豆科长柄山蚂蝗属一新组合

Ohashi和Mill提出长柄山蚂蝗属的正确学名是Hylodesmum H.Ohashi&R.R.Mill,用以取代由来已久的属名Podocarpium（Benth.）Y.C.Yang&P.H.Huang。本文根据该属属名的变化,提出并确定澜沧长柄山蚂蝗的新组合名称为Hylodesmum lancangense（Y.Y.Qian X.Y.Zhu&H.Ohashi。     

中国豆科和樟科几种植物学名的订正

在编写《Flora of China》过程中,根据标本和文献的查阅,对国产豆科和樟科几种植物学名进行修订。本文记述5个新组合、4个新异名和一个恢复的种名。5个新组合是:垂果山蚂蝗Desmodium strigilosum var.pendenticarpum(C.Z.Gao Q.R.Lai) P.H.Huang,心叶山蚂蝗Desmodium flexuosum var.cordifoliolatum(P.C.Li) P.H.Huang,密毛长柄山蚂蝗Podocarpium densum(C.Chen et X.J.Cui)P.H.Huang,东北长柄山蚂蝗Podocarpium podocarpum var.mandshuricum(Maxim.) P.H.Huang和尖叶长柄山蚂蝗Podocarpium podocarpum var.japon-icum(Matsum.)P.H.Huang.4个新异名如下:李氏木姜子Litsea lii C.E.Chang及其变种能汉木姜子var.nunkao-tahangensis(Liao) Liao作为竹叶木姜子L.pseudoelongata Liou Ho的新异名,竹头角木姜子L.akosensis var.chitouchiaoensis Liao作为台湾木姜子L.hayatae Kanehira的新异名和密毛山蚂蝗Desmodium densum (C.Chen et X.J.Cui)H.Ohashi作为密毛长柄山蚂蝗Podocarpium densum (C.Chen et X.J.Cui)P.H.Huang的新异名。由于Litsea pedicellata一名被占用,恢复Litsea taiwaniana Kamikoti作为黄肉树合法的种名。     

长柄山蚂蝗属三个种的核型分析及山蚂蝗属两个种的染色体数

长柄山蚂蝗属Podocarpium(Benth.)Yang et Huang属蝶形花科,间断分布于东亚和中北美洲,广布种分布到非洲和大洋洲。北美有4种,亚洲约有14种.我国约有该属植物11种,以西南和长江以南地区分布种类最多。国外已有人做过本属一半以上的种的染色体记数和核型。本文对本属的三个种.长柄山蚂蝗P.podocarpum(DC.)Yang et Huang,大苞长柄山蚂蝗P.williamsii(Pamp.)Yang et Huang和云南长柄山吗蝗P.du-clouxii(Pamp.)Yang et Huang进行了染色体的记数和核型描述,其中后两个种的工作属于首次。此外,本文还首次报道了山蚂蝗属两个种:Des-modium caudatum(Thunb.)DC.和D.yunnanense Franch.的染色体数目。     

贵州种子植物分布新记录

报道了贵州分布新记录植物19种4变种。即类叶升麻(Actaea asiatica Hara)、长距翠雀花(Delphinium tenii Lévl.)、白蓝翠雀花(Delphinium albocoeruleum Maxim.)、云南铁线莲(Clematis yunnanensis Franch.)、女萎(Clematis apiifolia var. apiifolia DC.)、根叶漆姑草(Sagina maxima A. Gray)、小酸模(Rumex acetosella Linn.)、皱果赤瓟(Thladiantha henryi Hemls.)、团叶杜鹃(Rhododendron orbiculare Decne)、大理鹿蹄草(Pyrola forrestiana H. Andr)、蛇莓委陵菜(Potentilla centigrana Maxim.)、腺梗蔷薇(Rosa filipes Rehd. et Wils)、疏花长柄山蚂蝗(Hylodesmum laxum (DC.) H. Ohashi et R. R. Mill)、侧序长柄山蚂蝗(Hylodesmum laterale (Schindl.) H. Ohashi et R. R. Mill)、茳芒香豌豆(Lathyrus davidii Hance.)、牧场黄蓍(Astragalus pastoricus Tsai et Yu.)、白花酢浆草(Oxalis acetosella Linn.)、屏边三七(Panax stipuleanatus C. T. Tsai et K. M. Meng)、腺毛粉条儿菜(Aletris glandulifera Bur.)、蜜腺白叶莓(变种)(Rubus innominatus var. aralioides (Hance) Yü et Lu)、绢毛匍匐委陵菜(变种)(Potentilla reptans L. var. sericophylla Franch.)、狭萼鬼吹萧(变种)(Leycesteria formosa Wall. var. slenosepala Rehd.)、金黄花滇百合(变种)(Lilium bakerianum var. aureum Grove et Cotton)。     

云南长柄山蚂蝗属一新种

发表长柄山蚂蝗属一新种。     

中国蒿属植物新资料(Ⅳ)

本文补充了中国蒿属植物新资料,即太白山蒿A. taibaishanensis Y. R. Ling & C. J. Humohries, sp. nov.;吉隆蒿A. jilongensis Y. R. Ling & C. J. Humphries, sp. nov.;昌都蒿A. orientali-xizangensis Y. R. Ling & C. J. Humphries, sp. nov.;贡山蒿A. gongshanensis Y. R. Ling & C. J. Humphries, sp. nov.。     

蒿属植物一新名

<正> 细裂叶蒿 Artemisia austro-himalayana A.R.Ling & H.S.Puri,nom.nov.—A.ten ifo iasensu Y.R.Ling & H.S.Puri in Guihaia 5(1): 2.1985,non Adam.ex DC.     

福建省豆科植物根瘤菌资源的初步调查

对福建省境内(除东北部少数地区外)的29个县(市)豆科植物根瘤菌资源进行了调查,共采集到36属63种豆科植物的根瘤样品278份,在豆科栽培作物方面有一定代表性.采集到的根瘤形态多样,大小多在1-10 mm之间,以淡黄和淡红色居多.对32种51份根瘤样品用乙炔还原法进行了固氮酶活性测定,不同种和同一种植物在不同生境下的固氮酶活性均有差异,多数根瘤样品的固氮酶活性在1-24 μmol C2H4g-1 fresh nodule h-1之间,其中尖叶长柄山蚂蝗(Podocarpium podocarpum var.oxyphyllum)的固氮酶活性相对最高,达87.29 u mol C2H4 g-1 fresh nodule h-1.从采集到的根瘤中经分离纯化获得了228株根瘤菌,其中155株根瘤菌进行了原寄主回接试验,有138株结瘤,回接成功率为89.0%.调查结果显示福建省有丰富的豆科固氮及其根瘤菌资源.     

疏花长柄山蚂蝗复合体(豆科)的修订

通过文献、标本研究,结合野外考察及数据统计分析,确认疏花长柄山蚂蝗复合体包括4种1亚种,讨论了各分类群的重要性状,并给出了新的检索表。将湘西长柄山蚂蝗(Hylodesmum laxum subsp.falfolium)处理为原亚种的新异名;订正了《中国植物志》和《Flora of China》等著作中该类群的几个错误的地理分布,记载了我国几个省区的分布新记录,并给出了凭证标本。     

大孢卧孔菌一新组合

本文将刺孢孔菌Pachykytospora中的P. major G. Y. Zheng & Z. S. Bi组合为Megasporoporia major (G.Y. Zheng & Z. S. Bi) Y. C. Dai & T. H. Li,并根据模式标本对该种进行了详细描述。     

A new synonym of Hylodesmum podocarpum ssp. podocarpum (Leguminosae)

Close examination of the type material of Podocarpium lancangense Y. Y. Qian has shown that this species is indistinguishable from Hylodesmum podocarpum (DC.) H.　Ohashi & R. R. Mill ssp. podocarpum, and thus is reduced to synonymy herein.     

Antiosteoporotic flavonoids from Podocarpium podocarpum

A novel bi-isoflavonoid, podocarnone (1), together with five known flavonoids, namely genistein (2), afzelin (3), astragalin (4), luteolin (5) and pratensein (6), were isolated from the whole plants of Podocarpium podocarpum (DC.) Yang and Huang for the first time. The structure of podocarnone (1) was elucidated by detailed spectroscopic analyses, including 1D and 2D NMR techniques. All the isolated compounds were evaluated for their proliferative effects on osteoblasts derived from neonatal rat calvaria and inhibitory effects on multinucleated osteoclasts from rat marrow cells so as to explore the antiosteoporotic activity of these components. Podocarnone (1) exhibited potent stimulatory effects on osteoblastic proliferation and ALP (alkaline phosphatase) activity, and significantly inhibited the activity of osteoclastic TRAP (tartrate-resistant acid phosphatase) in the low concentration range of 10^?12–10^?14 mol/L, which were equivalent to the activity of genistein (2) in the concentration range of 10^?7–10^?9 mol/L. The other five known flavonoids also showed varied degrees of antiosteoporotic activities, and structure–activity relationship analysis revealed the number of phenolic rings contained in these structures maybe responsible for the antiosteoporosis property.     

中国马蓝属(爵床科)新组合和新分类群(英文)

采用广义的马蓝属(Strobilanthes Blume)概念,提出3个新组合:匍匐半插花(S.primulifolia(Nees)Y.F.DengJ.R.I.Wood),直立半插花(S.cumingiana(Forst.)Y.F.DengJ.R.I.Wood)和狭叶马蓝(S.atropurpurea var.stenophylla(C.B.Clarke)Y.F.DengJ.R.I.Wood);描述了8新种:南岭马蓝(Strobilanthes austrosinensis Y.F.DengJ.R.I.Wood)、冯氏马蓝(S.fengiana Y.F.DengJ.R.I.Wood)、陶氏马蓝(S.taoana Y.F.DengJ.R.I.Wood)、启无马蓝(S.wangiana Y.F.DengJ.R.I.Wood)、景东马蓝(S.atroviridis Y.F.DengJ.R.I.Wood)、西畴马蓝(S.rostrata Y.F.DengJ.R.I.Wood)、黄连山马蓝(S.spiciformis Y.F.DengJ.R.I.Wood)和匍匐马蓝(S.procumbens Y.F.DengJ.R.I.Wood)。对南岭马蓝、黄连山马蓝、景东马蓝和匍匐马蓝的花粉形态进行了观察。     

Receptor subtype-specific docking of Asp6.59 with C-terminal arginine residues in Y receptor ligands

Y receptors (YRs) are G protein-coupled receptors whose Y(1)R, Y(2)R, and Y(5)R subtypes preferentially bind neuropeptide Y (NPY) and peptide YY, whereas mammalian Y(4)Rs show a higher affinity for pancreatic polypeptide (PP). Comparison of YR orthologs and paralogs revealed Asp(6.59) to be fully conserved throughout all of the YRs reported so far. By replacing this conserved aspartic acid residue with alanine, asparagine, glutamate, and arginine, we now show that this residue plays a crucial role in binding and signal transduction of NPY/PP at all YRs. Sensitivity to distinct replacements is, however, receptor subtype-specific. Next, we performed a complementary mutagenesis approach to identify the contact site of the ligand. Surprisingly, this conserved residue interacts with two different ligand arginine residues by ionic interactions; although in Y(2)R and Y(5)R, Arg(33) is the binding partner of Asp(6.59), in Y(1)R and Y(4)R, Arg(35) of human PP and NPY interacts with Asp(6.59). Furthermore, Arg(25) of PP and NPY is involved in ligand binding only at Y(2)R and Y(5)R. This suggests significant differences in the docking of YR ligands between Y(1/4)R and Y(2/5)R and provides new insights into the molecular binding mode of peptide agonists at GPCRs. Furthermore, the proposed model of a subtype-specific binding mode is in agreement with the evolution of YRs.     

Inhibiting neuropeptide Y Y1 receptor modulates melanocortin receptor- and NF-κB-mediated feeding behavior in phenylpropanolamine-treated rats

Neuropeptide Y (NPY) and nuclear factor-kappa B (NF-κB) are involved in regulating anorexia elicited by phenylpropanolamine (PPA), a sympathomimetic drug. This study explored whether NPY Y1 receptor (Y1R) is involved in this process, and a potential role for the proopiomelanocortin system was identified. Rats were given PPA once a day for 4 days. Changes in the hypothalamic expression of the NPY, Y1R, NF-κB, and melanocortin receptor 4 (MC4R) levels were assessed and compared. The results indicated that food intake and NPY expression decreased, with the largest reductions observed on Day 2 (approximately 50% and 45%, respectively), whereas NF-κB, MC4R, and Y1R increased, achieving maximums on Day 2 (160%, 200%, and 280%, respectively). To determine the role of Y1R, rats were pretreated with Y1R antisense or a Y1R antagonist via intracerebroventricular injection 1 h before the daily PPA dose. Y1R knockdown and inhibition reduced PPA anorexia and partially restored the normal expression of NPY, MC4R, and NF-κB. The data suggest that hypothalamic Y1R participates in the appetite-suppression from PPA by regulating MC4R and NF-κB. The results of this study increase our understanding of the molecular mechanisms in PPA-induced anorexia.     

Antiosteoporotic activity and constituents of Podocarpium podocarpum

Our study aimed to investigate the antiosteoporotic properties of the ethanol extract of Podocarpium podocarpum (DC.) Yang et Huang (PE) in ovariectomized (OVX) rats and to characterize the active constituents. As a result, PE significantly inhibited the increased urinary Ca excretion and activity of bone resorption markers including tartrate-resistant acid phosphatase (TRAP), deoxypyridinoline crosslinks and cathepsin K in OVX rats, whereas exhibited little effects on the body, uterus and vagina weight. Detailed micro-CT analysis showed that PE notably enhanced bone quality, with increased bone mineral content (BMC), bone volume fraction (BVF), connectivity density (CD), tissue mineral content (TMC), tissue mineral density (TMD) and trabecular number (Tb. N), and decreased trabecular separation (Tb. Sp), in OVX animal. Those findings implied that PE had notable antiosteoporotic effect, especially effective in preventing bone resorption, with little side-effects on reproductive tissue. Further chemical investigation led to the isolation of 17 flavonoids, most of which showed significantly stimulatory effect on osteoblastic proliferation, ALP activity and mineralized nodes formation as well as inhibitory effect on osteoclastic TRAP activity in osteoblastic and osteoclastic cells. Our results indicated that PE, with abundant flavonoids, had remarkable antiosteoporotic activity and therefore can be a promising candidate for the treatment of postmenopausal osteoporosis induced by estrogen deficiency through herbal remedy.     

Neuropeptide Y Y5-receptor activation on breast cancer cells acts as a paracrine system that stimulates VEGF expression and secretion to promote angiogenesis

Accumulating data implicate a pathological role for sympathetic neurotransmitters like neuropeptide Y (NPY) in breast cancer progression. Our group and others reported that NPY promotes proliferation and migration in breast cancer cells, however the angiogenic potential of NPY in breast cancer is unknown. Herein we sought to determine if NPY promotes angiogenesis in vitro by increasing vascular endothelial growth factor (VEGF) expression and release from 4T1 breast cancer cells. Western blot analysis revealed that NPY treatment caused a 52 ± 14% increase in VEGF expression in the 4T1 cells compared to non-treated controls. Using selective NPY Y-receptor agonists (Y1R, Y2R and Y5R) we observed an increase in VEGF expression only when cells were treated with Y5R agonist. Congruently, using selective Y1R, Y2R, or Y5R antagonists, NPY-induced increases in VEGF expression in 4T1 cells were attenuated only under Y5R antagonism. Endothelial tube formation assays were conducted using conditioned media (CM) from NPY treated 4T1 cells. Concentration-dependent increases in number of branch points and complete endothelial networks were observed in HUVEC exposed to NPY CM. CM from Y5R agonist treated 4T1 cells caused similar increases in number of branch points and complete endothelial networks. VEGF concentration was quantified in CM (ELISA) from agonist experiments; we observed a 2-fold and 2.5-fold increase in VEGF release from NPY and Y5R agonist treated 4T1 cells respectively. Overall these data highlight a novel mechanism by which NPY may promote breast cancer progression, and further implicate a pathological role of the NPY Y5R.     

The hybridization of glyceraldehyde 3-phosphate dehydrogenases from rabbit muscle and yeast. Kinetics and thermodynamics of the reaction and isolation of the hybrid

A kinetic and thermodynamic study was made of the formation of the hybrid (R(2)Y(2)) glyceraldehyde 3-phosphate dehydrogenase from the yeast (Y(4)) and rabbit (R(4)) enzymes. The values of the thermodynamic parameters for the equilibrium between R(4), Y(4) and R(2)Y(2) suggest that the R(2)-R(2) and Y(2)-Y(2) interactions are similar. However, the failure to observe the RY(3) and R(3)Y hybrids is interpreted in terms of differences at the interfaces of the R-R and Y-Y interactions (the glyceraldehyde 3-phosphate dehydrogenase molecule being regarded as a dimer of dimers). The kinetics of formation of the R(2)Y(2) hybrid were studied and a model was proposed to account for the results. Best-fit values for the rate constants of the individual steps were evaluated by computer simulation, and the rate-limiting steps were identified as the dissociation of tetramers to dimers. It is proposed that the cleavage plane for dissociation of the tetramers corresponds to the region of low electron density through the centre of the molecule in the X-ray-crystallographic structure for human glyceraldehyde 3-phosphate dehydrogenase (Watson et al., 1972), which is probably the plane containing the Q and R axes in the lobster enzyme (Buehner et al., 1974). The R(2)Y(2) hybrid was isolated in milligram amounts by ion-exchange chromatography and its rate of reversion to the native enzyme was shown to be consistent with the kinetic model proposed from the hybrid-formation experiments.     

On the Difference Between Elachanthemum Y. Ling et Y. R. Ling and Stilpnolepis Krasch.

It is quite unreasonable reducing Elachanthemum Y. Ling et Y. R. Ling into Stilprolepis Krasch. and it is so wrong idea attributing the achenes and cupuliform corolla (as a matter of fact, the cupuliform corolla is originally from the Stilpnolepis Krasch., not from Elachanthemum Y. Ling et Y. R. Ling) of Elachanthemum Y. Ling et Y. R. Ling to “earlier Development” in the paper published in Act. Phytotax. Sin. 23(6): 470-472. 1985. In Elachanthemum Y. Ling et Y. R. Ling, bracts of capitula herbaceous, obviously floccose on the abaxial surface and membranaceous only on the margin, corolla of bisexual florets tubular, achenes oblique, obovoid, and the exine of pollen grains minutespinulate, but in Stilpnolepis Krasch., on the contrary, whole bracts membranaceous, glabrous, corolla of hermaphrodite florets cupuliform or campanulate, achenes long-clavate or fuciform and the exine of pollen grains remarkably spiny.