11-羰基-β-乙酰乳香酸抗氧化胃保护作用及机制研究

目的:研究11-羰基-β-乙酰乳香酸(AKBA)的抗氧化作用及胃保护效果。方法:30只SD大鼠随机分为5组:正常组(生理盐水5 mL/kg),模型组(吲哚美辛48 mg/kg),西咪替丁组(100 mg/kg),低剂量AKBA组(100 mg/kg),高剂量AKBA组(200mg/kg)。预给药1小时后,用吲哚美辛(48 mg/kg)灌胃造模,评价溃疡指数(UI)、胃内容物酸度(p H)、胃壁黏液量(GWM)、前列腺素E2(PGE-2)及一氧化氮(NO)的含量,并检测超氧化物歧化酶(SOD)、过氧化氢酶(CAT)的活性,丙二醛(MDA)含量及核转录相关因子2(Nrf2)和血红素加氧酶1(HO-1)的表达量。结果:(1)与造模组相比,AKBA高剂量组(P0.05)与西咪替丁组(P0.001)显著降低了吲哚美辛诱导的胃粘膜损伤,AKBA低剂量组的差异无统计学意义;(2)AKBA与西咪替丁均显著升高胃内容物p H(P0.001)(AKBA低剂量组P0.05),增加GWM含量(P0.001);(3)与造模组相比,AKBA及西咪替丁组均显著增高PGE-2及NO含量(P0.001);(4)与造模组相比,AKBA及西咪替丁组均显著增高SOD活性(P0.001)(AKBA低剂量组P0.05)及CAT活性(P0.001)并降低了MDA含量(P0.001);(5)与造模组相比,AKBA高剂量组及西咪替丁组促进Nrf2及HO-1表达明显增高(P0.05)。结论:AKBA有较好的胃保护效果,同时可以通过升高GWM、降低胃内容物p H,升高NO含量,阻止PGE-2的降低,同时恢复SOD、CAT的活性,降低MDA含量,促进Nrf2及HO-1的表达发挥胃黏膜保护的作用。     

麦角甾甙对实验动物消化功能的影响

麦角甾甙（10mg/kg、50mg/kg）给动物连续灌胃数天可加强正常小鼠胃排空和肠推进性蠕动,拮抗新斯的明引起的胃肠运动亢进和吗啡引起的胃肠运动抑制;麦角甾甙能提高正常大鼠胃蛋白酶活性,但对胃液分泌量和胃总酸度无明显影响;麦角甾甙可加强在体兔回盲部肠管和直肠的收缩力。     

辣椒素预防大鼠胃缺血再灌注损伤的作用及机制研究

本研究为探讨辣椒素(CAP)对大鼠胃缺血再灌注损伤(GI-R)的作用及机制。成年雄性健康SD大鼠40只,随机分为4组,每组10只,A组(空白对照组),B组(手术组),C组(CAP组),D组(CAP+手术组)。给予A组、B组大鼠灌胃生理盐水10 m L/kg/d,C组、D组大鼠灌胃CAP1 mg/kg/d。4周后B组、D组于GI-R模型建立后24小时处死大鼠,收集胃液测定胃酸总酸度,肉眼计数胃黏膜损伤指数,行胃窦组织病理学观察,检测胃黏膜辣椒素受体(TRPV1)、降钙素基因相关肽(CGRP)的表达,检测胃窦组织丙二醛(MDA)、超氧化物歧化酶(SOD)的水平。结果显示A组、C组胃黏膜未见损伤;与B组相比,D组胃黏膜损伤程度显著减轻(P0.05),胃酸总酸度显著降低(P0.05),MDA值显著降低(P0.05),SOD值显著升高(P0.05),TRPV1、CGRP表达显著增多(P0.05)。实验结果表明预先给予CAP 1 mg/kg/d连续灌胃4周具有预防缺血再灌注致大鼠胃黏膜损伤的作用,其机制可能与CAP上调TRPV1、CGRP在胃内的表达、减轻氧自由基损伤有关。     

杜香不同提取部位的镇痛抗炎作用研究

采用小鼠醋酸扭体法和角叉菜胶致小鼠足掌肿胀模型筛选杜香三种提取部位镇痛抗炎作用.结果显示,甲醇提取物(10.0、1.0 mg/kg)和水提物(10.0 mg/kg)能显著抑制醋酸引起的小鼠扭体反应和角叉菜胶引起的小鼠足趾水肿.水提物(10.0 mg/kg)在致炎后2～4 h内效果接近吲哚美辛.高效液相色谱结果提示甲醇提取物的镇痛抗炎效果可能通过其所含黄酮类化合物实现.     

铁皮石斛抗阿司匹林诱导急性胃黏膜损伤活性组分筛选及作用研究

筛选铁皮石斛抗阿司匹林诱导急性胃黏膜损伤的活性组分,并探讨其抗胃粘膜损伤的作用。体外采用阿司匹林诱导人胃黏膜上皮GES-1细胞氧化应激损伤,铁皮石斛各组分预保护GES-1细胞24 h,MTS法检测细胞存活率,并通过比色法测定细胞上清液中乳酸脱氢酶LDH含量;体内采用阿司匹林建立SD大鼠急性胃黏膜损伤模型,灌胃给予铁皮石斛各组分,连续给药7 d后以溃疡指数评价铁皮石斛各组分抗阿司匹林致胃黏膜损伤作用,并通过测定胃液量、总酸度、胃蛋白酶活力、血清中一氧化氮(NO)、肿瘤坏死因子-α(TNF-α)、白介素-6(IL-6)以及胃组织中超氧化物歧化酶(SOD)、丙二醛(MDA)、前列腺素E2(PGE2),分析其可能的作用机制。结果表明,与模型组比较,铁皮石斛多糖组受损细胞活力显著升高,细胞上清液LDH含量显著降低,其它各组分细胞存活率及细胞上清液LDH含量与模型组无显著性差异;与模型组比较,铁皮石斛多糖组分显著降低胃溃疡模型大鼠的胃溃疡指数,抑制胃液分泌和胃酸含量及降低胃蛋白酶活性;与模型组比较,铁皮石斛多糖各组大鼠血清NO明显升高,TNF-α、IL-6显著降低,铁皮石斛多糖各组大鼠胃组织MDA水平明显降低,SOD、PGE2水平显著升高。铁皮石斛多糖有显著的抗阿司匹林诱导胃黏膜损伤作用。     

α-乳香酸联用羟基红花黄色素A抗胃溃疡效果研究

目的:研究乳香红花提取物中主要药效物质α-乳香酸(α-BA)和羟基红花黄色素A(HSYA)联合应用的胃保护效果。方法:36只SD大鼠随机分为6组:正常组(生理盐水5 m L/kg),模型组(生理盐水5 m L/kg),西咪替丁组(100 mg/kg),HSYA组(100mg/kg),α-BA组(200 mg/kg),联合用药组(HSYA 50 mg/kg+α-BA 100 mg/kg)。预给药1小时后,用乙醇诱导SD大鼠胃溃疡造模,评价溃疡指数、胃内容物酸度、胃壁黏液量,用ELISA法检测血清炎性因子TNF-α、IL-1β、IL-8。结果:1与造模组相比,α-BA组和联合用药组显著降低乙醇诱导胃粘膜损伤(P0.001)且联合用药组较西咪替丁组相比差异无统计学意义,但HSYA组差异无统计学意义;与造模组相比,α-BA组和联合用药组显著升高胃内容物p H,增加胃黏液含量(P0.001)且联合用药组与西咪替丁组相比差异无统计学意义,但HSYA组仅胃黏液含量有一定升高(P0.05),胃内容物p H没有明显改变。2与造模组相比,HSYA组、α-BA组、联合用药组均显著抑制血清TNF-α、IL-1β、IL-8的升高(P0.001)且联合用药组与西咪替丁组相比差异无统计学意义。3HE染色观察可见HSYA组依然有明显出血性损伤;西咪替丁组、α-BA组和联合用药组则明显保护胃黏膜、抑制损伤和白细胞浸润。结论:α-BA有较好的胃保护效果,HSYA胃保护效果显著低于α-BA,但联合应用HSYA、α-BA能够抑制胃黏膜出血、白细胞浸润、抑制胃内容物酸度,增加胃黏液含量,并且能够调节血清炎性因子从而发挥较单用药用量更少且效果更好的胃保护作用。     

马齿苋抗大鼠乙醇型胃溃疡作用研究

探讨马齿苋抗大鼠急性乙醇型胃溃疡作用。SD大鼠随机分为正常、模型、雷尼替丁及马齿苋高、中、低剂量组。给药后第14 d测定胃黏膜的溃疡指数、血清和胃黏膜组织中丙二醛(MDA)、一氧化氮(NO)、表皮生长因子(EGF)的含量和超氧化物歧化酶(SOD)活性、胃液总酸度及胃蛋白酶活性。结果表明中、高剂量马齿苋显著降低胃溃疡指数、大鼠血清中MDA含量,显著增加血清中NO、胃黏膜中EGF的含量及胃黏膜中SOD活性。高剂量马齿苋显著增加血清SOD活性,减少胃黏膜MDA含量、胃液总酸度及减小胃蛋白酶活性。马齿苋有显著的抗大鼠急性乙醇型胃溃疡作用。     

alpha- 乳香酸联用羟基红花黄色素A抗胃溃疡效果研究

目的：研究乳香红花提取物中主要药效物质alpha-乳香酸（alpha-BA）和羟基红花黄色素A（HSYA）联合应用的胃保护效果。方法： 36 只SD 大鼠随机分为6 组：正常组（生理盐水5 mL/kg）,模型组（生理盐水5 mL/kg）,西咪替丁组（100 mg/kg）,HSYA 组（100 mg/kg）,alpha-BA 组（200 mg/kg）,联合用药组（HSYA 50 mg/kg+alpha-BA 100 mg/kg）。预给药1 小时后,用乙醇诱导SD 大鼠胃溃疡造 模,评价溃疡指数、胃内容物酸度、胃壁黏液量,用ELISA法检测血清炎性因子TNF-alpha、IL-1beta、IL-8。结果：①与造模组相比,alpha-BA 组和联合用药组显著降低乙醇诱导胃粘膜损伤（P＜0.001）且联合用药组较西咪替丁组相比差异无统计学意义,但HSYA组差异 无统计学意义;与造模组相比,alpha-BA组和联合用药组显著升高胃内容物pH,增加胃黏液含量（P＜0.001）且联合用药组与西咪替 丁组相比差异无统计学意义,但HSYA 组仅胃黏液含量有一定升高（P＜0.05）,胃内容物pH 没有明显改变。②与造模组相比, HSYA 组、alpha-BA组、联合用药组均显著抑制血清TNF-alpha、IL-1beta、IL-8 的升高（P＜0.001）且联合用药组与西咪替丁组相比差异无统 计学意义。③H&E 染色观察可见HSYA组依然有明显出血性损伤;西咪替丁组、琢-BA组和联合用药组则明显保护胃黏膜、抑制 损伤和白细胞浸润。结论：alpha-BA有较好的胃保护效果,HSYA胃保护效果显著低于alpha-BA,但联合应用HSYA、alpha-BA能够抑制胃 黏膜出血、白细胞浸润、抑制胃内容物酸度,增加胃黏液含量,并且能够调节血清炎性因子从而发挥较单用药用量更少且效果更 好的胃保护作用。     

尖顶羊肚菌对急性酒精性胃黏膜损伤保护作用研究

研究尖顶羊肚菌菌丝体水提液对酒精引起的大鼠急性胃黏膜损伤的保护作用。以95%乙醇诱导的大鼠胃黏膜损伤为模型,测定各组胃黏膜损伤指数,并测定胃黏膜超氧化物歧化酶（SOD）、丙二醛（MDA）、谷胱甘肽（GSH）含量,采用幽门结扎法,测定大鼠胃酸、胃蛋白酶与胃黏液分泌的量。结果表明羊肚菌中、高剂量能明显降低胃黏膜损伤指数（p＜0.05）;羊肚菌不能抑制胃酸的分泌（p＞0.05）,但是能增加胃蛋白酶与胃黏液的分泌（p＜0.05）;95%乙醇能引起胃黏膜SOD活性与GSH的降低,MDA含量的增加,给予羊肚菌能明显抑制这一现象。结果说明羊肚菌对急性酒精性胃黏膜损伤的保护作用是与增加胃黏液分泌与提高机体抗氧化能力有关。     

尼古丁影响胃粘膜血流量和胃酸分泌

在临床上经常观察到,吸烟者胃及十二指肠溃疡的发病率要比不吸烟者高得多,同时,其消化性溃疡的愈合率也低。这是否与上消化道粘膜血流量减少、粘膜的完整性受到破坏的因素有关?最近,德国Sonnenberg等分别通过静脉内滴注尼古丁(nicotine)和直接吸烟,观察对胃粘膜血流量和胃酸分泌的影响。实验在八名不吸烟的和五名吸烟的、无上消化道病史的成年健康男性身上进行。实验前一天晚上禁食。整个实验时间为四小时,在前两小时,由静脉滴注五肽胃泌素;在后两个小时,(1)将不吸烟者分成四组,由静脉同时滴注五肽胃泌素和不同剂量(2.5、5、7.5、10μg/kg/hr)尼古丁;(2)给五名吸烟者各吸入香烟5支(每支含尼古丁0.4mg)。在实验开始时,受试者静脉内给予一定量中性红,然后按规定时间抽血,测定胃中性红清除率,作为反映胃粘膜血流量的指标,同时,通过胃管抽取胃液测定胃液量、胃酸排出量以及R值(即胃中性红清除率和胃分泌量的比率)。在实验的第二小时,上述各项指标均达到稳定状态,作为正常值。此时,胃液量为80ml/15min、胃酸分泌量为7.5mMol/15min,中性红清除率为74ml/min左右,R值为14。当静脉滴注不同剂量尼古丁后,胃液量、胃酸排出量、中性红清除率均降低,尤以前两者所受的抑制作用更为明显,R值升高,而且     

宣木瓜中多糖含量测定

目的:建立宣木瓜中多糖含量的测定方法,并测定不同采收时期的宣木瓜多糖含量。方法:在同一种植基地定期采集同一宣木瓜品种果实,以葡萄糖为对照品,采用分光光度法,以苯酚—浓硫酸为显色剂,在490 nm波长处测定吸光度,计算样品中多糖含量。结果:宣木瓜果实在生长过程中,其多糖含量在2.51%~5.08%之间,并于7月8日含量最高为5.08%。结论:本文建立的宣木瓜多糖含量的测定方法简便灵敏,精密度高,重现性好,稳定可靠;若要利用多糖含量高的宣木瓜,应适宜于7月上旬或中旬采收。     

Gastroprotective effect of leptin in indomethacin-induced gastric injury

This study investigated the involvement of neutrophil infiltration, disturbances in nitric oxide (NO) generation and oxidative stress in indomethacin-induced gastric ulcer, and the possible gastroprotective potentials of leptin, known for its angiogenic effect. Male Wistar albino rats (180–220 g) were allocated into a normal control group, ulcer control group (received a single dose of indomethacin 40 mg/kg p.o.) and an ulcer group pretreated with leptin (10 μg/kg i.p. 30 min before ulcer induction). The animals were killed 6 h after indomethacin administration and their gastric juice, serum and mucosal tissue were used for gastric injury evaluation. Indomethacin produced multiple lesions in glandular mucosa, evidenced by marked increase in gastric ulcer index (GUI) accompanied by significant increases in gastric juice acidity, tissue myeloperoxidase (MPO) activity, serum NO and tissue conjugated diene (CD), and marked decreases in tissue NO and glutathione (GSH) as well as glutathione reductase (GR) and superoxide dismutase (SOD) activities, while gastric juice mucin and tissue glutathione peroxidase (GPx) were not affected. Leptin exerted significant gastroprotection as evidenced by significantly decreased GUI and attenuated neutrophil infiltration. Leptin significantly increased mucin and tissue NO, restored GR and SOD activities and up-regulated GPx activity. It failed to affect acidity, serum NO, GSH and CD. These results suggest that leptin confers significant gastroprotection against indomethacin-induced injury through interfering with neutrophil infiltration, NO production and oxidative stress.     

Protective and therapeutic effects of Argyreia speciosa against ethanol-induced gastric ulcer in rats

The protective and therapeutic effects of Argyreia speciosa Sweet (Convolvulaceae) against ethanol-induced gastric ulcer in rats were evaluated. Ethanolic and water extracts of the aerial plant parts (200 mg/kg body weight) were orally administered daily for seven days prior to or after ulceration with one oral dose of 1 mL absolute ethanol on 24-h empty stomachs. Rats were divided into eleven groups. Group 1 served as control. To groups 2 and 3 each extract was administered. Groups 4 to 6 received each extract or ranitidine (100 mg/kg body weight) prior to ulcer induction. Groups 7 to 9 received each extract or ranitidine post ulcer induction. Groups 10 and 11 were gastric ulcerative rats after one hour and one week of ethanol induction. The evaluation was done through measuring ulcer indices: stomach acidity and volume, lesion counts, mucus, and prostaglandin E2 contents. Oxidative stress marker, i. e. malondialdehyde, glutathione, and superoxide dismutase, were estimated. Certain marker enzymes for different cell organelles, i. e. succinate and lactate dehydrogenases, glucose-6-phosphatase, acid phosphatase, and 5'-nucleotidase, were evaluated. The work was extended to determine the collagen content and the histopathological assessment of the stomach. Gastric ulcer exhibited a significant elevation of the ulcer index, antioxidant levels, collagen content, and the marker enzymes. The water extract attenuated these increments and was more potent as a protective agent, while the ethanol extract exhibited stronger therapeutic potency. In conclusion, A. speciosa acted as antiulcer agent. More detailed studies are required to identify the compounds responsible for the pharmacological effect.     

Gastroprotective effect of Terminalia arjuna bark on diclofenac sodium induced gastric ulcer

AIM: The present study was aimed to evaluate the effect of methanolic extract of Terminalia arjuna (TA) on diclofenac sodium induced gastric ulcer in experimental rats. METHODS: Animals were induced for gastric ulcer with diclofenac sodium (DIC) (80mg/kg bodyweight in water, orally) and treated orally with TA in various doses ranging from 100mg/kg bodyweight to 500mg/kg bodyweight. The effective dose was 400mg/kg bodyweight, since this dose elicited a maximum reduction in lesion index. The gastroprotective effect of TA was assessed from volume of gastric juice, pH, free and total acidity, pepsin concentration, acid output in gastric juice, the levels of non-protein sulfhydryls (NP-SH), lipid peroxide (LPO), reduced glutathione (GSH), and activities of enzymic antioxidants--super oxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), glutathione-S-transferase (GST) and myeloperoxidase (MPO) in gastric mucosa. The levels of DNA, protein bound carbohydrate complexes--hexose, hexoseamine, sialic acid, fucose in gastric mucosa and gastric juice and the levels of RNA in gastric mucosa were assessed. The stomach tissues were used for adherent mucus content and also for the histological examination. RESULTS: A significant reduction in lesion index was observed in ulcer induced animals treated with TA (DIC+TA) compared to ulcerated rats (DIC). A significant increase was observed in pH, NP-SH, GSH, enzymic antioxidants, protein bound carbohydrate complexes, adherent mucus content, nucleic acids with a significant decrease in volume of gastric juice, free and total acidity, pepsin concentration, acid output, LPO levels and MPO activities in DIC+TA rats compared to DIC rats. Histological studies confirmed the gastroprotective activity of TA. CONCLUSION: From the data presented in this study it could be concluded that T. arjuna acts as an gastroprotective agent probably due to its free radical scavenging activity and cytoprotective nature.     

Protective effect of quercetin on ischemia/reperfusion-induced gastric mucosal injury in rats.

This study was designed to determine the gastroprotective properties of quercetin in ischemia/reperfusion-induced gastric mucosal injury and the involvement of endogenous prostaglandins in this process. Oral pretreatment of rats with quercetin (100 mg x kg(-1)) 30 min before surgery significantly decreased the length of gastric mucosal lesions. However, lower doses of quercetin (25 and 50 mg x kg(-1)) only slightly decreased the gastric mucosal injury. Intraperitoneal application of indomethacin (5 mg x kg(-1)) had no effect in control (sham-operated) animals, but significantly worsened gastric injury in non-treated animals after ischemia/reperfusion. Furthermore, indomethacin only slightly reversed protective effect of quercetin. Non-treated animals showed a marked decrease in adherent mucus after ischemia/reperfusion. On the other hand, application of quercetin prevented this significant decrease even in animals pretreated with indomethacin. It can be concluded that antioxidant properties of quercetin and its mucus protective effect might be the main factors responsible for its protective effect against ischemia/reperfusion-induced gastric mucosal injury.     

Prevention of HCl-ethanol induced gastric mucosal injury in rats by Garcinia cambogia extract and its possible mechanism of action

Oral pretreatment of rats with G. cambogia fruit extract (1 g/kg body weight/day at interval of 7 and 15 days) protected gastric mucosa against HCl-ethanol induced damage by decreasing the volume and acidity of gastric juice. Increased lipid peroxidation, decreased activity of antioxidant enzymes, altered levels of protein and glycoproteins in the ulcerated mucosa, and gastric juice were maintained at near normal levels in G. cambogia pretreated rats. The results suggest the anti-ulcer activity of G. cambogia by virtue of its ability to decrease acidity and increase mucosal defense.     

木瓜药材HPLC指纹图谱研究

以熊果酸为参照物,利用高效液相色谱（HPLC）梯度洗脱,测定了19批木瓜（Chaenomeles speciosa（Sweet） Nakai）样品。建立了药用木瓜的高效液相指纹图谱,为评价控制药用木瓜的质量提供了依据。色谱柱为YPW—Kromasil TM—C18柱（250mm×4．6mm,5μm）（美国迪马公司）;流动相：甲醇（A）一1％冰醋酸（B）,流动相A为甲醇,流动相B为1％冰醋酸水溶液。检测波长290nm,柱温30℃,流速10mL／mjn,进样量20μL。通过分析19批木瓜样品得到的高效液相指纹图谱有11个共有峰,多数峰都可以达到较好分离且19批次相似度符合。因此药用木瓜的指纹图谱特征性及专属性强,可用于药用木瓜的质量控制。     

Gastroprotective effect of aparisthman, a diterpene isolated from Aparisthmium cordatum, on experimental gastric ulcer models in rats and mice.

Aparisthmium cordatum (Juss.) Bail. (Euphorbiaceae), known in the State of Pará, Brazil as "ariquena queimosa", is a medium-sized tree which is native to the North Brazilian coastal region. Previous phytochemical studies of the bark of A. cordatum yielded a furan diterpenoid with a clerodane skeleton, called aparisthman. Recently, we reported the antiulcerogenic activity of trans-dehydrocrotonin (DHC), a furan diterpene isolated from Croton cajucara bark, in different ulcerogenic models in mice and rats. The aim of the present study was to assess the possible antiulcerogenic activity of aparisthman. When previously administered (p.o.) at the dose of 100 mg/kg(-1), aparisthman reduced significantly (p < 0.01) gastric injury induced by the indomethacin/bethanechol (71%), ethanol (71%), pylorus ligature, (59%) and hypothermic restraint-stress models (50%), in mice and rats. In the HCl/ethanol-induced gastric ulcer model in mice, at oral doses of 100 and 250 mg/kg(-1), aparisthman from A. cordatum reduced significantly (p < 0.001) the formation of gastric lesions by 59% and 66%, respectively, as compared with control. In the pylorus-ligature model, aparisthman (p.o.) decreased the volume of gastric juice as compared with control (p < 0.001). When aparisthman (100 mg/kg(-1)) was administered intraduodenally to mice, significant modifications were found, such as a decrease in gastric acidity as compared with control. In the animals pre-treated with aparisthman, free mucus production increased by 19% in the gastric mucosa (p < 0.05). The results suggest that aparisthman from A. cordatum presents a significant anti-ulcer effect when assessed in these induced ulcer models. Although the mechanism underlying this antiulcerogenic effect remains unknown, it seems to be related to an increase of the defensive mechanisms of the stomach such as prostaglandin synthesis and mucus production. The good yield of aparisthman obtained from A. cordatum, as well as its antiulcerogenic activity, suggest that this compound should be submitted to pharmacological research as a potential new antiulcerogenic drug.     

The role of endogenous prostaglandins in the regulation of gastric secretion in rhesus monkeys

Prostaglandins (PG) are known to alter a variety of gastrointestinal functions, but the physiological role of endogenous PG remains unclear. This experiment was designed to evaluate changes in gastric secretion following both acute and chronic inhibition of PG synthesis with indomethacin (5 mg/kg s.c.). Gastric juice was collected by continuous aspiration in 8 conscious chair-adapted male rhesus monkeys following treatment with saline or indomethacin for one or four days. The gastric juice was analyzed for H+, Na+, K+, and Cl- concentrations. The amount of soluble mucus in the gastric juice was estimated using Alcian Blue dye binding of acidic glycoproteins and Periodic Acid Schiff reaction with neutral glycoproteins. PG levels were measured in the plasma and in biopsy samples of fundus, antrum and duodenum. Both one and four days of indomethacin significantly (p less than 0.05) decreased tissue PG levels in the fundus, antrum and duodenum. Plasma levels of PGF2 alpha were significantly (p less than 0.05) decreased after both one and four days of indomethacin, while PGE2 and 6-keto PGF1 alpha were significantly inhibited only after four days of indomethacin. Both acute and chronic inhibition of PG synthesis was accompanied by a decrease in the concentration of sodium and mucus in the gastric juice but by an increase in the output and concentration of hydrogen ion. These changes suggest a possible mechanism by which endogenous PG play a role in the regulation of gastric secretion and in the protection against gastrointestinal damage.     

皱皮木瓜果实中有机酸成分的GC-MS分析

木瓜为常用中药,其原植物为木瓜属（Chaenomeles Lindl．）植物皱皮木瓜[Chaenomeles speciosa（Sweet）Nakai],又称贴梗海棠、贴梗木瓜等,中国南方各地均有栽培。其花可供观赏;果实入药,能驱风强壮、舒筋、镇痛消肿,民间常用于浸泡药酒;种仁富含油酸和亚油酸。对皱皮木瓜有机酸的脂肪性成分已有研究报道,但尚无其水溶性有机酸成分的研究报道。皱皮木瓜的药用功效主要为镇痛解痉,但至今未明确其主效成分,有机酸虽具有一定的镇痛功效,但有机酸的种类不同,功效也不相同。鉴于长期以来皱皮木瓜还是作为汤药服用,所以就其水溶性有机酸组成及含量开展研究,特别是与其有机酸中的脂肪性成分进行比较研究尤为必要,对于揭示皱皮木瓜主要功效成分具有重要的意义。