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使用ELISA技术检测中草药抗HBeAg的实验研究 总被引:12,自引:1,他引:11
使用ELISA技术对250种中草药水提取物进行抗HBeAg的实验研究,共筛出有效药物7种(占总数的2.8%)。若按5种不同剂量(0.3、0.6、1.2、2.5、5.0mg/100μl)的药物、2种不同浓度的HBeAg(10.92、14.26P/N值)与3种不同接触时间(立即、1h、2h)的10项P/N值均数来综合评价药效指数时,7种有效药物的次序为丹参(1.55)、皂夹(2.34)、黄莲(2.81 相似文献
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香石竹的叶片培养及植株再生 总被引:14,自引:0,他引:14
1植物名称香石竹(Dianthuscaryophy-llus),别名康乃馨。2材料类别无菌苗叶片。3培养条件以MS为基本培养基。分化培养基附加:(1)6-BA1.0mp·L-1(单位下同)+NAA0.3;(2)6-BA1.0+NAA0.1;(3)6-BA1.0+NAA0.05。增殖培养基附加6-BA0.5+NAA0.1。分化和增殖培养基均加蔗糖3%、琼脂0.7%,pH5.8。生根培养基为1/2MS附加NAA0.1,蔗糖2%,琼脂0.6%,pH5.8。培养温度为(25±1)℃,光照12h·d-1,… 相似文献
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本文对出生后接种不同剂量乙肝血源疫苗的272名婴儿进行了3-5年的效果随访观察。结果表明,接种3、4、5年后,抗-HBs阳性率仍分别保持在90.5%、82.3%和73.2%,HBsAg阳性率分别为2.8%、3.1%和4.2%。3年后抗-HBs阳性率逐年下降,P/N值>50者以接种后3年为多,<50者以5年为多,3年后也呈下降趋势。随访结果说明,接种10μg×4、5μg×4及2.5μg×4剂量的乙肝疫苗具有阻断母婴乙肝病毒传播的效果。鉴于P/N值及抗-HBs阳性率在接种后3年开始下降,建议接种后3年应进行一次加强接种。 相似文献
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蚯蚓体内一种纤溶酶原激活剂(e-PA)对ATEE的降解 总被引:3,自引:0,他引:3
赤子爱胜蚓(Eiseniafetida)体内的一种纤溶酶原激活剂(e-PA)能够降解人工合成底物N-乙酰-L-酪氨酸乙酯(ATEE),该降解反应的最适pH为8.5,而且在0.2mol/LNa2HPO4中的活性要强于在0.05mol/LTris-HCl(pH8.5)中.分别测定了e-PA的大小亚基及全酶在0.2mol/LNa2HPO4与0.05mol/LTris-HCl(pH8.5)两种体系中的Km和Kcat.结果表明,在0.2mol/LNa2HPO4中,全酶的ATEE活性远远高于大小亚基单独的ATEE活性,而在0.05mol/LTris-HCl(pH8.5)中则没有这种现象.从蛋白质结构的角度对这一结果作了解释.用不同抑制剂和e-PA作用,结果表明,pepstatin,E-64和EDTA对e-PA的ATEE活性都有不同程度的抑制,这一点与e-PA的BAEE活性不同. 相似文献
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宫粉郁金的组织培养和快速繁殖 总被引:8,自引:1,他引:7
1植物名称宫粉郁金(Curcuma kwangsiensis)。2材料类别 块茎萌动芽。3培养条件 萌动芽生长培养基:(1)MS+6-BA1mg·L-1(单位下同)+NAA0.2。不定芽增殖与愈伤组织诱导培养基:(2)MS+6-BA10+KT5;(3)MS+6.BA10;(4)MS+6-BA5+KT2.5;(5)MS+6-BA5;(6)MS+6-BA2+KT1。生根培养基:(7)MS+NAA0.5;(8)MS+6-BA0.5+NAA0.5;(9)MS。以上培养基均加0.7%琼脂,3%蔗糖,pH5… 相似文献
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深红蔓绿绒的组织培养与快速繁殖 总被引:3,自引:0,他引:3
1 植物名称 深红蔓绿绒(Philodendronmandaianum“RoyalQueen”)。2 材料类别 顶芽、侧芽。3 培养条件 以MS为基本培养基。(1)芽体启动培养基:MS+6BA2mg·L-1(单位下同)+NAA0.01;(2)不定芽增殖培养基:MS+6BA3~4+NAA0.1;(3)生根培养基:1/2MS+6BA0.5+IBA2。以上培养基均加琼脂7.5g·L-1,pH值为5.8,培养室温度(26±2)℃,光照度1000lx,每日光照时间12h。培养基(1)与(2)中蔗糖用… 相似文献
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玉香梨的组织培养与快速繁殖 总被引:18,自引:0,他引:18
1植物名称沙梨品种玉香梨(Pyruspyrifolia cv. yuxiang)。2材料类别侧芽。3培养条件(1)丛芽诱导培养基:1/2MS大量元素+MS微量元素+铁盐+有机成分+6-BA2mg·L-1(单位下同)+NAA0.2+GA32+3%蔗糖+0.8%琼脂,固体培养;(2)增殖培养基:1/2MS+BA1.5+NAA0.2+GA31+3%蔗糖+0.8%琼脂,固体培养;(3)生根培养基:1/2MS+IBA10+1.5%蔗糖+0.6%琼脂,固体培养8d后转入不含任何激素的同类培养基中的二步生根法… 相似文献
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紫菀花序芽培养及植株再生 总被引:1,自引:0,他引:1
1植物名称紫菀(Astertataricus)。2材料类别花序芽。3培养条件愈伤组织、不定芽诱导及增殖培养基:(1)MS+6-BA0.5~2.0mg·L-1(单位下同);生根培养基:(2)MS+NAA0.5~2.0+6-BA0.2,(3)1/2MS(大量元素减半)+NAA0.5~2.0+6-BA0.2。上述培养基均加0.8%琼脂和3%蔗糖,高温高压灭菌前pH值调至5.8。培养温度为(2312)℃,每天光照12h,光照度为1500~2000lx4生长与分化情况4.1愈伤组织及不定芽的诱导剪取长约0… 相似文献
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Curcumin is the yellow pigment of turmeric that interacts irreversibly forming an adduct with thioredoxin reductase (TrxR), an enzyme
responsible for redox control of cell and defence against oxidative stress. Docking at both the active sites of TrxR was performed to compare
the potency of three naturally occurring curcuminoids, namely curcumin, demethoxy curcumin and bis-demethoxy curcumin. Results show
that active sites of TrxR occur at the junction of E and F chains. Volume and area of both cavities is predicted. It has been concluded by
distance mapping of the most active conformations that Se atom of catalytic residue SeCYS498, is at a distance of 3.56 from C13 of
demethoxy curcumin at the E chain active site, whereas C13 carbon atom forms adduct with Se atom of SeCys 498. We report that at least
one methoxy group in curcuminoids is necessary for interation with catalytic residues of thioredoxin. Pharmacophore of both active sites of
the TrxR receptor for curcumin and demethoxy curcumin molecules has been drawn and proposed for design and synthesis of most probable
potent antiproliferative synthetic drugs. 相似文献
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正Dear Editor,In December 2019, a novel human coronavirus caused an epidemic of severe pneumonia(Coronavirus Disease 2019,COVID-19) in Wuhan, Hubei, China(Wu et al. 2020; Zhu et al. 2020). So far, this virus has spread to all areas of China and even to other countries. The epidemic has caused 67,102 confirmed infections with 1526 fatal cases 相似文献
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The young pistils in the melanthioid tribes, Hewardieae, Petrosavieae and Tricyrteae, are uniformly tricarpellate and syncarpous. They lack raphide idioblasts. All are multiovulate, with bitegmic ovules. The Petrosavieae are marked by the presence of septal glands and incomplete syncarpy. Tepals and stamens adhere to the ovary in the Hewardieae and the Petrosavieae but not in the Tricyrteae. Two vascular bundles occur in the stamens of the Hewartlieae and Tricyrtis latifolia. Ventral bundles in the upper part of the ovary of the Hewardieae are continuous with compound septal bundles and placental bundles in the lower part. Putative ventral bundles occur in the alternate position in the Tricyrteae and putative placental bundles in the opposite. position in the Petrosavieae. The dichtomously branched stigma in each carpel of the Tricyrteae is supplied by a bifurcated dorsal bundle. 相似文献
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鸡传染性法氏囊病病毒研究进展 总被引:3,自引:0,他引:3
传染性法氏囊病(Infection bursal disease, IBD)是由鸡传染性法氏囊病毒(Infectious bursal disease virus, IBDV)引起的鸡和火鸡的一种高度接触性传染病,给世界各国的禽养殖业带来了巨大损失.自IBDV发现至今新的变异株不断出现,分子结构的改变导致病毒致病力的改变及宿主对疫苗应答的改变,使得传统的疫苗已不能控制其流行,因此各国学者对其基因组结构和功能进行了广泛深入的研究,并积极研制新型有效的疫苗以达到防治的目的. 相似文献
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Renfei Lu Xiuming Wu Zhenzhou Wan Yingxue Li Lulu Zuo Jianru Qin Xia Jin Chiyu Zhang 《中国病毒学》2020,35(3):344-347
In conclusion, the novel visual RT-LAMP assay is a simple, rapid, and sensitive approach for detection of SARS-CoV-2, and it is ready for application in primary care and community hospitals or health care centers, and even patients' own houses in response to the current SARS-CoV-2 epidemic because the assay does not require sophisticated equipment and skilled personnel. Furthermore, it is also ready to be used in fields for screening samples from wild animals and environments to facilitate the identification of potential intermediate hosts that mediate the cross-species transmission of SARS-CoV-2 from bats to humans. 相似文献
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Shen Jia-Yuan Li Man Xie Lyu Mao Jia-Rong Zhou Hong-Ning Wang Pei-Gang Jiang Jin-Yong An Jing 《中国病毒学》2021,36(1):145-148
正Dear Editor,Chikungunya virus (CHIKV), an arbovirus in the family of Togaviridae, genus Alphavirus, is transmitted by the A.aegyptii or A. albopictus mosquito, and causes disease in humans characterized by fever, rash, and arthralgia (Silva and Dermody 2017; Suhrbier 2019). It was first reported in 1953 in Tanzania, and caused only a few outbreaks and sporadic cases in Africa and Asia in last century. However, in the epidemic in 2004, CHIKV acquired mutations that conferred enhanced transmission by the A. albopictus mosquito(Schuffenecker et al. 2006). Since then, it has successively caused outbreaks in Africa, the Indian Ocean, South East Asia, the South America, and Europe (Zeller et al. 2016). 相似文献