超敏C反应蛋白检测对冠心病诊断价值分析

目的:探讨超敏C反应蛋白检测(hs-CRP)在冠心病诊断中的临床应用价值。方法:分别检测52例急性心肌梗死(AMI)、42例不稳定型心绞痛(UAP)、32例稳定型心绞痛(SAP)和48例正常对照者血清hs-CRP含量,比较各组间指标的水平差异。结果:AMI、UAP和SAP组患者血清hs-CRP浓度明显高于正常对照组(p0.05),冠心病患者各组浓度比较,从高到低排序为:AMI组、UAP组、SAP组。结论:hs-CRP水平的增高与冠心病的发生、发展显著相关,是预测和评价冠心病的重要指标,具有重要的临床应用价值。     

血浆及外周血中性粒细胞内髓过氧化物酶水平在冠心病临床诊断中的意义

目的:研究冠心病患者外周循环血中性粒细胞中髓过氧化物酶指数(MPXI)和血浆中髓过氧化物酶(MPO)浓度的改变及两者间相关性,探讨两者在冠心病临床诊断中的意义。方法:随机选取冠心病患者55例,按病情严重程度分为急性冠脉综征(ACS)组35例[包括不稳定心绞痛(UAP)组21例和急性心肌梗死(AMI)组14例]和稳定心绞痛(SAP)组20例,同时随机选取健康体检人员60例为正常对照组;采用ADVIA2120全自动血细胞分析仪检测MPXI,取血浆用ELISA法检测血浆MPO浓度。结果:ACS组MPXI(6.31±4.24)显著高于SAP组(3.38±2.14)和对照组(2.78±2.12),差异有统计学意义(P<0.05);SAP组MPXI高于正常对照组,差异无统计学意义(P>0.05)。ACS组血浆中MPO(1204.07±838.61 pmol/L)显著高于SAP组(755.97±426.23 pmol/L)和对照组(290.45±99.87 pmol/L),差异有统计学意义(P<0.05);SAP组MPO显著高于正常对照组,差异有统计学意义(P<0.05)。SAP组MPXI与MPO无相关性(r=0.424,P>0.05),ACS组MPXI与MPO呈负相关(r=-0.536,P<0.05)。结论:MPXI和MPO在冠心病的诊断中有意义,MPXI与血浆中MPO在ACS组中呈负相关。     

冠心病患者血清sCD40L水平及其与hs-CRP关系研究

目的:研究冠心病患者血清sCD40L和hs-CRP临床特点及其关系,探讨其在冠心病预测和治疗中的意义.方法:采用夹心法酶联免疫吸附测定分析法及微粒子增强透射免疫分析法分别对对照组15例、稳定型心绞痛(SAP)组27例、不稳定型心绞痛(UAP)组35例及急性心肌梗死(AMI)组14例受试者血清sCD40L和hs-CRP水平进行检测,并观察其与冠脉狭窄程度的相关性.结果:1)、血清hs-CRP水平:SAP组、UAP组、AMI组呈依次递增(AMI组比UAP组、UAP组比SAP组P＜0.05,AMI组、UAP组比对照组(P＜0.01);SAP组与对照组血清hs-CRP水平相似;2)、血清sCIMOL水平:AMI组及UAP组血清sCD40L水平高于SAP组和对照组(P＜0.01),AMI组与UAP组之间、SAP与对照组间没有统计学差异;3)、相关分析显示血清sCD40L水平与hs-CRP水平显著相关(r=0.787,P＜0.0001),两者均与冠脉狭窄程度无相关性.结论:血清hs-CRP、sCD40L水平升高与冠心病临床表现类型和病情是否稳定有关,与冠状动脉狭窄程度无关,此两项指标能够用于冠心病病情不稳定性的判断和预测.     

脑钠肽、和肽素检测对冠脉病变程度的预测价值研究

目的:探讨冠心病急者脑钠肽、和肽素与冠脉病变程度之间的关系.方法:入选急性心肌梗死患者(AMI组)40例、不稳定型心绞痛患者(UAP)36例、稳定型心绞痛患者(SAP组)51例和健康对照者30例.比较各组患者脑钠肽、和肤素水平与冠脉病变支数、冠脉Gensini评分相关性.结果.AMI组、UAP组脑钠肽、和肽素水平显著高于SAP组和对照组,且AMI组明显高于UAP组,差异均有统计学意义(P＜0.05).脑钠肽、和肽素水平与冠脉病变支数、左主干病变程度、冠脉Gensini评分呈正相关(P＜0.05).结论:脑钠肽、和肽素水平可反映冠心痛患者临床症状与冠脉病变的严重程度.     

急性冠状动脉综合症患者血浆溶血磷脂酸及基质金属蛋白酶-9 水平的检测及意义

目的:探讨急性冠状动脉综合症(acute coronary syndromes,ACS)患者血浆溶血磷脂酸(1ysophosphatidic acid,LPA)及基质金属蛋白酶-9(matrix metalloproteinase-9,MMP-9)水平的变化,探讨其在ACS中可能的临床意义。方法:入选100例ACS患者,根据病情将其分为急性心肌梗死(AMI)组50例,不稳定型心绞痛(UAP)组50例,并入选40例健康者作为对照组,分别用无机磷定量法和酶联免疫吸附测定法测定血浆LPA、MMP-9水平。结果:AMI组血浆LPA、MMP-9水平显著高于UAP组(LPA:3.29±0.42vs2.67±0.37;MMP-9:481.7±86.5vs237.85±65.34,P<0.01)并且两组均显著高于对照组(LPA:1.82±0.31;MMP-9:87.42±23.85P<0.01);LPA与MMP-9水平呈正相关(r=0.224,P<0.05)。结论:LPA与MMP-9可能是提示不稳定斑块的形成、破裂,进而导致急性冠状动脉综合征的危险信号。     

不稳定型心绞痛和急性心肌梗死患者血小板指标及超敏C 反应蛋白变化

目的:探讨不稳定型心绞痛(unstable angina pectoris,UAP)、急性心肌梗死(acute myocardial infarction,AMI)患者血小板指标、超敏C反应蛋白(C-reactive protein,hs-CRP)水平变化及意义。方法:选取于我院进行治疗的急性冠脉综合征患者300例中UAP患者121例(UAP组),AMI患者179例(AMI组)。另选100例健康体检者作为对照组。300例急性冠脉综合征患者均口服阿司匹林进行治疗。服用阿司匹林7 d后比较各组血小板指标血小板聚集率、血小板膜糖蛋白CD62p、尿11-脱氢-血栓素B2(11-dehydro-Thromboxane,11-DH-TXB2)及hs-CRP水平变化,同时记录随访1年急性冠脉综合征患者心血管事件发生情况。结果:治疗后UAP组、AMI组二磷酸腺苷(adenosine diphosphate,ADP)及花生四烯酸(arochidonic acid,AA)诱导的血小板聚集率、11-DH-TXB2与对照组差异显著(P0.05),AMI组CD62p、hs-CRP水平均显著高于对照组(P0.05);随访1年,11-DH-TXB2水平≥1500 ng/g者心血管事件发生率为51.02%,11-DH-TXB2水平1500 ng/g者心血管事件发生率为22.28%,二者发生率差异显著(P0.05)。结论:血小板指标、hs-CRP检测对UAP、AMI具有临床意义。     

川芎嗪对急性冠脉综合征患者血清C反应蛋白及血液流变学的影响

目的:观察川芎嗪注射液(LHI)对急性冠脉综合征(ACS)患者血清C-反应蛋白(CRP)及血液流变学的影响,探讨LHI在稳定冠脉粥样斑块中的作用.方法:本研究采用前瞻性开放性病例对照研究,以248例ACS患者(其中急性心肌梗死(AMI)115例,不稳定心绞痛(UA)133例)及100例健康志愿者为研究对象,与发生ACS48h空腹采静脉血,健康对照组空腹采静脉血,测定血清CRP及血液流变学指标.在248例ACS惠者中随机选取100例分为治疗组和对照组:对照组(50例)采用常规治疗;治疗组(50例)在常规治疗的基础上加LHI 80mg+5%葡萄糖250mi静脉滴注,一日一次.于治疗10d后检测患者CRP及血液流变学指标.结果:ACS患者血清CRP及血液流变学各指标均显著高于健康对照组(P＜0.01);AMI患者血清CRP及血液流变学各指标又显著高于UA组(P＜0.01).经治疗10d后,各组CRP及血液流变学各指标较治疗前均改善,差异有显著性(P＜0.01),治疗组血清CRP及血液流变学各指标显著低于对照组(P＜0.01).结论:ACS患者血清CRP增高,血液流变学指标异常.LHI可显著降低ACS患者血清CRP并改善血液流变学指标,提示其有抑制冠脉粥样斑块的炎性反应,稳定斑块,减少心血管不良事件的作用.     

冠脉PCI治疗后血浆脂蛋白相关磷脂酶A2与基质金属蛋白酶-9水平的变化及临床意义

目的:探讨冠脉经皮冠状动脉介入(PCI)治疗后血浆脂蛋白相关磷脂酶A2(LP-PLA2)与基质金属蛋白酶-9(MMP-9)水平的变化及其临床意义。方法:选择150例急性冠脉综合征(ACS)患者、128例稳定型心绞痛(SAP)患者及100例健康者分为作为ACS组、SAP组及对照组。比较三组入院时血浆MMP-9、LP-PLA2水平及ACS组经PCI治疗前后血浆MMP-9、LP-PLA2水平的变化。结果:与对照组比较,SAP组与ACS组的血浆MMP-9、LP-PLA2水平均明显增高(P0.05);与SAP组比较,ACS组明显增高(P0.05)。与术前比较,ACS组术后血浆MMP-9、LP-PLA2水平明显降低(P0.05)。MMP-9与LP-PLA2在ACS组血浆中呈显著正相关(r=0.617,P0.05),在SAP组与对照组中无相关性(P0.05)。结论:冠脉病变程度越严重,血浆MMP-9和LP-PLA2水平更高;PCI治疗后冠脉斑块趋于稳定,血浆MMP-9和LP-PLA2水平降低,且二者有相关性,提示MMP-9和LP-PLA2参与冠状动脉粥样硬化的发病与发展,且对预测ACS高危人群及评价疗效有一定的临床价值。     

冠心病患者血清IL-37水平及其与血清IL-6、IL-10、CRP水平的关系

目的:探讨不同类型冠心病患者血清白介素-37(IL-37)的水平及其与血清白介素-6(IL-6)、白介素-10(IL-10)、C反应蛋白(CRP)水平的关系。方法:选取急性心肌梗死患者20例(AMI组)、不稳定性心绞痛患者26例(UAP组)、稳定性心绞痛患者20例(SAP组)及冠脉造影正常者26例(CON组)为研究对象,采用酶联免疫吸附法(ELISA)测定其血清IL-37、IL-6、IL-10和CRP的水平并分析其相关性。结果:1UAP组、AMI组血清IL-37水平均较对照组(CON组)显著增高(p0.05),而SAP组与CON组比较无明显差异(P0.05)。2冠心病患者的血清IL-37水平与其血清CRP(r=0.3,P0.05)、IL-6(r=0.4,P0.05)水平均存在显著正相关性,与IL-10水平无明显相关(P=0.16)。当排除SAP组患者后,冠心病患者的血清IL-37水平与CRP(r=0.3,P0.05)、IL-6(r=0.5,P0.05)、IL-10(r=0.2,P0.05)水平均显著相关。结论:急性冠脉综合症(ACS)患者的血清IL-37水平显著升高,并与IL-6、IL-10、CRP水平相关,可能参与了ACS发病过程中的炎症反应。     

原发性高血压合并冠心病患者血清CRP的变化

目的:探讨原发性高血压合并不同类型冠心病患者血清C-反应蛋白(CRP)的变化及其意义。方法:用全自动生化分析仪检测71例原发性高血压患者和28例健康对照组人群的血清CRP水平,所有研究对象均行选择性冠状动脉造影检查。将原发性高血压组病人根据冠脉造影检查分为单纯原发性高血压组(EH),原发性高血压合并稳定性心绞痛组(EH+SAP)、原发性高血压合并不稳定心绞痛组(EH+UAP)、原发性高血压合并心肌梗死组(EH+MI),比较各组患者血清CRP水平的差异。结果:①与对照组比较,EH组及EH合并CAD各组患者血清CRP均显著升高(P0.05);②与EH组比较,EH+UAP和EH+MI组血清CRP水平均显著升高(P0.01);③与EH+SAP组比较,EH+AMI组和EH+UAP组血清CRP水平均显著升高(P0.01)。结论:冠心病合并原发性高血压患者血CRP水平与冠状动脉病变程度与斑块稳定程度存在正相关性。     

A polymorphism in the visfatin gene promoter is related to decreased plasma levels of inflammatory markers in patients with coronary artery disease

Visfatin, a newly identified proinflammatory adipokine, has been linked to coronary artery disease (CAD). The ?1535C>T polymorphism (rs61330082) located in the visfatin gene promoter is reportedly associated with proinflammatory status. However, it is unclear whether this polymorphism correlates with plasma levels of inflammatory markers including visfatin, hs-CRP, IL-6 and TNF-?? in CAD patients. The present study was to investigate the potential association of the ?1535C>T polymorphism with plasma levels of visfatin, IL-6, C reactive protein (hs-CRP) and TNF-?? in patients with CAD. We conducted a hospital based study with 171 CAD patients to examine the association between the ?1535C>T polymorphism and plasma levels of visfatin, hs-CRP, IL-6 and TNF-??. Plasma visfatin levels were markedly different between patients with stable angina pectoris (SAP, 11.91 ± 0.70 ng/l) and those with unstable angina pectoris (UAP, 17.49 ± 0.20 ng/l) or acute myocardial infarction (AMI, 16.63 ± 0.22 ng/l; SAP versus UAP or AMI, P < 0.05). Compared with the CC genotype, variant genotypes CT and TT correlated with significantly lower levels of visfatin, hs-CRP, IL-6 and TNF-?? in the SAP group (P < 0.05), with lower levels of hs-CRP and IL-6 in the UAP group (P < 0.05), and with lower levels of visfatin in the AMI group (P < 0.05) after adjustment for age, gender, smoking, hypertension, diabetes, dyslipidemia and medication. Our results suggest that the ?1535C>T polymorphism is associated with decreased plasma levels of inflammatory markers in CAD patients, reflecting that this polymorphism might provide a useful marker for predicting the development of CAD events.     

Interleukin-1B (-511) gene polymorphism is associated with acute coronary syndrome in the Turkish population

Objectives: acute coronary syndrome (ACS) is defined as an inflammatory disease associated with development of atherosclerosis and instability. IL-1 is a candidate inflammatory cytokine that is thought to trigger ACS. The purpose of this study was to determine the relationship between IL-1 gene family polymorphisms (IL-1RN, IL-1B in positions -511 and +3953) and ACS in the Turkish population. Methods: a total of 381 people participated in the study, with 117 control subjects and 264 ACS patients. Of the 264 ACS patients, 112 were diagnosed with stable angina pectoris (SAP) and 152 were diagnosed with unstable angina pectoris (USAP). The polymerase chain reaction (PCR) was used to determine the genotype of IL-1RN. The genotypes of IL-1B (-511 and +3953) were determined by PCR, followed by restriction enzyme digestion of the PCR products. Results: there were no significant differences in both IL-1RN, IL-1B (-511 and +3953) genotype distributions and IL-1RN allele frequencies between ACS patients and the control subjects. In addition, no association was observed in the allele frequency of IL-1B (-511 and +3953) between ACS patients and controls (p = 0.113 and p = 0.859, respectively), or between SAP patients and controls (p = 0.575 and p = 0.359, respectively). However, IL-1B allele 1 (C) (-511) polymorphism in USAP patients was found to be significantly different from that of control subjects (p = 0.041, OR: 2.01; 95% CI: 1.985-3.933). A significant difference was also observed between USAP and SAP patients for IL-1B (+3953) allele 1 (C) polymorphism; (p = 0.043, OR: 1.522; 95% CI: 1.012-2.88). Conclusion: these results show that IL-1RN gene polymorphism has no association with ACS. However, the allele 1 (C) of IL-1B (-511) may be a risk factor for susceptibility to USAP in the Turkish population.     

急性冠状动脉综合症患者血浆溶血磷脂酸及基质金属蛋白酶-9水平的检测及意义

目的：探讨急性冠状动脉综合症（acute coronary syndromes,ACS）患者血浆溶血磷脂酸（lysophatidic acid,LPA）及基质金属蛋白酶-9（matrix metalloproteinase-9,MMP．9）水平的变化,探讨其在ACS中可能的临床意义。方法：入选100例ACS患者,根据病情将其分为怠性心肌梗死（AMI）组50例,不稳定型心绞痛（UAP）组50例,并入选40例健康者作为对照组,分别用无机磷定量法和酶联免疫吸附测定法测定血浆LPA、MMP-9水平。结果：AMI组血浆LPA、MMP-9水平显著高于UAP组（LPA：3．29±0．42vs2．67±0．37;MMP-9：481．7±86．5VS237．85±65．34,P〈0．01）并且两组均显著高于对照组（LPA：1．82±0．31;MMP-9：87．42±23．85P〈0．01）;LPA与MMP-9水平呈正相关（r=O．224,P〈0．05）。结论：UIA与MMP-9可能是提示不稳定斑块的形成、破裂,进而导致急性冠状动脉综合征的危险信号。     

Pregnancy-associated plasma protein A and proform eosinophilic major basic protein in the detection of different types of coronary artery disease

Kryptor system was proven to be a rapid, standard method for pregnancy-associated plasma protein A and proform eosinophilic major basic protein (PAPP-A/proMBP) complex detection in coronary artery disease (CAD). No age and/or gender differences in 51 controls and 110 stable coronary artery disease (SCAD) patients were found. SCAD patients did not differ from controls and no difference in PAPP-A/proMBP levels with regards to the number of affected vessels was found. In 21 unstable angina pectoris (UAP), in 35 without and 66 with ST elevation acute myocardial infarctions (NSTEMI, STEMI respectively) patients PAPP-A/proMBP levels were increased (P=0.004 and P<0.0005, respectively). PAPP-A/proMBP levels did not correlate with cardiac troponin I (cTnI) in STEMI and NSTEMI patients. PAPP-A/ proMBP increase was more frequent than cTnI (P=0.036) within the early phase of STEMI. In NSTEMI patients PAPP-A/proMBP positivity was present in 50 % of cTnI negative cases. Receiver operating characteristic (ROC) analysis revealed the highest diagnostic accuracy of PAPP-A/proMBP (0.919) in STEMI cTnI positive cases. The highest specificity/sensitivity PAPP-A/proMBP levels for particular acute coronary syndrome (ACS) types were 10.65-14.75 mIU/l. Combination of PAPP-A/proMBP with cTnI increases their diagnostic efficacy within the early phase of ACS. Our results suggest that PAPP-A/proMBP complex is involved in processes preceding vulnerable plaque development in ACS.     

老年急性冠脉综合征患者血尿酸与血脂、HCY及hs-CRP水平的关系研究

目的:研究老年急性冠脉综合征(ACS)患者血尿酸(UA)与血脂、同型半胱氨酸(HCY)及超敏C反应蛋白(hs-CRP)水平的关系。方法:选择从2014年2月到2017年9月在重庆三峡中心医院接受治疗的老年ACS患者59例作为研究对象。急性心肌梗死(AMI)患者32例,即为AMI组;不稳定型心绞痛(UAP)患者27例,即为UAP组。另选同期30例在我院接受体检的健康志愿者作为对照组,对比各组UA、HCY、hs-CRP水平以及血脂水平,并分析患者UA与血脂、HCY及hs-CRP水平的相关性。结果:AMI组和UAP组的UA、HCY及hs-CRP水平均高于对照组,且AMI组又高于UAP组(P0.05)。AMI组和UAP组的总胆固醇(TC)、甘油三酯(TG)、低密度脂蛋白(LDL)水平均高于对照组,AMI组又高于UAP组(P0.05);AMI组和UAP组的高密度脂蛋白(HDL)水平低于对照组,且AMI组又低于UAP组(P0.05)。根据Spearman法分析相关性可知,患者UA与HCY、hs-CRP、TC、TG及LDL均呈正相关,与HDL呈负相关(P0.05)。结论:老年ACS患者UA、HCY、hs-CRP、TC、TG及LDL水平均较高,HDL水平较低,且UA与血脂、HCY以及hs-CRP均具有较好的相关性,临床可尝试将其作为重点监测靶点,从而应用于ACS患者的诊治。     

冠心病患者体内瘦素与高敏C反应蛋白检测的临床意义

目的:研究血清瘦素(LP)与高敏C反应蛋白(hsCRP)水平在冠心病患者体内的变化,及临床检测意义。方法:入选住院确诊的冠心病住院患者168例,对照组62例。外周血白细胞(WBC)水平应用全自动血细胞计数仪检测,血清高敏C反应蛋白水平应用比浊法检测,并血清瘦素水平用酶联免疫吸附法检测,分析瘦素与高敏C反应蛋白和外周血白细胞的关系。结果:(1)冠心病稳定型心绞痛组、不稳定型心绞痛组及急性心肌梗死组血清瘦素、高敏C反应蛋白和外周血白细胞水平显著高于对照组(均P0.05)。(2)血浆瘦素、高敏C反应蛋白及外周血白细胞水平在稳定型心绞痛组、不稳定型心绞痛组及急性心肌梗死组三组间依次增高,差异有统计学意义(均P0.05)。(3)简单相关分析显示瘦素与高敏C反应蛋白和外周血白细胞相关,r值分别为5.241和4.025,均P0.05。结论:瘦素与高敏C反应蛋白和外周血白细胞关系密切,可能通过炎症反应参与冠心病的发生。     

Elevated Levels of Oxidized Low-Density Lipoprotein Correlate Positively with C-Reactive Protein in Patients with Acute Coronary Syndrome

The relationship between oxidized low-density lipoprotein (Ox-LDL) and C-reactive protein (CRP) in patients with acute coronary syndrome (ACS) is unknown. We, therefore, measured serum levels of Ox-LDL and high-sensitivity (hs)-CRP in 90 ACS patients, 45 stable angina pectoris (SAP) patients, and 66 healthy controls using sandwich ELISA. ACS patients were subdivided into: (1) acute myocardial infarction (AMI; n = 45); (2) unstable angina pectoris (UAP; n = 45) groups. In AMI patients, Ox-LDL (177.5 mmol/l) and hs-CRP (25.40 mg/l) levels were significantly higher (P < 0.01) than in UAP (Ox-LDL:107.5 mmol/l, hs-CRP:10.7 mg/l) and SAP (Ox-LDL:82.3 mmol/l, hs-CRP:2.10 mg/l) patients as well as controls (Ox-LDL:41.4 mmol/l, hs-CRP:1.76 mg/l). Ox-LDL/hs-CRP levels in UAP patients were significantly higher (P < 0.01) than in SAP patients and controls. Importantly, a positive correlation was found between Ox-LDL and CRP (r = 0.622; P < 0.01) levels. Serum levels of total, HDL, and LDL cholesterol did not differ among these patient groups. In conclusion, our data show that Ox-LDL and hs-CRP levels correlate positively in ACS patients, supporting the hypothesis that Ox-LDL and CRP may play a direct role in promoting the inflammatory component of atherosclerosis in these individuals. We suggest that Ox-LDL/CRP elevated levels may serve as markers of the severity of the disease in evaluation and management of ACS patients.     

中国汉族人群DNA酶I基因多态性与不稳定性心绞痛的关系

目的：探讨脱氧核糖核酸酶（IDNA酶I）基因多态性与汉族人群不稳定性心绞痛（unstable angina pectoris,UAP）易感性的关系。方法：以196例UAP患者为病例组,排除冠心病的297例体检者为对照组,应用PCR及PCR-限制性片段长度多态（PCR-RFLP）分析DNA酶I基因8外显子单核苷酸多态位点A2317G及4内含子56bp可变串联重复序列（HumDN1）多态性;协方差分析A2317G、HumDN1各基因型与UAP患者血脂的关系,将年龄、性别、高血压、糖尿病及吸烟作为协变量;x2检验分析UAP患者冠脉血管病变支数与DNA酶I基因型的关系。结果：UAP组与对照组A2317G、HumDN1各基因型及等位基因分布无明显统计学差异（P〉0.05）,两组DNA酶I单体型分布亦无差异。UAP患者DNA酶I各基因型血脂水平、冠脉血管病变支数的差异无明显统计学意义,所有P值均〉0.05。结论：DNA酶I基因多态性与中国汉族人群不稳定心绞痛及其血脂水平无明显相关性。     

Immunoglobulin E and matrix metalloproteinase-9 in patients with different stages of coronary artery diseases

The aims of this study were to identify levels of total immunoglobulin E (IgE) and matrix metalloproteinase (MMP)-9 in patients with different stages of coronary artery diseases. IgE, MMP-9, creatine phosphokinase (CPK), lactate dehydrogenase (LDH), total cholesterol, low density lipoprotein cholesterol (LDL), high density lipoprotein cholesterol (HDL) and triglyceride (TG) were measured by fluorescence enzyme immunoassay, gelatin zymography, and autoanalyzer in normal subjects (n = 40), patients with stable angina pectoris (SAP, n = 40), patients with unstable angina pectoris (UAP, n = 40), patients with acute myocardial infarction (AMI, n = 40), or post-CABG-surgery of those acute myocardial infarction (P-CABG, n = 40). Compared with normal subjects, increased IgE but unchanged MMP-9, CPK, LDH were found in SAP group and UAP group, whereas IgE, MMP-9, CPK and LDH levels were all significantly increased in AMI group. IgE, MMP-9, CPK and LDH levels in P-CABG group were significantly reduced, compared with AMI group, and were similar to those in normal subjects. Cholesterol, LDL, HDL and TG were not significantly changed in all groups. We suggest that serum total IgE can be an early marker of coronary artery disease and MMP-9 is a marker of acute myocardial infarction.