共查询到20条相似文献,搜索用时 109 毫秒
1.
M. Rossi F. Carrari J. L. Cabrera-Ponce C. Vázquez-Rovere L. Herrera-Estrella G. Gudesblat N. D. Iusem 《Molecular genetics and genomics : MGG》1998,258(1-2):1-8
Asr is a family of genes that maps to chromosome 4 of tomato. Asr2, a recently reported member of this family, is believed to be regulated by abscisic acid (ABA), stress and ripening. A genomic Asr2 clone has been fully sequenced, and candidate upstream regulatory elements have been identified. To prove that the promoter region is functional in vivo, we fused it upstream of the β-glucuronidase (GUS) reporter gene. The resulting chimeric gene fusion was used for transient expression assays in papaya embryogenic calli and leaves. In addition, the same construct was used to produce transgenic tomato, papaya, tobacco, and potato plants. Asr2 upstream sequences showed promoter function in all of these systems. Under the experimental conditions tested, ABA stimulated GUS expression in papaya and tobacco, but not in tomato and potato systems. 相似文献
2.
In a study of the 5′-flanking sequence of the Zea mays L. (maize) Glb1 gene in vitro, serial promoter deletions were generated and linked with the β-glucuronidase (GUS) reporter gene. The promoter deletion-GUS fusions were introduced into the maize P3377 cell line by particle
bombardment. GUS assays indicated that treatment of the maize cultured cells with abscisic acid (ABA) was required for Glb1-driven GUS transient expression, and that the –272-bp sequence of the Glb1 promoter was sufficient for ABA-regulated expression of GUS. The longest undeleted sequence used, –1391 GUS, showed relatively
low expression which could be indicative of an upstream silencer element in the Glb1 promoter between –1391 and –805. Further studies show that the Glb1-driven GUS activity of bombarded maize P3377 cells increases with increasing ABA concentration (up to 100–300 μm). Site-directed mutagenesis of a putative ABA response element, Em1a, abolished GUS expression in P3377 cells. This observation
indicated that the Em1a sequence in the Glb1 5′ regulatory region is responsible for the positive ABA regulation of gene expression.
Received: 9 May 1997 / Revision received: 9 November 1997 / Accepted: 8 December 1997 相似文献
3.
M. Rossi D. Lijavetzky H. E. Hopp N. Iusem D. Bernacchi M. Rossi H. E. Hopp N. Iusem 《Molecular & general genetics : MGG》1996,252(4):489-492
Asr1, Asr2 andAsr3 are three homologous clones isolated from tomato whose expression is believed to be regulated by abscisic acid (ABA); the corresponding genes thus participate in physiological and developmental processes such as responses of leaf and root to water stress, and fruit ripening. In this report, results obtained with Near Isogenic Lines reveal thatAsr1, Asr2 andAsr3 represent three different loci. In addition, we map these genes on the restriction fragment length polymorphism (RFLP) map of the tomato genome by using an F2 population derived from an interspecific hybrid crossL. esculentum × L. penelli. RFLP data allow us to map these genes on chromosome 4, suggesting that they belong to a gene family. The elucidation of the genomic organization of theAsr gene family may help in understanding the role of its members in the response to osmotic stress, as well as in fruit ripening, at the molecular level. 相似文献
4.
Functional characterization of a cotton late embryogenesis-abundant D113 gene promoter in transgenic tobacco 总被引:4,自引:0,他引:4
Previous studies have shown that mRNA and protein encoded by late embryogenesis-abundant (LEA) gene D113 from Gossypium
hirsutum L. accumulate at high levels in mature seeds and also in response to abscisic acid (ABA) in young embryo. In this study,
we studied the expression of four promoter 5′ deletion constructs (−1383, −974, −578 and −158) of the LEA D113 gene fused to beta-glucuronidase (GUS). GUS activity analysis revealed that the −578 promoter fragment was necessary to direct
seed-specific GUS expression in transgenic tobacco plants (Nicotiana tabacum L.). To further investigate the expression pattern of LEA D113 promoter under environmental stresses, 2-week-old transgenic tobacco seedlings were exposed to ABA, dehydration, high salinity
and cold treatments. GUS activity in the seedlings was quantified fluorimetrically, and expression was also observed by histochemical
staining. An apparent increase in GUS activity was found in plants harboring constructs −1383, −974 and −578 after 24 h of
ABA or high-salinity treatments, as well as after 10 days of dehydration. By contrast, only a slight increase was observed
in all the three lines after cold treatment. Virtually no change in expression was found in construct −158 in response to
dehydration, salinity and cold, but there was a moderate response to ABA, suggesting that the region between −574 and −158
was necessary for dehydration- and salinity-dependent expression, whereas ABA-responsive cis-acting elements might be located in the −158 region of the promoter. 相似文献
5.
A promoter fusion (Sh35) combining upstream regulatory regions from the maize Sh1 promoter with a truncated 35S promoter, Δ9035 (–90 to +8) has been compared with the original Sh1 promoter for its capacity to promote expression of the β-glucuronidase (GUS) gene in stably transformed tomato plants. For both promoters, very faint GUS expression was detected
in the vegetative tissues, and no expression was detected in the fruit pericarp tissues. However, in the seed, Sh1 promoted low GUS expression but Sh35 directed 25-fold higher GUS expression. For both constructs, the profile of GUS expression
was similar to that of endogenous sucrose synthase activity, but maximal GUS activity was reached 15 days after the peak of
sucrose synthase activity.
Received: 20 October 1998 / Revision received: 1 December 1998 / Accepted: 14 December 1998 相似文献
6.
Chen X Wang Z Gu R Fu J Wang J Zhang Y Wang M Zhang J Jia J Wang G 《Plant cell reports》2007,26(9):1555-1565
By screening a genomic library of maize, a 2.2 kb 5′ flanking fragment of Zpu1 gene, encoding the pullulanase-type starch debranching enzyme, was isolated. Promoter fragments of various lengths, including
the full 5′ flanking sequence (−2267 to −1) (Z1), a 3′ deletion (−2267 to −513) (Z5) and three 5′ deletions extending to −1943
(Z2), −1143 (Z3) and −516 (Z4) upstream of the translational initiation codon (ATG), were fused to the GUS reporter gene and
introduced into tobacco. When these constructs were tested in transgenic tobacco plants, seed-preferred GUS activity was observed
in pZ1-transgenic lines. In pZ2-transgenic lines, the GUS activity was not only restricted to seeds, but was also detected
in calyxes, petals, stamens and mature leaves. At the same time, negligible GUS activity was detected in roots, stems, young
leaves, stigmas and ovaries from the transgenic tobacco plants, which had integrated the full isolated sequence of Zpu1 promoter or its deletions. Deletion analysis indicated that the promoter contained a putative positive cis-regulatory element and the proximal region (−516 to −1) was essential for directing the expression of gus reporter gene. Analysis of GUS activity during the fruit development and seed germination suggested that Zpu1 promoter is active both in starch anabolism and in starch catabolism, which is consistent with the function of the endogenous
gene in maize. GUS activity in leaves under light and darkness confirmed that Zpu1 promoter functions in the starch degradation of photosynthetic tissues in the dark phase of the diurnal cycle. 相似文献
7.
8.
9.
Tolerance to water deficits was evolutionarily relevant to the conquest of land by primitive plants. In this context, epigenetic events may have played important roles in the establishment of drought stress responses. We decided to inspect epigenetic marks in the plant organ that is crucial in the sensing of drought stress: the root. Using tomato as a crop model plant, we detected the methylated epialleles of Asr2, a protein-coding gene widespread in the plant kingdom and thought to alleviate restricted water availability. We found 3 contexts (CG, CNG, and CNN) of methylated cytosines in the regulatory region of Solanum lycopersicum Asr2 but only one context (CG) in the gene body. To test the hypothesis of a link between epigenetics marks and the adaptation of plants to drought, we explored the cytosine methylation status of Asr2 in the root resulting from water-deficit stress conditions. We found that a brief exposure to simulated drought conditions caused the removal of methyl marks in the regulatory region at 77 of the 142 CNN sites. In addition, the study of histone modifications around this model gene in the roots revealed that the distal regulatory region was rich in H3K27me3 but that its abundance did not change as a consequence of stress. Additionally, under normal conditions, both the regulatory and coding regions contained the typically repressive H3K9me2 mark, which was lost after 30 min of water deprivation. As analogously conjectured for the paralogous gene Asr1, rapidly acquired new Asr2 epialleles in somatic cells due to desiccation might be stable enough and heritable through the germ line across generations, thereby efficiently contributing to constitutive, adaptive gene expression during the evolution of desiccation-tolerant populations or species. 相似文献
10.
In order to understand the molecular mechanisms which are responsible for desiccation tolerance in the resurrection plant
Craterostigma plantagineum Hochst. a thorough analysis of the CDeT11-24 gene family was performed. CDeT11-24 comprises a small gene family whose genes
are expressed in response to dehydration, salt stress and abscisic acid (ABA) treatment in leaves. The gene products are constitutively
expressed in roots and disappear only when the plants are transferred to water. It is therefore suggested that the proteins
are involved in sensing water status. The predicted proteins are very hydrophilic; they share some features with late-embryogenesis-abundant
proteins, and sequence similarities were found with two ABA- and drought-regulated Arabidopsis genes. The analysis of β-glucuronidase reporter genes driven by the CDeT11-24 promoter showed high activity in mature seeds
in both transgenic Arabidopsis and tobacco. In vegetative tissues the promoter activity in response to ABA was restricted to young Arabidosis seedlings. The responsiveness to ABA during later developmental stages was regained in the presence of the Arabidopsis gene product ABI3. Dehydration-induced promoter activity was only observed in Arabidopsis leaves at a particular developmental stage. This analysis indicates that some components in the signal transduction pathway
of the resurrection plant are not active in tobacco or Arabidopsis.
Received: 26 April 1997 / Accepted: 16 July 1997 相似文献
11.
Gu R Zhao L Zhang Y Chen X Bao J Zhao J Wang Z Fu J Liu T Wang J Wang G 《Plant cell reports》2006,25(11):1157-1165
The β-glucosidase gene of maize (ZmGLU1) was suggested to hydrolyze cytokinin-conjugate and release free cytokinin during plant growth and development. A clone containing the upstream region of ZmGLU1 was isolated and sequenced from a maize genomic library. The full-length ZmGLU1 promoter and a series of its 5′ deletions were fused to the beta-glucuronidase (GUS) reporter gene and transferred into tobacco. The GUS activity of transgenic plants was assayed at various developmental stages. The results showed that ZmGLU1 promoter-driven GUS gene had the highest expression level in the roots and that the expression of GUS gene declined during seed maturation and down to the lowest level in mature seeds. The ZmGLU1 promoter-driven GUS expression increased during seed germination, reaching a peak on day 11. The results also showed that this promoter could be inhibited by 6-BA, trans-zeatin, and NAA, but was not affected by GA3, ABA, SA, cold, salt, drought, and submergence treatments. The histochemical staining revealed that GUS activity was located in vigorous cell division zones with dominant staining associated with vascular tissues. Deletion analysis showed that the promoter contained a putative leaf-specific and stem-specific negative regulative element and two putative enhancers. 相似文献
12.
M. Rossi D. Lijavetzky H. E. Hopp N. Iusem D. Bernacchi M. Rossi H. E. Hopp N. Iusem 《Molecular genetics and genomics : MGG》1996,252(4):489-492
Asr1, Asr2 andAsr3 are three homologous clones isolated from tomato whose expression is believed to be regulated by abscisic acid (ABA); the corresponding genes thus participate in physiological and developmental processes such as responses of leaf and root to water stress, and fruit ripening. In this report, results obtained with Near Isogenic Lines reveal thatAsr1, Asr2 andAsr3 represent three different loci. In addition, we map these genes on the restriction fragment length polymorphism (RFLP) map of the tomato genome by using an F2 population derived from an interspecific hybrid crossL. esculentum × L. penelli. RFLP data allow us to map these genes on chromosome 4, suggesting that they belong to a gene family. The elucidation of the genomic organization of theAsr gene family may help in understanding the role of its members in the response to osmotic stress, as well as in fruit ripening, at the molecular level. 相似文献
13.
14.
15.
16.
Tania Jacinto Barry McGurl Vincent Franceschi John Delano-Freier Clarence A. Ryan 《Planta》1997,203(4):406-412
Tomato (Lycopersicon esculentum Mill. cv. Better Boy) plants were transformed with a fused gene containing a 2.2-kb promoter fragment of the tomato prosystemin
gene and the coding region of the β-glucuronidase (GUS) reporter gene. The transgenic plants exhibited a low constitutive
level of prosystemin-β-glucuronidase gene expression, assayed by histochemical staining and GUS enzyme activity, that was
associated in the vascular bundles of leaf main veins, petiolules, petioles and stems. The GUS activity in the vascular bundles
in each tissue was increased by wounding and by treatment of the plants with methyl jasmonate, similar to the induction of
prosystemin in wild-type plants. The increase in GUS activity in the vascular bundles of leaves in response to wounding correlated
with the wound-inducible increase in prosystemin mRNA. Tissue printing, using rabbit anti-serum prepared against prosystemin,
confirmed that inducible prosystemin protein was localized in vascular bundles of petiolules, petioles and stems of wild-type
tomato plants. The evidence indicates that the 2.2-kb promoter region of the tomato prosystemin gene contains elements conferring
its correct temporal and spatial expression in the vascular bundles of transgenic tomato plants.
Received: 7 January 1997 / Accepted: 2 April 1997 相似文献
17.
Xiangbin Xu Sai Guo Kai Chen Hongmiao Song Junjun Liu Longbiao Guo Qian Qian Huizhong Wang 《Biotechnology letters》2010,32(10):1533-1539
A 1681 bp PsPR10 promoter was isolated from Pinus strobus and a series of 5′-deletions were fused to the β-glucuronidase (GUS) reporter gene and introduced into tobacco. GUS activity in P796 (−796 to +69) construct transgenic plant roots was similar
with that of P1681 and higher than those of the P513 (−513 to +69) and P323 (−323 to +69) transgenic plants. Moreover, the
abiotic stresses of NaCl, PEG 6000 and mannitol, and salicylic acid (SA), abscisic acid (ABA) and jasmonic acid (JA) induced
higher GUS activity in the roots of P796 transgenic tobacco. This study provides a potential inducible root-specific promoter
for transgenic plants. 相似文献
18.
Saad RB Romdhan WB Zouari N Azaza J Mieulet D Verdeil JL Guiderdoni E Hassairi A 《Transgenic research》2011,20(5):1003-1018
19.
20.
M. Ficker H.-H. Kirch R. Eijlander E. Jacobsen R. D. Thompson 《Molecular & general genetics : MGG》1998,257(2):132-142
A functional analysis of the promoter of the S
2
-RNase gene from potato was performed in transgenic potato and tobacco plants, using a deletion series of S
2
-RNase promoter GUS fusions. A detailed histochemical and quantitative analysis of the transgenic tobacco plants revealed that S
2
promoter fragments ranging in size from 5.6 kb in length down to 0.2 kb mediate a weak developmentally regulated expression
in the pistil, and strong ectopic expression in pollen. In the pistil, different expression patterns were seen depending on
the transformant, the predominant one being characterised by expression in the stigma and the transmitting tract of the style,
whereas a few plants showed expression exclusively either in the stigma or in the stylar transmitting tissue. All transformants
also showed GUS expression in the placental epidermis of the ovary. Two sequences that are conserved between the potato S
1
-RNase and S
2
-RNase promoters, termed motif I and motif III, are located in a fragment of the S
2
promoter extending from position −200 to bp −100, and motif II, located between bp −498 and −480, was identified on the basis
of sequence comparisons between pistil-specific promoters. Motif II was found to be dispensible for pistil-specific and for
pollen-specific expression. Two submotifs, A and B, were identified within motif I. Both were essential for expression in
the pistil but only B was necessary for expression in pollen. Although motif III has a similar bipartite structure and sequence
to motif I, it was not sufficient to confer either pollen- or pistil-specific expression. However, deletion of motif III abolished
pollen-specific expression in transient expression experiments, suggesting that an interaction between the two sequence motifs
may be needed to specify cell type-specific expression. In transgenic potato the S
2
-RNase promoter also mediates expression in pollen and in the pistil; however, significantly fewer plants showed expression than
in tobacco, with most plants also exhibiting GUS expression in other tissues.
Received: 7 August 1997 / Accepted: 8 September 1997 相似文献