共查询到17条相似文献,搜索用时 62 毫秒
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目的:比较富硒北虫草和普通北虫草的总硒含量、无机硒含量和有机硒含量。方法:采用紫外分光光度法测定富硒北虫草和普通北虫草的总硒含量和无机硒含量,然后通过计算得出有机硒含量。结果:富硒北虫草中总硒含量高于普通北虫草70倍左右,而有机硒含量则大约是普通北虫草的100倍,且富硒北虫草中有机硒含量为总硒含量的97%左右。结论:鞍山华宇生物科技有限公司开发的富硒北虫草中有机硒的含量明显高于普通北虫草,具有良好的使用前景。 相似文献
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为探究饲料中高剂量的有机硒和无机硒对异育银鲫(Carassius auratus gibelio var. CAS V)的生长性能、硒蓄积和血浆生化指标等方面的影响,以硒代蛋氨酸(有机硒)和亚硒酸钠(无机硒)作为不同类型的硒源,初始体重为(62.95±0.23) g异育银鲫为研究对象,进行了为期90d的养殖实验。实验结果表明,饲料中添加0、10和20 mg/kg的有机硒和无机硒对异育银鲫的存活和饲料干物质的表观消化率无显著影响;有机硒处理组硒的表观消化率随饲料有机硒的添加量增加而显著升高(P<0.05);饲料中添加无机硒对硒消化率无显影响(P>0.05)。在饲料中添加有机硒可以提高异育银鲫的生长(P<0.05),在20 mg/kg处理组中达到最高(P<0.05);而饲料中添加10 mg/kg无机硒处理组未显著改变异育银鲫的特定生长率(P>0.05),但高浓度的无机硒则显著降低了其特定生长率(P<0.05)。饲料中添加有机硒显著降低了异育银鲫的肝体比,添加10 mg/kg无机硒显著降低异育银鲫的肝体比(P<0.05);饲料中添加有机硒和无机硒对异... 相似文献
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沼泽红假单胞菌富硒发酵条件的研究 总被引:1,自引:0,他引:1
通过对沼泽红假单胞菌富硒发酵条件的研究,确定最优的富硒培养方式。采用单一因素变量法,利用微波消解与紫外分光光度法测定沼泽红假单胞菌在不同发酵条件下的生物量变化与富硒效果,确定其最佳培养方式。结果表明,最佳富硒培养条件为:培养温度30℃,环境硒浓度100μg/mL,培养基初始pH为7,硒添加方式应为梯度分次添加,添加时间应为培养周期的第3天、第4天。利用得到的富硒菌种,在最优条件下培养7 d后,测得环境硒浓度下降为10.9μg/mL,硒的转化率为89.1%,菌体富硒量可达到73.54 mg/g(干菌种),其中有机硒含量为97.1%。利用沼泽红假单胞菌生产有机硒具有可行性,富硒菌体可以作为动物饲料添加剂,也可以为人类提供富含有机硒的食品。在以后的实验中,将进一步进行验证和工业化应用。 相似文献
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通过筛选‘红富士’苹果适宜的叶面施硒浓度,研究了营养元素硒在苹果叶片中的赋存形式、分布及含硒大分子的提取分离技术。结果表明,(1)‘红富士’苹果最佳叶面施硒浓度为100mg·L-1,此处理的叶片总硒含量为2.7283mg·L-1(FW),其中有机硒含量为2.3384mg·L-1(FW),有机化程度达85.71%。(2)无论在对照组还是处理组,叶片中的有机硒均主要以蛋白质结合态存在,平均占有机硒的77.09%。(3)蛋白质、多糖和核酸3种有机物在100mg·L-1最优处理组的叶片中结合硒的量分别占有机硒含量的77.42%、18.46%和0.54%。(4)在100mg·L-1处理组的蛋白质组分中,又以盐溶性蛋白质结合硒的量最多,占蛋白质结合态硒含量的46.27%。 相似文献
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《生物技术进展》2016,(6)
为研究小麦从籽粒加工成馒头过程中硒的变化规律,选用小麦品种泰农18为材料,通过测定喷施硒肥的泰农18籽粒、面粉、麸皮和馒头中硒的不同结合形态,分析硒在馒头加工过程中的变化规律。结果表明,小麦籽粒硒在面粉及其馒头的加工过程中,主要以有机态形式存在,其中,籽粒、面粉、麸皮和馒头中有机态硒分别占总硒的88.4%、83.1%、87.4%和79.2%。在小麦籽粒、面粉、麸皮和馒头的蛋白结合态硒中,麦谷蛋白结合态硒含量最高,分别占有机硒的16.32%、9.68%、8.94%和8.39%。在小麦从籽粒到馒头的加工过程中,总硒和有机硒有一定程度的损失。研究结果为利用富硒小麦加工产品馒头对人体进行科学补硒具有重要意义。 相似文献
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采用微波水解、HPLC-HG-AFS法测定了硒蛋白粉、硒蛋白片、肽粉、富硒原料等19种硒产品中的总硒、硒代氨基酸和亚硒酸根离子[Se(IV)],分析了硒代氨基酸、Se(IV)和其他形态硒占总硒的百分比及不同形态硒代氨基酸的组成比例。以此为依据,将硒产品分为硒蛋白型、单一硒代氨基酸型、其它形态硒型及有机无机硒混合型。根据DBS42/002-2014规定建立了富有机硒产品评分模式,其中18种为富有机硒产品;根据适硒地区母乳中硒代氨基酸的组成比例提出了硒代氨基酸的化学评分模式,评分结果显示13种以蛋白态硒为主的硒产品中硒代氨基酸的组成比例均与母乳相差甚远,不利于人体平衡吸收利用,其中10种SeMet含量远远超过人体所需,SeCys_2为限制硒代氨基酸。该评分模式的建立对硒产品的开发具有指导意义。 相似文献
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不同消化方法对测定生物样品中硒含量的比较研究 总被引:6,自引:0,他引:6
瞿进文 《天然产物研究与开发》1999,11(2):44-47
本文比较了干法消化、普通湿法消化、高压消化、微波消化对测定富含有机硒的生物样品中硒含量的影响.结果显示,高压及微波消化等密闭消化体系具有显著的优点,适合生物样品中富含有机硒的硒含量的测定. 相似文献
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Shrutika L. Wadgaonkar Yarlagadda V. Nancharaiah Giovanni Esposito Piet N. L. Lens 《Critical reviews in biotechnology》2018,38(6):941-956
Selenium concentrations in the soil environment are directly linked to its transfer in the food chain, eventually causing either deficiency or toxicity associated with several physiological dysfunctions in animals and humans. Selenium bioavailability depends on its speciation in the soil environment, which is mainly influenced by the prevailing pH, redox potential, and organic matter content of the soil. The selenium cycle in the environment is primarily mediated through chemical and biological selenium transformations. Interactions of selenium with microorganisms and plants in the soil environment have been studied in order to understand the underlying interplay of selenium conversions and to develop environmental technologies for efficient bioremediation of seleniferous soils. In situ approaches such as phytoremediation, soil amendment with organic matter and biovolatilization are promising for remediation of seleniferous soils. Ex situ remediation of contaminated soils by soil washing with benign leaching agents is widely considered for removing heavy metal pollutants. However, it has not been applied until now for remediation of seleniferous soils. Washing of seleniferous soils with benign leaching agents and further treatment of Se-bearing leachates in bioreactors through microbial reduction will be advantageous as it is aimed at removal as well as recovery of selenium for potential re-use for agricultural and industrial applications. This review summarizes the impact of selenium deficiency and toxicity on ecosystems in selenium deficient and seleniferous regions across the globe, and recent research in the field of bioremediation of seleniferous soils. 相似文献
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植物硒及其含硒蛋白的研究 总被引:5,自引:0,他引:5
硒是植物的有益元素,植物对硒的吸收与外源硒的有效性、硒的形态、植物的种类等有关;硒在植物中主要以有机硒形态存在,HPLC-ICP-MS联用已成为植物体内硒形态鉴定的最常用手段;含硒蛋白是植物体内最主要的有机大分子硒,具有抗肿瘤、抗氧化等多种生物活性。在环境安全和人类健康等方面,富硒植物具有很好的应用价值,所以利用分子生物学手段分析富硒植物的富硒机制,可以为富硒基因的筛选和利用提供理论依据。 相似文献
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. Suhajda J. Hegczki B. Janzs I. Pais G. Vereczkey 《Journal of trace elements in medicine and biology》2000,14(1)
Selenium (Se) is an essential micronutrient for human and animal organisms. Organic selenium complexes and selenium-containing amino acids are considered the most bioavailable.Under appropriate conditions yeasts are capable of accumulating large amounts of trace elements, such as selenium, and incorporating them into organic compounds. It has been found that introduction of water-soluble selenium salt as a component of the culture medium for yeasts produced by conventional batch processing results in a substantial amount of selenium being absorbed by the yeast.Using a culture medium supplemented with 30 μg/mL sodium-selenite added during the exponential growth phase results in selenium-accumulation in the range of 1200–1400 μg/g dried baker's yeast (Saccharomyces cerevisiae) measured by ICP-AES method. In our previous studies it was shown that higher amounts of sodium-selenite in the culture medium have a strong inhibitory effect on the growth of this yeast. As a consequence of variations in cultivation conditions we obtained selenium yeast with different inorganic selenium content. The most important parameters influencing incorporated forms of selenium are pH value and dissolved oxygen level in the culture medium, and depending on these the selenium consumption rate of the yeast. A 0.40–0.50 mg/g h-1 specific selenium consumption rate was found to be appropriate to obtain selenium-enriched bakers' yeast of a high quality. Under suitable conditions the undesirable inorganic selenium content of the yeast could be suppressed to as low as 5–6% at the expense, however, of approximately a 20% decrease in the final biomass. 相似文献
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植物硒同化的研究进展及其耐硒突变体的筛选 总被引:9,自引:0,他引:9
陈大清 《氨基酸和生物资源》2004,26(2):65-71
简要叙述了高等植物对硒元素吸收、同化的过程和特点。对近年来植物硒蛋白存在状态、硒相关突变体的筛选和应用硒代谢关键酶基因的转基因植物研究等方面的最新进展作了扼要综述。在此基础上对植物硒营养研究的发展提出了几点设想。 相似文献