异型兰的种子试管育苗

1植物名称异型兰(Chiloschista yunnanensisSchltr.)。2材料类别种子。3培养条件种子萌发培养基:(1)MS;(2)1/2MS(大量、微量元素用量减半);(3)3g·L-1花宝1号(美国Hyponex公司,N:P:K=7:6:19);(4)MS+100mL·L-1椰子乳;(5)1/2MS+100mL·L-1椰子乳;(6)3g·L-1花宝1号+100mL·L-1椰子乳;(7)1/2MS+100mL·L-1椰子乳+1g·L-1蛋白胨;(8)1/2MS+100mL·L-1椰子乳+1g·L-1胰蛋白胨;(9)1/2MS+100mL·L-1椰子乳+1g·L-1酵母提取液。生根育苗培养基:(10)3g·L-1花宝1号+2g·L-1活性炭+100g·L-1香蕉汁;(11)3g·L-1花宝1号+2g·L-1活性炭…     

多花脆兰的离体快速繁殖

1 植物名称多花脆兰[Acampe rigida(Buch.-Ham.ex J.E.Smith)P.F.Hunt]. 2 材料类别种子. 3 培养条件种子萌发培养基:(1)MS;(2)1/2MS(大量元素用量减半);(3)KC[成份如下:MgSO4·7H2O 250 mg·L-1(单位下同);KH2PO425O;(NH4)2SO4 500; Ca(NO 3)2·2H2O 1 000 ;FeSO4·7H2O 25; MnSO4.4H2O 7.5;肌醇(C6H12O6)100;盐酸硫胺素(VB,)1; D-甘露糖醇12.7%];(4)1 g·L-1花宝1号(美国Haponex公司产品,N:P:K=7:16:9)+2 g.L-1花宝2号(美国Haponex公司产品,N:P:K=20:20:20);(5)MS+100 g·L-1香蕉汁;(6)1/2MS+100 g·L-1香蕉汁;(7)KC+100 g·L-1香蕉汁;(8)1 g·L-1花宝1号+2 g·L-1花宝2号+100g·L-1香蕉汁.原球茎增殖和分化成苗培养基:(9)1/2MS+6.BA 1.5+NAA 0.2+100 g·L-1香蕉汁;(10)1/2MS+6-BA 0.5+NAA 0.05+100 g·L-1香蕉汁;(11)1 g·L-1花宝1号+2 g·L-1花宝2号+6-BA 1.0+NAA0.5+100 g·L-1香蕉汁.     

虎杖的组织培养与快速繁殖

以虎杖茎段、叶柄、叶片为外植体探讨了愈伤组织诱导、分化和植株再生的条件,筛选出茎段生长培养基为1/2MS+BA1.0mg·L-1+KT0.5mg·L-1+NAA0.2mg·L-1,茎段、叶柄和叶片外植体愈伤组织诱导培养基为MS+BA1.0～2.0mg·L-1+KT0.2～0.5mg·L-1+NAA0.2～0.5mg·L-1或MS+BA2.0～3.0mg·L-1+KT0.2～0.5mg·L-1+2,4-D0.5mg·L-1;丛生芽诱导培养基为MS+BA2.0mg·L-1+KT0.5mg·L-1+IBA0.2mg·L-1+LH1000;不定根及根状茎诱导培养基为1/2MS+IBA0.2mg·L-1.     

白旗兜兰种子非共生萌发与试管苗快速繁育

对我国极小种群野生植物白旗兜兰的种子非共生萌发与试管苗快速繁育开展研究,对比了不同培养基、预处理、种子成熟度及光照对种子萌发的影响。白旗兜兰种子萌发的最适条件是授粉后270 d的种子,在1/4MS+10%椰汁培养基上,用1%Na OCl溶液处理种子40 min并在播种后的前4周进行黑暗培养。最适合原球茎转化幼苗的培养基是3.0 g·L-1花宝一号+1.0 mg·L-1NAA+0.5 g·L-1活性炭+10%香蕉匀浆,而最佳幼苗生根壮苗的培养基是3.0 g·L-1花宝一号+1.0 mg·L-1NAA+1.0 mg·L-16-BA+0.5 g·L-1活性炭+10%香蕉匀浆。     

带叶兜兰的无菌播种和离体快速繁殖

1植物名称带叶兜兰(PaphiopedilumhirsutissimumPfitz)。2材料类别种子。3培养条件种子萌发培养基:(1)RE(Arditti1982);(2)VW;(3)1/4MS;(4)MS;(5)RE+活性炭2g·L-1;(6)RE;(7)RE+活性炭2g·L-1;(8)花宝1号(美国Haponex公司产品,N∶P∶K=7∶6∶19)2.5g·L-1。原球茎增殖和分化成苗培养基:(9)RE+6-BA2.0mg·L-1(单位下同)+NAA0.2;(10)RE+活性炭2g·L-1+6-BA2.0+NAA0.2;(11)花宝1号1.5g·L-1+花宝2号(美国Haponex公司产品,N∶P∶K=20∶20∶20)1.5g·L-1+活性炭2g·L-1+6-BA2.0+NAA0.2。生根壮苗培养基:(12)RE+NAA1.0+活…     

蒙花忍冬的组织培养与快速繁殖研究

以金银花 蒙花 品系的茎段为外植体 ,于 MS和 B5附加不同激素配比的培养基上 ,探讨愈伤组织形成和丛生芽诱导及生根培养的条件 .诱导愈伤组织和丛生芽的培养基为 B5+BA2 .0 m g· L- 1 +KT0 .2～ 0 .5 mg· L- 1+IAA 0 .5～ 1 .0 mg· L- 1 +L H 1 0 0 0 m g· L- 1 ,继代增殖培养的培养基为 B5+BA 2 .0 m g· L- 1 +KT 0 .5 m g·L- 1 +IAA1 .0 m g· L- 1 +L H1 0 0 0 m g· L- 1和 B5+BA2 .0 mg· L- 1 +KT0 .36 m g· L- 1 +IAA1 .0 mg· L- 1+CH 1 0 0 0 m g· L- 1 +1 / 2 MS :大量元素 ,生根培养基为 1 / 2 MS +IBA 0 .2 mg· L- 1 +L H 1 0 0 0 mg·L- 1 .结果表明 L H和 CH在金银花愈伤组织和丛生芽诱导方面具明显的作用     

细茎石斛的快速繁殖和试管开花诱导

1植物名称细茎石斛[Dendrobiummoniliforme(L.)Sw.]。2材料类别野生植株的成熟种子。3培养条件种子萌发培养基:(1)1/2MS+NAA0.5mg·L-1(单位下同);增殖分化培养基:(2)1/2MS+NAA0.4;生根壮苗培养基:(3)1/2MS+NAA0.2+6-BA2.0;试管开花预处理培养基:(4)1/2MS+TDZ0.05,(5)1/2MS+PP3330.40+ABA1.0,预处理90d;试管开花培养基:(6)MS(1/6N,2P,2K)+PP3331.0+TDZ0.1。每个处理20株,重复3次,150d内统计花芽诱导率(花芽数/植株数)和正常花开放率(正常花开放数/植株数)。以上培养基均附加4%蔗糖、10%(W/V)香蕉汁、8g·L-1琼脂、0.1g·…     

土人参茎尖培养和植株再生

1　植物名称　土人参 (Talinumpaniculatum)。2　材料类别　实生苗之茎尖 ,种子取自本校花圃。3　培养条件　种子萌发培养基 :( 1 ) 1 /2MS +0 .7%琼脂 + 2 %蔗糖。丛生芽诱导培养基 :( 2 )MS + 6 BA 0 .5mg·L- 1 (单位下同 ) ;( 3)MS +NAA0 .5 + 6 BA 2 .0 ;( 4 )MS +NAA 0 .5 +KT 2 .0 ;( 5 )MS+NAA 0 .5 + 6 BA 3.0。丛生芽增殖培养基 :( 6)MS + 6 BA 2 .0 ;( 7)MS +NAA 0 .0 5 + 6 BA 3.0。生根培养基 :( 8) 1 /2MS +NAA 2 .0。上述 ( 2 )～ ( 8)培养基均附加 30 g·L- 1 蔗糖、7g·L- 1 琼脂 ,pH 5 .8。培养室温…     

火焰兰的种子培养和试管育苗

1植物名称火焰兰(Renanthera CoCCinea). 2材料类别种子. 3培养条件种子萌发培养基:(1)VW;(2)VW 100 mL·L-1椰子乳;(3)KC;(4)KC 100 mL·L-1椰子乳(5)1/2MS;(6)MS.生根育苗培养基:(7)3 g·L-1花宝1号(美国Haponex公司产品,N:P:K=7:6:19) 2 g·L-1蛋白胨 2 g·L-1活性炭 0.5 mg·L-1NAA 0.2 mg·L-16-BA;(8)1 g·L-1花宝1号 1 g·L-1花宝2号(N:P:K=20:20:20) 2 g·L-1蛋白胨 2 g·L-1活性炭 0.5 mg·L-1NAA 0.2 mg·L-16-BA.以上培养基均附加1.5%蔗糖、0.6%琼脂,pH 5.2～5.4.培养温度为(25±2)℃,光照度1 500～2000lx,光照时间12 h·d-1.     

羚羊石斛的离体快速繁殖

1植物名称羚羊石斛(DendrobiumcochliodesSchltr.)。2材料类别种子。3培养条件萌发培养基:(1)VW;(2)VW+椰子乳;(3)1/2MS;(4)1/2MS+椰子乳;(5)N6;(6)N6+椰子乳;(7)N6+椰子乳+活性炭2g·L-1。继代增殖和分化成苗培养基:(8)N6+椰子乳+6-BA1.0mg·L-1(单位下同)+NAA0.2;(9)N6+椰子乳+6-BA0.2+NAA0.1;(10)N6+椰子乳+6-BA0.2+NAA0.1+活性炭2g·L-1;(11)花宝1号(美国Haponex公司产品,N∶P∶K=7∶6∶19)1.5g·L-1+花宝2号(美国Haponex公司产品,N∶P∶K=20∶20∶20)1.5g·L-1+椰子乳+6-BA0.2+NAA0.1。生根壮苗培养基:(12)N6+NA…     

A Unique Protease Cleavage Site Predicted in the Spike Protein of the Novel Pneumonia Coronavirus (2019-nCoV) Potentially Related to Viral Transmissibility

正Dear Editor,In December 2019, a novel human coronavirus caused an epidemic of severe pneumonia(Coronavirus Disease 2019,COVID-19) in Wuhan, Hubei, China(Wu et al. 2020; Zhu et al. 2020). So far, this virus has spread to all areas of China and even to other countries. The epidemic has caused 67,102 confirmed infections with 1526 fatal cases     

Curcuminoids as inhibitors of thioredoxin reductase: A receptor based pharmacophore study with distance mapping of the active site

Curcumin is the yellow pigment of turmeric that interacts irreversibly forming an adduct with thioredoxin reductase (TrxR), an enzyme responsible for redox control of cell and defence against oxidative stress. Docking at both the active sites of TrxR was performed to compare the potency of three naturally occurring curcuminoids, namely curcumin, demethoxy curcumin and bis-demethoxy curcumin. Results show that active sites of TrxR occur at the junction of E and F chains. Volume and area of both cavities is predicted. It has been concluded by distance mapping of the most active conformations that Se atom of catalytic residue SeCYS498, is at a distance of 3.56 from C13 of demethoxy curcumin at the E chain active site, whereas C13 carbon atom forms adduct with Se atom of SeCys 498. We report that at least one methoxy group in curcuminoids is necessary for interation with catalytic residues of thioredoxin. Pharmacophore of both active sites of the TrxR receptor for curcumin and demethoxy curcumin molecules has been drawn and proposed for design and synthesis of most probable potent antiproliferative synthetic drugs.     

Comparative morphology of the carpel in the Liliaceae: Hewardieae, Petrosavieae, and Tricyrteae

The young pistils in the melanthioid tribes, Hewardieae, Petrosavieae and Tricyrteae, are uniformly tricarpellate and syncarpous. They lack raphide idioblasts. All are multiovulate, with bitegmic ovules. The Petrosavieae are marked by the presence of septal glands and incomplete syncarpy. Tepals and stamens adhere to the ovary in the Hewardieae and the Petrosavieae but not in the Tricyrteae. Two vascular bundles occur in the stamens of the Hewartlieae and Tricyrtis latifolia. Ventral bundles in the upper part of the ovary of the Hewardieae are continuous with compound septal bundles and placental bundles in the lower part. Putative ventral bundles occur in the alternate position in the Tricyrteae and putative placental bundles in the opposite. position in the Petrosavieae. The dichtomously branched stigma in each carpel of the Tricyrteae is supplied by a bifurcated dorsal bundle.     

Enterovirus 71 3C proteolytically processes the histone H3 N-terminal tail during infection

Highlights
1. The N-terminal tail of histone H3 is specifically cleaved during EV71 infection.
2. Viral protease 3C is identified as a protease responsible for proteolytically processing the N-terminal H3 tail.
3. Our finding reveals a new epigenetic regulatory mechanism for Enterovirus 71 in virus-host interactions.     

Detection of EBV and HHV6 in the Brain Tissue of Patients with Rasmussen’s Encephalitis

Rasmussen’s encephalitis (RE) is a rare pediatric neurological disorder, and the exact etiology is not clear. Viral infection may be involved in the pathogenesis of RE, but conflicting results have reported. In this study, we evaluated the expression of both Epstein-Barr virus (EBV) and human herpes virus (HHV) 6 antigens in brain sections from 30 patients with RE and 16 control individuals by immunohistochemistry. In the RE group, EBV and HHV6 antigens were detected in 56.7% (17/30) and 50% (15/30) of individuals, respectively. In contrast, no detectable EBV and HHV6 antigen expression was found in brain tissues of the control group. The co-expression of EBV and HHV6 was detected in 20.0% (6/30) of individuals. In particular, a 4-year-old boy had a typical clinical course, including a medical history of viral encephalitis, intractable epilepsy, and hemispheric atrophy. The co-expression of EBV and HHV6 was detected in neurons and astrocytes in the brain tissue, accompanied by a high frequency of CD8⁺ T cells. Our results suggest that EBV and HHV6 infection and the activation of CD8⁺ T cells are involved in the pathogenesis of RE.     

Perinatal Vertical Transmission of Chikungunya Virus in Ruili,a Town on the Border between China and Myanmar

正Dear Editor,Chikungunya virus (CHIKV), an arbovirus in the family of Togaviridae, genus Alphavirus, is transmitted by the A.aegyptii or A. albopictus mosquito, and causes disease in humans characterized by fever, rash, and arthralgia (Silva and Dermody 2017; Suhrbier 2019). It was first reported in 1953 in Tanzania, and caused only a few outbreaks and sporadic cases in Africa and Asia in last century. However, in the epidemic in 2004, CHIKV acquired mutations that conferred enhanced transmission by the A. albopictus mosquito(Schuffenecker et al. 2006). Since then, it has successively caused outbreaks in Africa, the Indian Ocean, South East Asia, the South America, and Europe (Zeller et al. 2016).     

Development of a Novel Reverse Transcription Loop-Mediated Isothermal Amplification Method for Rapid Detection of SARS-CoV-2

In conclusion, the novel visual RT-LAMP assay is a simple, rapid, and sensitive approach for detection of SARS-CoV-2, and it is ready for application in primary care and community hospitals or health care centers, and even patients' own houses in response to the current SARS-CoV-2 epidemic because the assay does not require sophisticated equipment and skilled personnel. Furthermore, it is also ready to be used in fields for screening samples from wild animals and environments to facilitate the identification of potential intermediate hosts that mediate the cross-species transmission of SARS-CoV-2 from bats to humans.