悬钩子属部分类群的分类订正

根据野外调查和查阅标本的结果, 对悬钩子属部分类群做了修订。将红毛悬钩子(R.pinfaensis Lévl.et Vant.)并入椭圆悬钩子(R.ellipticus Smith), 栽秧泡(R.ellipticus Smithvar.obcordatus (Franch.)Focke)提升为种(R.obcordatus (Franch.)Focke);棱枝细瘦悬钩子(R.macilentus Camb.var.angulatus Delav.)并入细瘦悬钩子(R.macilentus Camb.);毛叶插田泡(R.coreanus Miq.var.tomentosus Card.)并入插田泡(R.coreanus Miq.);狭叶绢毛悬钩子(R.lineatus Reinw.var.angustifolius Hook.)并入绢毛悬钩子(R.lineatus Reinw.)。对针刺悬钩子(R.pungens Camb.)及其各变种做了订正。     

国产悬钩子属绢毛亚组新资料

报道了采自西藏墨脱县悬钩子属绢毛亚组Rubus subsect. Lineati (Focke) Yü et Lu两个中国新纪录种, 即酒红悬钩子R.calophyllus Clarke.和炫丽悬钩子R.splendidissimus Hara.     

悬钩子属2中国新记录种

报道了悬钩子属植物的2个中国新记录种,分别为浅裂悬钩子(Rubus polyadenus Cardot)和滇印悬钩子(R.opulifolius Bertol)。浅裂悬钩子发现于云南省东南部的河口和个旧等地,其与黔桂悬钩子(R.feddei H.Lév.Vaniot)较为相近,区别在于该种枝条、叶柄和花序轴密被浅红色具腺长刺毛,叶柄较短,叶片长卵形或近琴形,顶端急尖或渐尖,萼片三角状披针形,顶端长尾尖。滇印悬钩子新发现于云南省西北部的高黎贡山,其与拟覆盆子(R.idaeopsis Focke)较为相近,区别后者在于其小叶3~5枚,花序极短,不足3cm长,花柄很短,整个花序密集缩短成近头状。     

两种悬钩子属植物在中国的重新发现

报道了蔷薇科两种悬钩子属植物在中国的重新发现,分别为美叶悬钩子(Rubus calophyllus C.B.Clark)和拟针刺悬钩子(R.parapungens H.Hara),并提供了简要的描述和标本照片。     

Rediscoveries of two species of Rubus (Rosaceae) in China

报道了蔷薇科两种悬钩子属植物在中国的重新发现,分别为美叶悬钩子(Rubus calophyllus C.B.Clark)和拟针刺悬钩子(R.parapungens H.Hara),并提供了简要的描述和标本照片.     

西藏墨脱悬钩子属植物小志

该文在野外调查、标本采集、标本查阅与鉴定及文献考证的基础上,对西藏墨脱县产的蔷薇科悬钩子属植物进行了系统整理。结果表明:目前发现该区共有悬钩子属植物28种4变种,其中Rubus lineatus Reinw. var. glabrior Hook. f.为中国分布新记录植物,小柱悬钩子(R. columellaris Tutcher)、红毛悬钩子(R. wallichianus Wight et Arn.)、独龙悬钩子(R. taronensis C. Y. Wu ex T. T. Yu et L. T. Lu)和疏松悬钩子(R. efferatus Craib)为西藏分布新记录植物。该文还对《中国植物志》和Flora of China中该属部分学名的不恰当使用进行了探讨。     

江西悬钩子属的分类和地理分布

通过野外调查和查阅大量标本和文献,对江西悬钩子属植物的分类和地理分布进行了较为系统的研究。江西境内共有悬钩子属植物43种7变种2变型,其中红花悬钩子Rubus inopertus(Diels)Focke,大红泡R.eustephanus Focke ex Diels,深裂锈毛莓R.reflexus Ker var.lanceolobus Metc为江西新分布。编制了检索表,并讨论了江西悬钩子属植物的地理分布。     

江西悬钩子属一新种

本文发表了江西悬钩子属一新种,即腺果悬钩子Rubus glandulosocarpus M.X.Nie     

中国悬钩子属新分类群

近年来对我国9个省的悬钩子属植物资源进行了调查采集,并在南京中山植物园建立了田间种质库进行观察研究。本文报导在云南省悬钩子资源调查中发现的1个新种和5个新变种,它们是:Rubus godongensis Gu et Li, R. biflorus Buch.-Ham.ex Smith var.spinocalycinus Gu et Li, R. glabricarpus Cheng var.eglandulosus Gu et Li, R. gongshanensis Yü et Lu var.eglandulosus Gu et Li, R. parvifolius L.var.purpureus Gu et Li, R. viburnifolius Focke var.apetalus Gu etLi.     

贵州悬钩子属(蔷薇科)一新种--务川悬钩子

报道了贵州省务川县发现的悬钩子属Rubus（蔷薇科Rosaceae）一新种——务川悬钩子R．wuchuanensis S．Z．He。该种在体态上与锯叶悬钩子R．wuzhianus L．T．Lu＆Boufford相近,区别在于其叶片卵状披针形,边缘疏生小锯齿;叶柄较短,长0．7-1cm;花序为顶生稀疏总状花序,具花8-10朵：花梗长3．55cm;花瓣先端具骤突尖头。     

粗叶悬钩子叶中多酚类与三萜类化合物的研究

采用高效液相色谱-串联质谱（HPLC-MS/MS）联用技术,对中国南方民间用于治疗肝损伤和癌症的药材粗叶悬钩子（Rubus aleaefolius Poir.）叶中的多酚类和三萜类成分进行了分析。通过与文献数据和部分对照品的紫外光谱、负离子模式下的一级和二级质谱信息进行比较,从粗叶悬钩子叶中共分析了18个化合物,鉴定了其中2个多酚类化合物,推测了9个多酚类和7个三萜类化合物的可能结构,并对三萜类化合物的质谱裂解途径进行了解析。基于对三萜类化合物裂解规律的研究,推测了1个三萜,即3-对香豆酰氧基-19α-羟基熊果酸。芦丁、绿原酸和3-对香豆酰氧基-19α-羟基熊果酸在本植物中属首次报道。     

河北小五台山自然保护区寄蝇科昆虫调查

报告我国河北小五台山自然保护区分布的寄蝇科昆虫4亚科17族51属67种,其中发现4个中国新纪录属:娇寄蝇属Hebia Robineau-Desvoidy,弓寄蝇属Arcona Richter,强腹寄蝇属Strongygaster Macquart,旁寄蝇属Parhamaxia Mesnil;7个中国新纪录种:湿蓖寄蝇Billaea irrorata(Meigen)、狭额喙寄蝇Stomina angustifrons Kugler、黄娇寄蝇Hebia flavipes R.-D.、均伪膝芒寄蝇Pseudogonia parisiaca(R.-D.)、阿穆尔弓寄蝇Arcona amuricola Richter、球强腹寄蝇Strongygaster globula(Meigen)、心鬃旁寄蝇Parhamaxia discalis(Mesnil);34个河北新纪录种,13个中国特有种,并初步研究了小五台山寄蝇区系及生态习性。     

毛茛属五种植物的核型

对中国云南毛茛属（Ranunculus）5种植物核型进行研究,结果表明,毛茛组茴茴蒜（Ranunculus chinensis Bunge）和禺毛茛（R．cantoniensis DC．）核型公式为2n=2x=16=6m＋4sm＋6st和2n=4x=32：14m＋6sm＋12st;该组茴茴蒜、禺毛茛和扬子毛茛（R．sieboldii Miq．）的不同居群核型存在自西向东不对称系数渐增大现象。在美丽毛茛组中,深齿毛茛（R．pulchellusvar．stracheyanus Hand．-Mazz．）的中甸居群核型（2n=4x=32=12m＋12sin＋8st）与青海居群核型（2n：4x：32：24m＋8sm）明显不同;毛果高原毛茛（R．tangusticusvar．dasycarpus（Maxim．）L．Liou）染色体数目（2n=40）,核型公式（2n=5x=40=30m＋10sm）和纳帕海毛茛（R．napahaiensis W．T．Wang＆L．Liao）染色体数目（2n=40）,核型公式（2n=5x=40=20m＋16sin＋4st）为首次报道。     

Circumscription and phylogeny of Saxifraga sect. Ciliatae: Evidence from nrDNA ITS sequences

The largest section of the genus Saxifraga (Saxifragaceae), sect. Ciliatae, consists of 175 morphologically diverse species. This section is mainly distributed in the Qinghai-Tibetan Plateau and adjacent regions of southwest China and more than 80% of the total number of species are endemic to this region. It remains unknown whether this section is monophyletic and up to now no study has been conducted on the infra-sectional phylogeny. In this study, ITS sequences of the nuclear ribosomal DNA were firstly determined for 33 species mainly from this section and related sections. We further downloaded the corresponding sequences of the same DNA region for the other 22 species of Saxifraga and Mitella from GenBank. All sequences were together used to construct the phy-logenetic trees. The main implications of the phylogenetic analyses include: (1) sect. Ciliatae, as traditionally defined, constitutes as a monophyletic clade and its sister group is a well supported clade that includes species from 8 sections such as sect. Porphyrion, sect. Saxifraga and sect. Mesogyne; (2) three morphological subsections, i.e., subsect. Gemmiparae, subsect. Hirculoideae and subsect. Rosulares were tentatively recovered despite the relatively low statistic bootstrap support for the last one; however, subsect. Flagellares and subsect. Hemi-sphaericae were not recognized as separate entities, and nested within subsect. Gemmiparae; (3) subsect. Hircu-loideae and subsect. Rosulares clustered together as sister subclades while subsect. Gemmiparae diverged early. In addition, our results suggest that the paired variation of ITS sequences in sect. Ciliatae is relatively low between the sampled species in spite of their diverse morphology. It is suggested that such a scenario may mirror rapid speciation in this section that probably trigged by the uplifts of the Qinghai-Tibetan Plateau and the extensive selection pressure under the alpine environments.     

几种野生悬钩子家化的生物学基础研究

在对中国7个省区悬钩子属（Rubus L.）资源调查的基础上,选出蓬（R.hirsutus Thunb.）、掌叶覆盆子（R.chingii Hu）、山莓（R.corchorifolius L.）和高粱泡（R.lambertianus Ser.)4个野生种进行栽培化研究。本文报道4个种的生长结果习性、物候期、营养需要、花芽分化过程以及繁殖习性,并提出了一系列栽培措施。     

Molecular phylogeny and genetic variation in the genus Lilium native to China based on the internal transcribed spacer sequences of nuclear ribosomal DNA

We present a comprehensive phylogeny derived from nuclear ribosomal DNA (nrDNA) for 214 samples representing 98 species and five varieties, including 44 species and five varieties native to China. Our collection of 25 species and five varieties (44 samples) covering all five sections of the genus (Comber) distributed in China also were included in the internal transcribed spacer (ITS) database. This study incorporates previous research with an emphasis on Chinese species, including the controversial subsection, Sinomartagon 5c Comber. In the phylogenetic tree obtained by maximum parsimony (PAUP) and maximum likelihood (RAxML) analyses, the samples were divided into four major groups. Our results suggest that the subsection (subsect.) 5c Comber should be classified into the true subsect. 5c and the section (sect.) Lophophorum. And the latter was divided into three subsections (subsect. Lophophorum I, subsect. Lophophorum II, and subsect. Lophophorum III). Based on molecular phylogenetic analysis and fluorescence in situ hybridization, we report that L. henryi and L. rosthornii are closely related, and we propose their classification into subsect. Leucolirion 6a. Our results support Comber’s subdivision of sect. Leucolirion, which was primarily based on bulb color. Chinese species were divided into five sections: sect. Martagon, sect. Archelirion, sect. Leucolirion, sect. Sinomartagon, and sect. Lophophorum. These findings contribute to our understanding of the phylogeny, origin, and classification of Lilium.     

基于β-微管蛋白基因部分序列探讨灵芝属菌株的亲缘关系

PCR分别扩增了38个灵芝属菌株的β-微管蛋白基因片段,并对PCR产物进行序列测定,得到419bp的一段核苷酸系列.根据MEGA 2.1软件中的neighbour-joining methods对上述序列进行聚类分析,结果所有供试菌株被分成9个聚类组.中国栽培灵芝菌株分布于6个聚类组,其中树舌亚属、紫芝组的菌株各自聚成一组,灵芝组的菌株分成四组,但大部分灵芝组菌株均聚于同一组, 这表明树舌亚属、紫芝组和灵芝组间的遗传差异较大,灵芝组内虽然存在着一定的遗传差异,但总体上亲缘关系比较近,遗传多样性并不丰富.同时,序列分析的结果显示,β-tubulin基因序列在第三位密码子和内含子部位有高的碱基替换率,这些变异提供了丰富的系统发育信息,提示β-tubulin基因适合于灵芝属菌株的亲缘关系研究.     

四川铁线莲属二新种

描述了自四川东南部发现的毛茛科铁线莲属二新种:黄荆铁线莲和古蔺铁线莲。     

中国的球盖菇科(五) 广义斑褶菇属

通过观察吉林农业大学菌物研究所标本馆(HMJAU)、中国科学院微生物研究所菌物标本馆(HMAS)、广东微生物研究所标本馆(GDGM)及作者野外采集的共180份标本的宏观形态和微观结构,对中国球盖菇科的广义斑褶菇属及其所含的灰斑褶菇属(Copelandia Bres.)、疣孢斑褶菇属(Panaeolina Maire)和狭义斑褶菇属[Panaeolus(Fr.)Qul(s.str.)]进行了分类学研究,对中国分布的16个种进行了形态学描述、显微线条图绘制,并编写了分种检索表。其中,包括1个新组合:栗褶疣孢斑褶菇[Panaeolina castaneifolia(Murr.)Sarentoya et Tolgor];2个中国新记录种:热带灰斑褶菇[Copelandia tropicalis(Ola′h)SingerR.A.Weeks]、小型斑褶菇[Panaeolus alcidisM.M.Moser];12个省级新记录种:北京新记录种硬腿斑褶菇[Panaeolus solidipes(Peck)Sacc.],内蒙古新记录种硬腿斑褶菇[Panaeolus solidipes(Peck)Sacc.]、锐顶斑褶菇[Panaeolus acuminatus(Schaeff.)Qul.]、黑斑褶菇[Panaeolus ater(J.E.Lange)KhnerRomagn.]、紧缩斑褶菇[Panaeolus sphinctrinus(Fr.)Qul.]、红褐斑褶菇[Panaeolus subbalteatus(Berk.Broome)Sacc.],吉林省新记录种半卵圆斑褶菇[Panaeolus semiovatus(Sowerby)S.LundellNannf.]、栗褶疣孢斑褶菇[Panaeolina castaneifolia(Murrill)Bon],黑龙江新记录种红褐斑褶菇[Panaeolus subbalteatus(Berk.Broome)Sacc.],宁夏新记录种变蓝灰斑褶菇[Copelandiacyanescens(Berk.Broome)Singer]、锐顶斑褶菇[Panaeolus acuminatus(Schaeff.)Qul.],西藏新记录种红褐斑褶菇[Panaeolus subbalteatus(Berk.Broome)Sacc.]。