首页 | 本学科首页   官方微博 | 高级检索  
相似文献
 共查询到20条相似文献,搜索用时 115 毫秒
1.
【目的】本研究旨在探究光周期对小菜蛾Plutella xylostella(Linn.)羽化节律的调控作用,为小菜蛾的季节性种群动态预测提供理论基础。【方法】在自然光周期和逆转光周期条件下,观察小菜蛾每日的羽化情况。【结果】在自然光周期和逆转光周期条件下,小菜蛾蛹在24 h均可以羽化。正常光周期下,羽化行为表现为近似抛物线形昼夜节律,雌成虫通常在黑暗阶段羽化(18:00-6:00),而雄成虫大多在光照阶段羽化(6:00-18:00)。在逆转光周期下,小菜蛾蛹的羽化无明显规律。在正常光周期下,雌成虫的羽化频率在光期和暗期没有显著差异,但是,相比于暗期,雄成虫在光期羽化率较高;在逆转光周期后,雌成虫在暗期(6:00-18:00)的羽化数量比光期(18:00-6:00)高,雄成虫暗期的羽化数量比光期低。【结论】光周期逆转后小菜蛾雌成虫和雄成虫的羽化节律明显改变,这将有助于阐明害虫光周期变化与生物钟之间的关系。  相似文献   

2.
【目的】掌握区域小菜蛾Plutella xylostella(L.)田间种群发生消长规律及温度、降雨和种植制度模式对种群的影响。【方法】2009—2014年,采用性诱剂监测和田间系统调查方法连续6年对滇中菜区——通海县小菜蛾田间种群数量开展成虫和幼虫田间种群数量的研究,分析当地月平均气温、月降雨量和种植模式因素对小菜蛾种群数量的相关性。【结果】(1)通海小菜蛾成虫种群消长年度间波动差异明显,全年呈现1个大的虫峰期为2—11月份,年度间出现小的2~3个虫峰。2012年种群数量较高,高峰期提前到4月份,与2013年高峰期的7—8月份差异明显,2010年和2011年田间种群数量是历年较少年份,种群高峰期的虫量少于百头/盆(卡)/月;(2)小菜蛾幼虫种群全年有2~3个危害峰期,3—6月是发生主峰期,9—11月是弱峰期;与成虫高峰期比较,幼虫田间种群,高峰期推迟20~30 d左右,全年的波动消长情况基本一致;(3)小菜蛾成虫与温度显著相关r=0.442,与降雨量成正相关r=0.283,但相关程度弱;幼虫种群与温度显著相关r=0.530,相关程度显著,与降雨没有相关性r=0.125,相关程度微弱;幼虫种群与成虫有相关性r=0.253,但相关程度微弱。【结论】滇中菜区常年成片种植十字花科蔬菜,一年4造、周年复种的种植制度对小菜蛾的种群发生有促进作用,区域模式相对稳定是造成小菜蛾种群周年活动频繁危害严重的主要原因之一。  相似文献   

3.
本研究监测了不同生境下瓜实蝇雄成虫的数量动态,分析了气象因子与其数量变动的相关性。结果表明儋州地区瓜实蝇雄成虫发生低谷为每年12月和1月;从2月开始,瓜实蝇雄成虫数量逐渐增加,4-8月达到高峰,从9月开始数量逐渐下降。生境显著影响了瓜实蝇的雄成虫数量,混合菜地、园林区以及混合果园诱集的瓜实蝇雄虫数量多于住宅区、农贸市场、苦瓜地诱集的雄成虫数量。气象因子也显著影响了瓜实蝇雄虫的数量。经逐步回归分析发现瓜实蝇雄虫数量变动受月降水量、月均最高气温、月均最小湿度综合影响,其中月均最高气温是影响瓜实蝇雄虫数量变动的最重要因素。因此,瓜实蝇防控应不仅局限在其寄主园区生境,还应根据其监测的数据对多生境的瓜实蝇进行治理。  相似文献   

4.
【目的】明确北京北部山区粘虫Mythimna separata(Walker)的种群动态和扑灯规律,探索粘虫的早期监测预警技术。【方法】2011年,在北京延庆利用时控开关控制探照灯诱虫器对粘虫的种群动态进行监测,并对整夜诱虫数量自动分时段取样。获得逐日种群动态数据和相关的生物学参数,使用Grads分析粘虫数量与空中风场的关系。【结果】全年诱集粘虫蛾9 699头,单日诱蛾最高1 366头。全年诱蛾可分为3个世代,各个世代种群均由多个高峰组成,并非陆续增加。性比分析发现雌虫数量显著多于雄虫。分时段数据显示粘虫整夜的扑灯节律可分为迁入型、迁出型或本地繁殖型、过境型和迁入迁出混合型。风场分析表明,诱虫高峰与空中风场偏南气流的频率与持续时间高度一致。【结论】北京延庆全年发生粘虫3个世代,二代幼虫造成的危害最大。一代和二代诱蛾数与空中是否存在偏南气流密切相关。雌虫可能具有更强的迁飞能力。对粘虫扑灯节律数据深入分析,可为迁飞种群是否在本地降落繁殖提供参考依据。  相似文献   

5.
【目的】小菜蛾Plutella xylostella是主要为害十字花科作物的一种世界性害虫,强大的生殖能力是其成为田间最难防治的害虫的主要原因之一。交配是营两性生殖昆虫繁衍后代必要的一个生理过程,明确小菜蛾雌雄成虫的交配行为及交配后的生理响应,对于小菜蛾种群监测和综合防治具有重要意义。【方法】解剖观察小菜蛾成虫生殖腺及精包形成;利用行为学与生物学实验,测定和分析小菜蛾成虫交配与再交配能力、精包形成与消化,以及交配次数对精包形成以及雌虫生殖力的影响。【结果】小菜蛾雄成虫在交配过程中精液以精包的形式传递给雌成虫,在交配囊中精包呈白色、不透明、气球状结构,交配结束后精包可被充分消化和吸收。成虫交配能力观测结果显示,小菜蛾雌雄成虫均具有多次交配行为。首次交配后,雄成虫表现出短暂的再交配延迟,20 min内其交配成功率为54.6%,显著低于首次交配。虽然交配次数不会影响雄成虫的交配时长,但雄成虫交配史对其自身的精包大小以及雌成虫的生殖力均具有显著的抑制作用。雌成虫表现出明显的再交配抑制性,交配过的雌成虫在12 h内的交配率显著低于未交配雌成虫的,这可能取决于首次交配后精包的消化和吸收速率。雌成虫多次交配后,其产卵量和卵孵化率与单次交配的相比没有显著差异。【结论】多次交配会使小菜蛾雄成虫再交配延迟,雌成虫再交配受到抑制;多次交配产生的雄成虫精包显著减小,雌成虫产卵量与卵孵化率并没有从多次交配中获得收益。本研究为解析小菜蛾雌雄成虫生殖调控机制奠定理论基础。  相似文献   

6.
昆虫性信息素的应用为有害昆虫的防治开辟了一条新的途径。它具有专一性强、灵敏度高、无毒、无公害、安全高效、使用方便等优点,在生产中显示出广泛的应用前景。一方面它可直接消灭害虫(诱杀雄虫、阻断交配、降低繁殖率等);另一方面还可用来观测害虫的发生动态及数量消长规律,为化学防治提供科学依据。但是,目前发现某些文献上将诱蛾高峰视为羽化高峰,如在桃小食心虫成虫发生期预测时,有些将田间性诱剂诱蛾数量消长变化规律作为桃小食心虫雄成虫发生期的观测结果(见《北方园艺》1990年第5、6期“桃小性诱剂在枣树上测报与应用”一…  相似文献   

7.
基于神经网络的灯诱法预测马尾松毛虫发生量的研究   总被引:1,自引:0,他引:1  
【目的】为了探索灯诱马尾松毛虫Dendrolimus punctatus(Walker)成虫预测下一代幼虫发生量的办法,建立预测预报模型。【方法】2013-2017年连续5年采用灯诱法对马尾松毛虫每年的越冬代、第1代灯诱成虫数、雌虫数、雄虫数、雌性比等数据进行收集,实地调查第1代、第2代(越冬代)幼虫林间的发生量(虫口密度),采用Excel2016进行相关性分析,筛选出与下一代幼虫发生量(虫口密度)关系密切的灯诱成虫数、雌虫数、雌性比等关联因子,并应用神经网络Matlab2016a建立预测模型。【结果】所建立的灯诱成虫预测模型,拟合度0.92以上,预测精度0.90以上。【结论】采用灯诱马尾松毛虫成虫预测下一代幼虫发生量的神经网络Matlab模型,十分适用于短期精细化预报,方法简单、实用,值得在马尾松毛虫生产性防控中大力推广。  相似文献   

8.
【目的】比较健康和机械损伤的薄荷Mentha spicata对小菜蛾Plutella xylostella成虫嗅觉反应的影响,为筛选小菜蛾植物源驱避剂提供科学依据。【方法】利用触角电位(EAG)仪和Y型嗅觉仪分别测定小菜蛾雌、雄成虫对健康和机械损伤薄荷的触角电位和嗅觉行为反应,通过田间试验测定雌蛾产卵选择的忌避反应。【结果】健康薄荷和机械损伤薄荷均能引起小菜蛾雌、雄成虫触角电位反应,但损伤薄荷引起的触角电位反应值显著高于健康薄荷(P0.05);室内行为选择试验结果显示,损伤薄荷对小菜蛾雄性成虫有显著驱避作用(P0.05),驱避指数为53.33%,对雌性成虫有极显著驱避作用(P0.01),驱避指数达到80%;田间产卵选择试验发现,损伤薄荷对小菜蛾雌成虫产卵有极显著驱避作用(P0.01),产卵忌避指数达到71.32%。但是健康薄荷对小菜蛾雌、雄成虫的行为选择和雌性成虫的产卵选择均无显著驱避作用(P0.05)。【结论】机械损伤薄荷对小菜蛾成虫有较强的驱避效果,可以对其进行驱避活性挥发物质的筛选或作为驱避源来防治小菜蛾的危害。  相似文献   

9.
小菜蛾对合成植物挥发物的活性反应   总被引:2,自引:0,他引:2  
在室内检验了小菜蛾已交配和未交配雌、雄成虫对3种合成植物挥发物乙酸顺-3-己烯酯(Z-3-hexenyl acetate,ACTE)、异硫氰酸丙烯酯(allyl isothiocyanate,NCS)和顺-3-己烯醇(Z-3-hexen-1-ol,OH)的触角电位(EAG)反应。不同浓度(0.008 μg/μL、0.08 μg/μL、0.2 μg/μL、0.8 μg/μL、8 μg/μL、20 μg/μL和40 μg/μL)实验表明,随着刺激化合物浓度的提高,小菜蛾反应活性增强。但小菜蛾的性别及其交配状态可能影响其对3种化合物的反应强度:不同性别及交配状态的小菜蛾对ACTE的反应差异不大;未交配雌、雄虫对OH的反应强于已交配雌、雄虫;未交配雌虫对低浓度的NCS反应较强,NCS超过一定浓度时已交配雌虫的反应强于未交配雌虫,雄虫对NCS反应较小且与交配状态无关。以小菜蛾性信息素作为对照,在湖北长阳和越南河内试验了这3种植物挥发物诱芯(6 μL/诱芯)对小菜蛾的引诱作用。结果表明,在湖北,第1天时 ACTE、NCS对雄虫具有很强的引诱作用,引诱量显著大于性诱剂的引诱量,但随着诱芯放置时间延长,NCS引诱作用迅速下降,第2天时引诱作用已经很小;ACTE的引诱作用下降缓慢,第4天的诱蛾量才显著小于性信息素的诱蛾量,OH的引诱力较弱。在河内,ACTE、NCS、OH第1天对小菜蛾引诱作用很强,引诱量大于性信息素,但差异不显著;随着诱芯放置时间延长,3种挥发物对小菜蛾的引诱量均下降很快,第4天的引诱力就很弱。3种植物挥发物的混合物(体积比1∶1∶1)对小菜蛾的引诱作用与性信息素相似,且诱蛾活性持效期相对较长。无论在越南或湖北,植物挥发物或其混合物均很少引诱到雌虫。  相似文献   

10.
小菜蛾Plutella xylostella Linnaeus是一种世界性的十字花科蔬菜重要害虫,具有远距离迁飞的特性,明确小菜蛾在我国的种群发生动态及迁飞路径对其早期预警具有重要意义.本文调查了2009年5月我国南京、故城、安阳、大连、公主岭、沈阳6个地区小菜蛾成虫种群的发生动态,并首次利用HYSPLIT平台对不同地区小菜蛾种群的迁飞峰次进行了轨迹分析.结果表明,2009年5月我国6个地区的小菜蛾成虫种群存在显著地“突增”或“突减”现象,符合迁飞昆虫在迁飞期的种群动态的典型特征;5月14日南京地区起飞的小菜蛾种群可迁飞至大连,5月19日故城地区起飞的小菜蛾种群可迁飞至公主岭,5月20日安阳地区起飞的小菜蛾种群可迁飞至沈阳;首次明确了小菜蛾轨迹分析的设定参数,800-1200 m为小菜蛾适宜迁飞的飞行高度,飞行持续时间一般为2-3d.  相似文献   

11.
颗粒体病毒在小菜蛾种群中的垂直传播方式的初步观察   总被引:1,自引:0,他引:1  
吕利华  何余容 《昆虫知识》2004,41(6):559-562
在室内观察了小菜蛾颗粒体病毒PlutellaxylostellaGranulosisVirus(RxGV)在小菜蛾种群水平上可能的垂直传播途径。结果表明 :(1 )上代小菜蛾Plutellaxylostella(L .)幼虫期饲毒不能将小菜蛾颗粒体病垂直传递到下代 ;(2 )取食病毒的雌蛾产的卵所孵化的幼虫中 ,没病毒感染出现 ,说明病毒不能经小菜蛾成虫体内传播到下一代。 (3 )用小菜蛾颗粒体病毒处理卵卡时 ,其初孵幼虫有少量被病毒感染死亡 ,浓度高时感染率也较高 ,但感染率均在 1 0 %以下 ,说明小菜蛾颗粒体病毒能经卵表垂直传递给下代 ,但传递效率不高。但当用病毒处理叶片上的卵时 ,初孵幼虫死亡率高达 77 73 % ,而且孵化出的幼虫大多数染病且死于 3龄以前 ,因此田间病毒的施用时间可提早到卵高峰期。  相似文献   

12.
亚麻荠对小菜蛾幼虫取食和成虫行为反应的影响   总被引:5,自引:0,他引:5  
亚麻荠是一种很少有害虫危害的油料作物。用室内生测和Y 型嗅觉仪研究了亚麻荠对小菜蛾Plutella xylostella幼虫取食和成虫行 为反应的影响。以甘蓝作对照,用亚麻荠叶片喂养的小菜蛾初孵幼虫3天后校正死亡率为79 .2%,显示了较强的致死作用;喂养小菜蛾3龄幼虫至化蛹,其存活率、化蛹率、蛹重及成 虫寿命都显著降低,表明亚麻荠对小菜蛾幼虫的生长发育有不利影响。在幼虫的取食选择实 验中,有甘蓝叶供选择时,小菜蛾幼虫不取食亚麻荠;在无可选择的情况下,小菜蛾幼虫也 取 食亚麻荠叶片,但取食量很小,与取食甘蓝叶的量相比,差异极显著。行为反应测试表明, 小菜蛾成虫对甘蓝和亚麻荠植株的挥发物都有明显的趋性反应,与对照(净化空气)相比, 差异极显著,而在甘蓝和亚麻荠之间无选择性。说明小菜蛾成虫对亚麻荠植株的挥发物具有 较强的定向反应。  相似文献   

13.
四种十字花科蔬菜上小菜蛾自然种群连续世代生命表   总被引:13,自引:3,他引:10  
吕利华  何余容  庞雄飞 《生态学报》2003,23(12):2624-2630
利用作用因子生命表技术,组建芥菜、芥蓝、小白菜和菜心4种十字花科蔬菜上小菜蛾自然种群连续世代生命表,分析寄主植物和生物因子对小菜蛾种群数量的控制作用。结果表明:在4种十字花科蔬菜的一造菜上,小菜蛾均能完成两个世代。虽然小菜蛾在芥菜上的初始卵量最高,但害虫种群总增长倍数在芥蓝上最高,其次为小白菜,菜心和芥菜,分别为17.64、11.90、11.43和3.76。这说明尽管芥菜对小菜蛾成虫的产卵有一定的吸引作用,但不适合小菜蛾生长发育。芥蓝是最适宜小菜蛾种群增长的寄主。生物因子在小菜蛾自然种群控制中起着重要的作用,但是在不同种类十字花科蔬菜上,天敌类群对小菜蛾控制作用存在一定差异。除芥菜之外,寄生性天敌对芥蓝、菜心和小白菜上的小菜蛾种群控制作用最大,其次为“捕食及其它”,病原微生物的控制作用最小。“捕食及其它”对芥菜上小菜蛾种群的作用非常明显,如果排除此因子作用,小菜蛾种群两代后将增长126.03倍。该因子是导致芥菜小菜蛾自然种群增长趋势指数低的主要原因。因此在制定小菜蛾防治策略时,应考虑蔬菜的种类和布局,加大对芥蓝小菜蛾种群的防治力度;芥菜可作为一种诱杀植物种植,以吸引小菜蛾产卵,并集中防治。这些防治策略在小菜蛾综合治理中具有重要的实际意义。  相似文献   

14.
Studies were conducted to evaluate the toxicity of insecticides to adult Diadegma insulare (Cresson) and its host the diamondback moth, Plutella xylostella (L.). Leaf-dip and direct-dip bioassays for diamondback moth larvae and residual bioassays for adults of diamondback moth and D. insulare were used to assess mortalities. Larval mortalities at field rates were significantly higher with carbaryl, permethrin, spinosad, and tebufenozide when compared with Bacillus thuringiensis, or imidacloprid in the larval-dip bioassay 72 h after treatment. In the leaf-dip and residual bioassays, both permethrin and spinosad caused 100% mortalities to diamondback moth larvae and adults, respectively, 72 h after treatment. Of all the materials tested, only B. thuringiensis and tebufenozide were not toxic to D. insulare 24 h after treatment. Spinosad was not toxic to D. insulare 30 min after treatment. However, 100% mortality was observed 8 h after treatment.  相似文献   

15.
The prevalence of the diamondback moth and its larval parasitoids, was explored across Saskatchewan, Canada. Higher numbers of diamondback moths were recorded early in the season than later and in the Mixed Grassland compared to three other ecoregions. The highest proportion of parasitism was observed in the Boreal Transition ecoregion in early season.  相似文献   

16.
Canola (Brassica napus L.) cultivars Oscar and Westar, engineered with a Bacillus thuringiensis (Bt) cryIA(c) gene, were evaluated for resistance to lepidopterous pests, diamondback moth, Plutella xylostella L. (Plutellidae) and corn earworm, Helicoverpa zea (Boddie) (Noctuidae) in greenhouse and field conditions. In greenhouse preference assays conducted at vegetative and flowering plant stages, transgenic plants recorded very low levels of damage. A 100% diamondback moth mortality and 90% corn earworm mortality were obtained on transgenic plants in greenhouse antibiosis assays. The surviving corn earworm larvae on transgenic plants had reduced head capsule width and body weight. Mortality of diamondback moth and corn earworm were 100% and 95%, respectively, at different growth stages (seedling, vegetative, bolting, and flowering) on the transgenic plants in greenhouse tests. In field tests conducted during 1995–1997, plots were artificially infested with neonates of diamondback moth or corn earworm or left for natural infestation. Transgenic plants in all the treatments were highly resistant to diamondback moth and corn earworm larvae and had very low levels of defoliation. Plots infested with diamondback moth larvae had greater damage in both seasons as compared with corn earworm infested plots and plots under natural infestation. After exposure to defoliators, transgenic plants usually had higher final plant stand and produced more pods and seeds than non-transgenic plants. Diamondback moth injury caused the most pronounced difference in plant stand and pod and seed number between transgenic and non-transgenic plants. Our results suggest that transgenic canola could be used for effective management of diamondback moth and corn earworm on canola.  相似文献   

17.
Seasonal variation in plant quality may be intense enough to generate predictable patterns in insect herbivore populations. In order to explain seasonal oscillations in neotropical populations of the diamondback moth Plutella xylostella (L.), we tested the following: (1) if nutritional quality of cabbage (Brassica oleraceae var. capitata), a primary host plant of diamondback moth, adversely affects the performance of this insect in late spring and early summer, when populations decline and go extinct, and (2) if nutritional features of cabbage change with the seasons. We measured the performance of diamondback moth reared on leaves of cabbages grown during the four seasons of the year. Summer plants proved to be worse for the survival of the immature stages and subsequent adult fecundity, but there were no significant differences between the remaining seasons. Our results support the hypothesis that short-lived plants, grown in different seasons of the year in the tropics, have different nutritional and defensive attributes. We analyzed nutritional quality of cabbage leaves from the four seasons, but only total lipids were reduced in summer plants. Neotropical populations of diamondback moth collapse before plant quality decay in the summer. If the diamondback moth is well adapted to the seasonal deterioration of the habitat, including the reduction in the quality of host plants, it is expected that emigration happens before the mortality increases and natality decreases during the summer.  相似文献   

18.
The diamondback moth, Plutella xylostella (L.), is the most important pest of brassicaceous crops worldwide. Since temperature is the major abiotic factor influencing insect development and thermal requirements may vary among insect populations, it is important to know the effect of temperature on development and survival of a subtropical strain of P. xylostella. Development and survival of the diamondback moth was evaluated under seven constant temperatures ranging from 10°C to 35°C. Development was completed between 10°C and 32.5°C, but at 35°C all individuals died in the larval stage. Data were fitted to one linear and five nonlinear models. Considering as criteria the goodness of fit and the ability to estimate parameters of biological significance, the models Briere-1 and Briere-2 were the most adequate to describe the relationship between temperature and development of P. xylostella. The linear model demonstrated that P. xylostella required 312.5 degree-days above a lower threshold of 6.3°C to complete development. The degree-day model showed that the number of diamondback moth generations in the tropical region of Brazil is nearly twice the number in the subtropical region of the country. This result explains, at least in part, the higher population levels of this species in the tropical region of Brazil, and also demonstrates that P. xylostella is tolerant to a wide range of temperatures (6.1-32.5°C). Therefore, temperature cannot be considered a limiting factor for the occurrence of diamondback moth throughout the year in most regions of Brazil.  相似文献   

19.
The efficacy of lemon-scented tea tree oil (LSO), Leptospermum petersonii (FM. Bailey), was evaluated against the diamondback moth, Plutella xylostella (L.) under laboratory conditions. Feeding activity and development of larval stages were significantly reduced on broccoli leaves that had been dipped in LSO. Oviposition deterrence was also found when an adult stage was exposed to treated leaves. Fecundity dropped by >50% at concentrations >0.5%. The LC50 value for third instar larvae was estimated to be 2.93% 7 d after treatment. Experiments were also conducted under greenhouse conditions to assess the efficacy of LSO against the diamond-back moth. Our results suggest that LSO has modest potential for development as a botanical insecticide. The oil was also tested at concentrations from 0.5 to 6% for oviposition deterrence of an egg parasitoid of the diamondback moth, Trichogramma pretiosum (Riley). LSO deterred parasitization in choice tests but not in no-choice tests. LSO did not cause mortality of T. pretiosum during 24 h in a contact toxicity test. We conclude that LSO had no significant effects on the parasitoid, and therefore LSO is compatible with this biocontrol agent for integrated management of the diamondback moth.  相似文献   

20.
以安全性和简易性为前提的庭园害虫防治成为休闲生活不可或缺的技术。本文以花椒Zanthoxylum bungeamtm、八角茴香lUicium veturn、辣椒Capsicum frutescens等日用品为材料,研究了不同种类、浓度和作用时间对小菜蛾Plutella xylostella这一重要庭园害虫的拒食、触杀及产卵忌避效果。拒食试验表明,供试3种植物对小菜蛾有显著拒食作用,拒食率从大到小依次为八角茴香(0.94)、花椒(0.86)、辣椒(0.76);拒食率随处理时问长短变化的差异不显著,但随提取液浓度的升高而升高。触杀试验表明,供试3种植物对小菜蛾有显著触杀作用,24h内死亡率从大到小依次为花椒(45.6%)、八角茴香(35.6%)、辣椒(23.3%),均显著高于对照处理的1.1%;触杀死亡率随虫龄的增加而降低。产卵忌避试验表明,3种植物对小菜蛾成虫产卵忌避作用由大到小依次为辣椒、八角茴香、花椒。综合本研究所得结果来看,八角茴香对小菜蛾的控制效果最好。所选材料容易获得,对小菜蛾忌避作用明显,在庭园小菜蛾防治中具有潜在的应用价值。  相似文献   

设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号