金针菇子实体多糖分离纯化及结构和免疫活性研究

从金针菇子实体中分离纯化多糖,并对多糖结构和体外免疫活性进行研究。采用水提醇沉法从金针菇子实体中提取粗多糖,利用DEAE-Cellulose-52及Sephacryl S-300HR柱层析纯化得到FVPⅠ-a,再利用HPLC-ELSD技术、红外及核磁共振对FVPⅠ-a进行结构解析。在体外以促RAW264.7巨噬细胞产NO、分泌细胞因子,促进小鼠淋巴细胞增殖实验,考察FVPⅠ-a增强免疫的能力。从金针菇子实体中分离纯化得到FVPⅠ-a,其为分子量81.4kDa,由葡萄糖、果糖和鼠李糖组成的β构型的吡喃型杂多糖。体外免疫实验表明,FVPⅠ-a能够促进RAW264.7巨噬细胞产生NO及分泌细胞因子（IL-1β,IL-6,TNF-α）,能单独的促进小鼠淋巴细胞增殖（P<0.05）,并能协同增强ConA和LPS对小鼠淋巴细胞的促增殖作用（P<0.01,P<0.05）。首次从金针菇子实体中获得FVPⅠ-a杂多糖,其在体外具有增强非特异性免疫反应及增强特异性免疫反应的能力。     

恰麻古多糖对巨噬细胞RAW264.7体外免疫调节作用初探

初步探讨恰麻古粗多糖BRP、中性多糖BRNP-1、BRNP-2及酸性多糖BRAP-1、BRAP-2对巨噬细胞RAW264.7的免疫调节作用。实验方法选用CCK-8法检测不同质量浓度各恰麻古多糖组对巨噬细胞RAW264.7细胞增殖率的影响;以中性红法观察各组恰麻古多糖对巨噬细胞RAW264.7吞噬活性的影响;Griess法测定恰麻古多糖致巨噬细胞RAW264.7对NO的释放水平;采用酶联免疫吸附法(ELISA)试剂盒检测细胞因子TNF-α(肿瘤坏死因子)与IL-6(白介素-6)分泌水平。实验结果显示不同质量浓度各恰麻古多糖组能够显著提高巨噬细胞RAW264.7增殖率与对中性红的吞噬活性,并能够刺激巨噬细胞释放NO,且促进其TNF-α及IL-6分泌水平。通过实验,初步验证了各恰麻古多糖具有良好的生物活性,并对巨噬细胞RAW264.7具有免疫调节作用。     

细胞因子在1型糖尿病中的作用

1型糖尿病是T细胞介导的以胰腺β细胞特异性损伤为特征的炎症性自身免疫疾病,侵润胰岛的巨噬细胞,淋巴细胞等产生的细胞因子如白细胞介素-1β、肿瘤坏死因子-α、干扰素-α、干扰素-γ、肿瘤坏死因子-β和白细胞介素-2等通过诱导胰腺β细胞凋亡/坏死和胰岛素分泌缺陷、调节T细胞的活化和种群比例,以及调控T细胞对β细胞的免疫识别和杀伤等,在1型糖尿病的发生和发展中起关键作用。     

鳞柄小奥德蘑多糖对巨噬细胞免疫功能的调节作用

研究鳞柄小奥德蘑多糖对小鼠巨噬细胞的免疫调节作用。采用灌洗腹腔法收集小鼠巨噬细胞,建立其体外培养体系;采用鸡血红细胞法、荧光探针标记、总一氧化氮检测和酶联免疫吸附试验等方法分别检测巨噬细胞吞噬能力、NO合成量、肿瘤坏死因子-α、白细胞介素-1、白细胞介素-6和白细胞介素-12等的分泌量。结果显示,与空白对照组相比,鳞柄小奥德蘑多糖能显著增强体外培养和腹腔内巨噬细胞的吞噬能力和NO合成量,增加体外培养的小鼠巨噬细胞对肿瘤坏死因子-α、白细胞介素-1、白细胞介素-6和白细胞介素-12等细胞因子的分泌量。因此,鳞柄小奥德蘑多糖可能通过提高细胞对NO和多种免疫相关信号分子的分泌量,增强细胞的吞噬能力,进而调节小鼠巨噬细胞的免疫功能。     

黄芪多糖联合布洛芬对创伤感染患者巨噬细胞分泌功能、T淋巴细胞亚群水平的影响及其影响因素分析

目的:探讨黄芪多糖联合布洛芬对创伤感染患者巨噬细胞分泌功能、T淋巴细胞亚群水平的影响及影响因素。方法:2014年3月-2018年3月我院收治创伤感染患者85例,其中重度感染39例,将所有患者按感染轻重进行平衡随机分为对照组43例和研究组42例,对照组应用布洛芬治疗,研究组应用黄芪多糖联合布洛芬治疗。比较两组患者巨噬细胞分泌功能、T淋巴细胞亚群水平,分析患者发生重度感染的影响因素及巨噬细胞分泌功能、T淋巴细胞亚群水平与创伤感染的关系。结果:治疗后研究组患者血清中肿瘤坏死因子(TNF)、白介素细胞-6(IL-6)和白介素细胞-1(IL-1)的水平低于对照组(P<0.05)。治疗后研究组外周血中CD3⁺、CD4⁺、CD8⁺和CD4⁺/CD8⁺水平高于对照组(P<0.05)。巨噬细胞分泌功能较高、T淋巴细胞亚群水平较低的患者发生重度感染的概率较高(P<0.05);巨噬细胞分泌功能与创伤感染呈正向关联关系(P<0.05,OR>1);T淋巴细胞亚群水平与创伤感染呈负向关联关系(P<0.05,OR<1);治疗方法以研究组方法为优(P<0.05,OR>1)。结论:黄芪多糖联合布洛芬治疗创伤感染患者效果更显著,巨噬细胞分泌功能、T淋巴细胞亚群水平均是创伤感染的影响因素。     

杨梅素对环磷酰胺诱导的免疫抑制小鼠的免疫功能影响CSCD

黄酮类化合物杨梅素(myricetin,MYR)具有抗肿瘤、抗氧化等多种药理作用,但在免疫调节方面的作用尚未完全了解,因此本实验通过建立环磷酰胺(cyclophosphamide,CTX)免疫低下小鼠模型来研究MYR对免疫功能的影响。通过称重小鼠脾脏和胸腺以及计算指数评价MYR对模型小鼠症状的缓解程度,用MTT法、溶血试验、酶联免疫吸附法等免疫学相关实验技术检测T、B淋巴细胞增殖,T细胞亚群分布情况,血清溶血素含量,抗体形成细胞数以及细胞因子白介素-2、白介素-4、白介素-6、干扰素-γ的分泌水平。实验数据表明,MYR能提高小鼠免疫器官脾脏、胸腺指数,促进T淋巴细胞和B淋巴细胞的分泌,提高细胞因子水平和抗体形成细胞数量。结果表明MYR能在免疫反应中起积极作用,调节免疫细胞和细胞因子之间的相互作用恢复机体免疫力的平衡,改善免疫力低下的症状。     

马齿苋多糖对S180荷瘤小鼠免疫功能的影响

本文探讨马齿苋多糖对S180荷瘤小鼠免疫功能的影响。马齿苋采用水提醇沉法得到马齿苋多糖,分别以50、100、200mg／kg通过腹腔给药10d,观察马齿苋多糖对S180荷瘤小鼠的抑瘤作用及对小鼠淋巴细胞转化功能、腹腔巨噬细胞的吞噬能力、白介素-l（IL-1）和白介素-2（IL-2）生成量的影响。结果显示,马齿苋多糖对S180荷瘤小鼠有明显的抑瘤作用,抑瘤率分别为16．92％、51．45％和64．96％。不同剂量马齿苋多糖与对照组相比可明显促进淋巴细胞的转化、小鼠腹腔巨噬细胞吞噬能力,可有效的增加荷瘤小鼠脾淋巴细胞的转化和腹腔巨噬细胞的吞噬能力以及白介素-1（IL-1）和白介素-2（IL-2）的分泌。说明马齿苋多糖对S180荷瘤小鼠具有显著的抗肿瘤作用,其作用机制与增强小鼠免疫作用有关。     

香菇多糖的生物活性

香菇多糖 (ｌｅｎｔｉｎａｎ ,ＬＮＴ)是从伞菌科真菌香菇(ｌｅｎｔｉｎｕｓｅｄｏｄｅｓ)的子实体中分离到的一种 β 1,3 葡聚糖 ,2 0世纪 6 0年代日本科学家首先证明其具有显著的免疫调节活性和抗肿瘤活性 ,经临床验证 ,已在国际市场上推广应用。1.香菇多糖免疫调节活性香菇多糖的免疫调节活性是其生物活性的重要基础。香菇多糖是典型的Ｔ细胞激活剂 ,体内外均能促进细胞毒Ｔ淋巴细胞 (ＣＴＬ)的产生 ,提高ＣＴＬ的杀伤活力 ,增强正常或免疫功能低下小鼠的迟发型超敏反应 (ＤＴＨ) ,提高抗体依赖性细胞毒细胞(ＡＤＤＣ)活性。香菇多糖在体…     

茶树花多糖免疫调节与抗肿瘤活性的研究

茶树花富含多糖,是茶树生长过程中的产物,然而目前茶树花多糖(tea plant flow polysaccharide, TFP)尚未被充分利用．因此,认为茶树花多糖有着和茶叶中的多糖相似的功能效果．研究目的是通过系列的体内和体外实验系统来评价茶树花多糖的抗肿瘤以及免疫调节的活性．利用S180(sarcoma 180)荷瘤小鼠模型,系统地研究了灌胃不同剂量茶树花多糖对荷瘤小鼠的肉瘤抑制率、存活率以及细胞免疫的影响．结果显示,连续灌胃10天茶树花多糖,能显著抑制S180肉瘤的生长,延长荷瘤小鼠的存活时间,增强迟发型超敏作用(delayed-type hypersensitivity, DTH),促进血液白介素-2(IL-2)、γ-干扰素(IFN-γ)的分泌,增强巨噬细胞的吞噬作用,改善T淋巴细胞亚群CD4⁺数量以及CD4⁺/CD8⁺的比值,表明茶树花多糖能增强机体对肿瘤的防御,在一定程度上归功于其对免疫调节作用．     

4－硒（代）硫酸酯多糖的抗肿瘤免疫调节作用及其机制研究

本实验系统地研究了４－硒（代）硫酸酯多糖的抗肿瘤免疫调节作用及其机制。发现该药在体内外均有抑制肿瘤生长的作用,并认为其抑瘤机制与促进巨噬细胞活性,间接促进淋巴细胞活性,释放具有杀伤肿瘤细胞的效应分子以及选择性抑制肿瘤细胞大分子合成有关。结果表明,４－硒（代）硫酸酯多糖是一种兼具杀伤肿瘤细胞和增强免疫功能双重作用的新型免疫型抗肿瘤药。     

牛樟芝子实体醇提物对过敏性肺炎的作用

本文研究台湾特有牛樟芝子实体醇提物对过敏性肺炎的作用并探讨其可能的作用机制。体内实验采用卵清蛋白（OVA）致敏制备过敏性肺炎模型,观察各组肺组织病理变化情况,并对脂多糖（LPS）诱导的细胞分泌促炎性细胞因子进行检测;离体实验中采用酶联免疫吸附法（ELISA）对LPS刺激THP-1细胞分泌促炎性因子（IL-1、IL-6及TNF-α）进行了检测。体外巨噬细胞（THP-1）抗炎试验结果显示牛樟芝子实体醇提物不同浓度均可显著抑制LPS诱导的THP-1细胞IL-1的分泌（P<0.001）;可完全抑制IL-6的分泌;浓度20μg/mL及80μg/mL下可以完全抑制TNF-α的分泌。OVA过敏性肺炎动物模型中,与模型组相比,牛樟芝子实体醇提物组可显著改善肺部外观色泽、细支气管淋巴球聚集、肺泡壁增生及肺泡空腔等炎症程度。口服KBA皿式牛樟芝子实体醇提物可有效改善过敏性肺炎程度,并有全身系统性抗炎效果,其机制可能为抑制THP-1细胞IL-1、IL-6及TNF-α的分泌。本研究为深入研究KBA皿式牛樟芝子实体改善雾霾性肺炎提供了重要的实验依据。     

The role of cytokines in the regulation of Leydig cell P450c17 gene expression

Cytokines produced by immune-activated testicular interstitial macrophages (TIMs) may play a fundamental role in the local control mechanisms of testosterone biosynthesis in Leydig cells. We investigated whether in vivo immune-activation of TIMs can modulate Leydig cell steroidogenesis. To immune activate TIMs in vivo, mice were injected intraperitoneally (i.p.) with lipopolysaccharide (LPS, 6 mg/kg). TIMs and Leydig cells were purified for RNA analysis. LPS treatment resulted in a 47-fold increase in interleukin-1β (IL-1β) mRNA in TIMs. P450c17 mRNA levels in the Leydig cells from the same animals, decreased to less than 10% compared to control. The effect of LPS on IL-1β and P450c17 mRNA levels was reversible on both TIMs and Leydig cells, respectively. To determine if the effect of LPS on P450c17 was mediated by a possible decrease in pituitary LH secretion, mice were co-injected with LPS and hCG. Treatment with hCG did not change the effect observed with LPS alone, in TIMs or in Leydig cells. In vitro, LPS treatment of TIMs resulted in marked induction of IL-1β mRNA expression. In parallel, in vitro treatment of Leydig cells with recombinant IL-1 resulted in a dose-dependent inhibition of P450c17 mRNA expression and testosterone production. These data demonstrate that LPS treatment, in vivo and in vitro, induced IL-1 gene expression in TIMs, and that IL-1 inhibits P450c17 mRNA in vitro. Therefore, we suggest that immune-activation of TIMs might have caused the observed inhibition of P450c17 gene expression in Leydig cells in vivo.     

黄芪多糖对咪喹莫特诱导小鼠银屑病样皮炎的干预作用及其机制*

目的: 探讨黄芪多糖对咪喹莫特诱导的小鼠银屑病样皮炎的干预作用及其机制。方法: 40只健康雌性C57BL/6小鼠随机分为空白对照组、模型组、黄芪多糖高剂量组(200 mg/kg)、中剂量组(100 mg/kg)和低剂量组(50 mg/kg),共5组,每组8只,利用5%咪喹莫特乳膏涂抹小鼠背部制备银屑病样皮炎模型,监测各组小鼠PASI评分,并采用ELISA测定小鼠炎症因子分泌的变化,通过流式细胞术检测皮炎中巨噬细胞的浸润程度。结果: 与空白对照组比较,模型组小鼠PASI评分明显增加(P＜0.05),血清TNF-α、IL-1β和IL-6水平明显升高(P＜0.05),皮肤组织中浸润的巨噬细胞明显增加(P＜0.05);与模型组比较,黄芪多糖高、中剂量组小鼠PASI评分明显明显减少(P＜0.05),小鼠血清TNF-α、IL-1β和IL-6水平明显下调(P＜0.05);黄芪多糖高剂量组小鼠皮肤组织中浸润的巨噬细胞明显减少(P＜0.05)。结论: 黄芪多糖通过抑制皮肤组织中巨噬细胞浸润,降低血清中TNF-α、IL-1β和IL-6的分泌,来改善小鼠银屑病样皮炎。     

Immunomodulatory activity of polysaccharide isolated from Angelica sinensis

The immunomodulatory activities of an Angelica sinensis polysaccharide (AP), purified from the fresh root of A. sinensis Diels, were investigated in vitro in relation to the specificity to immune cells. AP consisted of rhamnose, arabinose, mannose, glucose, galactose with the molar ratio of 1.00:4.54:2.98:11.09:7.45. Cell proliferation results showed that proliferation of total spleen cells, macrophages and T cells were promoted by the action of AP. The treatment of AP increased the production of IL-2 and IFN-γ, while that of IL-4 was decreased. RT-PCR analysis displayed that the IL-2 and IFN-γ gene expression were enhanced but the IL-4 gene expression was decreased. Some differences in cytokines secretion pattern were also detected, the expression of IFN-γ was rapidly augmented while that of IL-2 responded later. The flow cytometry results showed that the percentage of CD4⁺T cell in total spleen cells was remarkably increased by AP, while that of CD8⁺T cell was slightly decreased. In conclusion, AP has immunomodulatory activity by regulating expression of Th1 and Th2 related cytokines. The time–effect relation of cytokines response also suggests that macrophages and natural killer cells involved in nonspecific immunity were primary activated, and helper T cell were secondarily affected by AP.     

Pre-Weaning Growth Hormone Treatment Ameliorates Bone Marrow Macrophage Inflammation in Adult Male Rat Offspring following Maternal Undernutrition

Maternal undernutrition (UN) is associated with the development of obesity and metabolic complications in adult offspring. While the role of inflammation in obesity and related comorbidities has been well established, there is little evidence regarding the effects of maternal UN-induced programming on immune function in male adult offspring. This study examines the effects growth hormone (GH), which is known to induce anti-inflammatory effects, on maternal UN-induced bone marrow macrophage (BMM) function in adult male offspring. Sprague-Dawley rats were assigned to chow (C) or UN (50% ad libitum; UN) diet throughout gestation. Male C and UN pups received saline (CS/UNS) or GH (2.5 µg/g/d; CGH/UNGH) from day 3–21. Bone marrow hematopoietic cells were differentiated to a macrophage phenotype in the presence of M-CSF (50 ng/ml). Differentiated bone marrow macrophages (BMM) were stimulated with LPS (100 ng/ml) for 6 h. UNS-derived BMM had significantly increased secretion and expression of IL-1β and IL-6 following LPS stimulation. This was accompanied by increased expression of IL-1R1, IL-6R and TLR4. Pre-weaning GH treatment reversed this pro-inflammatory phenotype. Furthermore UNGH displayed increased expression of markers of alternative (M2) macrophage activation, mannose receptor and PPARγ. This study demonstrates that fetal UN exposure primes hematopoietic immune cells to a more potent pro-inflammatory phenotype with heightened cytokine secretion and receptor expression. Furthermore these cells are pre-disposed to pro-inflammatory M1 macrophage phenotype which has wide-reaching and important effects in terms of obesity and metabolic disease.     

猴头菌不同发育阶段产生的多糖结构特征及免疫活性

采收猴头菌Hericium erinaceus 7个不同发育阶段的子实体,经热水浸提后分别采用20%、50%、70%的乙醇终浓度进行分级沉淀,获得21个多糖组分,对它们的结构特征及体外免疫活性进行了研究。结果表明,在猴头菌发育过程中,多糖总得率呈现先增大后减小的趋势,在第5阶段达到最大值0.92%。20%醇沉的多糖含量也呈先增大后减小的趋势,在第5阶段达到最大值42.87%,其大分子量多糖（1 000-5 000kDa）所占比例最高。50%、70%醇沉多糖均为小分子量多糖,约为10-40kDa。获得的猴头菌多糖组分中,单糖组成多以岩藻糖、半乳糖、葡萄糖和甘露糖为主（相对比例存在一定差异）;另外,第1阶段20%、70%醇沉多糖还含有少量的核糖,第7阶段20%醇沉多糖含有一定量的鼠李糖;7个时期50%醇沉多糖均含有一定量的葡萄糖醛酸。所得多糖样品均具有刺激巨噬细胞释放NO的活性,其中20%醇沉多糖的活性优于50% 和70% 醇沉多糖,在50μg/mL时就表现出显著的体外免疫活性。此外,在第5阶段即中菌刺期产生的多糖活性最优,说明在此阶段采摘可以获得最佳的猴头菌多糖原材料。本研究为猴头菌生长发育过程中活性多糖的动态形成研究提供了一定的理论基础。     

Murine T cell clones specific for Hymenolepis nana: generation and functional analysis in vivo and in vitro.

To examine the role of the T cell in protective immunity to Hymenolepis nana, H. nana-specific clonal lymphocytes were generated from mesenteric lymph nodes of BALB/c mice infected with H. nana, and some of their functions were analyzed in vitro and in vivo. Following limiting dilution techniques, five clones were generated from mesenteric lymph node cell populations. All of these clones expressed the L3T4⁺, Lyt-2.2⁻ phenotype and proliferated in vitro in response to soluble egg antigen of H. nana. Of five clones, three secreted interleukin 2 (IL-2) and interferon-γ (IFN-γ) after stimulation with egg antigen. Furthermore, these three clones conferred local delayed-type hypersensitivity to egg antigen. The remaining two clones produced interleukin 4 (IL-4) in response to egg antigen, and could not mediate local delayed-type hypersensitivity. Adoptive transfer experiments using clonal lymphocytes were also undertaken in an attempt to define cell types involved in protective immunity. Clonal lymphocytes secreting both IL-2 and IFN-γ transferred protective immunity, equivalent to that obtained by non-cultured-sensitized mesenteric lymph node cells. They were effective in very small numbers. However, clonal lymphocytes that secreted IL-4 did not transfer protective immunity. These results suggest that helper T lymphocytes, especially the Th1 subtype, are involved in protective immunity against H. nana.     

Interleukin 2 inhibits in vitro granulocyte-macrophage colony formation

We have previously shown that murine bone marrow cells cultured with interleukin 2 (IL-2) produce interferon-alpha/beta (MuIFN-alpha/beta) and that IFN-alpha/beta can suppress in vitro granulocyte-macrophage colony-forming cell formation (GM-CFC). In this study, IL-2 was directly assessed for its ability to inhibit in vitro granulocyte and/or macrophage colony-forming cell formation (GM-CFC/M-CFC). C57BL/6 bone marrow cells were cultured with different colony-stimulating factors (CSF), i.e., partially purified macrophage-CSF (M-CSF) or recombinant granulocyte and macrophage CSF (GM-CSF) in the presence or absence of different IL-2 preparations. Partially purified mouse IL-2 or recombinant human or mouse IL-2 (rHuIL-2 and rMuIL-2) totally inhibit GM-CFC and M-CFC formation at 7 days of culture. The level of inhibition mediated by IL-2 was concentration-dependent, with as little as 1 U/ml giving total inhibition of colony formation. The ability of IL-2 to inhibit colony formation was completely abolished by treatment with antisera to IL-2. MuIFN-alpha/beta and MuIFN-gamma appeared to play no role in IL-2-induced myelo-suppression in that addition of antisera to these IFN failed to block IL-2-induced suppression. Myelo-suppression mediated by IL-2 was independent of the concentration of CSF used in the bone marrow cultures. Suppression was also not dependent upon the initial presence of T cells or natural killer (NK) cells. Bone marrow cells depleted of Thy-1+, Lyt-1+, Lyt-2+, NK-1.1+, Asialo GM1+, or Qa-5+ cells were as susceptible to IL-2 induced suppression as untreated or complement-treated bone marrow cells. These results suggest that IL-2 may play an important role in regulating different aspects of hematopoiesis.     

T-cell depletion of allogeneic bone marrow prevents acceleration of graft-versus-host disease induced by exogenous interleukin 2

Highly purified human recombinant interleukin 2 (IL-2) markedly accelerated lethal GVHD in the H-2-identical B10.BR----CBA combination, but had no effect when the donor cells were depleted of mature (Thy-1.2-positive) T lymphocytes, indicating a strong immunopotentiating effect of IL-2 on mature T cells causing GVHD. In the same donor-host combination, IL-2 did not influence the recovery from the post-transplantation bone marrow aplasia. The results suggest that IL-2 could be considered for adjuvant hormonal therapy to enhance immune recovery in recipients of T-cell-depleted allogeneic marrow.     

广叶绣球菌β-D-葡聚糖对巨噬细胞RAW264.7免疫调节作用受体TLR4和TLR2的影响

确定广叶绣球菌β-D-葡聚糖对巨噬细胞RAW264.7的免疫调节作用受体,探索广叶绣球菌β-D-葡聚糖的免疫调节机制。采用MTT法测定不同浓度广叶绣球菌β-D-葡聚糖对巨噬细胞RAW264.7增殖活力的影响,筛选出促进巨噬细胞增殖能力最强的浓度。用筛选出的β-D-葡聚糖浓度作用巨噬细胞RAW264.7;TLR4抗体和TLR2抗体分别作用巨噬细胞RAW264.7 1h,再用含有β-D-葡聚糖的细胞培养液培养。收集细胞培养上清和细胞,检测细胞培养上清中NO、IL-6、TNF-α、IFN-β的生成量;提取细胞内总RNA,采用RT-PCR测定巨噬细胞TLR4 mRNA表达量;提取巨噬细胞总蛋白,采用蛋白免疫印迹western blot测定TLR4的蛋白表达。广叶绣球菌β-D-葡聚糖能够促进巨噬细胞RAW264.7增殖,增加NO、IL-6、TNF-α、IFN-β的生成量,提高TLR4 mRNA表达和蛋白表达,差异极显著（P<0.01）。TLR4抗体作用细胞后,NO、IL-6、TNF-α、IFN-β的生成量明显下降,差异极显著（P<0.01）。TLR2抗体作用细胞后,NO、IL-6、TNF-α、IFN-β的生成量下降,但差异不显著。广叶绣球菌β-D-葡聚糖可以通过细胞表面受体TLR4激活信号转导通路,增强下游细胞因子的释放,从而调节巨噬细胞RAW264.7的免疫功能。TLR2可能不是广叶绣球菌β-D-葡聚糖的免疫受体。