共查询到20条相似文献,搜索用时 781 毫秒
1.
Jack William Houghton Guy Carpenter Joachim Hans Manuel Pesaro Steven Lynham Gordon Proctor 《Molecular & cellular proteomics : MCP》2020,19(10):1664-1676
Highlights
- •Salivary secretion was increased by mouth rinsing with TRP channel agonists.
- •The salivary proteome varied over time and was changed by TRP channel stimulation.
- •Immunoreactive Cystatin S was increased in saliva after TRPV1 stimulation.
2.
Barbara Steigenberger Henk W.P. van den Toorn Emiel Bijl Jean-Franois Greisch Oliver Rther Markus Lubeck Roland J. Pieters Albert J.R. Heck Richard A. Scheltema 《Molecular & cellular proteomics : MCP》2020,19(10):1677-1687
Highlights
- •Cross-linked peptides are physically separated from mono-linked peptides in the gas-phase by TIMS ion mobility.
- •Development of a novel data acquisition routine that a-priori distinguishes cross-linked from mono-linked peptides called caps-PASEF.
- •First application of PhoX-driven cross-linking mass spectrometry on the timsTOF Pro.
- •Application of cross-linking mass spectrometry to medium to high complexity samples.
3.
《Molecular & cellular proteomics : MCP》2020,19(4):690-700
Highlights
- •Two molecular groups in anal squamous carcinoma according proteomic profile.
- •Differences in possible targeted processes such as metabolism or immune response.
- •Different percentage of tumor lymphocyte infiltration.
- •Difference in the frequency of ATM variants, related to PPAR inhibitors.
4.
Fei Fang Qun Zhao Huiying Chu Mingwei Liu Baofeng Zhao Zhen Liang Lihua Zhang Guohui Li Liming Wang Jun Qin Yukui Zhang 《Molecular & cellular proteomics : MCP》2020,19(10):1724-1737
Highlights
- •Mechanistic insights into ionic liquids and proteins at molecular level.
- •Extractants prescreen for proteome analysis with MD simulation system.
- •A loss-less sample preparation method developed for in-depth proteome profiling.
- •Over 3,300 proteins were confidently identified from 1,000 HeLa cells in a 1 h run.
- •Label-free quantitative proteome analysis of human liver cancer tissues.
5.
6.
Yadong Yu Haichuan Liu Zanlin Yu H. Ewa Witkowska Yifan Cheng 《Molecular & cellular proteomics : MCP》2020,19(12):1997-2015
Highlights
- •All six binding sites in PANWT are occupied by ADP- or ATP-type nucleotides.
- •PANKA Walker A mutant substoichiometrically binds ATP- but not ADP-type nucleotides.
- •PAN hexamer dissociation of the solution origin characteristics was observed in MS.
- •We posit that the PAN hexamer dissociation proceeds within the ESI droplets.
7.
《Molecular & cellular proteomics : MCP》2020,19(3):501-517
Highlights
- •Urinary peptide profiling of youths with type 1 diabetes before clinical injury.
- •Internal validation of uromodulin peptides by parallel reaction monitoring.
- •Discovery of novel bioactivity of uromodulin peptides in vitro.
- •In silico prediction of proteases involved in uromodulin processing.
8.
《Molecular & cellular proteomics : MCP》2020,19(11):1876-1895
Highlights
- •Guidelines for studying protein complexes via co-fractionation mass spectrometry.
- •A novel procedure for profiling gold standard protein complexes in CF-MS data.
- •Recommendations for efficient CF-MS fractionation collection.
- •Scoring metric recommendations for precise and sensitive CF-MS data analysis.
9.
Mark K. Adams Charles A.S. Banks Janet L. Thornton Cassandra G. Kempf Ying Zhang Sayem Miah Yan Hao Mihaela E. Sardiu Maxime Killer Gaye L. Hattem Alexis Murray Maria L. Katt Laurence Florens Michael P. Washburn 《Molecular & cellular proteomics : MCP》2020,19(9):1468-1484
Highlights
- •Sin3 paralog identity influences Sin3 complex composition.
- •Chemical cross-linking mass spectrometry identifies domains in SIN3A and SIN3B that mediate complex formation.
- •Complex subunit homology to yeast Sin3 complex components may assist in defining distinct forms of the Sin3 complex in humans.
- •A nuclear import signal within SIN3B is identified via chemical cross-linking mass spectrometry.
10.
《Molecular & cellular proteomics : MCP》2020,19(5):900-912
Highlights
- •Affinity-based proteomics of infected macrophage cells.
- •Salmonella-modified membranes exhibit host-specific composition.
- •Proteome differences explain some host-dependent pathophysiological differences.
11.
《Molecular & cellular proteomics : MCP》2020,19(11):1739-1748
Highlights
- •Label-free and isobaric labeling approaches for in-depth profiling of single cells.
- •Miniaturization and simplification of sample processing reduce surface losses.
- •Nanoflow separations enhance ionization efficiency and reduced chemical noise.
- •Ultrasensitive mass spectrometry and gas-phase separation add selectivity.
12.
Prashali Bansal Johannes Madlung Kristina Schaaf Boris Macek Fulvia Bono 《Molecular & cellular proteomics : MCP》2020,19(9):1485-1502
Highlights
- •Label-free and dimethyl labeling MS analysis of 6 RBPs from Drosophila ovaries.
- •Functionally related RBPs show overlapping proteomes.
- •Selective co-purification of splicing factors and translational regulators.
- •Validation of 26 novel interactions by co-immunoprecipitation.
13.
Tirsa L. E. van Westering Henrik J. Johansson Britt Hanson Anna M. L. Coenen-Stass Yulia Lomonosova Jun Tanihata Norio Motohashi Toshifumi Yokota Shin'ichi Takeda Janne Lehti Matthew J. A. Wood Samir EL Andaloussi Yoshitsugu Aoki Thomas C. Roberts 《Molecular & cellular proteomics : MCP》2020,19(12):2047-2068
Highlights
- •Proteomics analysis was performed in two murine models of Duchenne muscular dystrophy (mdx and mdx52) at three ages (8, 16 and 80 weeks) and compared with wild-type controls.
- •High-resolution isoelectric focusing liquid chromatography-tandem mass spectrometry enabled the quantification of 4974 proteins in all samples.
- •This study has revealed protein signatures of dystrophin deficiency and the progression of dystrophic pathology.
- •In contrast, the proteomes of the mdx and mdx52 mice were highly similar.
- •Pathway analysis revealed crosstalk between inflammatory, metabolic and muscle growth processes in dystrophic muscle.
14.
Elez D. Vainer Juliane Kania-Almog Ghadeer Zatara Yishai Levin Gilad W. Vainer 《Molecular & cellular proteomics : MCP》2020,19(10):1619-1631
Highlights
- •TOP: robust, bio-friendly FFPE proteome extraction method with less fixation bias.
- •Proteome of MSI-H colorectal cancer identifies immunobiology key elements.
- •MSI-H tumor displays an “INFg-STAT1 centric signature”.
- •Long-term IFNg induction In-vitro mimicks MSI-H signature.
15.
Felicia Grasso Stefania Mochi Federica Fratini Anna Olivieri Chiara Curr Inga Siden Kiamos Elena Deligianni Cecilia Birago Leonardo Picci Elisabetta Pizzi Tomasino Pace Marta Ponzi 《Molecular & cellular proteomics : MCP》2020,19(12):1986-1997
Highlights
- •Quantitative analysis of Plasmodium sexual stage egress secretome.
- •Activated gametocytes release gender-related proteins.
- •Gametocyte egress process involves different types of vesicles.
16.
《Molecular & cellular proteomics : MCP》2020,19(6):1058-1069
Highlights
- •Highly parallelizable 4D feature detection in ion mobility enhanced shotgun proteomics.
- •Multidimensional non-linear mass, retention time and ion mobility recalibration.
- •Collision cross section aware matching between runs.
- •Label-free quantification of ion mobility MS data.
17.
18.
《Molecular & cellular proteomics : MCP》2020,19(7):1193-1208
Highlights
- •cGAS acetylations and phosphorylations under basal and immune-stimulated states.
- •K384 and K414 acetylations and S305 phosphorylation inhibit cGAS-mediated apoptosis.
- •Acetylation at K198 stimulates cGAS-dependent interferon signaling.
- •K198 acetylation is decreased upon herpesvirus infection.
19.
《Molecular & cellular proteomics : MCP》2020,19(5):884-899
Highlights
- •pY phosphoproteomes and dedicated ranking analyses for 16 AML cell lines.
- •RTK drivers, 6 mutant cell lines confirmed, identification for 4 more cell lines.
- •MAPK1/3 phosphorylation for cell lines without TK driver, indicating RAS mutation.
- •Drug target space phosphorylation correlates with drug IC50s in specific cell lines.
20.
《Molecular & cellular proteomics : MCP》2020,19(2):405-420
Highlights
- •Analysis of product ions produced by 213 nm UVPD is used to refine database search.
- •A product ion at the N-terminus of Pro, y-2, is observed in 213 nm UVPD spectra.
- •213 nm UVPD provides more complete proteoform characterization than HCD.
- •HCD and 213 nm UVPD are complementary fragmentation methods for proteoforms <30 kDa.