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1.
F. Wilde V. Korzun E. Ebmeyer H. H. Geiger T. Miedaner 《Molecular breeding : new strategies in plant improvement》2007,19(4):357-370
Fusarium head blight (FHB) is one of the most economically important wheat diseases, resulting in losses in grain yield and quality
as well as contamination with deoxynivalenol (DON). Cultivar Sumai 3 from China and its descendants as well as var. Frontana from Brazil have been identified as potent sources of resistance and subsequently mapped by molecular markers. The aim of
the present study was to compare phenotypic and marker-based selection in spring wheat. In a double cross, we combined two
donor-quantitative trat loci (QTL) alleles from CM82036 (Sumai 3/Thornbird) located on chromosomes 3B and 5A and one donor-QTL allele from var. Frontana on chromosome 3A with two high-yielding German spring wheat varieties. This initial population was selected phenotypically
by a two- (CP1) and three-step procedure (CP1+) and by independent marker-based analysis using one to three flanking markers per QTL (CM). To estimate selection gain, the
two phenotypically selected variants and the marker-selected variant as well as an unselected variant (C0) were inoculated
with FHB in the field at four locations in 2004. Between 26 and 135 progeny were tested from each variant. FHB severity and
DON content were significantly reduced by all selection variants. The highest total selection gain was obtained by the three-step
phenotypic selection for both traits, although marker-based selection for the two donor-QTL alleles from CM82036 proved to
be more powerful on an annual basis. The large range of variation for FHB resistance and, to a lesser extent, DON content
within the marker-based variant, however, shows that an additional phenotypic selection will enhance selection gain. 相似文献
2.
Somers DJ Thomas J Depauw R Fox S Humphreys G Fedak G 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2005,111(8):1623-1631
Fusarium head blight of wheat is a major deterrent to wheat production world-wide. The genetics of FHB resistance in wheat
are becoming clear and there is a good understanding of the genome location of FHB resistance QTL from different sources such
as Sumai3, Wuhan, Nyubai and Frontana. All the components needed for assembling complex genotypes through large-scale molecular
breeding experiments are now available. This experiment used high throughput microsatellite genotyping and half-seed analysis
to process four independent crosses through a molecular breeding strategy to introduce multiple pest resistance genes into
Canadian wheat. This included two backcrosses and selection for a total of six FHB resistance QTL, orange blossom wheat midge
resistance (Sm1) and leaf rust resistance (Lr21). In addition, the fixation of the elite genetic background was monitored with 45–76 markers to accelerate restoration of
the genetic background at each backcross. The strategy resulted in 87% fixation of the elite genetic background on average
at the BC2F1 generation and successfully introduced all of the chromosome segments containing FHB, Sm1 and Lr21 resistance genes. The molecular breeding strategy was completed in 25 months, at an equal pace to conventional crossing and
selection of spring wheat. 相似文献
3.
Nduulu LM Mesfin A Muehlbauer GJ Smith KP 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2007,115(4):561-570
Fusarium head blight (FHB) is a major disease of barley (Hordeum vulgare L.) that results in reduced grain yield and quality through the accumulation of the mycotoxin deoxynivalenol (DON). Coincident
QTL for FHB severity, DON concentration, and heading date (HD) map to a region of chromosome 2(2H) designated Qrgz-2H-8. It
is unclear whether disease resistance at this locus is due to a pleiotropic effect of late HD by delaying the host exposure
to the pathogen or a tightly linked resistance gene. The objectives of this study were to develop a set of near isogenic lines
(NILs) for the Qrgz-2H-8 region and to genetically dissect the QTL region containing the coincident traits. Two NIL populations
were developed consisting of F2- and F4-derived recombinants from a cross between a BC5 line carrying the donor parent (Chevron) alleles in the Qrgz-2H-8 region and the recurrent parent M69. Analysis of field
and marker data from these NILs revealed that the Chevron alleles conditioning FHB resistance, late HD, and low DON concentration
were successfully introgressed into the BC5 parent line and were segregating among NILs. QTL analysis of the F4-derived population showed that the HD QTL is adjacent to the FHB QTL. Furthermore, a single NIL was identified that was similar
to the resistant BC5 parent for FHB severity and the early flowering parent M69 for HD. These results indicate that the relationship between FHB
and HD at the Qrgz-2H-8 region is likely due to tight linkage rather than pleiotropy. 相似文献
4.
Molecular mapping of resistance to<Emphasis Type="Italic"> Fusarium</Emphasis> head blight in the spring wheat cultivar Frontana 总被引:13,自引:0,他引:13
Steiner B Lemmens M Griesser M Scholz U Schondelmaier J Buerstmayr H 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2004,109(1):215-224
Fusarium head blight (FHB) is a destructive disease of wheat. The objective of this study was to characterise the FHB resistance of the Brazilian spring wheat cultivar Frontana through molecular mapping. A population of 210 doubled-haploid lines from a cross of Frontana (partially resistant) and Remus (susceptible) was evaluated for FHB resistance during three seasons. Spray and single-spikelet inoculations were applied. The severity, incidence and spread of the disease were assessed by visual scoring. The population was genotyped with 566 DNA markers. The major QTL effect associated with FHB resistance mapped to chromosome 3A near the centromere, explaining 16% of the phenotypic variation for disease severity over 3 years. The most likely position is in the Xgwm720–Xdupw227 interval. The genomic region on 3A was significantly associated with FHB severity and incidence in all years evaluated, but not with FHB spread, indicating the prominent contribution of this QTL to resistance against initial infection. The map interval Xgwm129–Xbarc197 on chromosome 5A also showed consistent association with FHB severity and accounted for 9% of the phenotypic variation. In addition, smaller effects for FHB severity were identified on chromosomes 1B, 2A, 2B, 4B, 5A and 6B in single years. Individual QTLs for resistance to FHB spread accounted for less than 10% of the variation in trait expression. The present study indicates that FHB resistance of Frontana primarily inhibits fungal penetration (type I resistance), but has a minor effect on fungal spread after infection (type II resistance).Communicated by H.C. Becker 相似文献
5.
Wilde F Schön CC Korzun V Ebmeyer E Schmolke M Hartl L Miedaner T 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2008,117(1):29-35
Fusarium head blight (FHB) is one of the most important wheat diseases that causes yield and quality losses as well as contamination with deoxynivalenol (DON). This study aimed for marker-based introduction of three previously mapped QTLs from two German winter wheat resistance sources into an elite background unrelated to the mapping population. A double cross (DC) served as initial population that combined two resistance donor-QTL alleles from "Dream" (Qfhs.lfl-6AL, Qfhs.lfl-7BS) and one donor-QTL allele from "G16-92" on chromosome 2BL with two high yielding, susceptible elite winter wheats ("Brando", "LP235.1"). The initial population of 600 DC-derived F(1) lines was selected with SSR markers for the respective QTLs. After two marker-selection steps, each of eight marker classes was represented by 9-22 lines possessing the respective donor-QTL allele or all possible combinations thereof in the homozygous state. The effect of the QTLs was estimated by field tests at four locations inoculated with Fusarium culmorum. Resistance was measured as the mean of multiple FHB ratings (0-100%). Marker classes incorporating only one QTL were not significantly more resistant than the class without any QTL, the combination of two donor-QTL alleles reduced FHB significantly. On average, lines with Qfhs.lfl-6AL were significantly taller than lines without this QTL. A considerable variation for FHB resistance was found in all marker classes. Marker-based introduction of two QTLs enhanced mean FHB rating by about 40 percentage points, the selected plants, however, were, on average, significantly taller. Both findings strongly support a phenotypic selection following after marker-based introduction of effective QTLs. 相似文献
6.
Novel quantitative trait loci (QTL) for Fusarium head blight resistance in wheat cultivar Chokwang 总被引:6,自引:0,他引:6
Yang J Bai G Shaner GE 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2005,111(8):1571-1579
Fusarium head blight (FHB) is one of the most destructive diseases in wheat. This study was to identify new quantitative trait
loci (QTL) for FHB resistance and the molecular markers closely linked to the QTL in wheat cultivar Chokwang. The primers
of 612 simple sequence repeats (SSRs) and 12 target-region-amplified polymorphism (TRAP) marker were analyzed between resistant
(Chokwang) and susceptible (Clark) parents. One hundred and seventy-two polymorphic markers were used to screen a population
of 79 recombinant inbred lines (RILs) derived from the cross of Chokwang and Clark. One major QTL, Qfhb.ksu-5DL1, was identified on chromosome 5DL. The SSR marker Xbarc 239 was mapped in the QTL region, and also physically located to the bin of 5DL1-0.60-0.74 by using Chinese Spring deletion
lines. Another QTL Qfhb.ksu-4BL1was linked to SSR Xbarc 1096 and tentatively mapped on 4BL. A QTL on 3BS, Qfhb.ksu-3BS1, was also detected with marginal significance in this population. Different marker alleles for these QTL were detected between
Chokwang and Sumai 3 and its derivatives. These results suggested that Chokwang contains new QTL for FHB resistance that are
different from those in Sumai 3. Pyramiding resistance QTL from various sources may enhance FHB resistance in wheat cultivars. 相似文献
7.
Yu JB Bai GH Cai SB Ban T 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2006,113(2):308-320
The major quantitative trait locus (QTL) on 3BS from Sumai 3 and its derivatives has been used as a major source of resistance to Fusarium head blight (FHB) worldwide, but resistance genes from other sources are necessary to avoid complete dependence on a single source of resistance. Fifty-nine Asian wheat landraces and cultivars differing in the levels of FHB resistance were evaluated for type II FHB resistance and for genetic diversity on the basis of amplified fragment length polymorphism (AFLP) and simple sequence repeats (SSRs). Genetic relationships among these wheat accessions estimated by cluster analysis of molecular marker data were consistent with their geographic distribution and pedigrees. Chinese resistant landraces had broader genetic diversity than that of accessions from southwestern Japan. The haplotype pattern of the SSR markers that linked to FHB resistance quantitative trait loci (QTLs) on chromosomes 3BS, 5AS and 6BS of Sumai 3 suggested that only a few lines derived from Sumai 3 may carry all the putative QTLs from Sumai 3. About half of the accessions might have one or two FHB resistance QTLs from Sumai 3. Some accessions with a high level of resistance, may carry different FHB resistance loci or alleles from those in Sumai 3, and are worth further investigation. SSR data also clearly suggested that FHB resistance QTLs on 3BS, 5AS, and 6BS of Sumai 3 were derived from Chinese landrace Taiwan Xiaomai. 相似文献
8.
QTL associated with Fusarium head blight resistance in the soft red winter wheat Ernie 总被引:2,自引:0,他引:2
Liu S Abate ZA Lu H Musket T Davis GL McKendry AL 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2007,115(3):417-427
Fusarium head blight (FHB), mainly caused by Fusarium graminearum Schwabe [telomorph: Gibberella zeae Schw. (Petch)], is an increasingly important disease of wheat (Triticum aestivum L.). Host-plant resistance provides the best hope for reducing economic losses associated with FHB, but new sources of resistance
are limited. The moderately resistant winter wheat cultivar, Ernie, may provide a source of resistance that differs from Sumai
3 but these genes have not been mapped. Also hindering resistance breeding may be associations of resistance with agronomic
traits such as late maturity that may be undesirable in some production environments. This research was conducted to identify
QTL associated with type II FHB resistance (FHB severity, FHBS), and to determine if they are associated with days to anthesis
(DTA), number of spikelets (NOS), and the presence/absence of awns. Two hundred and forty-three F8 recombinant inbred lines from a cross between the resistant cultivar, Ernie and susceptible parent, MO 94-317 were phenotyped
for type II FHB resistance using point inoculation in the greenhouse during 2002 and 2003. Genetic linkage maps were constructed
using 94 simple sequence repeat (SSR) and 146 amplified fragment length polymorphic (AFLP) markers. Over years four QTL regions
on chromosomes 2B, 3B, 4BL and 5A were consistently associated with FHB resistance. These QTL explained 43.3% of the phenotypic
variation in FHBS. Major QTL conditioning DTA and NOS were identified on chromosome 2D. Neither the QTL associated with DTA
and NOS nor the presence/absence of awns were associated with FHB resistance in Ernie. Our results suggest that the FHB resistance
in Ernie appears to differ from that in Sumai 3, thus pyramiding the QTL in Ernie with those from Sumai 3 could result in
enhanced levels of FHB resistance in wheat. 相似文献
9.
10.
C. A. McCartney D. J. Somers G. Fedak R. M. DePauw J. Thomas S. L. Fox D. G. Humphreys O. Lukow M. E. Savard B. D. McCallum J. Gilbert W. Cao 《Molecular breeding : new strategies in plant improvement》2007,20(3):209-221
FHB resistance QTL alleles from Nyuubai, Sumai-3, and Wuhan-1 were evaluated for their effect on Fusarium head blight (FHB)
index, Fusarium damaged kernels (FDK), deoxynivalenol (DON) accumulation, plant height, anthesis date, and numerous grain
quality traits in three elite Canadian spring wheat backgrounds. The three FHB resistance parameters were negatively correlated
with plant height in the three populations. The Wuhan-1 4B resistance allele was the most effective resistance allele but
was associated with a 9.3 cm increase in plant height. The Wuhan-1 2D, Nyuubai 3BSc, Sumai-3 3BSc, Nyuubai 5AS, and Sumai-3
5AS alleles were also effective FHB resistance alleles in these populations. The Nyuubai and Sumai-3 3BS alleles were the
least effective of the FHB resistance alleles in the FHB nursery tests. The Sumai-3 5AS resistance allele was significantly
associated with reduced grain protein content, while the same trend was observed for the Nyuubai 5AS resistance allele but
was not significant. FHB resistance tended to increase with more FHB resistance alleles introgressed into the elite genetic
background, which suggested that marker-assisted selection (MAS) will prove useful for improving FHB resistance in Canadian
germplasm. 相似文献
11.
Maria Buerstmayr Karin Huber Johannes Heckmann Barbara Steiner James C. Nelson Hermann Buerstmayr 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2012,125(8):1751-1765
Breeding for resistance to Fusarium head blight (FHB) in durum wheat continues to be hindered by the lack of effective resistance sources. Only limited information is available on resistance QTL for FHB in tetraploid wheat. In this study, resistance to FHB of a Triticum dicoccum line in the background of three Austrian T. durum cultivars was genetically characterized. Three populations of BC1F4-derived RILs were developed from crosses between the resistant donor line T. dicoccum-161 and the Austrian T. durum recipient varieties DS-131621, Floradur and Helidur. About 130 BC1F4-derived lines per population were evaluated for FHB response using artificial spray inoculation in four field experiments during two seasons. Lines were genetically fingerprinted using SSR and AFLP markers. Genomic regions on chromosomes 3B, 4B, 6A, 6B and 7B were significantly associated with FHB severity. FHB resistance QTL on 6B and 7B were identified in two populations and a resistance QTL on 4B appeared in three populations. The alleles that enhanced FHB resistance were derived from the T. dicoccum parent, except for the QTL on chromosome 3B. All QTL except the QTL on 6A mapped to genomic regions where QTL for FHB have previously been reported in hexaploid wheat. QTL on 3B and 6B coincided with Fhb1 and Fhb2, respectively. This implies that tetraploid and hexaploid wheat share common genomic regions associated with FHB resistance. QTL for FHB resistance on 4B co-located with a major QTL for plant height and mapped at the position of the Rht-B1 gene, while QTL on 7B overlapped with QTL for flowering time. 相似文献
12.
McCartney CA Somers DJ Fedak G Cao W 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2004,109(2):261-271
Fusarium head blight (FHB) reduces grain yield and quality in common and durum wheat. Host FHB resistance is an effective control measure that is achieved by stacking multiple resistance genes into a wheat line. Therefore, breeders would benefit from knowing which resistance sources carry different resistance genes. A diverse collection of FHB-resistant and -susceptible wheat lines was characterized with microsatellite markers linked to FHB resistance quantitative trait loci (QTLs) on chromosomes 2DL, 3BS (distal to the centromere), 3BSc (proximal to the centromere), 4B, 5AS and 6BS identified in wheat lines Maringa, Sumai 3 and Wuhan 1. Putative Sumai 3 QTLs were commonly observed in advanced breeding lines, whereas putative Maringa and Wuhan 1 QTLs were relatively rare. Marker data suggested the 3BS, 3BSc and 5AS QTLs in the Brazilian cv. Maringa were derived from Asian germplasm and not from Frontana or other Brazilian lines. Haplotype diversity was reduced near the 5AS QTL, which might impact the deployment of this QTL. Finally, Brazilian germplasm was not closely related to other resistance sources and might be useful for pyramiding with Asian wheat-derived FHB resistance.Communicated by J. W. Snape 相似文献
13.
Hirokazu Handa Nobukazu Namiki Donghe Xu Tomohiro Ban 《Molecular breeding : new strategies in plant improvement》2008,22(1):71-84
Colinearity in gene content and order between rice and closely related cereal crops has been a powerful tool for gene identification.
Using a comparative genomic approach, we have identified the rice genomic region syntenous to the region of the short arm
of wheat chromosome 2D, on which quantitative trait loci (QTLs) for Fusarium head blight (FHB) resistance and for controlling accumulation of the mycotoxin deoxynivalenol (DON) are closely located.
Utilizing markers known to reside near the FHB resistance QTL and data from several wheat genetic maps, we have limited the
syntenous region to 6.8 Mb of the short arm of rice chromosome 4. From the 6.8-Mb sequence of rice chromosome 4, we found
three putative rice genes that could have a role in detoxification of mycotoxins. DNA sequences of these putative rice genes
were used in BLAST searches to identify wheat expressed sequence tags (ESTs) exhibiting significant similarity. Combined data
from expression analysis and gene mapping of wheat homologues and results of analysis of DON accumulation using doubled haploid
populations revealed that a putative gene for multidrug resistance-associated protein (MRP) is a possible candidate for the
FHB resistance and/or DON accumulation controlling QTLs on wheat chromosome 2DS and can be used as a molecular marker to eliminate
the susceptible allele when the Chinese wheat variety Sumai 3 is used as a resistance source.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users. 相似文献
14.
Martin Löffler Chris-Carolin Schön Thomas Miedaner 《Molecular breeding : new strategies in plant improvement》2009,23(3):473-488
Fusarium head blight (FHB) in wheat results in reduced yield and quality and in accumulation of mycotoxins. The objective of this
study was to identify genomic regions in wheat involved in the control of FHB resistance applying a QTL meta-analysis approach
by combining QTL of 30 mapping populations to propose independent meta-QTL (MQTL). A consensus map was created on which initial
QTL were projected. Nineteen MQTL comprising 2–13 initial QTL with widely varying confidence intervals were found on 12 chromosomes.
Some of them coincided with genomic regions previously identified (e.g. chromosomes 3BS, 6B), however, some MQTL were newly
detected by this study. Separate analysis of populations with the same resistant parent showed a rather high consistency for
the Chinese spring wheat donor ‘Sumai 3’, but little consistency for the Chinese donor ‘Wangshuibai’ and the Swiss donor ‘Arina’.
According to our results breeders can in future (1) choose parents for crossing not comprising the same resistance loci or
QTL intervals, (2) exploit new MQTL, and (3) select markers of some of these MQTL to be used in marker-assisted selection.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users. 相似文献
15.
Xinyao He Helge Skinnes Rebekah E. Oliver Eric W. Jackson Åsmund Bjørnstad 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2013,126(10):2655-2670
Mycotoxins caused by Fusarium spp. is a major concern on food and feed safety in oats, although Fusarium head blight (FHB) is often less apparent than in other small grain cereals. Breeding resistant cultivars is an economic and environment-friendly way to reduce toxin content, either by the identification of resistance QTL or phenotypic evaluation. Both are little explored in oats. A recombinant-inbred line population, Hurdal × Z595-7 (HZ595, with 184 lines), was used for QTL mapping and was phenotyped for 3 years. Spawn inoculation was applied and deoxynivalenol (DON) content, FHB severity, days to heading and maturity (DH and DM), and plant height (PH) were measured. The population was genotyped with DArTs, AFLPs, SSRs and selected SNPs, and a linkage map of 1,132 cM was constructed, covering all 21 oat chromosomes. A QTL for DON on chromosome 17A/7C, tentatively designated as Qdon.umb-17A/7C, was detected in all experiments using composite interval mapping, with phenotypic effects of 12.2–26.6 %. In addition, QTL for DON were also found on chromosomes 5C, 9D, 13A, 14D and unknown_3, while a QTL for FHB was found on 11A. Several of the DON/FHB QTL coincided with those for DH, DM and/or PH. A half-sib population of HZ595, Hurdal × Z615-4 (HZ615, with 91 lines), was phenotyped in 2011 for validation of QTL found in HZ595, and Qdon.umb-17A/7C was again localized with a phenotypic effect of 12.4 %. Three SNPs closely linked to Qdon.umb-17A/7C were identified in both populations, and one each for QTL on 5C, 11A and 13A were identified in HZ595. These SNPs, together with those yet to be identified, could be useful in marker-assisted selection to pyramiding resistance QTL. 相似文献
16.
Zhang G Mergoum M 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2007,115(6):757-766
Kernel shattering (KS) can cause severe grain yield loss in wheat (Triticum aestivum L.). The introduction of genotypes with Fusarium head blight (FHB) resistance has elevated the KS importance. ‘Sumai3,’ the
most commonly used FHB-resistant germplasm worldwide, is reported to be KS susceptible. The objectives of this study were
to detect quantitative trait loci (QTLs) for KS and to determine the relationship between KS and FHB. A recombinant inbred
line population derived from a cross between Sumai3 and ‘Stoa’ was evaluated for KS in five environments and FHB in two field
trials, separately. Four genomic regions on chromosomes 2B, 3B, and 7A were associated with KS. Of them, two major KS QTLs
were detected consistently over three environments and each located proximal to the centromere on chromosomes 3B and 7A. The
resistant alleles at these two QTLs together can reduce KS by 66.1% relative to the reciprocal alleles and by 41.1% compared
to the population mean. The field FHB data revealed four QTLs on chromosomes 2B, 3B, and 7A. Three of these FHB QTLs coincided
with and/or linked to the KS QTLs with opposite allele effects in the corresponding genomic regions, which may explain the
negative correlation (r = −0.29 and P < 0.01) between the KS and FHB infection found in this study. The results in this study indicate that KS and FHB in Sumai3
are, in part, inherited dependently. However, the correlation between KS and FHB is not strong, and the major FHB resistance
QTL on chromosome arm 3BS was not linked to any KS QTL. Our results showed that pyramiding of the two major KS-resistant alleles
and the unlinked major FHB-resistant allele could produce lines with both low values of KS and FHB infection. 相似文献
17.
J. A. Anderson R. W. Stack S. Liu B. L. Waldron A. D. Fjeld C. Coyne B. Moreno-Sevilla J. Mitchell Fetch Q. J. Song P. B. Cregan R. C. Frohberg 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2001,102(8):1164-1168
Genetic resistance to Fusarium head blight (FHB), caused by Fusarium graminearum, is necessary to reduce the wheat grain yield and quality losses caused by this disease. Development of resistant cultivars
has been slowed by poorly adapted and incomplete resistance sources and confounding environmental effects that make screening
of germplasm difficult. DNA markers for FHB resistance QTLs have been identified and may be used to speed the introgression
of resistance genes into adapted germplasm. This study was conducted to identify and map additional DNA markers linked to
genes controlling FHB resistance in two spring wheat recombinant inbred populations, both segregating for genes from the widely
used resistance source ’Sumai 3’. The first population was from the cross of Sumai 3/Stoa in which we previously identified
five resistance QTLs. The second population was from the cross of ND2603 (Sumai 3/Wheaton) (resistant)/ Butte 86 (moderately
susceptible). Both populations were evaluated for reaction to inoculation with F. graminearum in two greenhouse experiments. A combination of 521 RFLP, AFLP, and SSR markers were mapped in the Sumai 3/Stoa population
and all DNA markers associated with resistance were screened on the ND2603/Butte 86 population. Two new QTL on chromosomes
3AL and 6AS wer found in the ND2603/Butte 86 population, and AFLP and SSR markers were identified that explained a greater
portion of the phenotypic variation compared to the previous RFLP markers. Both of the Sumai 3-derived QTL regions (on chromosomes
3BS, and 6BS) from the Sumai 3/Stoa population were associated with FHB resistance in the ND2603/Butte 86 population. Markers
in the 3BS QTL region (Qfhs.ndsu-3BS) alone explain 41.6 and 24.8% of the resistance to FHB in the Sumai 3/Stoa and ND2603/Butte 86 populations, respectively.
This region contains a major QTL for resistance to FHB and should be useful in marker-assisted selection.
Received: 17 August 2000 / Accepted: 16 October 2000 相似文献
18.
A novel quantitative trait locus for Fusarium head blight resistance in chromosome 7A of wheat 总被引:1,自引:0,他引:1
Jayatilake DV Bai GH Dong YH 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2011,122(6):1189-1198
A Chinese Spring-Sumai 3 chromosome 7A disomic substitution line (CS-Sumai 3-7ADSL) was reported to have a high level of Fusarium
head blight (FHB) resistance for symptom spread within a spike (Type II) and low deoxynivalenol accumulation in infected kernels
(Type III), but a quantitative trait locus (QTL) on chromosome 7A has never been identified from this source. To characterize
QTL on chromosome 7A, we developed 191 7A chromosome recombinant inbred lines (7ACRIL) from a cross between Chinese Spring
and CS-Sumai 3-7ADSL and evaluated both types of resistance in three greenhouse experiments. Two major QTL with Sumai 3 origin,
conditioning both Type II and III resistance, were mapped in the short arm of chromosomes 3B (3BS) and near the centromere
of chromosome 7A (7AC). The 3BS QTL corresponds to previously reported Fhb1 from Sumai 3, whereas 7AC QTL, designated as Fhb7AC, is a novel QTL identified from CS-Sumai 3-7ADSL in this study. Fhb7AC explains 22% phenotypic variation for Type II and 24% for Type III resistance. Marker Xwmc17 is the closest marker to Fhb7AC for both types of resistance. Fhb1 and Fhb7AC were additive, and together explained 56% variation for Type II and 41% for Type III resistance and resulted in 66% reduction
in FHB severity and 84% reduction in deoxynivalenol (DON) content. Haplotype analysis of Sumai 3 parents revealed that Fhb7AC originated from Funo, an Italian cultivar. Fhb7AC has the potential to be used in improving wheat cultivars for both types of resistance. 相似文献
19.
Qiongxian Lu Morten Lillemo Helge Skinnes Xinyao He Jianrong Shi Fang Ji Yanhong Dong Åsmund Bjørnstad 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2013,126(2):317-334
Fusarium head blight (FHB) is a destructive wheat disease of global importance. Resistance breeding depends heavily on the Fhb1 gene. The CIMMYT line Shanghai-3/Catbird (SHA3/CBRD) is a promising source without this gene. A recombinant inbred line (RIL) population from the cross of SHA3/CBRD with the German spring wheat cv. Naxos was evaluated for FHB resistance and related traits in field trials using spray and spawn inoculation in Norway and point inoculation in China. After spray and spawn inoculation, FHB severities were negatively correlated with both anther extrusion (AE) and plant height (PH). The QTL analysis showed that the Rht-B1b dwarfing allele co-localized with a QTL for low AE and increased susceptibility after spawn and spray inoculation. In general, SHA3/CBRD contributed most of the favorable alleles for resistance to severity after spray and spawn inoculation, while Naxos contributed more favorable alleles for reduction in FDK and DON content and resistance to severity after point inoculation. SHA3/CBRD contributed a major resistance QTL close to the centromere on 2DLc affecting FHB severity and DON after all inoculation methods. This QTL was also associated with AE and PH, with high AE and tall alleles contributed by SHA3/CBRD. Several QTL for AE and PH were detected, and low AE or reduced PH was always associated with increased susceptibility after spawn and spray inoculation. Most of the other minor FHB resistance QTL from SHA3/CBRD were associated with AE or PH, while the QTL from Naxos were mostly not. After point inoculation, no other QTL for FHB traits was associated with AE or PH, except the 2DLc QTL which was common across all inoculation methods. Marker-assisted selection based on the 2DLc QTL from SHA3/CBRD combined with phenotypic selection for AE is recommended for resistance breeding based on this valuable source of resistance. 相似文献
20.
Molecular mapping of QTL for Fusarium head blight resistance introgressed into durum wheat 总被引:1,自引:0,他引:1
Mingxia Zhao Yueqiang Leng Shiaoman Chao Steven S. Xu Shaobin Zhong 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2018,131(9):1939-1951