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1.
Sledge MK Ray IM Jiang G 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2005,111(5):980-992
A genetic map constructed from a population segregating for a trait of interest is required for QTL identification. The goal
of this study was to construct a molecular map of tetraploid alfalfa (Medicago sativa.) using simple sequence repeat (SSR) markers derived primarily from expressed sequence tags (ESTs) and bacterial artificial
chromosome (BAC) inserts of M. truncatula. This map will be used for the identification of drought tolerance QTL in alfalfa. Two first generation backcross populations
were constructed from a cross between a water-use efficient, M. sativa subsp. falcata genotype and a low water-use efficient M. sativa subsp. sativa genotype. The two parents and their F1 were screened with 1680 primer pairs designed to amplify SSRs, and 605 single dose alleles (SDAs) were amplified. In the
F1, 351 SDAs from 256 loci were mapped to 41 linkage groups. SDAs not inherited by the F1, but transmitted through the recurrent parents and segregating in the backcross populations, were mapped to 43 linkage groups,
and 44 of these loci were incorporated into the composite maps. Homologous linkage groups were joined to form eight composite
linkage groups representing the eight chromosomes of M. sativa. The composite maps consist of eight composite linkage groups with 243 SDAs from M. truncatula EST sequences, 38 SDAs from M. truncatula BAC clone sequences, and five SDAs from alfalfa genomic SSRs. The total composite map length is 624 cM, with average marker
density per composite linkage group ranging from 1.5 to 4.4 cM, and an overall average density of 2.2 cM. Segregation distortion
was 10%, and distorted loci tended to cluster on individual homologues of several linkage groups.
Electronic Supplementary Material Supplementary material is available for this article at 相似文献
2.
Mapping of simple sequence repeat (SSR) DNA markers in diploid and tetraploid alfalfa 总被引:11,自引:3,他引:8
N. Diwan J. H. Bouton G. Kochert P. B. Cregan 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2000,101(1-2):165-172
Cultivated alfalfa (Medicago sativa) is an autotetraploid. However, all three existing alfalfa genetic maps resulted from crosses of diploid alfalfa. The current
study was undertaken to evaluate the use of Simple Sequence Repeat (SSR) DNA markers for mapping in diploid and tetraploid
alfalfa. Ten SSR markers were incorporated into an existing F2 diploid alfalfa RFLP map and also mapped in an F2 tetraploid population. The tetraploid population had two to four alleles in each of the loci examined. The segregation of
these alleles in the tetraploid mapping population generally was clear and easy to interpret. Because of the complexity of
tetrasomic linkage analysis and a lack of computer software to accommodate it, linkage relationships at the tetraploid level
were determined using a single-dose allele (SDA) analysis, where the presence or absence of each allele was scored independently
of the other alleles at the same locus. The SDA diploid map was also constructed to compare mapping using SDA to the standard
co-dominant method. Linkage groups were generally conserved among the tetraploid and the two diploid linkage maps, except
for segments where severe segregation distortion was present. Segregation distortion, which was present in both tetraploid
and diploid populations, probably resulted from inbreeding depression. The ease of analysis together with the abundance of
SSR loci in the alfalfa genome indicated that SSR markers should be a useful tool for mapping tetraploid alfalfa.
Received: 10 September 1999 / Accepted: 11 November 1999 相似文献
3.
Molecular mapping of a quantitative trait locus for aluminum tolerance in wheat cultivar Atlas 66 总被引:7,自引:0,他引:7
Ma HX Bai GH Carver BF Zhou LL 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2005,112(1):51-57
Genetic improvement of aluminum (Al) tolerance is one of the cost-effective solutions to improve wheat (Triticum aestivum) productivity in acidic soils. The objectives of the present study were to identify quantitative trait loci (QTL) for Al-tolerance and associated PCR-based markers for marker-assisted breeding utilizing cultivar Atlas 66. A population of recombinant inbred lines (RILs) from the cross Atlas 66/Century was screened for Al-tolerance by measuring root-growth rate during Al treatment in hydroponics and root response to hematoxylin stain of Al treatment. After 797 pairs of SSR primers were screened for polymorphisms between the parents, 131 pairs were selected for bulk segregant analysis (BSA). A QTL analysis based on SSR markers revealed one QTL on the distal region of chromosome arm 4DL where a malate transporter gene was mapped. This major QTL accounted for nearly 50% of the phenotypic variation for Al-tolerance. The SSR markers Xgdm125 and Xwmc331 were the flanking markers for the QTL and have the potential to be used for high-throughput, marker-assisted selection in wheat-breeding programs. 相似文献
4.
Background
Alfalfa (Medicago sativa) is a major forage crop. The genetic progress is slow in this legume species because of its autotetraploidy and allogamy. The genetic structure of this species makes the construction of genetic maps difficult. To reach this objective, and to be able to detect QTLs in segregating populations, we used the available codominant microsatellite markers (SSRs), most of them identified in the model legume Medicago truncatula from EST database. A genetic map was constructed with AFLP and SSR markers using specific mapping procedures for autotetraploids. The tetrasomic inheritance was analysed in an alfalfa mapping population. 相似文献5.
QTLs and epistasis for aluminum tolerance in rice (Oryza sativa L.) at different seedling stages 总被引:11,自引:0,他引:11
P. Wu C. Y. Liao B. Hu K. K. Yi W. Z. Jin J. J. Ni C. He 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2000,100(8):1295-1303
To investigate the genetic background for aluminum (Al) tolerance in rice, a recombinant inbred (RI) population, derived from
a cross between an Al-sensitive lowland indica rice variety IR1552 and an Al-tolerant upland japonica rice variety Azucena, was used in culture solution. A molecular linkage map, together with 104 amplified fragment length
polymorphism (AFLP) markers and 103 restriction fragment length polymorphism (RFLP) markers, was constructed to map quantitative
trait loci (QTLs) and epistatic loci for Al tolerance based on the segregation for relative root length (RRL) in the population.
RRL was measured after stress for 2 and 4 weeks at a concentration of 1mM of Al3+ and a control with a pH 4.0, respectively. Two QTLs were detected at both the 2nd and the 4th weeks on chromosomes 1 and
12 from unconditional mapping, while the QTL on chromosome 1 was only detected at the 2nd stress week from conditional mapping.
The effect of the QTL on chromosome 12 was increased with an increase of the stress period from 2 to 4 weeks. The QTL on chromosome
1 was expressed only at the earlier stress, but its contribution to tolerance was prolonged during growth. At least one different
QTL was detected at the different stress periods. Mean comparisons between marker genotypic classes indicated that the positive
alleles at the QTLs were from the Al-tolerant upland rice Azucena. An important heterozygous non-allelic interaction on Al
tolerance was found. The results indicated that tolerance in the younger seedlings was predominantly controlled by an additive
effect, while an epistatic effect was more important to the tolerance in older seedlings; additionally the detected QTLs may
be multiple allelic loci for Al tolerance and phosphorus-uptake efficiency, or for Al and Fe2+ tolerance.
Received: 29 July 1999 / Accepted: 13 October 1999 相似文献
6.
Li X Wang X Wei Y Brummer EC 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2011,123(4):667-679
Segregation distortion (SD) is often observed in plant populations; its presence can affect mapping and breeding applications.
To investigate the prevalence of SD in diploid alfalfa (Medicago sativa L.), we developed two unrelated segregating F1 populations and one F2 population. We genotyped all populations with SSR markers and assessed SD at each locus in each population. The three maps
were syntenic and largely colinear with the Medicago truncatula genome sequence. We found genotypic SD for 24 and 34% of markers in the F1 populations and 68% of markers in the F2 population; distorted markers were identified on every linkage group. The smaller percentage of genotypic SD in the F1 populations could be because they were non-inbred and/or due to non-fully informative markers. For the F2 population, 60 of 90 mapped markers were distorted, and they clustered into eight segregation distortion regions (SDR). Most
SDR identified in the F1 populations were also identified in the F2 population. Genotypic SD was primarily due to zygotic rather than allelic distortion, suggesting zygotic not gametic selection
is the main cause of SD. On the F2 linkage map, distorted markers in all SDR except two showed heterozygote excess. The severe SD in the F2 population likely biased genetic distances among markers and possibly also marker ordering and could affect QTL mapping of
agronomic traits. To reduce the effects of SD and non-fully informative markers, we suggest constructing linkage maps and
conducting QTL mapping in advanced generation populations. 相似文献
7.
In this study, totally 54 selected polymorphic SSR loci of Chinese shrimp (Fenneropenaeus chinensis), in addition with the previous linkage map of AFLP and RAPD markers, were used in consolidated linkage maps that composed
of SSR, AFLP and RAPD markers of female and male construction, respectively. The female linkage map contained 236 segregating
markers, which were linked in 44 linkage groups, and the genome coverage was 63.98%. The male linkage map contained 255 segregating
markers, which were linked in 50 linkage groups, covering 63.40% of F. chinensis genome. There were nine economically important traits and phenotype characters of F. chinensis were involved in QTL mapping using multiple-QTL mapping strategy. Five potential QTLs associated with standard length (q-standardl-01), with cephalothorax length (q-cephal-01), with cephaloghorax width (q-cephaw-01), with the first segment length (q-firsel-01) and with anti-WSSV (q-antiWSSV-01) were detected on female LG1 and male LG44 respectively with LOD > 2.5. The QTL q-firsel-01 was at 73.603 cM of female LG1. Q-antiWSSV-01 was at 0 cM of male LG44. The variance explained of these five QTLs was from 19.7–33.5% and additive value was from −15.9175
to 7.3675. The closest markers to these QTL were all SSR, which suggested SSR marker was superior to AFLP and RAPD in the
QTL mapping. 相似文献
8.
Lei Wang Caixia Fan Yang Liu Yingping Zhang Shoutang Liu Deqiang Sun Han Deng Ying Xu Yongsheng Tian Xiaolin Liao Mingshu Xie Wenlong Li Songlin Chen 《Marine biotechnology (New York, N.Y.)》2014,16(5):513-521
A recent genetic linkage map was employed to detect quantitative trait loci (QTLs) associated with Vibrio anguillarum resistance in Japanese flounder. An F1 family established and challenged with V. anguillarum in 2009 was used for QTL mapping. Of the 221 simple sequence repeat (SSR) markers used to detect polymorphisms in the parents of F1, 170 were confirmed to be polymorphic. The average distance between the markers was 10.6 cM. Equal amounts of genomic DNA from 15 fry that died early and from 15 survivors were pooled separately to constitute susceptible bulk and resistance bulk DNA. Bulked segregant analysis and QTL mapping were combined to detect candidate SSR markers and regions associated with the disease. A genome scan identified four polymorphic SSR markers, two of which were significantly different between susceptible and resistance bulk (P?=?0.008). These two markers were located in linkage group (LG) 7; therefore, all the SSR markers in LG7 were genotyped in all the challenged fry by single marker analysis. Using two different models, 11–17 SSR markers were detected with different levels of significance. To confirm the associations of these markers with the disease, composite interval mapping was employed to genotype all the challenged individuals. One and three QTLs, which explained more than 60 % of the phenotypic variance, were detected by the two models. Two of the QTLs were located at 48.6 cM. The common QTL may therefore be a major candidate region for disease resistance against V. anguillarum infection. 相似文献
9.
Studer B Boller B Herrmann D Bauer E Posselt UK Widmer F Kölliker R 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2006,113(4):661-671
Bacterial wilt caused by Xanthomonas translucens pv. graminis (Xtg) is a major disease of economically important forage crops such as ryegrasses and fescues. Targeted breeding based on seedling inoculation has resulted in cultivars with considerable levels of resistance. However, the mechanisms of inheritance of resistance are poorly understood and further breeding progress is difficult to obtain. This study aimed to assess the relevance of the seedling screening in the glasshouse for adult plant resistance in the field and to investigate genetic control of resistance to bacterial wilt in Italian ryegrass (Lolium multiflorum Lam.). A mapping population consisting of 306 F1 individuals was established and resistance to bacterial wilt was assessed in glasshouse and field experiments. Highly correlated data (r = 0.67–0.77, P < 0.01) between trial locations demonstrated the suitability of glasshouse screens for phenotypic selection. Analysis of quantitative trait loci (QTL) based on a high density genetic linkage map consisting of 368 amplified fragment length polymorphism (AFLP) and simple sequence repeat (SSR) markers revealed a single major QTL on linkage group (LG) 4 explaining 67% of the total phenotypic variance (Vp). In addition, a minor QTL was observed on LG 5. Field experiments confirmed the major QTL on LG 4 to explain 43% (in 2004) to 84% (in 2005) of Vp and also revealed additional minor QTLs on LG 1, LG 4 and LG 6. The identified QTLs and the closely linked markers represent important targets for marker-assisted selection of Italian ryegrass. 相似文献
10.
Development of simple sequence repeat markers and diversity analysis in alfalfa (Medicago sativa L.)
Zan Wang Hongwei Yan Xinnian Fu Xuehui Li Hongwen Gao 《Molecular biology reports》2013,40(4):3291-3298
Efficient and robust molecular markers are essential for molecular breeding in plant. Compared to dominant and bi-allelic markers, multiple alleles of simple sequence repeat (SSR) markers are particularly informative and superior in genetic linkage map and QTL mapping in autotetraploid species like alfalfa. The objective of this study was to enrich SSR markers directly from alfalfa expressed sequence tags (ESTs). A total of 12,371 alfalfa ESTs were retrieved from the National Center for Biotechnology Information. Total 774 SSR-containing ESTs were identified from 716 ESTs. On average, one SSR was found per 7.7 kb of EST sequences. Tri-nucleotide repeats (48.8 %) was the most abundant motif type, followed by di—(26.1 %), tetra—(11.5 %), penta—(9.7 %), and hexanucleotide (3.9 %). One hundred EST–SSR primer pairs were successfully designed and 29 exhibited polymorphism among 28 alfalfa accessions. The allele number per marker ranged from two to 21 with an average of 6.8. The PIC values ranged from 0.195 to 0.896 with an average of 0.608, indicating a high level of polymorphism of the EST–SSR markers. Based on the 29 EST–SSR markers, assessment of genetic diversity was conducted and found that Medicago sativa ssp. sativa was clearly different from the other subspecies. The high transferability of those EST–SSR markers was also found for relative species. 相似文献
11.
Fukino N Ohara T Monforte AJ Sugiyama M Sakata Y Kunihisa M Matsumoto S 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2008,118(1):165-175
Powdery mildew caused by Podosphaera xanthii is an important foliar disease in melon. To find molecular markers for marker-assisted selection, we constructed a genetic
linkage map of melon based on a population of 93 recombinant inbred lines derived from crosses between highly resistant AR
5 and susceptible ‘Earl’s Favourite (Harukei 3)’. The map spans 877 cM and consists of 167 markers, comprising 157 simple
sequence repeats (SSRs), 7 sequence characterized amplified region/cleavage amplified polymorphic sequence markers and 3 phenotypic
markers segregating into 20 linkage groups. Among them, 37 SSRs and 6 other markers were common to previous maps. Quantitative
trait locus (QTL) analysis identified two loci for resistance to powdery mildew. The effects of these QTLs varied depending
on strain and plant stage. The percentage of phenotypic variance explained for resistance to the pxA strain was similar between
QTLs (R
2 = 22–28%). For resistance to pxB strain, the QTL on linkage group (LG) XII was responsible for much more of the variance
(41–46%) than that on LG IIA (12–13%). The QTL on LG IIA was located between two SSR markers. Using an independent population,
we demonstrated the effectiveness of these markers. This is the first report of universal and effective markers linked to
a gene for powdery mildew resistance in melon. 相似文献
12.
QTL Mapping for Frond Length and Width in Laminaria japonica Aresch (Laminarales, Phaeophyta) Using AFLP and SSR Markers 总被引:1,自引:0,他引:1
Fuli Liu Zhanru Shao Haining Zhang Jidong Liu Xiuliang Wang Delin Duan 《Marine biotechnology (New York, N.Y.)》2010,12(4):386-394
In Laminaria japonica Aresch breeding practice, two quantitative traits, frond length (FL) and frond width (FW), are the most important phenotypic
selection index. In order to increase the breeding efficiency by integrating phenotypic selection and marker-assisted selection,
the first set of QTL controlling the two traits were determined in F2 family using amplified fragment length polymorphism (AFLP) and simple sequence repeat (SSR) markers. Two prominent L. japonicas inbred lines, one with “broad and thin blade” characteristics and another with “long and narrow blade” characteristics, were
applied in the hybridization to yield the F2 mapping population with 92 individuals. A total of 287 AFLP markers and 11 SSR markers were used to construct a L. japonica genetic map. The yielded map was consisted of 28 linkage groups (LG) named LG1 to LG28, spanning 1,811.1 cM with an average
interval of 6.7 cM and covering the 82.8% of the estimated genome 2,186.7 cM. While three genome-wide significant QTL were
detected on LG1 (two QTL) and LG4 for “FL,” explaining in total 42.36% of the phenotypic variance, two QTL were identified
on LG3 and LG5 for the trait “FW,” accounting for the total of 36.39% of the phenotypic variance. The gene action of these
QTL was additive and partially dominant. The yielded linkage map and the detected QTL can provide a tool for further genetic
analysis of two traits and be potential for maker-assisted selection in L. japonica breeding. 相似文献
13.
H. Sabouri A. M. Rezai A. Moumeni A. Kavousi M. Katouzi A. Sabouri 《Biologia Plantarum》2009,53(4):657-662
Oryza sativa L. F2 population and F2:3 derived from a cross between salt tolerance cv. Tarommahali and salt sensitive cv. Khazar were used in this study. A linkage map based on F2 population was constructed (74 SSR markers on 192 individuals), which covered a total of 1231.50 cM with an average two locus interval of 19.83 cM. Two QTLs related to Na+/K+ ratio were found on chromosome 3 and 6. qDM-3 and qDM-8 (for dry mass of shoot) are major QTLs with very large effects explained 20.90 and 17.72 % of the total phenotypic variance, respectively. Major locus for DM (qDM-3) was bracketed by RM1022 — RM6283 spread over 13.6 cM on chromosome 3. Major part of the variability for standard tolerance ranking (STR) was explained by the qSTR-6 flanked by RM3727 — RM340 on chromosome 6, which exhibited phenotypic variance of 17.25 % and peak likelihood ratio (LR) of 17.51. The length of this QTL is 8.8 cM and identification of any tightly linked markers in this region will serve as a candidate gene for fine-mapping. qSTR-3 overlapped with qNA-3 and qNAK-3. The qSTR-3 may contain a new major gene for salt stress tolerance at seedling stage in rice. Major QTLs identified in this paper, after fine-mapping, could be used for marker assisted selection. 相似文献
14.
Elmira Ziya Motalebipour Hatice Gozel Mortaza Khodaeiaminjan Salih Kafkas 《Molecular breeding : new strategies in plant improvement》2018,38(11):134
Pistachio is one of the most commercially important nut trees in the world. To characterize the genetic controls of horticultural traits and facilitate marker-assisted breeding in pistachio, we constructed an SSR-based linkage map using an interspecific F1 population derived from a cross between the cultivar “Siirt” (Pistacia vera L.) and the monoecious Pa-18 genotype of Pistacia atlantica Desf. This population was also used for the first QTL analysis in pistachio on leaf and shoot characters. In total, 1312 SSR primers were screened, and 388 loci were successfully integrated into parental linkage maps. The Siirt maternal map contained 306 markers, while the “Pa-18” paternal map included 285 markers along the 15 linkage groups. The Siirt map spanned 1410.4 cM, with an average marker distance of 4.6 cM; the Pa-18 map covered 1362.5 cM with an average marker distance of 4.8 cM. Phenotypic data were collected during the growing seasons of 2015 and 2016 for four traits: leaf length (LL), leaf width (LW), leaf length/leaf width ratio (LWR), number of leaflet pairs (NLL), and young shoot color (YSC). A total of 17 QTLs were identified in the parental maps. Four QTLs for LL and LW were located on LG2 and LG4, while four QTLs for LWR ratio on LG13 and LG14, two QTLs for NLL and two QTLs for YSC were on LG7 and LG9, respectively, with similar positions in both parental maps. The SSR markers, linkage maps, and QTLs reported here will provide a valuable resource for future molecular and genetic studies in pistachio. 相似文献
15.
Development of an RFLP map in diploid alfalfa 总被引:18,自引:3,他引:15
E. Charles Brummer Joseph H. Bouton Gary Kochert 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1993,86(2-3):329-332
Summary We have developed a restriction fragment length polymorphism (RFLP) linkage map in diploid alfalfa (Medicago sativa L.) to be used as a tool in alfalfa improvement programs. An F2 mapping population of 86 individuals was produced from a cross between a plant of the W2xiso population (M. sativa ssp. sativa) and a plant from USDA PI440501 (M. sativa ssp. coerulea). The current map contains 108 cDNA markers covering 467.5 centimorgans. The short length of the map is probably due to low recombination in this cross. Marker order may be maintained in other populations even though the distance between clones may change. About 50% of the mapped loci showed segregation distortion, mostly toward excess heterozygotes. This is circumstantial evidence supporting the maximum heterozygote theory which states that relative vigor is dependent on maximizing the number of loci with multiple alleles. The application of the map to tetraploid populations is discussed. 相似文献
16.
Musial JM Mackie JM Armour DJ Phan HT Ellwood SE Aitken KS Irwin JA 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2007,114(8):1427-1435
In eastern Australia and California, USA, one of the major lethal fungal diseases of lucerne (Medicago sativa) is Stagonospora root and crown rot, caused by Stagonospora meliloti. Quantitative trait loci (QTL) involved in resistance and susceptibility to S. meliloti were identified in an autotetraploid lucerne backcross population of 145 individuals. Using regression analysis and interval
mapping, we detected one region each on linkage groups 2, 6 and 7 that were consistently associated with disease reaction
to S. meliloti in two separate experiments. The largest QTL on linkage group 7, which is associated with resistance to S. meliloti, contributed up to 17% of the phenotypic variation. The QTL located on linkage group 2, which is potentially a resistance
allele in repulsion to the markers for susceptibility to S. meliloti, contributed up to 8% of the phenotypic variation. The QTL located on linkage group 6, which is associated with susceptibility
to S. meliloti, contributed up to 16% of the phenotypic variation. A further two unlinked markers contributed 5 and 8% of the phenotypic
variation, and were detected in only one experiment. A total of 517 simple sequence repeat (SSR) markers from Medicago truncatula were screened on the parents of the mapping population. Only 27 (6%) SSR markers were polymorphic and could be incorporated
into the autotetraploid map of M. sativa. This allowed alignment of our M. sativa linkage map with published M. truncatula maps. The markers linked to the QTL we have reported will be useful for marker assisted selection for partial resistance
to S. meliloti in lucerne. 相似文献
17.
Quantitative trait loci for aluminum resistance in wheat 总被引:4,自引:0,他引:4
Li-Li Zhou Gui-Hua Bai Hong-Xiang Ma Brett F. Carver 《Molecular breeding : new strategies in plant improvement》2007,19(2):153-161
Quantitative trait loci (QTL) for wheat resistance to aluminum (Al) toxicity were analyzed using simple sequence repeats (SSRs)
in a population of 192 F6 recombinant inbred lines (RILs) derived from a cross between an Al-resistant cultivar, Atlas 66 and an Al-sensitive cultivar,
Chisholm. Wheat reaction to Al was measured by relative root growth and root response to hematoxylin stain in nutrient-solution
culture. After screening 1,028 SSR markers for polymorphisms between the parents and bulks, we identified two QTLs for Al
resistance in Atlas 66. One major QTL was mapped on chromosome 4D that co-segregated with the Al-activated malate transporter
gene (ALMT1). Another minor QTL was located on chromosome 3BL. Together, these two QTLs accounted for about 57% of the phenotypic variation
in hematoxylin staining score and 50% of the variation in net root growth (NRG). Expression of the minor QTL on 3BL was suppressed
by the major QTL on 4DL. The two QTLs for Al resistance in Atlas 66 were also verified in an additional RIL population derived
from Atlas 66/Century. Several SSR markers closely linked to the QTLs were identified and have potential to be used for marker-assisted
selection (MAS) to improve Al-resistance of wheat cultivars in breeding programs. 相似文献
18.
Osman A. Gutiérrez Arin F. Robinson Johnie N. Jenkins Jack C. McCarty Martin J. Wubben Franklin E. Callahan Robert L. Nichols 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2011,122(2):271-280
The identification of molecular markers that are closely linked to gene(s) in Gossypium barbadense L. accession GB713 that confer a high level of resistance to reniform nematode (RN), Rotylenchulus reniformis Linford & Oliveira, would be very useful in cotton breeding programs. Our objectives were to determine the inheritance of
RN resistance in the accession GB713, to identify SSR markers linked with RN resistance QTLs, and to map these linked markers
to specific chromosomes. We grew and scored plants for RN reproduction in the P1, P2, F1, F2, BC1P1, and BC1P2 generations from the cross of GB713 × Acala Nem-X. The generation means analysis using the six generations indicated that
one or more genes were involved in the RN resistance of GB713. The interspecific F2 population of 300 plants was genotyped with SSR molecular markers that covered most of the chromosomes of Upland cotton (G. hirsutum L.). Results showed two QTLs on chromosome 21 and one QTL on chromosome 18. One QTL on chromosome 21 was at map position
168.6 (LOD 28.0) flanked by SSR markers, BNL 1551_162 and GH 132_199 at positions 154.2 and 177.3, respectively. A second
QTL on chromosome 21 was at map position 182.7 (LOD 24.6) flanked by SSR markers BNL 4011_155 and BNL 3279_106 at positions
180.6 and 184.5, respectively. Our chromosome 21 map had 61 SSR markers covering 219 cM. One QTL with smaller genetic effects
was localized to chromosome 18 at map position 39.6 (LOD 4.0) and flanked by SSR markers BNL 1721_178 and BNL 569_131 at positions
27.6 and 42.9, respectively. The two QTLs on chromosome 21 had significant additive and dominance effects, which were about
equal for each QTL. The QTL on chromosome 18 showed larger additive than dominance effects. Following the precedent set by
the naming of the G. longicalyx Hutchinson & Lee and G. aridum [(Rose & Standley) Skovsted] sources of resistance, we suggest the usage of Ren
barb1
and Ren
barb2
to designate these QTLs on chromosome 21 and Ren
barb3
on chromosome 18. 相似文献
19.
Irina Klimenko Nadejda Razgulayeva Mitsuru Gau Kenji Okumura Akihiro Nakaya Satoshi Tabata Nicolay N. Kozlov Sachiko Isobe 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2010,120(6):1253-1263
Red clover (Trifolium pratense L.) is a diploid (2n = 14), self-incompatible legume that is widely cultivated as a forage legume in cold geographical regions. Because it is
a short-lived perennial species, improvement of plant persistency is the most important objective for red clover breeding.
To develop a marker-assisted selection (MAS) approach for red clover, we identified candidate QTLs related to plant persistency.
Two full-sib mapping populations, 272 × WF1680 and HR × R130, were used for QTL identification. Resistance to Sclerotinia trifoliorum and Fusarium species, as well as to winter hardiness, was investigated in the laboratory and in field experiments in Moscow region (Russia),
and Sapporo (Japan). With the genotype data derived from microsatellite and other DNA markers, candidate QTLs were identified
by simple interval mapping (SIM), Kruskal–Wallis analysis (KW analysis) and genotype matrix mapping (GMM). A total of 10 and
23 candidate QTL regions for plant persistency were identified in the 272 × WF1680 and the HR × R130 mapping populations,
respectively. The QTLs identified by multiple mapping approaches were mapped on linkage group (LG) 3 and LG6. The significant
QTL interactions identified by GMM explained the higher phenotypic variation than single effect QTLs. Identification of haplotypes
having positive effect QTLs in each parent were first demonstrated in this study for pseudo-testcross mapping populations
in plant species using experimental data. 相似文献
20.
The first SSR-based genetic linkage map for cultivated groundnut (Arachis hypogaea L.) 总被引:1,自引:0,他引:1
R. K. Varshney D. J. Bertioli M. C. Moretzsohn V. Vadez L. Krishnamurthy R. Aruna S. N. Nigam B. J. Moss K. Seetha K. Ravi G. He S. J. Knapp D. A. Hoisington 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2009,118(4):729-739
Molecular markers and genetic linkage maps are pre-requisites for molecular breeding in any crop species. In case of peanut
or groundnut (Arachis hypogaea L.), an amphidiploid (4X) species, not a single genetic map is, however, available based on a mapping population derived
from cultivated genotypes. In order to develop a genetic linkage map for tetraploid cultivated groundnut, a total of 1,145
microsatellite or simple sequence repeat (SSR) markers available in public domain as well as unpublished markers from several
sources were screened on two genotypes, TAG 24 and ICGV 86031 that are parents of a recombinant inbred line mapping population.
As a result, 144 (12.6%) polymorphic markers were identified and these amplified a total of 150 loci. A total of 135 SSR loci
could be mapped into 22 linkage groups (LGs). While six LGs had only two SSR loci, the other LGs contained 3 (LG_AhXV) to
15 (LG_AhVIII) loci. As the mapping population used for developing the genetic map segregates for drought tolerance traits,
phenotyping data obtained for transpiration, transpiration efficiency, specific leaf area and SPAD chlorophyll meter reading
(SCMR) for 2 years were analyzed together with genotyping data. Although, 2–5 QTLs for each trait mentioned above were identified,
the phenotypic variation explained by these QTLs was in the range of 3.5–14.1%. In addition, alignment of two linkage groups
(LGs) (LG_AhIII and LG_AhVI) of the developed genetic map was shown with available genetic maps of AA diploid genome of groundnut
and Lotus and Medicago. The present study reports the construction of the first genetic map for cultivated groundnut and demonstrates its utility
for molecular mapping of QTLs controlling drought tolerance related traits as well as establishing relationships with diploid
AA genome of groundnut and model legume genome species. Therefore, the map should be useful for the community for a variety
of applications.
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