哮喘和慢性阻塞性肺疾病患者呼吸阻抗与通气功能相关性分析

目的:明确哮喘和慢性阻塞性肺疾病(COPD)患者呼吸阻抗与通气功能的相关性,以指导诊治疾病。方法:50例哮喘患者纳为A组、50例COPD患者纳为B组、50例健康体检者纳为C组,分别测定其呼吸阻抗指标:呼吸总阻抗(Zrs),5Hz、20Hz时气道阻力(R5、R20)及其差值(R5-R20),5Hz时电抗(X5),中心阻力(Rc),周边阻力(Rp)、共振频率(Fres);通气功能指标:1s用力呼气容积(FEV1)、FEV1占预计值百分比(FEV1%)、FEV1与用力肺活量比值(FEV1/FVC)。分析呼吸阻抗与通气功能的相关性。结果:与C组相比:A组R20明显升高;B组Zrs及R5明显升高;A组与B组的R5-R20、Rc、Rp、Fres明显升高,X5明显降低,但B组数据变化更明显,上述差异均有统计学意义(P0.05)。A组FEV1/FVC及FEV1%与R20均呈负相关,FEV1%与Fres呈负相关;B组FEV1/FVC及FEV1%与Fres呈负相关,上述相关性均有统计学意义(P0.05)。结论:哮喘和COPD均可导致气道阻力增加,但哮喘以增加中心气道阻力为主,COPD以增加外周气道阻力为主;呼吸阻抗能够有效反映通气功能的变化,这对诊治疾病有积极意义。     

脉冲震荡肺功能在稳定期COPD治疗中的应用价值

目的:探讨脉冲震荡肺功能(Impulse oscillometry,IOS)在稳定期COPD患者中应用价值及其和常规肺功能检测指标的相关性。方法:62例重度稳定期COPD患者,同时选择健康对照组人群40例纳入研究。经噻托溴铵联合沙美特罗替卡松治疗,将重度COPD患者缓解至中度。检测治疗前后常规肺功能指标(FEV1/FVC、FEV1)和IOS指标(ZRS、Fres、R5、X5、R20),分析常规肺功能指标和IOS各指标的相关性。结果:COPD患者FEV1和FEV1/FVC和对照组相比明显降低,差异有统计学意义(P0.05),观察组治疗后FEV1和FEV1/FVC明显改善,差异有统计学意义(P0.05)。观察组ZRS、Fres、R5、R20各项指标明显高于对照组,差异有统计学意义(P0.05);治疗后ZRS、Fres、R5明显改善,差异有统计学意义(P0.05)。FEV1、FEV1/FVC和ZRS、Fres、R5呈负相关性(P0.05),和X5呈正相关性(P0.05)。结论:脉冲震荡肺功能多项指标和传统肺功能指标有良好的相关性,是一种简便、低配合度、准确的的肺功能新的检测技术手段。     

人的等呼吸阻力感曲线及相应呼吸型式变化

以13名健康男性为对象,测定了以第2、3、4IND感觉单位平均吸气阻力负荷为参照刺激,属于“等阻力感”的各联合阻力(CR)的总阻力值(R总)、吸气阻力(IR)/呼气阻力(ER)比值;以及呼吸型式参数,如吸气及呼气流率峰值(Imax及Emax),和口腔压力等。结果表明:由物理刺激量R或生理刺激量,如口腔压力峰值(Pmax)、积分均值()、压力积分值(·T),外呼吸功(W)标出的等呼吸阻力感曲线的形状相似,均可由二次多项式拟合。由Pmax标出的等感曲线可见:在单相ER负荷下,引起“等感”的P_(Emax)均有低于相应P_1 msx的趋势;而在CR,则“等感”的口腔压力波动幅度(=|P Imax| PEmax)均较所参照的相应生理刺激量为高。就呼吸不适感的程度而言,属于“等阻力感”关系的各R负荷中,IR>ER>CR。Imax、Emax相对变化取决于JND梯级数及IR/ER比值,而Imax/Emax则只与IR/ER一项因素有关。本文还讨论了CR条件下可耐受更高的机理及其实际意义。     

呼吸阻力感觉的辨别阈梯级——一种辨别阈量表

以9名健康男性为对象,测量了其对于10～500mmH_2O·l~(-1)·S范围吸气(I)或呼气(E)阻力负荷(R)的辨别阈梯级(或“最小可觉差”梯级,JND梯级),以及各梯级所对应的呼吸感觉类别量表值和相应的呼吸型式变化。结果如下:在所观察的阻力范围内,IR或ER负荷的感觉连续体,一般包含6～7个JND梯级。物理或生理刺激量(Y)(如R、口腔压力、外呼吸功等)均与JND感觉单位(X)呈下列指数函数关系:Y=~(A bx)-K(A、b、K—参数)。由多级估量法评量之呼吸感觉量与JND感觉单位亦呈指数函数关系,并能反映I、E在感觉强度上的差别。当感觉强度达3JND时,多数呼吸型式参数已有较明显变化;4JND时,肺通气量仍可维持对照水平。3JND之阻力感觉类别仍属“轻度”,在IR或ER负荷下,其相应的Pmax分别为70或55mmH_2O。本文提出可将JND梯级测量作为一种辨别阈量表用于呼吸感觉评量,并就其对制订呼吸防护装备生理标准的意义进行了讨论。     

实验恒河猴超声心动图的初步研究

分别采用二维、脉冲多普勒及M型超声心动图Teichholtz公式对1 0只健康成年实验恒河猴(Macacamulatta)的心脏结构、心脏血流动力学及左室收缩功能进行测定,并比较不同性别间各参数的差异,以建立正常健康成年恒河猴超声心动图相关生理学参数的基础正常值。结果表明,健康成年实验恒河猴超声心动图各项生理指标类似于人类新生儿的指标,雌、雄组间比较除室间隔舒张末期厚度有统计学差异外(P <0 0 5 ) ,其余指标均无统计学差异(P >0 0 5 )。     

在大气干旱条件下胀果甘草气孔振荡的RLC电路模拟

建立了植物水分输导和蒸腾的电阻(R)、电感(L)、电容(C)电路模型。实验测定出的胀果甘草气孔振荡发生、持续和衰减的阈值与模型分析结果一致。当蒸腾拉力(F)大于输导阻力(R)时气孔开始振荡并使振幅逐渐加大;当F=R时气孔振荡的振幅和频率不变;当0<(R-F)<2(1/2)/(L/C)时气孔振荡开始减弱;当(R-F)>2(1/2)/(L/C)时气孔不会出现振荡。在本实验条件下胀果甘草的R=3.1×10~9MPa·m~(-3)·s,2(1/2)/(L/C)=3.11-3.64×10~5MPa·m~(-3)·s。     

轻,中度体力负荷条件下外加吸气阻力的感觉阈限

以10名健康男青年为被试者,分别于静坐及轻、中度(200、600Kg.m.min~(-1))体力活动条件下观察了外加吸气阻力负荷所引起的面罩腔压力(P)及外呼吸功(w)变化;并采用辨别阈梯级(JND)与多级估量量表(MES)相结合的方法测出了不同体力负荷条件下的呼吸阻力感觉阈限。结果表明:(1)面罩腔压力与外呼吸功分别与呼吸感觉量值呈高度相关关系(P<0.01)但又以压力指标相关程度更为密切,故选用面罩腔压力波动幅度()作为反映呼吸感觉程度的客观生理指标较外吸气功率更为适宜。(2)体力负荷与外加吸气阻力对面罩腔压力、外呼吸功及感觉量值均具有协同的增强效应。(3)随体力负荷的增强,呼吸阻力感觉的阈限值也相应升高。在轻度体力负荷下能引起轻、中度呼吸阻力感觉的面罩腔压力波动幅度阈限值分别为(60、100mmH_2O);在中度体力负荷下,则分别为80、130mmH_2O。述结果对制订航空供氧装备附加呼吸阻力生理学标准具有实际意义。     

中医辨证治疗对改善缓解期儿童哮喘肺功能的临床疗效分析

目的:研究中医辨证治疗对缓解期哮喘患儿肺功能的改善情况,从而为持续肺功能异常的哮喘缓解期患儿提供更好的治疗手段。方法:收集2019.1.1～2019.12.31在上海儿童医学中心呼吸哮喘门诊就诊的儿童哮喘缓解期患儿共88例,收集其相关临床资料,并根据其是否接受中医辨证治疗分为中医辨证治疗组和非中医辨证治疗组,比较其治疗3月后的相关肺功能参数。结果:两组在性别、年龄、病程时间及规范抗哮喘治疗时间之间对比无统计学差异(P0.05)。两组患儿均按照儿童肺功能系列指南肺容积和通气功能部分的肺功能检查流程分别在其入组时及随访3月后进行常规肺通气功能检查,对其肺功能检查结果进行评价。治疗前,两组FEV1%(实测/预计)%、FEV1/VCmax%(实测/预计)%、FEF50%(实测/预计)%、MMEF%(实测/预计)%、FEF75%(实测/预计)%对比差异无统计学意义(P0.05);治疗3个月后,中医辨证治疗组的上述指标均显著升高(P0.05),非中医辨证治疗组的上述指标与治疗前差异无统计学意义(P0.05),组间比较显示中医辨证治疗组的上述指标显著高于非中医辨证治疗组(P0.05)。结论:中医辨证治疗能改善缓解期哮喘患儿的肺功能水平,中医辨证治疗辅以西医规范化抗哮喘治疗能获得更好的临床疗效。     

呼吸训练配合卡介苗多糖核酸雾化吸入对慢阻肺患者康复的研究

目的:用深慢腹式呼吸配合阻力呼吸及吸入氧气的治疗方法,对慢阻肺缓解期患者进行呼吸训练,配合卡介苗多糖核酸注射液雾化吸入,来提高患者呼吸肌的肌力和耐力,防治慢性阻塞性肺疾病(COPD)缓解期患者急性发作,从而改善肺功能、提高生活质量.方法:将120例COPD缓解期患者分为2组,每组60例.其中治疗组进行深慢腹式阻力呼吸锻炼,同时氧气吸入(5L/min),每天2次,每次15分钟进行呼吸训练,加用卡介苗多糖核酸注射液雾化吸入治疗,每次1ml,每周2次,配合常规治疗(包括止咳、祛痰、平喘等);对照组只用常规治疗,两组疗程均为3个月.测定治疗前后肺功能、动脉血气分析、运动耐力、生活质量评估及急性发作次数.结果:治疗组治疗后肺功能、PaO2、SaO2有显著改善,同时运动耐力、生活质量评估亦有提高,而且急性发作次数明显减少;对照组治疗前后肺功能、PaO2、SaO2、运动耐力、生活质量及其急性发作次数均没有明显改善.结论:应用深慢腹式呼吸配合阻力呼吸及吸入氧气进行呼吸训练,配合卡介苗多糖核酸注射液雾化吸入治疗既能改善慢阻肺缓解期患者的肺功能、PaO2、SaO2,提高其运动耐力和生活质量.又能减少慢阻肺缓解期患者急性发作次数.     

呼吸气体流率,压力信号的测量与处理

在呼吸生理研究与临床肺功能检查等工作中,呼吸气体的流率(V)、压力(P)及组分浓度(F)均属最基本的生理变量和测量项目,由此可导出一系列重要呼吸生理参数。记录V(t)信号并处理,即可得到有关呼吸型式、呼吸节律以及肺通气功能等的数据;同时记录并处理V(t)、P(t)信号则可得出呼吸力学诸参数的值;若再结合F(t)进行处理,还可进一步得出有关气体交换、呼吸代谢、心肺功能等的定量资料。目前,     

山西省健康成年人淋巴细胞亚群正常参考值范围

目的:建立山西省健康成人外周血淋巴细胞亚群的正常参考值范围,为机体免疫状态的分析和肿瘤患者的免疫评估提供理论依据。方法:选取山西省1 238例健康成人体检人群,采用流式细胞术测定外周血淋巴细胞亚群的绝对计数和相对计数。结果:确定了健康成人外周血淋巴细胞表达水平,并发现CD3~+T细胞相对计数和绝对计数、CD4~+T细胞相对计数、CD8~+T细胞相对计数和绝对计数、NK细胞相对计数和绝对计数、CD19细胞相对计数和绝对计数、CD4/CD8比值在不同年龄组间存在显著差异性(P0.05);不同性别之间CD8~+T细胞相对计数、CD4~+T细胞绝对计数和CD19细胞绝对计数无统计学意义,CD3~+T细胞、CD8~+T细胞、NK细胞相对计数和绝对计数、CD4~+T细胞、CD19细胞相对计数均存在显著差异(P0.05)。结论:初步建立了山西省健康成年人外周血淋巴细胞亚群参考值范围,为机体免疫功能的评价和肿瘤免疫治疗、诊断提供了参考依据。     

Predicting the Leaf Area and Leaf Dry Weight of Elaeagnus mollis in Shanxi

Non destructive and mathematical approaches of modeling can be very convenient and useful for plant growth estimation. The leaf of Elaeagnus mollis was taken as the object of research. Leaf length、 leaf width、SPAD value and different combinations of these variables were developed models to predict individual leaf area, saturated fresh weight, and dry weight of Elaeagnus mollis. Ten regression equations were compared. Select fitting the best model as a predictive model in leaf area, saturated fresh weight and dry weight. The three models were as follows: individual leaf area LA=3647+0383LW+0001LWS (R=0968), saturated fresh weight SFW=-0464+0081L+000008LWS (R=0963), and dry weight DW=-0094+0032W+00001LS (R=0960). The best prediction model of LA, SFW and DW was validated with the measured value. The results showed that the predicted values and measured values were highly consistent. It could be used to predict the LA, SFW and DW of actual unknown leaves.     

Haematological and biochemical reference values for healthy adults in the middle belt of Ghana

Background

Reference values are very important in clinical management of patients, screening participants for enrolment into clinical trials and for monitoring the onset of adverse events during these trials. The aim of this was to establish gender-specific haematological and biochemical reference values for healthy adults in the central part of Ghana.

Methods

A total of 691 adults between 18 and 59 years resident in the Kintampo North Municipality and South District in the central part of Ghana were randomly selected using the Kintampo Health and Demographic Surveillance System and enrolled in this cross-sectional survey. Out of these, 625 adults made up of 316 males and 309 females were assessed by a clinician to be healthy. Median values and nonparametric 95% reference values for 16 haematology and 22 biochemistry parameters were determined for this population based on the Clinical Laboratory and Standards Institute guidelines. Values established in this study were compared with the Caucasian values being used currently by our laboratory as reference values and also with data from other African and western countries.

Results

Reference values established include: haemoglobin 113–164 g/L for males and 88–144 g/L for females; total white blood cell count 3.4–9.2×10⁹/L; platelet count 88–352×10⁹/L for males and 89–403×10⁹/L for females; alanine aminotransferase 8–54 U/L for males and 6–51 U/L for females; creatinine 56–119 µmol/L for males and 53–106 µmol/L for females. Using the haematological reference values based on the package inserts would have screened out up to 53% of potential trial participants and up to 25% of the population using the biochemical parameters.

Conclusion

We have established a panel of locally relevant reference parameters for commonly used haematological and biochemical tests. This is important as it will help in the interpretation of laboratory results both for clinical management of patients and safety monitoring during a trial.     

兰州地区健康体检者血脂水平分析

目的:通过检测兰州地区健康体检者空腹血脂水平了解本地区人群血脂水平现状及血脂异常情况,建立本地区血脂参考值。方法:采用全自动生化分析仪检测兰州市2328名健康体检者,血清胆固醇(TC)、甘油三酯(TG)、高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C)。比较不同年龄、性别血脂水平差异。结果:本地区2328名被检者,女性TC平均(4.54±0.94)mmol/L,TG中位数1.24mmol/L、HDL-C平均(1.34±0.26)mmol/L、LDL-C平均(2.61±0.76)mmol/L;男性TC平均(4.52±0.84)mmol/L、TG中位数1.56mmol/L mmol/L、HDL-C平均(1.20±0.23)mmol/L LDL-C平均(2.76±0.72)mmol/L,血脂水平随年龄增加逐渐升高(P<0.05)。血脂参考范围为女性TC:2.70~6.38 mmol/L、TG:0.52~3.66 mmol/L、HDL-C:0.83~1.85 mmol/L、LDL-C:1.12~4.10 mmol/L男性:TG:2.87~6.17 mmol/L、0.65~4.00 mmol/L、0.75~1.65 mmol/L、1.35~4.17 mmol/L。男性高TC、高TG、低HDL-C和高LDL-C患病率为18.2%、42.8%、19.6%和28%,女性高TC、高TG、低HDL和高LDL的患病率分别为22.1%、25.5%、2.7%和23.5%。结论:兰州地区血脂水平随年龄、性别、地区不同存在较大差异,临床上不能采用统一标准衡量,而应根据本地区建立的参考值诊断高脂血症。积极控制血脂水平、降低高脂血症患病率预防心脑血管疾病发生。     

Analysis of impulse oscillometric measures of lung function and respiratory system model parameters in small airway-impaired and healthy children over a 2-year period

Background

Is Impulse Oscillometry System (IOS) a valuable tool to measure respiratory system function in Children?Asthma (A) is the most prevalent chronic respiratory disease in children. Therefore, early and accurate assessment of respiratory function is of tremendous clinical interest in diagnosis, monitoring and treatment of respiratory conditions in this subpopulation.IOS has been successfully used to measure lung function in children with a high degree of sensitivity and specificity to small airway impairments (SAI) and asthma. IOS measures of airway function and equivalent electrical circuit models of the human respiratory system have been developed to quantify the severity of these conditions. Previously, we have evaluated several known respiratory models based on the Mead's model and more parsimonious versions based on fitting IOS data known as extended RIC (eRIC) and augmented RIC (aRIC) models have emerged, which offer advantages over earlier models.

Methods

IOS data from twenty-six children were collected and compared during pre-bronchodilation (pre-B) and post- bronchodilation (post-B) conditions over a period of 2 years.

Results and Discussion

Are the IOS and model parameters capable of differentiating between healthy children and children with respiratory system distress?Children were classified into two main categories: Healthy (H) and Small Airway-Impaired (SAI). The IOS measures and respiratory model parameters analyzed differed consistently between H and SAI children. SAI children showed smaller trend of "growth" and larger trend of bronchodilator responses than H children.The two model parameters: peripheral compliance (Cp) and peripheral resistance (Rp) tracked IOS indices of small airway function well. Cp was a more sensitive index than Rp. Both eRIC and aRIC Cps and the IOS Reactance Area, AX, (also known as the "Goldman Triangle") showed good correlations.

Conclusions

What are the most useful IOS and model parameters?In this work we demonstrate that IOS parameters such as resistance at 5 Hz (R5), frequency-dependence of resistance (fdR: R5-R20), reactance area (AX), and parameter estimates of respiratory system such as Cp and Rp provide sensitive indicators of lung function and have the capacity to differentiate between obstructed and non-obstructed airway conditions. They are also capable of demonstrating airway growth-related changes over a two-year period.We conclude that the IOS parameters AX and the eRIC model derived parameter Cp are the most reliable parameters to track lung function in children before and after bronchodilator and over a time period (2 years).Which model is more suitable for interpreting IOS data?IOS data are equally well-modelled by eRIC and aRIC models, based on the close correlations of their corresponding parameters - excluding upper airway shunt compliance. The eRIC model is a more parsimonious and equally powerful model in capturing the differences in IOS indices between SAI and H children. Therefore, it may be considered a clinically-preferred model of lung function.

     

思茅松中幼人工林的生物量碳计量参数

通过开展思茅松中幼人工林样地调查,基于实测数据计算了相关碳计量参数并研究了碳计量参数与相关林分因子的关系.结果表明:1)思茅松中幼人工林生物量转化与扩展因子(BCEF)的平均值为0.5483 Mg·m~(-3)(n=30,95%置信区间:0.5357～0.5609),低于政府间气候变化专门委员会(IPCC)的缺省值.BCEF和平均树高(H)、林分形高(FH)、蓄积量(V)和林龄(A)存在显著负相关(P<0.05).BCEF和平均胸径(D)负相关,但相关不显著(P>0.05).BCEF和N(林分密度)正相关,但相关不显著(P>0.05).BCEF值与林分因子的关系函数拟合效果不佳.2)思茅松中幼人工林生物量扩展因子(BEF)的均值为1.78378(n=30,95%置信区间:1.71714～1.85043),高于IPCC缺省值.BEF和D、H、FH、V和A存在极显著的负相关(P<0.01),与N存在显著的正相关(P<0.05).BEF与A和V的关系以二次曲线函数形式拟合效果较好,与N的关系则以双曲线形式较好,与FH、H和D的关系以幂函数较好.3)思茅松中幼人工林的根茎比(R)均值为0.2400(n=30,95%置信区间:0.2194～0.2606),与IPCC缺省值基本一致.R与D、H、FH、V和A有极显著的负相关关系,与N有显著的正相关关系.R与D、H、FH、V和A的关系以二次曲线的形式拟合效果较好,与N的关系则以双曲线形式拟合较好.     

Association of FcγRIIa R131H polymorphism with idiopathic pulmonary fibrosis severity and progression

Background

Health status, dyspnea and psychological status are important clinical outcomes in chronic obstructive pulmonary disease (COPD). However, forced expiratory volume in one second (FEV₁) measured by spirometry, the standard measurement of airflow limitation, has only a weak relationship with these outcomes in COPD. Recently, in addition to spirometry, impulse oscillometry (IOS) measuring lung resistance (R) and reactance (X) is increasingly being used to assess pulmonary functional impairment.

Methods

We aimed to identify relationships between IOS measurements and patient-reported outcomes in 65 outpatients with stable COPD. We performed pulmonary function testing, IOS, high-resolution computed tomography (CT), and assessment of health status using the St. George's Respiratory Questionnaire (SGRQ), dyspnea using the Medical Research Council (MRC) scale and psychological status using the Hospital Anxiety and Depression Scale (HADS). We then investigated the relationships between these parameters. For the IOS measurements, we used lung resistance at 5 and 20 Hz (R5 and R20, respectively) and reactance at 5 Hz (X5). Because R5 and R20 are regarded as reflecting total and proximal airway resistance, respectively, the fall in resistance from R5 to R20 (R5-R20) was used as a surrogate for the resistance of peripheral airways. X5 was also considered to represent peripheral airway abnormalities.

Results

R5-R20 and X5 were significantly correlated with the SGRQ and the MRC. These correlation coefficients were greater than when using other objective measurements of pulmonary function, R20 on the IOS and CT instead of R5-R20 and X5. Multiple regression analyses showed that R5-R20 or X5 most significantly accounted for the SGRQ and MRC scores.

Conclusions

IOS measurements, especially indices of peripheral airway function, are significantly correlated with health status and dyspnea in patients with COPD. Therefore, in addition to its simplicity and non-invasiveness, IOS may be a useful clinical tool not only for detecting pulmonary functional impairment, but also to some extent at least estimating the patient's quality of daily life and well-being.     

Biomass Carbon Accounting Parameters for Young and Middle Aged Plantation of Simao Pine (Pinus kesiya var.langbianensis)

Based on data collected from field surveys, biomass carbon accounting parameters including biomass conversion and expansion factor (BCEF), biomass expansion factor (BEF) and root shoot ratio (R) for Pinus kesiya var. langbianensis plantation were calculated, and relationships between the parameters and relative stand factors were studied. Main findings were as follows. (1) Mean BCEF for Pinus kesiya var. langbianensis plantation was 05483 Mg m 3(n=30, 95% confidence interval=05357-05609), lower than the IPCC default value. BCEF for Pinus kesiya var langbianensis plantation was negatively related to stand form height (FH), mean stand height (H), stand growing stock (V) and stand age(A) (P<005). BCEF was negatively related to mean diameter at breast height (D), but not statistically significant（P >005）, positively related to stand density (N), not statistically significant（P>005）. Regression equations developed for calculating BCEF with stand factors did not give satisfied estimates. (2) Mean BEF for Pinus kesiya var langbianensis plantation was 178378 (n=30, 95% confidence interval=171714-185043), higher than the IPCC default value. BEF was negatively related to D、H、FH、V and A (P <001), positively related to N (P <005). Regression equations of y=a+bx+cx2 performed well to calculating BEF with A and V as variables. Regression equation of y=a+b/x performed well to calculate BEF with N. Regression equations of y=a xb performed well to calculate BEF with FH、H and D as variables. (3) Mean R for Pinus kesiya varlangbianensis plantation was 02400 (n=30, 95% confidence interval=02194-02606), close to the IPCC default value. R was negatively related to D、H、FH、V and A (P <001), positively related to N (P <005). Regression equations of y= a+bx +cx2 performed well to calculate R with D、H、FH、V and Aas variables. Regression equation of y=a+b/x performed well to calculate R with N.     

人肺炎球菌参考血清09CS中11个血清型抗荚膜多糖的抗体IgG含量的定值

对人肺炎球菌参考血清09CS中11个肺炎球菌血清型(2、8、9N、10A、11A、12F、15B、17F、20、22F、33F)的抗荚膜多糖抗体IgG含量进行定值。方法用WHO推荐的标准检测人血清中抗肺炎球菌荚膜多糖抗体IgG定量ELISA方法,以国际标准血清89SF为标准,对此11个血清型抗荚膜多糖抗体IgG含量进行定值;以暂定的09CS的定值为标准检测12份WHO校正血清、16份兰州生物制品研究所有限责任公司(LIBP)质控血清和89SF,对定值的准确性进一步验证。结果以09CS的定值为标准检测的12份WHO校正血清和16份LIBP质控血清的11个血清型抗荚膜多糖抗体结果,与以89SF为标准检测的结果,均具有良好的直线相关关系(r≈1.00,P0.05);以09CS的定值为标准检测的89SF的11个血清型抗荚膜多糖抗体的新值与其原定值比较,各血清型的误差均20%。结论实验完成了人肺炎球菌参考血清09CS中的11个血清型抗荚膜多糖抗体IgG含量的准确定值。