Three new species of the genus Aporcelaimoides Heyns, 1965 from Vietnam (Nematoda,Dorylaimida, Aporcelaimidae), with an updated taxonomy of the genus

Three new species of Aporcelaimoides from natural habitats in Vietnam are studied, described and illustrated, including line drawings, LM and/or SEM pictures. Aporcelaimoides brevistylum sp. n. is characterized by its body 1.95–2.90 mm long, lip region offset by deep constriction and 17–18 µm broad, ventral side of mural odontostyle 11–14 µm long with aperture occupying 62–71% of its length, neck 663–767 µm long, pharyngeal expansion occupying 58–66% of total neck length, uterus a simple tube 85–182 µm long, pars refringens vaginae absent, V = 55–63, tail short and rounded (34–46 µm, c = 49–76, c’ = 0.6–0.8), spicules 67–86 µm long, and one ventromedian supplement out the range of spicules. Aporcelaimoides minor sp. n. is distinguished in having body 2.09–2.61 mm long, lip region offset by deep constriction and 19–20 µm broad, mural odontostyle 14–16 µm long at its ventral side with aperture occupying 73–84% of its length, neck 579–649 µm long, pharyngeal expansion occupying 57–66% of total neck length, uterus a simple tube 44–69 µm long, pars refringens vaginae well developed, V = 48–56, female tail very short, rounded conoid or truncate (14–26 µm, c = 90–146, c’ = 0.3–0.6), and male unknown. Aporcelaimoides silvaticum sp. n. is characterized by its body 2.09–2.60 mm long, lip region offset by depression and 17–18 µm broad, mural odontostyle 11–12 µm long at its ventral side with aperture occupying 60–66% of its length, neck 597–720 µm long, pharyngeal expansion occupying 58–64% of total neck length, uterus a simple tube 128–243 µm long, pars refringens vaginae well developed, V = 58–60, tail short and rounded (27–37 µm, c = 67–94, c’ = 0.6–0.7), spicules 64–75 µm long, and two or three widely spaced ventromedian supplements bearing hiatus. The genus Aporcelaimoides is restored, its diagnosis emended, and three species of Sectonema, namely Sectonema amazonicum, Sectonema haguei and Sectonema moderatum, transferred to it. An updated list of its species, a key to their identification and a tabular compendium with the most important morphometric features are also presented.     

Margollus bokanicus n. sp. from Iran,the Fourth Species of a Rare Nematode Genus (Dorylaimida,Tylencholaimellidae)

Margollus bokanicus n. sp., collected from natural habitats in Khorasaneh district, Bokan, West Azarbaijan province, Iran, is described. Morphological and morphometric data are provided as well as drawings and light microscopy illustrations. The new species is characterized by a medium size body length (0.60 to 0.73 mm), labial and postlabial sclerotizations, lip region 7-μm wide, offset by constriction and long neck (167 to 207 μm), long pharyngeal basal bulb (27 to 36 μm) or 16% to 17% of total neck length, female genital system monodelphic–opisthodelphic, anterior branch reduced to a uterine sac (26–29 μm) or 1.1 to 1.3 times the body diameter, long posterior uterus (25–28 μm) or 1.1 to 1.3 times the body diameter, V = 40 to 47, cylindroid female tail (17 to 24 μm, c = 31 to 38, c’ = 1.1 to 1.4), and males unknown. This taxon is easily distinguishable from other Margollus species by its smaller general size and more posterior vulva. A compendium of Margollus species is also presented.     

Longidorus ferrisi n. sp. from California Citrus

In October 1999, the authors received fixed specimens of a species of Longidorus from Howard Ferris found about the roots of a citrus tree in Oakville, Napa County, CA. After determining it to be new a species, we requested additional specimens. The samples contained roughly equal numbers of males and females. Longidorus ferrisi n. sp. is most similar to L. elongatus, but can be distinguished by a greater c-ratio (111-187 vs 73-141), a lesser c′ (0.7-1.1 vs 1.0-1.3), a more offset head, a more posterior guide ring (35-40 vs 30-33 μm), the presence of sperm in the uterus in mature females, and the approximate 1:1 ratio of females to males. Other similar species include L. artemisiae, L. crassus, L. glycines, and L. milanis. Longidorus ferrisi n. sp. differs from L. artemisiae by a lesser a-ratio (74-102 vs 109-155), a lesser c′ value (0.7-1.1 vs 1.0-1.6), a more posterior guide ring (35-40 vs 27-34 μm), a longer odontostyle (91-108 vs 84-98 μm), a wider lip region (16-19 vs 14-17 μm), wider mid-body (53-69 vs 41-52 μm), and longer spicules (57-65 vs 39-49 μm). The new species differs substantially from L. crassus by its lip shape and the presence of males, and differs from L. glycines by a shorter body (4.33-5.97 vs 6.14-8.31 mm), a lesser c′ value (0.7-1.1 vs 0.9-1.4), a narrower lip region (16-19 vs 20-23 μm), wider mid-body (53-69 vs 39-57 μm), longer spicules (53-69 vs 45-53 μm), and fewer supplements (7-11 vs 11-17). Longidorus ferrisi n. sp. differs from L. milanis by a longer body (4.33-5.97vs 3.00-4.90 mm), a greater c value (111-187 vs 86-130), a wider mid-body (53-69 vs 43-56 μm), a different head shape, and longer spicules (53-69 vs 41-54 μm). The nuclear 18S ribosomal DNA sequence of this species revealed that this species is unique with respect to all sequenced Longidorus species.     

Studies of the genus Enchodelus Thorne, 1939 (Nematoda, Nordiidae) from Arctic polar deserts. 1. Species with long odontostyle: E. makarovae sp. n. and E. groenlandicus (Ditlevsen, 1927) Thorne, 1939, with an identification key to the species of the E. macrodorus group

Two nematode species of the genus Enchodelus Thorne, 1939, one new and one known from Arctic polar deserts were studied. Enchodelus makarovaesp. n. is an amphimictic species, characterised by females with body length of 1.57-2.00 mm, lip region 15-17.5 μm wide, amphid duplex, odontostyle 38-43 μm long or 2.3-2.8 times lip region diam. Odontophore with flanges, 1.2-1.4 times as long as odontostyle; pharynx length 320-377 μm, pharyngeal expansion 113-130 μm long or 32-37% of total pharynx length; female genital system amphidelphic, uterus tripartite, pars refringens vaginae with two trapezoid sclerotisations, vulva a transverse slit (V=45-51%); tail bluntly conoid (25-35 μm, c=45.8-70.3, c'=0.6-0.9 in females, and 29-33 μm, c=46.4-58.9, c'=0.7-0.8 in males). Males with 65-74 μm long spicules and 10-12 spaced ventromedian supplements. Additional information for Enchodelus groenlandicus is provided, this being a new geographic record for the Putorana Plateau, Russian Arctic.     

Two New Species of Actinolaimidae Thorne, 1939 (Nemata: Dorylaimida) from China

Two new species of Actinolaimidae are described from China. Trachactinolaimus brevicaudatus n. sp. is 3.4-4.4 mm long; a = 40-60, b = 3.5-5.2, c = 20-21 in female and 34-39 in male; odontostyle length is 29-33 µm; spicules are 67-77 µm long; and the stoma has four onchia with numerous mural denticles. The female has a longitudinal vulva, and the male has 20 to 23 contiguous ventromedian supplements. Egtitus sinensis n. sp. is 1.7-2.2 mm long; a = 24-33, b = 3.1-3.9, c = 16-19 in female and 0.7-0.9 in male; odontostyle length is 25-29 µm; spicules are 55-56 µm long; and the prerectum 53-77 µm long. The cardia is short and blunt conoid, 13-19 µm long. The male has 12 to 13 ventromedian supplements at intervals of 2-3 µm.     

中国小无环咽属线虫一新纪录种(线虫纲,矛线目,无环咽科)

描述了采自山东省沂水县烟草Nicotiana tabacum Linn.根围土的中国线虫1新纪录种,钝尾小无环咽线虫Aporcelaimellus obtusicaudatus(Bastian,1865)Altherr,1968。该种主要的鉴别特征是雌虫虫体中等到大型,角质层由明显的2层组成;唇区角状,明显缢缩,唇瓣彼此分离;齿针强壮,长22.1(19.9～27.1)μm,其开口占齿针长的一半;食道矛线型,贲门圆锥形;雌虫双生殖腺,阴门通常位于体中部,内阴唇骨化明显,呈心形;内阴唇和阴道总长约占阴门处体宽的2/5;尾长31.9(23.2～37.3)μm,是肛门处体宽的0.7(0.6～0.8)倍,尾呈钝圆锥形到半球形,末端圆,有厚的肌肉层,肛门后有粗糙的放射状条纹。它与A.faridpuriensis的主要区别在于:齿针较短(19.9～27.1μm vs.35～39μm),前直肠较短(96.2μm vs.145μm)。它与A.krygeri的主要区别在于:齿针较短(22.1μm vs.25μm),尾较长(23.2～37.3μm vs.20～25μm),c’值大(0.6～0.8vs.0.2～0.3),尾呈钝圆锥形到半球形,A.krygeri尾非常短,半球形且背凹,具钉状末端(Singh,Sharma&Khatoon,2002;T horne,1974)。讨论了钝尾小无环咽线虫的已有报道和国内分布,对Aporcelaimellus属及相关属科的中文译名进行了厘订。     

Two New Species of Prismatolaimus de Man, 1880 (Nemata: Prismatolaimidae) in Southern Chile

Two new species of Prismatolaimus are described from Orange Bay, Hoste Island, Chile. Prismatolaimus novoporus sp. n. is distinguished by its females being 1.08-1.28 mm long and monovarial with a long postvulvar sac, and having a long tail (c'' = 21.5-32.2); males have 11-20 supplements confined to the posterior part of the body. Prismatolaimus chilensis sp. n. is distinguished by its amphidelphic reproductive system, short tail (c'' = 9.5), V = 63.4; males have 23 supplements reaching up to the neck region. A well-developed dorsal body pore behind the cardia connected with an apparently glandular organ is reported in P. novoporus sp. n. Function of the organ is unknown, and it is not a general feature in the genus. The generic diagnosis of Prismatolaimus is emended and keys to species, both females and males, are presented. The systematic position of Prismatolaimus is discussed, and it is judged to be the only genus of Prismatolaimidae. Also, it is concluded that Prismatolaimidae and Bastianidae represent two sister taxa in Araeolaimida or Leptolaimina.     

Longidorus carniolensis sp. n. (Nematoda, Longidoridae) from vineyard soil in Slovenia

A new needle nematode, Longidorus carniolensissp. n., recovered from the soil around the roots of grapevine Vitis vinifera L. from Slovenia, is described and illustrated. Longidorus carniolensisis an amphimictic species, characterised by females with a moderately long (L=5.6-8.2 mm) and plump (a=51-72.4, ave. 66.3) body, assuming a spiral to C-shape when heat relaxed. Head region continuous, anteriorly almost flat, lip region 23-25 μm wide; guiding ring situated posteriorly (42-47 μm, 43-50 μm in males), odontostyle long (ave. 146.6 (136-157) μm); pharyngeal glands with normal location, their nuclei of approximately equal size; tail bluntly conoidal to almost hemispherical. Males abundant, spicules slender and long (122-145 μm), ventromedian supplements 13-17, irregularly spaced, preceded by an adanal pair. Four juvenile stages present, the first stage juvenile with bluntly conoidal tail. Codes for identifying the new species when using the key by Chen et al. (1997) are: A 56, B 4, C 4, D 1, E 4, F 35, G 1, H 1, I 2. The new species is morphologically the most similar to Longidorus poessneckensis Altherr, 1974, Longidorus macrosoma Hooper, 1961, Longidorus caespiticola Hooper, 1961, Longidorus helveticus Lamberti et al., 2001, Longidorus macroteromucronatus Altherr, 1974, Longidorus pius Barsi & Lamberti, 2001, Longidorus raskii Lamberti & Agostinelli, 1993, Longidorus kheirii Pedram et al. 2008, Longidorus silvae Roca, 1993, Longidorus iuglandis Roca et al., 1985, Longidorus vinearum Bravo & Roca, 1995 and Longidorus major Roca & d'Erico, 1987, but differs from these species either by the body and odontostyle length, position of guide ring, head region and tail shape or the shape of the first stage juvenile tail. Sequence data from the D2-D3 region of the 28S rDNA distinguishes this new species from other speciesof the genus Longidorus with known sequences. Relationships of Longidorus carniolensissp. n. with other Longidorus species based on analysis of this DNA fragment and morphology are discussed.     

Detection of Babesia spp. in free-ranging Pukus, Kobus vardonii, on a game ranch in Zambia

Babesia spp. were detected from 4 asymptomatic pukus captured on a game ranch in central Zambia in October 2008. Blood smears were examined in 4 species of aymptomatic free-ranging antelopes, namely the puku (Kobus vordanii), reedbuck (Redunca arundinum), bushbuck (Tragelaphus sylvaticus), and kudu (Tragelaphus strepsiceros), and showed the presence of Babesia parasites only in the puku. In the puku, the prevalence of babesiosis was estimated at 33.3% (n = 12), while the overall prevalence in all examined animals was 8.5% (n = 47). The parasites showed morphological characteristics of paired ring-like stages with the length varying between 1.61 μm and 3.02 μm (mean = 2.12 μm, n = 27; SD = 0.76 μm). Both the infected and non-infected pukus showed good body condition scores (BCS), while the dominant tick species detected from all animals were Rhipicephalus appendiculatus, Rhipicephalus spp., and Boophilus spp. To our knowledge this is the first report of Babesia spp. infection in pukus in Zambia. These findings suggest that wildlife could play an important role in the epidemiology of babesiosis in Zambia.     

Steinernema akhursti sp. n. (Nematoda: Steinernematidae) from Yunnan, China

A new species of entomopathogenic nematode, herein described as Steinernema akhursti sp. n., was recovered from soil samples collected from Yunnan Province, the People's Republic of China. Both morphological and molecular data show congruently that S. akhursti sp. n. belongs to the Steinernema feltiae group. It can be separated from all described Steinernema species by the combined morphological and morphometrical characters of various stages of the nematodes. For the first generation male, the new species can be recognized by spicule length 90 +/- 4.6 microm, spicule tip blunt with an aperture on the ventral side, gubernaculum with a long and needle-shaped cuneus, and tail conoid with a prominent mucron on the tip and a concave on ventral side. For the infective juvenile, the combination of the following characters: body length 812 +/- 19 microm, distance from anterior end to excretory pore 59 +/- 1.5 microm, tail length 73 +/- 2.9 microm, E% 77 +/- 4.5, lateral field with six evenly distributed and identical ridges at the middle body portion, and tail with long and slightly constrict hyaline portion can be used to separate the new species from other nematodes. For the female, the new species is characterized by: tail conoid with a short mucron and slightly swelling anal portion and a symmetrical, slightly protruding vulva with conspicuous double-flapped epiptygma. The nematode can be separated from other described species of Steinernema by DNA sequences of either a partial 28S rDNA or the internal transcribed spacer regions of rDNA and from the closely related species S. feltiae and Steinernema oregonense by cross-breeding tests.     

Description of Globodera ellingtonae n. sp. (Nematoda: Heteroderidae) from Oregon

A new species of cyst nematode, Globodera ellingtonae, is described from soil collected from a field in Oregon. Second-stage juveniles (J2) of the species are characterized by body length of 365-515 μm, stylet length of 19-22.5 μm, basal knobs rounded posteriorly and pointed anteriorly, tail 39-55 μm, hyaline tail terminus 20-32.5 μm, and tail tapering uniformly but abruptly narrowing and constricted near the posterior third of the hyaline portion, ending with a peg-like, finely rounded to pointed terminus. Cysts are spherical to sub-spherical, dark to light brown and circumfenestrate and cyst wall pattern is ridge-like with heavy punctations. Males have a stylet length of 21-25 μm and spicule length of 30-37 μm with a pointed thorn-like tip. Females have a stylet length of 20-22.5 μm, one head annule and labial disc, heavy punctations on the cuticle, and short vulval slit 7.5-8 μm long. Morphologically this new, round-cyst species differs from the related species G. pallida, G. rostochiensis, G. tabacum complex and G. mexicana by its distinctive J2 tail, and by one or another of the following: shorter mean stylet length in J2, females and males; number of refractive bodies in the hyaline tail terminus of J2; cyst morphology including Granek’s ratio; number of cuticular ridges between the anus and vulva; and in the shape and length of spicules in males. Its relationship to these closely related species are discussed. Based upon analysis of ribosomal internal transcribed spacer (ITS) sequences, G. ellingtonae n. sp. is distinct from G. pallida, G. rostochiensis, G. tabacum and G. mexicana. Bayesian and Maximum Parsimony analysis of cloned ITS rRNA gene sequences indicated three clades, with intraspecific variability as high as 2.8%. In silico analysis revealed ITS restriction fragment length polymorphisms for enzymes Bsh 1236I, Hinf I, and Rsa I that overlap patterns for other Globodera species.     

中国滑刃属一新种记述(滑刃目,滑刃科)

2001～2002年从山东省青州市韭菜Allium tuberosum的根际土壤中发现滑刃属Aphelenchoides1新种,定名为韭菜滑刃线虫Aphelenchoides alliussp.nov.。该虫具有以下特征:虫体大型;唇区缢缩,口针较长,无口针基部球;侧区具有6条侧线;生殖系统发育良好,卵巢具1～2个转折,卵母细胞单列;尾细锥形,末端收缩呈细指状,平截,刺状尾尖突生于其上;雄虫常见,尾钩状,末端具刺状尾尖突,交合刺典型,顶尖和喙突明显。已报道种中有8个种与新种相似,其中5个种虫体体长较短,小于500μm,易与新种分开,它们是:细尖滑刃线虫A.conimucronatus(L=280～440μm),高头滑刃线虫A.editocaputis(L=270～320μm),高山滑刃线虫A.montanus(L=390～400μm),稀少滑刃线虫A.rarus(L=300～320μm),沃恩滑刃线虫A.vaughani(L=360～430μm)。另3个种与新种相比较:中心滑刃线虫A.centralis侧线4条,尾巴较长,雄虫不常见;陶比恰滑刃线虫A.daubichaensis侧线4条,口针较短且有小的基部球,后阴子宫囊较短,卵巢无转折;一点红滑刃线虫A.emiliae口针基部球发达,中食道球椭圆形,更大19μm×15μm,后阴子宫囊较短。     

Longidorus paravineacola n. sp. (Nematoda: Longidoridae), a New Species from Arkansas

Longidorus paravineacola n. sp., described herein, was found in a survey of longidorids of Arkansas. It is a parthenogeneticspecies characterized by its long body (6.68-9.85 mm); slightly expanded and rounded head, head width 21-27 µm; odontostyle length 95-114 µm; guide ring 28-37 µm posterior to the head end; short rounded tail, and c'' = 0.6-1.0. Longidorus paravineacola n. sp. is similar to the amphimictic species L. vineacola Sturhan &Weischer, 1964; L. balticus Brzeski, Peneva &Brown, 2000; L. kuiperi Brinkman, Loof &Barbez, 1987; and parthenogenetic species L. crassus Thorne, 1974, which also occurred in the type locality.     

Trichodorus elefjohnsoni n. sp. (Nemata: Trichodoridae) from Undisturbed Appalachian Forest

A new species of Trichodoridae, Trichodorus elefjohnsoni, is described from undisturbed regions of Great Smoky Mountains National Park, United States. It resembles T. orientalis De Waele &Hashim, 1984, T. persicus De Waele &Sturhan, 1987, and T. taylori De Waele, Mancini, Roca, '' Lamberti, 1982 in arrangement of ventromedian cervical papillae and posterior preanal supplements, but differs by combinations of the following characteristics: body length 516-731 μm; spicule length 33-50 μm, spicules densely striated, constricted medially; vaginal sclerotizations ovate; one pair of lateral body pores near vulva.     

Meloidogyne polycephannulata n. sp. (Nematoda: Meloidogynidae), a root-knot nematode parasitizing carrot in Brazil

Meloidogyne polycephannulata n. sp. is described from specimens collected from an area cultivated with carrot cv. Brasilia, in the city of Rio Paranaíba, in the region of Alto Paranaíba, Minas Gerais State, Brazil. The perineal pattern of the female is circular to ovoid with a high dorsal arch that has widely spaced, coarse annulations. The lateral field may have a deep furrow separating the dorsal and ventral arches. The medial lips are short and wide, whereas the lateral lips are large and triangular. The female stylet is 15-16 μm long with wide knobs, distinctly divided by an indentation in the center. Its tip is slightly curved dorsally. The excretory pore opens 34-65 μm from the anterior end. Females retain eggs and second-stage juveniles in their body cavity, similar to that of the cyst-forming nematodes. Males are 1.3-1.7 mm long and have a high head cap that is rounded and slopes posteriorly. The labial disc is fused to the medial lips. The head region has several irregular annulations that are similar in appearance to the first or second body annules that are likewise irregular, making the head region appear to be extremely large. The stylet of the male is 21-24 μm long; it is slender, and has small, rounded knobs, that are distinctly indented medially and appear heart-shaped. The shaft has several tiny projections throughout its length. Mean second-stage juvenile length is 411.7 μm. The juvenile head cap is elevated, the medial lips are small, and the lateral lips are elongate to triangular-shaped. The head region has several short, incomplete and irregular transverse annulations. The juvenile stylet is 14-23 μm long with small, rounded, and sloping knobs. The thin tail ends with a short hyaline portion that is variable in size (16-26 μm) and with a small, rounded tip. Isozyme profiles of esterases from Meloidogyne javanica show 3 strong bands (SB) at Rm 46, 59, and 66; profiles of M. polycephannulata n. sp. show a SB at Rm 47 and a weak band (WB) at Rm 52; M. petuniae has two SB at Rm 44 and 53; M. phaseoli has a SB at 53, 58, and 64 Rm; M. brasilensis has three SB at Rm 40, 58, and 66 and a WB at Rm 71; M. pisi has a SB at Rm 40, 60, and 64 and two WB at 46 and 50 Rm. Data from sequencing the 18S rDNA region of M. polycephannulata n. sp. confirms that it is different from M. arabicida, M. arenaria, M. ethiopica, M. incognita, M. javanica, M. paranaensis, and M. thailandica. Sequence identity among these eight species ranged between 85 to 93.4%. Meloidogyne polycephannulata n. sp. reproduces very well on carrot and tomato; poorly on pepper; and not at all on cotton, peanut, tobacco, watermelon, and sweet corn.     

中国黄海与中国东海管咽线虫属(线虫动物门)一新种与一新纪录

描述了中国黄海自由生活海洋线虫1新种Siphonolaimus boucheri sp.nov.,S.boucheri sp.nov.的主要特征是:口针短(21～23μm),头直径为10～11μm,圆形化感器直径为13μm,与相应体直径的比例为59%;短交接刺(61～65μm,为肛径的1.33倍),无交接辅器;尾巴圆锥形,雄性尾巴为肛径的3.57倍,雌性尾巴为肛径的4.58倍。同时,描述了中国东海管咽线虫科的1新纪录,Siphonolaimus profundus。模式标本存放于中国海洋大学生命学院。     

Nematodes of the Order Rhabditida from Andalucía Oriental,Spain. The Genera Nothacrobeles Allen &Noffsinger, 1971 and Zeldia Thorne, 1937

A new species of the genus Nothacrobeles is described from natural areas (a salt lake) in the Southeast Iberian Peninsula. Nothacrobeles lanceolatus sp. n. is characterized by its body length, two rows of cuticular punctations per annulus, labial probolae bifurcate with divergent prongs, pharyngeal corpus 2.4 to 3.5 times isthmus length, spermatheca length, postuterine sac 0.5 to 1.1 times the corresponding body diameter ratio, female tail conical and bearing a spindle-shaped or conical mucro with acute terminus, phasmid at 8 to 17 µm posterior to the anus, male tail conical with acute mucro, spicules length, and gubernaculum length. In addition, Nothacrobeles cf. lunensis and Zeldia punctata are studied. Cervidellus capricornis is transferred to genus Nothacrobeles. A key to species of Nothacrobeles is also provided.     

Xiphinema llanosum and Trophurus vultus, Two New Plant Nematode Species from Pasture Soils in Colombia

Xiphinema llanosum n. sp. and Trophurus vultus n. sp. are described and illustrated from grass soils in Llanos Orientales, Colombia. Xiphinema llanosum is a bisexual species. The female body length is 2.3-2.7 mm, odontostyle 86-96 μm, and odontophore 58-65 μm long; vulva at 42-47%; anterior ovary is absent; the anterior uterus and oviduct are similar to the posterior branch but slightly reduced; and the tail is dorsally convex-conoid with a blunt hemispherical terminus. Male body length is 2.06-2.96 mm; spicules are 40-44 μm long; and four (rarely three or five) anterior ventromedian supplementary papillae are present. Trophurus vultus females are 0.52-0.67 mm long; vulva at 56-60%; stylet is 10.5-13.5 μm long; isthmus is as long as the basal esophageal bulb; the tail is subclavate, 1.6-2.2 times anal body width long; and the terminal cuticle thickness is about one-sixth of the tail length.