普雄原鲵初步调查

普雄原鲵Pseudohynobius puxiongensis是依据1965年获得的单号标本描述订立的新种,目前仅见于我国大凉山极狭窄区域,是该高寒地区唯一的五趾小鲵科动物。调查小组根据相似分布区及访问线索,于2007—2014年间相继发现普雄原鲵个体(包括成体、幼体和卵袋)和3处栖息地。实地调查面积超过60 km2,其中潜在分布区约400 000 m2,发现普雄原鲵实际生存面积仅3425 m2。3处栖息地海拔2770~3000 m,地处彝族聚集区和牧区草场,主要生境是高山草甸和灌丛、高原山涧、小浸沟及其水坑、小水潭。栖息地分布有茂密的箭竹林、杜鹃丛和草丛等,较好地提供了荫蔽的环境。本次调查发现普雄原鲵成体20尾,幼体157尾,卵袋16对;3处栖息地的种群数量和种群密度相差较大。各栖息地受到不同程度的人为干扰和牲畜干扰。较之其他同类动物,普雄原鲵野外种群数量极其稀少。     

我国三种两栖动物在广西的新分布

在广西发现了3种两栖动物,它们分别是锄足蟾科的高山掌突蟾(Leptolalax alpinus)、景东角蟾(Megophrys fingdongenis)、大角蟾(M．major)。     

中国五种高山锄足蟾的核型、Ag-NORs和C-带的研究

作者用核型、Ag-NORs和C-带,对分布于川、滇两省的二属(齿突蟾、齿蟾)五种(胸腺齿突蟾、圆疣齿突蟾、凉北齿蟾、秉志齿蟾、疣刺齿蟾)锄足蟾作了属间和种间关系的比较分析,并讨论了它们的核型演化机制。结果表明:(1)齿突蟾和齿蟾两属间在核型和带型上都有明显的差异,演化途径主要的可能是含有重复DNA染色体片段的相互易位或臂间倒位;(2)属内不同种之间带型无显著差异,但某些对应染色体对间,其相对长度和臂比值差异明显,十分可能是常染色质片段的易位和臂间倒位所致;(3)凉北齿蟾有染色体数目变异多态现象;(4)五种锄足蟾均未发现异形性染色体。     

云南三种齿蟾的细胞遗传学研究(锄足蟾科Pelobatidae,无尾目Anura)

本文报道了分布在云南的3种齿蟾(乡城齿蟾、景东齿蟾和棘疣齿蟾)的核型、Ag-NORs和C-带,结果表明2n=26(6+7).NF=52,一对次缢痕和Ag-NORs位于6 q,该区域同时被C-带正染,其余C-带位于各对染色体的着丝点区域。这些特征表现出3种齿蟾的核型同源性和原始性。齿蟾属内种间各对应染色体对在相对长度和臂比值的差异显著性测定表明其核型演化途径可能是臂间倒位和相互易位。3种齿蟾具有染色体数目和Ag-NORs的变异和异形现象,其中乡城齿蟾的一个多余染色体呈现C-带正染,因此可能是B染色体,是锄足蟾科中首次报道。三种齿蟾均未发现与性别相关的异形染色体。     

瑶山髭蟾的核型

髭蟾是我国特有的无尾两栖类,主要分布在川、黔、滇、桂、浙、闽等省的山区。自刘承钊于1945年建立髭蟾属(Vibrissaphora Liu)以来,已先后发表有五种,即峨嵋髭蟾(Vibrissaphora boringii Liu),崇安髭蟾(V.liui Pope),雷山髭蟾(V.leishanensis Liu et Hu),瑶山髭蟾(V.yaoshanensis Liu et Hu)和哀牢髭蟾(V.ailaomica Yang,Chen et Ma)。赵尔宓、吴贯夫(1983,1987)报道了全部髭蟾种的核型,并提出了瑶山髭     

亚洲高海拔锄足蟾的属间亲缘关系、分化及其与青藏高原形成的关系

本文在研究高海拔锄足蟾的属间分类及分布规律的基础上,对所属的齿蟾属Oreolalax、齿突蟾属Scutiger的齿突蟾亚属Scutiger,(Scutiger)和猫眼蟾亚属Scutiger(Aelurophryne)的成体外形和骨骼、蝌蚪的形态以及核型等24个性状进行综合分析,同时探讨了属间亲缘关系。结果表明:齿蟾属较原始,齿突蟾属较特化,它们可能起源于共同的祖先——原始齿蟾。在齿突蟾属中猫眼蟾亚属比齿突蟾亚属更为特化,后者与齿蟾属的亲缘关系相对较近。它们的分化与青藏高原的抬开有密切关系。     

齿突蟾属某些种的多态现象

本文报道某些锄足蟾科Pelobatidae动物的多态现象。讨论了同域或不同域的四种齿突蟾Scuti-ger,即刺胸齿突蟾S. mammatus(Guenther)、胸腺齿突蟾S. glandulatus(Liu)、木里齿突蟾S. mulicnsis Fei et Ye和皱纹齿突蟾S. ruginosus Zhao et jiang的皮肤瘰疣、第二性征等性状;用薄层等电聚焦电泳方法分析了同域三种齿突蟾的晶体蛋白。结果表明:前三种是有效种;后一种,即皱纹齿突蟾分别是前三种的多态性变体;这些变体以相同性状(即皮肤多瘭疣)同时表现在三个种中的现象,属于平行多态现象。     

五种齿突蟾在横断山南潜在地理分布预测

由于栖息地质量下降,近年来齿突蟾属物种种群数量急剧减少,明确齿突蟾属物种空间分布,是监测、管理、保护齿突蟾属物种的基础。横断山区可能是齿突蟾属的起源中心和分化中心,但齿突蟾属在横断山区的地理分布格局尚不明确。利用优化后Maxent模型,首次预测西藏齿突蟾Scutiger boulengeri、刺胸齿突蟾Scutiger mammatus、胸腺齿突蟾Scutiger glandulatus、圆疣齿突蟾Scutiger tuberculatus、贡山齿突蟾Scutiger gongshanensis 5种高海拔齿突蟾属物种在横断山南生物多样性保护优先区域的潜在地理分布,并分析其与环境因子的关系。结果显示,5种齿突蟾属物种在横断山南的潜在地理分布格局存在差异,西藏齿突蟾主要分布在横断山南的北部,圆疣齿突蟾主要分布在横断山南东北部的四川省境内,贡山齿突蟾主要分布在横断山南的西南部,刺胸齿突蟾和胸腺齿突蟾的潜在分布格局较为相似,在横断山南的中部、西北部地区都有较多分布,但胸腺齿突蟾潜在分布区更为碎片化。另外,横断山南北部地区的齿突蟾属丰富度明显高于南部地区。环境变量贡献率和刀切法结果显示温度因子和降水因子是决定横断山南齿突蟾属潜在分布的主要因素,最冷季降水量对西藏齿突蟾、贡山齿突蟾、圆疣齿突蟾潜在分布有重要影响,但它们对最冷季降水量的偏好存在差异。此外,研究也显示,通过评估潜在的Maxent参数组合,选择最佳的Maxent模型是有效且必要的。     

亚洲高海拔锄足蟾的地理分布特点,起源与演化,分化中心的探讨

本文在探讨亚洲高海拔锄足蟾的属间分类的基础上,进一步对其齿蟾属Oreolalax和齿突蟾属Scutiger所隶的齿突蟾亚属Scutiger(Scutiger)、猫眼蟾亚属Scutiger(Aelurophryne),共计29种的地理分布及其特点作了概括性的综述;并探讨了他们的起源和演化途径;横断山系地区可能是它们的主要分化中心和物种形成中心。     

瓦屋山国家森林公园锄足蟾科6种的繁殖鸣声特性

在地处四川省洪雅县的瓦屋山国家森林公园录取了锄足蟾科 6种的繁殖期求偶鸣叫声。它们分隶 4属 ,即角蟾属 (Megophrys)、齿蟾属 (Oreolalax)、齿突蟾属 (Scutiger)和掌突蟾属 (Leptolalax)。在IBMPC上用“SIGNAL”软件 (EngineeringDesign ,USA)对获取的鸣声资料进行分析 ,分析的频率范围设置为 0～ 10kHz。声学分析结果表明 :峨山掌突蟾 (L oshanensi) ,小角蟾 (M minorr) ,角蟾 1种 (M sp) ,金顶齿突蟾[S (S )chintingensis],峨眉齿蟾 (O omeimontis)和无蹼齿蟾 (O schmidti)的主能峰频率平均值分别是45 2 1 9、 34 5 6 4、 2 2 93 8、 10 76 5、 10 71 0和 1849 4Hz ,每声持续时间的平均值分别是 46 2、 90 8、 99 6、72 2、 78 8和 110 3ms ,声距的平均值分别是 140 4、 2 5 3 0、 6 81 4、 15 17 7、 46 1 3和 6 19 5ms。单因子方差分析结果表明主能峰频率、每声持续时间和各声距在 6个种间差异极显著 (P <0 0 1)。LSD法多重比较的结果指出金顶齿突蟾和峨眉齿蟾间的主能峰频率无显著差异 (P =0 917>0 0 5 ) ;在每声持续时间上 ,只有峨山掌突蟾与小角蟾、角蟾 1种、峨眉齿蟾、无蹼齿蟾间差异极显著 (P <0 0 1) ;在声距上 ,峨山掌突蟾与小角蟾间无显著差异 ,角蟾 1种与无蹼齿蟾之间、峨     

Curcuminoids as inhibitors of thioredoxin reductase: A receptor based pharmacophore study with distance mapping of the active site

Curcumin is the yellow pigment of turmeric that interacts irreversibly forming an adduct with thioredoxin reductase (TrxR), an enzyme responsible for redox control of cell and defence against oxidative stress. Docking at both the active sites of TrxR was performed to compare the potency of three naturally occurring curcuminoids, namely curcumin, demethoxy curcumin and bis-demethoxy curcumin. Results show that active sites of TrxR occur at the junction of E and F chains. Volume and area of both cavities is predicted. It has been concluded by distance mapping of the most active conformations that Se atom of catalytic residue SeCYS498, is at a distance of 3.56 from C13 of demethoxy curcumin at the E chain active site, whereas C13 carbon atom forms adduct with Se atom of SeCys 498. We report that at least one methoxy group in curcuminoids is necessary for interation with catalytic residues of thioredoxin. Pharmacophore of both active sites of the TrxR receptor for curcumin and demethoxy curcumin molecules has been drawn and proposed for design and synthesis of most probable potent antiproliferative synthetic drugs.     

A Unique Protease Cleavage Site Predicted in the Spike Protein of the Novel Pneumonia Coronavirus (2019-nCoV) Potentially Related to Viral Transmissibility

正Dear Editor,In December 2019, a novel human coronavirus caused an epidemic of severe pneumonia(Coronavirus Disease 2019,COVID-19) in Wuhan, Hubei, China(Wu et al. 2020; Zhu et al. 2020). So far, this virus has spread to all areas of China and even to other countries. The epidemic has caused 67,102 confirmed infections with 1526 fatal cases     

Comparative morphology of the carpel in the Liliaceae: Hewardieae, Petrosavieae, and Tricyrteae

The young pistils in the melanthioid tribes, Hewardieae, Petrosavieae and Tricyrteae, are uniformly tricarpellate and syncarpous. They lack raphide idioblasts. All are multiovulate, with bitegmic ovules. The Petrosavieae are marked by the presence of septal glands and incomplete syncarpy. Tepals and stamens adhere to the ovary in the Hewardieae and the Petrosavieae but not in the Tricyrteae. Two vascular bundles occur in the stamens of the Hewartlieae and Tricyrtis latifolia. Ventral bundles in the upper part of the ovary of the Hewardieae are continuous with compound septal bundles and placental bundles in the lower part. Putative ventral bundles occur in the alternate position in the Tricyrteae and putative placental bundles in the opposite. position in the Petrosavieae. The dichtomously branched stigma in each carpel of the Tricyrteae is supplied by a bifurcated dorsal bundle.     

鸡传染性法氏囊病病毒研究进展

传染性法氏囊病(Infection bursal disease, IBD)是由鸡传染性法氏囊病毒(Infectious bursal disease virus, IBDV)引起的鸡和火鸡的一种高度接触性传染病,给世界各国的禽养殖业带来了巨大损失.自IBDV发现至今新的变异株不断出现,分子结构的改变导致病毒致病力的改变及宿主对疫苗应答的改变,使得传统的疫苗已不能控制其流行,因此各国学者对其基因组结构和功能进行了广泛深入的研究,并积极研制新型有效的疫苗以达到防治的目的.     

Development of a Novel Reverse Transcription Loop-Mediated Isothermal Amplification Method for Rapid Detection of SARS-CoV-2

In conclusion, the novel visual RT-LAMP assay is a simple, rapid, and sensitive approach for detection of SARS-CoV-2, and it is ready for application in primary care and community hospitals or health care centers, and even patients' own houses in response to the current SARS-CoV-2 epidemic because the assay does not require sophisticated equipment and skilled personnel. Furthermore, it is also ready to be used in fields for screening samples from wild animals and environments to facilitate the identification of potential intermediate hosts that mediate the cross-species transmission of SARS-CoV-2 from bats to humans.     

Perinatal Vertical Transmission of Chikungunya Virus in Ruili,a Town on the Border between China and Myanmar

正Dear Editor,Chikungunya virus (CHIKV), an arbovirus in the family of Togaviridae, genus Alphavirus, is transmitted by the A.aegyptii or A. albopictus mosquito, and causes disease in humans characterized by fever, rash, and arthralgia (Silva and Dermody 2017; Suhrbier 2019). It was first reported in 1953 in Tanzania, and caused only a few outbreaks and sporadic cases in Africa and Asia in last century. However, in the epidemic in 2004, CHIKV acquired mutations that conferred enhanced transmission by the A. albopictus mosquito(Schuffenecker et al. 2006). Since then, it has successively caused outbreaks in Africa, the Indian Ocean, South East Asia, the South America, and Europe (Zeller et al. 2016).