In-depth Proteome of the Hypopharyngeal Glands of Honeybee Workers Reveals Highly Activated Protein and Energy Metabolism in Priming the Secretion of Royal Jelly

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Highlights

• •In-depth proteome underpins the gland ontogeny and age-specific activity of the HGs.
• •The well-developed acini in the HGs of NBs promote the RJ secretary activities.
• •The enhanced protein and energy metabolism in the HGs boost the stronger RJ secretion of RJBs.

     

Phytoplasma-induced Changes in the Acetylome and Succinylome of Paulownia tomentosa Provide Evidence for Involvement of Acetylated Proteins in Witches' Broom Disease

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Highlights

• •Quantitative global proteome, acetylome and succinylome of phytoplasma-infected Paulownia tomentosa seedlings.
• •Acetylation may be more important than succinylation in response to phytoplasma infection.
• •Acetylation modified the activities of POR and RuBisCO.
• •Possible model to elucidate the molecular mechanism responses to PaWB from proteome and PTMs.

     

ProAlanase is an Effective Alternative to Trypsin for Proteomics Applications and Disulfide Bond Mapping

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Highlights

• •ProAlanase is a powerful protease for efficient low pH disulfide bond mapping.
• •High suitability for analysis of histone family members and their PTMs.
• •Accurate phosphorylation profiling in proline-rich proteins.
• •Sequence coverage increase and full de novo sequencing in combination with trypsin.

     

Sucrose-induced Receptor Kinase 1 is Modulated by an Interacting Kinase with Short Extracellular Domain,

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Highlights

• •Functional role of a yet uncharacterized receptor kinase QSK1.
• •Activation model for SIRK1 receptor kinase in a heteromer with QSK1.
• •Role of QSK1 in substrate recruitment and stabilization of the complex.

     

林农复合生态系统的原理、特点及其类型

林农复合生态系统是根据生态位、生物种群之间共生、边缘效应、利用层、食物链“加环”和生态系统工程等原理建立起来的。林农复台生态系统依历史发展过程可分为原始林农复合系统、传统林农复会系统和现代林农复合系统;根据生产方式可分为“牧童”式、林农轮作式、林农间作式、食物网链式、树木菜园式、塘雅系统式和复合母系统式等７种类型;依复台种类及其用途来分,主要有林、农系统,林、牧系统,林、渔系统,林、渔、农系统。林、渔、牧系统,林、食用菌系统,林、水生作物、渔系统和林．副系统等８种类型。     

Spatiotemporal Changes of the Phagosomal Proteome in Dendritic Cells in Response to LPS Stimulation*

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Highlights

• •Characterization of the phagosomal proteome comparing resting and LPS-treated BMDCs.
• •Label-free quantification determined 2843 phagosomal proteins.
• •Reduced recruitment of hydrolases and V-ATPase to phagosomes of LPS-treated cells.
• •Increased recruitment of antigen cross-presentation molecules to these phagosomes.

     

Proteomics of Asrij Perturbation in Drosophila Lymph Glands for Identification of New Regulators of Hematopoiesis

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Highlights

• •First report on the quantitative proteomic profiling of Drosophila lymph glands.
• •Comparative proteomic analysis under conditions of perturbed blood cell homeostasis.
• •Resource for identifying new regulators of insect and vertebrate hematopoiesis.

     

Glycoproteogenomics: A Frequent Gene Polymorphism Affects the Glycosylation Pattern of the Human Serum Fetuin/α-2-HS-Glycoprotein

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Highlights

• •Frequent genetic polymorphism affects the glycosylation pattern of fetuin.
• •Personalized in-depth proteoform profiling of fetuin purified from 20 donors.
• •Classification of serum donors into three different genotypes.
• •Septic patients show increased level of fucosylation at N-glycolation site N176.

     

Integration of Two In-depth Quantitative Proteomics Approaches Determines the Kallikrein-related Peptidase 7 (KLK7) Degradome in Ovarian Cancer Cell Secretome

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Highlights

• •Kallikrein-related peptidase 7 is over expressed in ovarian cancer.
• •Quantitative PROTOMAP and TAILS approaches identified putative substrates of KLK7.
• •Pro-MMP10 is activated by KLK7.
• •KLK7 cleaves thrombospondin 1 and IGFBP6 in vitro.

     

Posttranslational Modifications Drive Protein Stability to Control the Dynamic Beer Brewing Proteome

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Highlights

• •SWATH-MS profiles protein abundance and modification during mashing in beer brewing.
• •Proteolysis due to barley proteases leads to extreme proteoform diversity.
• •Sequence-specific proteolytic clipping of proteins controls their stability.

     

Regulated Phosphosignaling Associated with Breast Cancer Subtypes and Druggability,

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Highlights

• •Our search identifies 2,134 kinase-substrate phosphosite pairs in breast cancer.
• •CDKs and MAPKs are dominant regulators of trans substrate-phosphorylation.
• •Druggability, outcomes, and immune signatures related to kinase-substrates.
• •Experimentally validated activated phosphosites of ERBB2, EIF4EBP1, and EGFR.

     

Cathepsin L Regulates Metabolic Networks Controlling Rapid Cell Growth and Proliferation

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Highlights

• •Cts L^−/− cells proliferate faster than wild types and shift to aerobic glycolysis.
• •LDHA is a key glycolytic enzyme controlling Cts L^−/− cell proliferation.
• •Cts L regulates LDHA expression and function.

     

Unraveling Hidden Components of the Chloroplast Envelope Proteome: Opportunities and Limits of Better MS Sensitivity

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Highlights

• •Identification of previously undetected chloroplast envelope proteins.
• •Up to date manual annotation of genuine (or shared) envelope components.
• •New hypotheses for localizations, functions, interactions among cell compartments.
• •A new resource of significant value to the broader plant science community.

     

Reductive Stress Selectively Disrupts Collagen Homeostasis and Modifies Growth Factor-independent Signaling Through the MAPK/Akt Pathway in Human Dermal Fibroblasts

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Highlights

• •Changes to the proteome of skin fibroblasts subjected to reductive stress have been quantitated.
• •Only a small set of proteins is selectively diminished upon exposure to reductants.
• •Collagens (COL1A2 and COL6A2) emerge as sentinels of reductive stress.
• •Reductive stress triggers receptor-independent Akt phosphorylation at Ser473.