exploit the same physical mechanism to produce optical transparency. © 1994 Wiley-Liss, Inc.     

Comparative phylogeography of short-tailed bats (Carollia: Phyllostomidae)

This is the first study of comparative phylogeography involving closely related species of Neotropical bats of the family Phyllostomidae. We compared patterns of geographical variation within the five species of fruit-eating bats currently recognized in the genus Carollia using the complete mitochondrial cytochrome-b gene. Our results suggest that the combined effect of the uplift of the Andes and the Panamanian land bridge has been as important for bats as for terrestrial mammals in shaping present-day biodiversity in the New World tropics. Species in this genus can be arranged in two highly supported clades, with a deep subdivision within each that corresponds well to differences across the Andes. We found three congruent phylogeographical patterns across species in this genus. First, the closer relationship between samples from western Ecuador and those from Central America, compared with populations east of the Andes in C. brevicauda, C. castanea and C. perspicillata. Second, the likelihood of a similar timing in South America for the arrival and diversification of C. brevicauda and C. perspicillata from their Central America ancestors. Third, the expansion of C. perspicillata and C. sowelli into northwestern Central America in the relatively recent past. Using a molecular clock, with rates ranging from 2.3 to 5% per 10(6) years, diversification within Carollia would have occurred over the last 1-4.5 Myr. These estimates agree well with the last rise of the Northern Andes and the Panama isthmus.     

Biological and chemical control of the Asiatic garden beetle, Maladera castanea (Coleoptera: Scarabaeidae)

The efficacy of chemical and biological control agents against larvae of the Asiatic garden beetle, Maladera castanea (Arrow), in turfgrass under laboratory, greenhouse, and field conditions were determined. In field trials where insecticides were applied preventively against eggs and young larvae, the molt-accelerating compound halofenozide and the neonicotinoids imidacloprid and thiamethoxam were ineffective, whereas another neonicotinoid, clothianidin, provided 62-93% control. In greenhouse experiments against third instars in pots, the carbamate insecticide carbaryl was ineffective, whereas the organophosphate trichlorfon provided 71-83% control. In laboratory, greenhouse, and field experiments, the entomopathogenic nematode Heterorhabditis bacteriophora Poinar and Steinernema glaseri Steiner (not tested in the field) were ineffective against third instars, whereas S. scarabaei Stock & Koppenh?fer provided excellent control. In microplot field experiments at a rate of 2.5 x 10(9) infective juveniles per ha, H. bacteriophora provided 12-33% control and S. scarabaei 71-86% control. Combinations of S. scarabaei and imidacloprid did not provide more control of third instars compared with S. scarabaei alone.     

A tangled tale of two teal: population history of the grey Anas gracilis and chestnut teal A. castanea of Australia

Two Australian species of teal (Anseriformes: Anatidae: Anas ), the grey teal Anas gracilis and the chestnut teal A. castanea , are remarkable for the zero or near-zero divergence recorded between them in earlier surveys of mitochondrial DNA (mtDNA) diversity. We confirmed this result through wider geographical and population sampling as well as nucleotide sampling in the more rapidly evolving mtDNA control region. Any data set where two species share polymorphism as is the case here can be explained by a model of gene flow through hybridization on one hand or by incomplete lineage sorting on the other hand. Ideally, analysis of such shared polymorphism would simultaneously estimate the likelihood of both phenomena. To do this, we used the underlying principle of the IMa package to explore ramifications to understanding population histories of A. gracilis and A. castanea . We cannot reject that hybridization occurs between the two species but an equally or more plausible finding for their nearly zero divergence is incomplete sorting following very recent divergence between the two, probably in the mid-late Pleistocene. Our data add to studies that explore intermediate stages in the evolution of reciprocal monophyly and paraphyletic or polyphyletic relationships in mtDNA diversity among widespread Australian birds.     

Characterization of root colonization profiles by a microcosm community of arbuscular mycorrhizal fungi using 25S rDNA-targeted nested PCR

The aim of the present work was to study colonization patterns in roots by different arbuscular mycorrhizal fungi developing from a mixed community in soil. As different fungi cannot be distinguished with certainty in planta on the basis of fungal structures, taxon-discriminating molecular probes were developed. The 5' end of the large ribosomal subunit containing the variable domains D1 and D2 was amplified by PCR from Glomus mosseae (BEG12), G. intraradices (LPA8), Gigaspora rosea (BEG9) and Scutellospora castanea (BEG1) using newly designed eukaryote-specific primers. Sequences of the amplification products showed high interspecies variability and PCR taxon-discriminating primers were designed to distinguish between each of these four fungi. A nested PCR, using universal eukaryotic primers for the first amplification and taxon-discriminating primers for the second, was performed on individual trypan blue-stained mycorrhizal root fragments of onion and leek, and root colonization by four fungi inoculated together in a microcosm experiment was estimated. More than one fungus was detected in the majority of root fragments and all four fungi frequently co-existed within the same root fragment. Root colonization by G. mosseae and G. intraradices was similar from individual and mixed inoculum, whilst the frequency of S. castanea and Gig. rosea increased in the presence of the two Glomus species, suggesting that synergistic interactions may exist between some arbuscular mycorrhizal fungi.     

Biology of the New Zealand genus Neolimnia (Diptera: Sciomyzidae)

Abstract

Data are presented on the life cycles of eight species of Neolimnia, an endemic New Zealand genus of snail-killing flies. Habitats, geographical distributions, biological features of adults and immature stages, including adult and larval behaviour and feeding habits, and phenology are discussed. Larvae of subgenus Pseudolimnia live in aquatic environments; those of N. (P.) repo, N. (P.) sigma, and N. (P.) ura prey on aquatic pulmonate snails, but those of N. (P.) tranquilla prey on aquatic prosobranch snails. Larvae of Neolimnia (Neolimnia) castanea, N. (N.) irrorata, N. (N.) obscura, and N. (N.) striata live in terrestrial environments, and apparently prey overtly on terrestrial snails in nature, but will also attack aquatic snails in laboratory rearings.     

Transmission of stridulatory signals of the burrower bugs, Scaptocoris castanea and Scaptocoris carvalhoi (Heteroptera: Cydnidae) through the soil and soybean

Abstract.  Males and females of the burrower bug species Scaptocoris castanea Perty and Scaptocoris carvalhoi Becker emit stridulatory signals when on the roots of soybean. The substrate-borne components of the signal can be recorded on the plant but not on the surrounding soil surface. The stridulatory apparatus is composed of the tergal plectrum (lima) and the stridulitrum (stridulatory vein) on the underside of the hind wings. The male plectrum has one ridge and the female lima has 13 ridges. Stridulitra of different species differ in the length and in the number of teeth. Rubbing of plectrum (lima) ridges over the stridulitrum in one or both directions produces pulse trains. The velocity of signals that are recorded less than 0.5 cm from the bug is below 0.013 mm s⁻¹ on the soil and below 0.066 mm s⁻¹ on the leaf surface. Broadband spectra have a dominant frequency of less than 1 kHz and subdominant peaks extending up to 7 kHz. The dominant frequency of the stridulatory signal transmitted through a plant decreases together with the proportion of its higher frequency spectral components. Signals are attenuated for 3–9 dB cm⁻¹ when transmitted through the soil or soybean leaf and for approximately 1 dB cm⁻¹ when transmitted through soybean stem.