盐碱池塘浮游植物初级生产力的研究

根据黑白瓶测氧法测定 ,山东省高青县赵店渔场盐碱池塘鱼类生长期内 (4— 9月 )浮游植物初级生产力为9 42± 4 2 1gO2 (m2 ·d) ,日P/B系数平均 0 2 4± 0 18,浮游植物对太阳有效辐射的利用率平均 1 5 3%。无鱼对照池浮游植物初级生产力显著低于养鱼池。回归分析表明 ,浮游植物现存量、透明度、水温、盐度是决定盐碱池塘浮游植物初级生产力的主要生态因子。营养盐中磷比氮的限制作用大。文中探讨了养鱼池初级生产力在能量转化中的生态学效率。     

池塘浮游植物的初级生产力及其与若干生态因子间的关系

本文应用黑白瓶法测定了浮游植物的初级生产力,分析了初级生产力与主要生态因子间的关系。晴天,水柱的最高生产层常出现在水下0.5倍透明度左右的水层,此处光强约为20000Lx,补偿深度常随天气变化。#br#浮游植物的生产量与生物量和镁之间常表现出正相关。生长季节内单位水面下浮游植物的P/B系数为177.7,日平均1.4。浮游植物对总辐射能的利用率为0.31%。氮是三江平原池塘浮游植物初级生产力的主要限制因子。     

刺参养殖池塘初级生产力及其粒级结构周年变化

研究了刺参(Apostichopus japonicus Selenka)养殖池塘浮游植物初级生产力及粒级结构的周年变化规律,旨在明确刺参养殖池塘的基础生态学特征,为刺参养殖生产和管理提供科学支持。结果表明:刺参养殖池塘初级生产力年平均值为(5.16±3.04)gO2m-2d-1,全年呈现明显的季节变化,初级生产量分别在初春、夏季和初冬形成高峰。初级生产力群落净产量占毛产量的50.2%。P/R值与日P/B系数的年平均值分别为2.20±1.25和0.39±0.35。按初级生产力水平和P/R值划分的水体营养类型,调查刺参养殖池塘属富营养型水体;初级生产量随深度的增加而递减,最高生产层约在透明度的0.5倍处,且0.5倍透明度(约50 cm)以上水层初级生产量占水柱总产量的56.3%;不同粒级浮游植物生产量占总生产量的百分比具有明显的季节变化。除夏季外,以小型浮游植物(micro-,20—200μm)对初级生产力的贡献最大(43.5%),夏季为微型浮游植物(nano-,2—20μm)对初级生产力贡献最大(35.3%)。以年平均值计算,不同粒级浮游植物初级生产量占总生产量百分比的大小顺序为:小型(40.1%)微型(28.2%)中大型(16.1%)超微型(15.7%)。回归分析表明:试验池塘初级生产力水平与水温、营养盐中的氨氮和亚硝酸氮均呈显著的相关关系(P0.05)。结果提示,刺参养殖池塘初级生产力的季节变化显著,垂直分布并不均匀,小型浮游植物是其生态系统中的主要生产者。     

太湖湖岸带浮游植物初级生产力特征及影响因素

利用高频溶解氧监测,估算了太湖梅梁湾湖岸带浮游植物初级生产力的高频变化特征。结合同步气象监测及浮游植物、浮游动物和营养盐的周年逐周观测数据,分析了气象和环境条件对富营养化湖泊浮游植物初级生产力的影响。结果发现,高频溶解氧监测估算的初级生产力变化与浮游藻类生物量的变化一致,能够反映出浮游植物生产力的昼夜变化、季节变化等规律。统计分析表明,气温对太湖这一富营养化湖泊初级生产力影响很大;氮的供给与浮游动物的选择性牧食也是影响浮游植物初级生产力的重要因素。湖岸带的水华堆积过程对初级生产力影响巨大,气象、水文过程又加剧了蓝藻水华初级生产力的变化幅度,反映出富营养化湖泊初级生产力可能存在极大的时空不均一性。研究表明,溶解氧高频监测法估算初级生产力能够捕捉到湖泊初级生产力的快速变化过程,可以用于富营养化湖泊初级生产力监测、蓝藻水华灾害预警中。     

三峡水库浮游植物初级生产力的季节变化与空间分布

研究在三峡库区选取秭归、巫山、云阳、忠县、木洞5 个断面, 自2012 年8 月至2013 年4 月采用黑白瓶法进行为期一周年季节性的原位初级生产力测定, 探讨初级生产力的季节变化、水平分布、垂直分布等动态特征。结果表明: 初级生产力平均值(以溶氧计)的四季变化规律为: 夏季5.613 mgO2/(Ld)春季3.630 mgO2/(Ld)冬季0.906 mgO2/(Ld)秋季0.552 mgO2/(Ld), 与浮游植物叶绿素a 含量的季节变化一致;在初级生产力的空间分布上, 5 个断面中库首最高(秭归)、库中次之(巫山、云阳、忠县)、库尾最低(木洞);且全年所有断面干流初级生产力均显著低于支流初级生产力;在垂直分布上, 初级生产力的最大值主要出现在水下0-1 m 间, 在水下1-5 m 随着水深增加呈现递减趋势, 光能可得性可能是限制浮游植物初级生产力的重要因子。相关性分析表明, 毛初级生产力与叶绿素a、溶解氧、pH 呈显著正相关。       

罗非鱼对盐碱池塘围隔浮生物群落的影响

本文报道了单养尼罗罗非鱼（Ｏｒｅｃｏｈｒｏｍｉｓ ｎｉｌｏｔｉｃｕｓ）对施肥处理下盐碱池塘围隔生态系统浮游生物群落的影响。结果表明,罗非鱼的放养使浮游植物丰度、叶绿素ａ含量和初级生产力增大,浮游植物小型化,生物量以小型硅藻和绿藻占优势,裸藻占有相当比重;浮游动物生物量也增大,桡足类占优势,枝角类小型化,原生动物密度增大。施肥特别是施有机肥能显著提高浮游植物生物量,使透明度降低,但施无机肥对初级生产     

罗非鱼对盐碱池塘围隔浮游生物群落的影响

本文报道了单养尼罗罗非鱼 (Orecohromisniloticus)对施肥处理下盐碱池塘围隔生态系统浮游生物群落的影响。结果表明 ,罗非鱼的放养使浮游植物丰度、叶绿素a含量和初级生产力增大 ,浮游植物小型化 ,生物量以小型硅藻和绿藻占优势 ,裸藻占有相当比重 ;浮游动物生物量也增大 ,桡足类占优势 ,枝角类小型化 ,原生动物密度增大。施肥特别是施有机肥能显著地提高浮游植物生物量 ,使透明度降低 ,但施无机肥对初级生产力和浮游动物生物量影响不大。施有机肥围隔浮游植物和浮游动物密度、浮游动物生物量和浮游生物多样性指数高于其他有鱼围隔 ,罗非鱼的生长最好。文后讨论了罗非鱼滤食和施肥对浮游生物群落结构的影响 ,并与鲢鱼的实验结果 (赵文 ,1999)进行了比较     

主养鲢鳙非鲫高产鱼塘的初级生产力与能量转化效率的研究

分析测定了主养鲢鳙非鲫高产池塘浮游植物初级生产力的垂直、周日、周年变化及其与主要生态因素、鱼产量水平的关系。探讨了初级生产力在能量转化中的生态学效率:毛初级生产力对太阳辐射能的利用率为0.84％—1.64％;鲢鳙非鲫净产量对浮游植物净产量的直接利用率为8.46％—15.56％;太阳能转化为成产量的生态学效率为0.058％—0.156％。     

盐碱池塘水生大型植物的研究

于１９９８年４月至１９９８年９月对高青盐碱池塘的水生大型植物种类组成,生产力及形影响因子进行了野外调查和试验研究,结果表明高青盐碱池塘共有水生植物１２种。常见的优势种有沉水植物：菹草、角果藻、金鱼藻、;挺水植物、芦苇、荆三棱、莲和香蒲漂浮植物;浮萍,以及大型藻类布氏轮藻。种类少而优势种突出是盐碱池塘大型植物的显特点,有水草池塘与无或水少草池塘相比,透明度大、ＰＨ值高、浮游植物生物量和叶绿素a含量低、营养盐含量也低。在表光照度７２００lx、水温２８－３０℃的条件下,菹草、角果藻和轮藻的毛产量分别为１．７０、１．５６和１．５０mgO2.L^-1.h^-1.g^-1。除光照、水温、ＰＨ等因素外,含盐量和碱度对大型植物的初级生产力具有重要影响,菹草和角果藻的生产力最高时的含直均为３g.L^-1,前适宜的碱度为６．６９mmol.L^-1,而后则为３．８２mmol.L^-1。菹草、角果藻、金鱼藻和浮萍对ＮＨ４－Ｎ的吸收速率相近,而对ＰＯ４－Ｐ的吸收速率则角果藻＞菹草＞浮萍＞金鱼藻。后对盐水水体大型植物特点、菹草生产力及其影响因素,如何防止养鱼池大型植物危害及大型植物与浮游植物的关系进行了讨论。     

鲢放养和施肥对盐碱池塘围隔生态系统浮游生物群落的影响

报道了单养链（Hypophthalmichthys molitrix)和施肥对盐碱池塘围隔生态系统浮游生物群落的影响,链的放养使浮游植物丰度,叶绿素a 含量和和初组生产力增大,浮游植物小型化,生物量以小型硅藻和绿藻占优势,裸藻和金藻占有相当比重;浮游动物生物量减少,特别是枝角类的生物量无鱼围隔大于有鱼围隔,且多是较大型的种类,施肥特别是施无机肥能显著地提高浮游植物丰度和初级生产力,浮游动物生物量也增大,施有机肥围隔浮游植物和浮游动物生物量虽高于有鱼对照围隔,但其浮游植物初级生产力,叶绿素a含量,浮游生物多样性指数,P／R系数均较低,链鱼的生长最差,文中讨论了滤食性鱼类和施肥对浮游生物的影响。     

盐碱池塘水生大型植物的研究

于1998 年4 月至1998 年9 月对高青盐碱池塘的水生大型植物种类组成、生产力及其影响因子进行了野外调查和试验研究。结果表明高青盐碱池塘共有水生植物12 种。常见的优势种有沉水植物:菹草、角果藻、金鱼藻;挺水植物:芦苇、荆三棱、莲和香蒲;漂浮植物:浮萍, 以及大型藻类布氏轮藻。种类少而优势种突出是盐碱池塘大型植物的显著特点。有水草池塘与无或少水草池塘相比, 透明度大、pH 值高、浮游植物生物量和叶绿素a 含量低、营养盐含量也低。在表层光照度7200lx、水温28~30℃的条件下, 菹草、角果藻和轮藻的毛产量分别为1.70、1.56 和1.50mgO₂·L^-1·h^-1·g^-1。除光照、水温、pH 等因素外, 含盐量和碱度对大型植物的初级生产力具有重要影响, 菹草和角果藻的生产力最高时的含盐量均为3g·L^-1, 前者适宜的碱度为6.69 mmol·L^-1, 而后者则为3.82 mmol·L^-1。菹草、角果藻、金鱼藻和浮萍对NH₄-N 的吸收速率相近, 而对PO₄-P 的吸收速率则角果藻 > 菹草 > 浮萍 > 金鱼藻。文后对盐水水体大型植物特点、菹草生产力及其影响因素、如何防止养鱼池大型植物危害及大型植物与浮游植物的关系进行了讨论。     

Do light/dark cycles of medium frequency enhance phytoplankton productivity?

It has been suggested that turbulence with the resultant light/dark cycle and light gradient through which phytoplankton move, enhances their productivity. The stationary bottle incubation technique for estimating rates of primary productivity has mainly been criticized because of bottle effects, the elimination of natural turbulence and the presence of photo-inhibition. In a series of experiments where productivity was measured over static profiles and compared to the productivity in a mixed system, no definite conclusion could be reached regarding the effect of varying light/dark cycles of medium frequency (seconds to minutes). It appeared as though the ratio of the euphotic depth to mixing depth (Z _eu/Z _m) influenced productivity more than the duration of the light/dark cycle. The static bottle incubation method gave higher integral productivities than the mixed samples at low ratio's ofZ _eu/Z _m. It is suggested that mixing has two separate, but synergistic effects i.e. it not only moves the phytoplankton cells through a light/dark cycle, but also decreases the boundary layer, which increases the rate of exchange through the cell wall of nutrients and metabolites. In doing so more nutrients are available and light could be utilized more efficiently and therefore, productivity is increased.     

Response of three phytoplankton bioassay techniques in experimental ponds of known limiting nutrient

In a controlled enrichment study of eight experimental ponds, results from the batch bioassay, primary productivity incubation bioassay, and chemostat techniques for measuring limiting factors of phytoplankton algae were compared to the change in the natural system with nutrient addition. In the ponds, rapid and dramatic increase in both phytoplankton biomass and primary productivity upon the addition of nitrogen and phosphorus fertilizer offered conclusive evidence that these nutrients were limiting in the control ponds to which no nutrients were added. Both the batch bioassay and chemostat techniques clearly indicated nitrogen and possibly phosphorus as the limiting factors; however, the primary productivity incubation bioassay technique showed no increase in ¹⁴C uptake with addition of these nutrients. A species- and/or nutrient-specific time lag between nutrient uptake and increased carbon fixation is suggested to explain the failure of the technique to yield positive results within the 4-hour incubation period used.     

滩涂池塘生态系统的光合能量利用及其影响因子

研究了江苏沿海滩涂不同养殖模式下初级生产力特征、能量利用率及其影响因子.结果表明,滩涂养殖水体浮游植物的初级生产力存在垂直分布、日变化和季节的动态变化,这种变化与太阳辐射强度有直接的关系.滩涂养殖水体净初级生产量平均值为(7.58±2.52) gO2/(m2 · d),只占毛产量的55.41%,太阳能转化为鲢、鳙鱼产量的生态效率为0.020%～0.029%,这些指标低于我国高产养殖塘,鱼塘生态系统结构有待进一步优化.浮游植物生物量、透明度、N/P值、Mg2+浓度等因素显著影响水体初级生产力的大小,多元逐步回归表明浮游植物叶绿素含量和到达水体表面的太阳辐射强度是初级生产力的最主要因子,其回归方程为:Pg =0.0785Cha+0.0577 Iλ0+1.346;n=22, FCha>F Iλ0>F0.1,而盐度、Na+、Cl-浓度影响不显著.     

Estimation of Primary Productivity by Chlorophyll a in vivo Fluorescence in Freshwater Phytoplankton

Primary productivity in marine waters is widely estimated by the measurements of ¹⁴C incorporation, the underwater light climate, and the absorption spectra of phytoplankton. In bio-optical models the quantum efficiency of carbon fixation derived from ¹⁴C incorporation rates, the photosynthetically absorbed radiation derived from the underwater light climate, and the phytoplankton absorption spectra are used to calculate time- and depth-integrated primary productivity. Due to the increased sensitivity of commercially available fluorometers, chlorophyll a in vivo fluorescence became a new tool to assess the photosynthetic activity of phytoplankton. Since fluorescence data yield only relative photosynthetic electron transport rates, a direct conversion into absolute carbon fixation rates is not possible. Here, we report a procedure how this problem can be adressed in freshwater phytoplankton. We adapted a marine bio-optical model to the freshwater situation and tested if this model yields realistic results when applied to a hypertrophic freshwater reservoir. Comparison of primary productivity derived from ¹⁴C incorporation to primary productivity derived from Chl a fluorescence showed that the conversion of fluorescence data into carbon fixation rates is still an unsolved problem. Absolute electron transport rates calculated from fluorescence data tend to overestimate primary production. We propose that the observed differences are caused mainly by neglecting the package effect of pigments in phytoplankton cells and by non-carbon related electron flow (e.g., nitrogen fixation). On the other hand, the ¹⁴C incorporation rates can be artificially influenced by "bottle effects", especially near the water surface, where photoinhibition, photorespiration, and Mehler reaction can play a major role.     

Effects of light history on primary productivity in a phytoplankton community dominated by the toxic cyanobacterium Cylindrospermopsis raciborskii

1. The effects of instantaneous irradiance and short‐term light history on primary production were determined for samples from a subtropical water reservoir dominated by the toxic cyanobacterium Cylindrospermopsis raciborskii. ¹⁴C‐bicarbonate uptake incubations were conducted on water samples from the reservoir, for irradiance (photosynthetically active radiation) ranging from 0 to 1654 μmol quanta m⁻² s⁻¹. Prior to the ¹⁴C incubations, cells were pre‐treated at irradiance levels ranging from 0 to 1006 μmol quanta m⁻² s⁻¹. 2. The average irradiance experienced by cells during the 2–2.5 h pre‐treatment incubations affected the productivity–irradiance (P–I) parameters: exposure to high light in pre‐treatment conditions caused a substantial decrease in maximum rate of primary production P_max and the photoinhibition parameter β when compared to cells pre‐treated in the dark. 3. While the data collected in this study were not sufficient to develop a full dynamic model of C. raciborskii productivity, P_max and β were modelled as a function of pre‐treatment irradiance, and these models were applied to predict the rate of primary production as a function of both instantaneous and historical irradiance. The results indicated that while cells with a history of exposure to high irradiance will be the most productive in high irradiance, production rates will be highest overall for dark‐acclimated cells in moderate irradiance. 4. Our results may explain why optically‐deep mixing favours C. raciborskii. If the mixing depth z_m exceeds the euphotic depth z_eu, cells will be dark‐acclimated, which will increase their rate of production when they are circulated through the euphotic zone. These results also predict that production rates will be higher during morning hours than for the same irradiance in the afternoon, which is consistent with other phytoplankton studies. 5. Since the rate of production of C. raciborskii‐dominated systems cannot be described by a single P–I curve, accurate estimates of production rates will require measurements over the daily light cycle.     

Primary and new production in the deep Canada Basin during summer 2002

The NOAA Ocean Exploration program provided the opportunity to measure the carbon and nitrogen productivity across the Canada Basin. This research examined the major environmental factors limiting the levels of primary production and possible future climate change on the ecosystems. The vertical distributions of the carbon and nitrogen uptakes of phytoplankton had similar patterns as their respective biomass concentrations which were low at the surface and highest in the chlorophyll-maximum layer. The annual carbon and new production rates of phytoplankton in the Canada Basin were about 5 and 1 g C m^–2, respectively. Nutrients were determined to be a main limiting factor at the surface, whereas light may be a major factor limiting phytoplankton productivity in the chlorophyll-maximum layer for open waters. The bottom surface of the ice has a low specific uptake and productivity of phytoplankton, indicating that photosynthetic activity might be controlled by both light and nutrients.     

Effects of fertiliser and crayfish on plankton and nutrient dynamics in hardwater ponds

Although phosphorus fertilisation can improve productivity in most freshwater ponds, phosphate may become limiting in extremely hard water due to rapid precipitation with calcium. Hence we studied the characteristics of plankton and nutrient dynamics in water containing >400 mg CaCO₃ l^–1in pond and microcosm systems. The field experiment was conducted in eight earthen ponds involving two nutrient ratios (N:P = 1:1 and 20:1) with or without crayfish. Fertilisation significantly increased concentrations of NO₂–N and NO₃–N, but soluble reactive phosphorus was depleted to the level prior to fertilisation within 24 h. The laboratory test showed that after 6 h of fertilisation, 45% phosphorus was precipitated by calcium, 30% phosphorus was assimilated by phytoplankton and only 25% phosphorus remained in water column. The phytoplankton abundance in hardwater ponds was regulated by the abundance of zooplankton population rather than by either crayfish or fertilisation. The presence of crayfish only increased the concentration of total phosphorus. This study suggests that when phytoplankton production is required in crayfish ponds the maintenance of phytoplankton abundance will depend on the effective control of zooplankton rather than fertilisation. Due to the rapid precipitation of phosphorus by calcium in hard water ponds, more frequent phosphorus fertilisation is needed to enhance primary productivity.     

Some effects of light on algal respiration and the validity of the light and dark hottle technique for measuring primary productivity

SUMMARY. Measurements of the rate of oxygen uptake in a number of blue-green algae and diatoms were carried out under both field and laboratory conditions to determine the effects of light on such rates. The light history of algal cells was an important controlling factor of oxygen uptake. When measured in the light, with dichlorophenyl-dimethylurea (DCMU), oxygen uptake was sometimes different from uptake measured in the dark. The results cast some doubt on the validity of the light and dark bottle method for determining primary productivity. It is suggested that oxygen uptake measurements should be made in the presence of DCMU.