泡果荠属(十字花科)五新种

长柱泡果荠 图1 Hilliella longistyla Y.H.Zhang,sp.nov. Haec species proxima H.changhuaensi Y.H.Zhang,a qua racemis longio-ribus,laxioribus,siliculis longe obovoideis,stylis longioribus,sub fructu c.2mmlongis,seminibus superificie tuberculis minoribus dense munitis recedit. Herba annua.Caulis erectus,c.42cm vel ultra altus,2.5—3mm crassus,striatus,nodis hispidulis.Folia caulina mediana et suprema trifoliolata;foliolamembranacea vel chartacea,subtus et supra ad nervos strigosa,nervis lateralibus 5     

湖南十字花科一新种

双牌泡果荠 新种 图1 砍莱(俗称) Hilliella shuangpaiensis Z. Y. Li, sp. nov. Species H. alatipedi (Hand. -Mazz.) Y. H. Zhang et H. W. Li et H. paradoxae (Hance) Y. H. Zhang et H. W. Li affinis, a qua foliis basalibus pinnatis 8—9-foliolatis, floribus minoribus, sepalis petalo subacquilongis, siliculis orbicularibus, 2 (-3) ovulatis differt.     

关于浙江泡果荠和棒毛荠的分类问题

再次对浙江泡果荠Hilliella warburgii和棒毛荠Cochleariella zhejiangensis作比较研究后,本文认为：两者差别明显,尤其是后者子房和果实表面密被发达的膜质棒状毛,又有各自不同的地理分布区,显然是不同属植物。棒毛荠属(单型属)的属名模式是Cochleariella zhejiangensis Y．H．Zhang et R．Vogt。     

安徽泡果荠属(十字花科)一新种

黟县泡果荠 新种 图版1:A Hilliella yixianensis Y. H. Zhang, sp. nov. H. paradoxa (Hance) Y. H. Zhang et H. W. Li ab specie nova recedit foliolis ma-joribus, pedicellis brevioribus c. 2 mm longis sub fructu 2—3 mm longis, sepalis navicu-laribus majoribus c. 2 mm longis 1 mm latis, petalis longioribus (2. 8—3. 3 mm longis), sili-culis valvis irregularibus interdum praeditis.     

浙江泡果荠一新变种

本文发表浙江泡果荠一新变种即白花浙江泡果荠Hilliella warburgii (O.E.Schulz) Y.H.Zhang et H.W.Li var,albiflora,sp.nov.     

十字花科一新属——泡果荠属

岩荠属泡果荠组植物(Cochlearia L.Sect.Hilliella O.E.Schulz)不仅在体态上而且在其它一系列特征特别是具泡状突起的果实特征上极不同于狭义的岩荠属(Cohc-learia L.s.str.),松蓝叶岩荠属[Glaucocochlearia(O.E.Schulz)Pobed.],以及拟常绿岩荠属[Pseudosempervium(Boiss)Grossh.]。这些特征看来可以作为建立一新属的根据,因此将其提升为一独立的属并把这一组的8个种组合到新属中去。     

湖南广西泡果荠属一新种

湖南广西泡果荠属一新种张渝华（浙江省医学科学院药物研究所，杭州３１００１３）ＡＮＥＷＳＰＥＣＩＥＳＯＦＨＩＬＬＩＥＬＬＡＦＲＯＭＨＵＮＡＮＡＮＤＧＵＡＮＧＸＩＺｈａｎｇＹｕｈｕａ（ＩｎｓｔｉｔｕｔｅｏｆＭａｔｅｒｉａＭｅｄｉｃａ，ＺｈｅｊｉａｎｇＡｃ...     

阴山荠属和泡果荠属植物染色体数目及过氧化物酶同工酶的比较

阴山荠属和泡果荠属受试种类的染色体数目是：柔毛阴山荠（Ｙ．ｈｅｎｒｙｉ（Ｏｌｉｖ．）Ｙ．Ｈ．Ｚｈａｎｇ）２ｎ＝１２,叉毛阴山荠（Ｙ．ｆｕｒｃａｔｏｐｉｌｏｓａ（Ｋｕａｎ）Ｙ．Ｈ．Ｚｈａｎｇ）２ｎ＝１２,双牌泡果荠（Ｈ．ｓｈｕａｎｇｐａｉｅｎｓｉｓＺ．Ｙ．Ｌｉ）２ｎ＝４４,黟县泡果齐（Ｈ．ｙｉｃｉａｎｅｎｓｉｓＹ．Ｈ．Ｚｈａｎｇ）２ｎ＝４２,双牌泡果（Ｈ．ｐａｒｄｏｘａ（Ｈａｎｃｅ）Ｙ．Ｈ．Ｚｈａｎ     

十字花科2个种的染色体数目

棒毛荠 Cochleariella zhejiangensis (Y. H. Zhang) Y. H. Zhang et R. Vogt与浙江泡果荠 H illiella warburgii (O. E. Schulz) Y. H. Zhang et H. W. Li同属十字花科 ,亲缘相近。前者因果瓣具十分独特的棒状毛和其它一些特征而独立成属 [1 ,2 ] 。后者原在广义岩荠属 Cochlearia L.中东亚分布的一个组中 ,该组后经整理扩大提升成泡果荠属 [3]。对于该 2种的分类归属 ,争议颇多。赵一之 [4]认为两者差别不大 ,将前者作为后者的变种更为恰当 ,并将棒毛荠属、泡果荠属与阴山荠属合并。陆莲立 [5]认为两者果瓣上的差别是同一植物种…     

Studies on the Structures of Two New Triterpene Saponins C and D from Polygala japoni-ca Houtt

Two new triterpene saponins C and D have been isolated from the aerial parts of Polygala japonica Houtt. Their molecular formulas: C42H68O15 were structural isomers of each other. Acid hydrolysis of the two saponins all produced a sapogenin (2a, 3a, 24-trihydroxyo-lean-12-ene-28-oic acid) and D-glucoses. But only the saponin D could be hydrolyzed in the alkaline solution, the products were identical with those from acid hydrolysis. Their structures have been established by means of 1HNMR,13CNMR and MS as 3-O-[β-D-glucopyranosyl(l→2)β-D-glucopyranosyl] 2α, 3α, 24-trihydroxyolean-12-ene-28-oic acid, 28-O-[β-D-glucopy-ranosyl (1→2)-β-D-glucopyranosyl] 2α, 3α, 24-trihydroxyolean-12-ene-28-oic acid.     

Study on the Structure of a New Triterpene Saponin B from Polygala japoniea Houtt

A new triterpene saponin B has been isolated from the earial parts of Polygala japonica Houtt in folk-lore medicine. Its molecular: C48H78O20, m.p. 199–202℃, [α]D23+30.0 (C, 0.5, CH3OH). Acidic hydrolysis of this saponin gave a sapogenin (2α, 3α, 24-tri-hydroxyolean-12-ene-28-oic acid) and D-glucose. The structure of saponin B was elucidated as 28-O- [β-D-glucopyranosyl (1→2) -β-D-glucopyranosyl (1→2) -β-D-glucopy- ranosyl] 2α, 3α, 24-trihydroxyolean-12-ene-28-oic acid mainly by 13C-NMR, MS and some chemical transfomations.     

Structure of the O-specific polysaccharide from a marine bacterium Oceanisphaeralitoralis KMM 3654(T) containing ManNAcA

The O-specific polysaccharide was isolated from the lipopolysaccharide of a marine bacterium Oceanisphaeralitoralis KMM 3654(T) and studied by chemical methods along with (1)H and (13)C NMR spectroscopy. The following new structure of the O-specific polysaccharide of O. litoralis containing D-glucose and two residues of 2-acetamido-2-deoxy-D-mannuronic acid was established: →4)-α-D-Glcp-(1→4)-β-D-ManpNAcA-(1→4)-β-D-ManpNAcA-(1→.     

野桂花化学成分研究

从野桂花（Osmanthus yunnanensis）地上部分95％乙醇提取物中首次分离得到18个化合物,应用波谱方法及与已知品对照的手段鉴定它们为：E-阿魏酸二十烷基酯（1）、β-谷甾醇（2）、羽扇豆醇（3）、齐墩果酸（4）、7-oxo-β—sitosterol（5）、乙酰齐墩果酸（6）、（6＇-O－palmitoyl）－sitosterol-3-O-β—D—glucoside（7）、rotundioic acid（8）、地榆糖甙II（9）、3β-hydroxy－27-p－（E）－eoumaroyloxyolean－12－en-28－oicacid（10）、3β—laydroxy-27-p－（Z）－coumaroyloxy－olean－12－en-28-oicacid（11）、hycandinic acid ester（12）、绿原酸丁酯（13）、4,5-二咖啡酰奎尼酸丁酯（14）、28-O-β-D—glueopyranosyl rottmdioic acid（16）以及三个半萜类化合物：4,5-dihydroxyprenyl caffeate（15）、4-（6-O-caffeoyl -β-D—glucopyranosyloxy）-5-hydroxyprenyl caffeate（17）、4-β-D—glucopyranosyloxy5-hydroxyprenyl caffeate（18）。     

广东紫珠中的萜类化合物及其抗炎活性

对岭南药材广东紫珠(Callicarpa kwangtungensis)地上部分进行化学成分研究,得到11个萜类化合物,分别鉴定为sambucunlin A(1)、2α-羟基羽扇豆醇(2)、swinhoeic acid(3)、3β-羟基-乌苏烷-11-烯-13β,28-内酯(4)、蔷薇酸(5)、2α,3β,6β,18β,23-pentahydroxy-olean-12-en-28-oic acid(6)、rel-5-(3 S,8 S-dihydroxy-1 R,5 S-dimethyl-7-oxa-6-oxobicyclo-oct-8-yl)-3-methyl-2 Z,4 E-pentadienoic acid(7)、salvionoside B(8)、齐墩果酸(9)、白桦脂酸(10)和α-香树脂醇(11)。其中,化合物1~4和6~8为首次从该属植物中分离得到。在化学成分分离基础上,进一步选择脂多糖(LPS)诱导的RAW 264.7小鼠巨噬细胞炎症模型进行萜类化合物抗炎活性测试。结果表明:化合物3和9具有显著的抗炎活性,对比结构发现,三萜类化合物(3~6、9和11)抗炎活性优于倍半萜类化合物(7和8)。研究结果广东紫珠萜类成分在抗炎方面的临床应用提供了实验依据。     

Detection of Shiga toxin-producing Escherichia coli serotypes O26:H11, O103:H2, O111:H8, O145:H28, and O157:H7 in raw-milk cheeses by using multiplex real-time PCR

Shiga toxin (Stx)-producing Escherichia coli (STEC) strains are a diverse group of food-borne pathogens with various levels of virulence for humans. In this study, we describe the use of a combination of multiple real-time PCR assays for the screening of 400 raw-milk cheeses for the five main pathogenic STEC serotypes (O26:H11, O103:H2, O111:H8, O145:H28, and O157:H7). The prevalences of samples positive for stx, intimin-encoding gene (eae), and at least one of the five O group genetic markers were 29.8%, 37.3%, and 55.3%, respectively. The H2, H7, H8, H11, and H28 fliC alleles were highly prevalent and could not be used as reliable targets for screening. Combinations of stx, eae variants, and O genetic markers, which are typical of the five targeted STEC serotypes, were detected by real-time PCR in 6.5% of the cheeses (26 samples) and included stx-wzx(O26)-eae-β1 (4.8%; 19 samples), stx-wzx(O103)-eae-ε (1.3%; five samples), stx-ihp1(O145)-eae-γ1 (0.8%; three samples), and stx-rfbE(O157)-eae-γ1 (0.3%; one sample). Twenty-eight immunomagnetic separation (IMS) assays performed on samples positive for these combinations allowed the recovery of seven eaeβ1-positive STEC O26:H11 isolates, whereas no STEC O103:H2, O145:H28, or O157:H7 strains could be isolated. Three stx-negative and eaeβ1-positive E. coli O26:[H11] strains were also isolated from cheeses by IMS. Colony hybridization allowed us to recover STEC from stx-positive samples for 15 out of 45 assays performed, highlighting the difficulties encountered in STEC isolation from dairy products. The STEC O26:H11 isolates shared the same virulence genetic profile as enterohemorrhagic E. coli (EHEC) O26:H11, i.e., they carried the virulence-associated genes EHEC-hlyA, katP, and espP, as well as genomic O islands 71 and 122. Except for one strain, they all contained the stx1 variant only, which was reported to be less frequently associated with human cases than stx2. Pulsed-field gel electrophoresis (PFGE) analysis showed that they displayed high genetic diversity; none of them had patterns identical to those of human O26:H11 strains investigated here.     

Transformation of 1- and 2-methylnaphthalene by Cunninghamella elegans.

Cunninghamella elegans metabolized 1- and 2-methylnaphthalene primarily at the methyl group to form 1- and 2-hydroxymethylnaphthalene, respectively. Other compounds isolated and identified were 1- and 2-naphthoic acids, 5-hydroxy-1-naphthoic acid, 5-hydroxy-2-naphthoic acid, 6-hydroxy-2-naphthoic acid, and phenolic derivatives of 1- and 2-methylnaphthalene. The metabolites were isolated by thin-layer and reverse-phase high-pressure liquid chromatography and characterized by the application of UV-visible absorption, 1H nuclear magnetic resonance, and mass spectral techniques. Experiments with [8-14C]2-methylnaphthalene indicated that over a 72-h period, 9.8% of 2-methylnaphthalene was oxidized to metabolic products. The ratio of organic-soluble in water-soluble metabolites at 2 h was 92:8, and at 72 h it was 41:59. Enzymatic treatment of the 48-h aqueous phase with either beta-glucuronidase or arylsulfatase released 60% of the metabolites of 2-methylnaphthalene that were extractable with ethyl acetate. In both cases, the major conjugates released were 5-hydroxy-2-naphthoic acid and 6-hydroxy-2-naphthoic acid. The ratio of the water-soluble glucuronide conjugates to sulfate conjugates was 1:1. Incubation of C. elegans with 2-methylnaphthalene under an 18O2 atmosphere and subsequent mass spectral analysis of 2-hydroxymethylnaphthalene indicated that hydroxylation of the methyl group is catalyzed by a monooxygenase.     

Effects of common forage phenolic acids on Escherichia coli O157:H7 viability in bovine feces

Ruminant animals are carriers of Escherichia coli O157:H7, and the transmission of E. coli O157:H7 from cattle to the environment and to humans is a concern. It is unclear if diet can influence the survivability of E. coli O157:H7 in the gastrointestinal system or in feces in the environment. Feces from cattle fed bromegrass hay or corn silage diets were inoculated with E. coli O157:H7, and the survival of this pathogen was analyzed. When animals consumed bromegrass hay for <1 month, viable E. coli O157:H7 was not recovered after 28 days postinoculation, but when animals consumed the diet for >1 month, E. coli O157:H7 cells were recovered for >120 days. Viable E. coli O157:H7 cells in feces from animals fed corn silage were detected until day 45 and differed little with the time on the diet. To determine if forage phenolic acids affected the viability of E. coli O157:H7, feces from animals fed corn silage or cracked corn were amended with common forage phenolic acids. When 0.5% trans-cinnamic acid or 0.5% para-coumaric acid was added to feces from silage-fed animals, the E. coli O157:H7 death rate was increased significantly (17-fold and 23-fold, respectively) compared to that with no addition. In feces from animals fed cracked corn, E. coli O157:H7 death rates were increased significantly with the addition of 0.1% and 0.5% trans-cinnamic acid (7- and 13-fold), 0.1% and 0.5% p-coumaric acid (3- and 8-fold), and 0.5% ferulic acid (3-fold). These data suggest that phenolic acids common to forage plants can decrease viable counts of E. coli O157:H7 shed in feces.     

In vitro lipid metabolism in the rat pancreas. II. Effects of secretagogues on fatty acid metabolism, net lipolysis and ATP levels.

1. The concentration of carbamylcholine, bombesin, pancreozymin, pentagastrin and secretin evoking a similar 4--5-fold maximal increase in amylase secretion from rat pancreatic fragments were 3.10(-6), 10(-7), 10(-8), 3.10(-6), and 3.10(-6) M, respectively. The maximal concentration of vasoactive intestinal peptide tested (3.10(-6) M) increased amylase secretion by 250%. The six secretagogues could be separated into two groups according to their effects on lipid metabolism and ATP levels. 2. When used at their optimal concentrations, carbamylcholine, bombesin, pancreozymin, and pentagastrin lowered pancreatic ATP levels by 18-26% and increased net release of free fatty acids by 68-105%. 3. The effects of 3.10(-6) M carbamylcholine and 10(-8) M pancreozymin on the metabolism of 3H2O, D-[U-14C]glucose and [1-14C]acetate were similar; the incorporation of radioactivity in the fatty acid moiety of glycerolipids decreased by 20--50% whereas the incorporation of 3H from 3H2O and of 14C from [U-14C]glucose increased by 20--35% in the glycerol moiety. In addition, the oxidation of [U-14C]glucose, [1-14C]acetate and [1-14C]palmitate to 14CO2 increased by 15--32% while the esterification of [1-14C]palmitate, [1-14C]-linoleate, and [1-14C]arachidonate was inhibited by 14--23%. The spectrum of fatty acids labeled with [1-14C]acetate indicated an inhibition of the malonic acid pathway whereas the elongation of polyenoic fatty acids was unaltered.     

Peroxisomal fatty acid oxidation as detected by H2O2 production in intact perfused rat liver.

1. H2O2 formation associated with the metabolism of added fatty acids was quantitatively determined in isolated haemoglobin-free perfused rat liver (non-recirculating system) by two different methods. 2. Organ spectrophotometry of catalase Compound I [Sies & Chance (1970) FEBS Lett. 11, 172-176] was used to detect H2O2 formation (a) by steady-state titration with added hydrogen donor, methanol or (b) by comparison of fatty-acid responses with those of the calibration compound, urate. 3. In the use of the peroxidatic reaction of catalase, [14C]methanol was added as hydrogen donor at an optimal concentration of 1 mM in the presence of 0.2 mM-L-methionine, and 14CO2 production rates were determined. 4. Results obtained by the different methods were similar. 5. The yield of H2O2 formation, expressed as the rate of H2O2 formation in relation to the rate of fatty-acid supply, was less than 1.0 in all cases, indicating that, regardless of chain length, less than one acetyl unit was formed per mol of added fatty acid by the peroxisomal system. In particular, the standard substrate used with isolated peroxisomal preparations (C16:0 fatty acid) gave low yield (close to zero). Long-chain monounsaturated fatty acids exhibit a relatively high yield of H2O2 formation. 6. The hypolipidaemic agent bezafibrate led to slightly increased yields for most of the acids tested, but the yield with oleate was decreased to one-half the original yield. 7. It is concluded that in the intact isolated perfused rat liver the assayable capacity for peroxisomal beta-oxidation is used to only a minor degree. However, the observed rates of H2O2 production with fatty acids can account for a considerable share of the endogenous H2O2 production found in the intact animal.