Screening for mutations in human cardiomyopathy- is RBM24 a new but rare disease gene?

With great interest we have read the study of Liu et al.(2018) revealing the role of RNA binding protein 24 (RBM24) on global alternative splicing and dilated cardiomyopathy (DCM) in mice. As suggested previously, deficiency of Rbm24 causes embryonic lethality limiting the functional analyses (Yang et al., 2014). To circumvent this limitation the authors generated cardiac specific Rbm24 deficient mice and showed that homozygous deletion of Rbm24 at postnatal stage leads to rapidly progressive DCM and heart failure (Liu et al., 2018).     

1993年度微生物学及其相关学科国际学术活动日程表

124徽生物学通报1993年20(2)1993年度徽生物学及其相关学科国际学术活动日程表1月4一8日l组DNA技术方法学会议(美国,华盛倾)9一11日PCR技术会议(美国,华盛倾)9一15日植物细胞外墓质:分子、细胞和发育生物学会 议(美国,新且西哥州)11一16日蛋白质运移、翰出和加工(奥地利,安纳贝尔 /萨茨份)14一15日遗传报伤和不受多育控制(瑞士,洛桑)18一21日蛋白质提纯和生化工程会议(美国,圣菲)17一22日墓因技术进展:蛋白质工程及其他(美国,迈 阿密)17一23日迈阿密生物工程学冬季讨论会:大分子设计和 功能(美国,迈阿密)17一24日转录因子、调节和分化会…     

Interleukin-25 in sputum as a marker of airway inflammation in children asthma

<正>Dear Editor,Interleukin-25 (IL-25, also called IL-17E) is an important IL-17 family member that helps initialize and regulate type 2immunity, exerting an important influence on the pathogenesis of asthma (Corrigan et al., 2011; Barlow et al., 2012).Furthermore, it plays a vital role in initiating type 2 innate lymphoid cell activation and maintenance (Tong and Li,2018) and inducing Th2-type cytokine-mediated allergic airway eosinophilia (Morita et al., 2015). IL-25 expression     

Interspecies embryo transfer between rhesus and cynomolgus monkeys

正Nonhuman primates (NHPs) present significant advantages over rodents for physiological and pharmacological investigations because of their higher genetic and physiological similarity to humans. Both rhesus (Macaca mulatta) and cynomolgus (Macaca fascicularis) monkeys are considered ideal animal models in biomedical research (Phillips et al., 2014), especially in modeling normal development and diseases in the nervous system (Izpisua Belmonte et al., 2015; Ng et al., 2015; Niu et al., 2015; Poo et al.,     

Asthma-associated polymorphisms in 17q12-21 locus modulate methylation and gene expression of GSDMA in naive CD4~+ T cells

正The 17q12-21 locus harboring ORMDL sphingolipid biosynthesis regulator 3 (ORMDL3) and Gasdermin B (GSDMB) genes exhibits one of the most significant and replicated associations with asthma[MIM:600807](Stein et al.,2018).Several studies explored the complex interactions between SNPs,gene expression and epigenetic modifications at this locus,primarily focusing on the levels of expressed ORMDL3 and GSDMB (Stein et al.,2018).Given that DNA methylation and gene expression signals are cell-type specific(Reinius et al.,2012),isolated materials such as lymphocytes from blood and,to a lesser extent,bronchial epithelial cells from biopsies of bronchi or bronchoalveolar lavages have been used in a number of studies to investigate such interactions (Stein et al.,2018).None     

A modified high-efficiency thermal asymmetric interlaced PCR method for amplifying long unknown flanking sequences

<正>Rapid and efficient isolation of unknown flanking DNA sequences adjacent to known regions is important for molecular biology research.For this purpose,several PCR-based methods have been reported,including inverse PCR(Uchiyama and Watanabe,2006),ligation-mediated PCR(Yan et al.,2003;Ballester et al.,2005;Wang et al.,2007;Trinh et al.,2012)and randomly primed PCR(Liu and Whittier,1995;Liu et al.,1995;Antal et al."2004;Liu and Chen,2007;Reddy et al.,2008;Wang     

Epigenetic Variations in Plant Hybrids and Their Potential Roles in Heterosis

Heterosis,one of the most important biological phenomena,refers to the phenotypic superiority of a hybrid over its genetically diverse parents with respect to many traits such as biomass,growth rate and yield.Despite its successful application in breeding and agronomic production of many crop and animal varieties,the molecular basis of heterosis remains elusive.The classic genetic explanations for heterosis centered on three hypotheses:dominance (Davenport,1908;Bruce,1910;Keeble and Pellew,1910;Jones,1917),overdominance (East,1908;Shull,1908) and epistasis (Powers,1944;Yu et al.,1997).However,these hypotheses are largely conceptual and not connected to molecular principles,and are therefore insufficient to explain the molecular basis of heterosis (Birchler et al.,2003).Recently,many studies have explored the molecular mechanism of heterosis in plants at a genome-wide level.These studies suggest that global differential gene expression between hybrids and parental lines potentially contributes to heterosis in plants (e.g.,Swanson-Wagner et al.,2006;Zhang et al.,2008;Wei et al.,2009;Song et al.,2010).Research suggests that genetic components,including cis-acting elements and trans-acting factors,are critical regulators of differential gene expression in hybrids (Hochholdinger and Hoecker,2007;Springer and Stupar,2007;Zhang et al.,2008).However,other research indicates that epigenetic components,the regulators of chromatin states and genome activity,also have the potential to impact heterosis (e.g.,Ha et al.,2009;He et al.,2010;Groszmann et al.,2011;Barber et al.,2012;Chodavarapu et al.,2012;Greaves et al.,2012a;Shen et al.,2012).     

论寒武系馒头组三叶虫群星虫属(Asteromajia)北大核心CSCD

根据山东长清县张夏镇馒头山馒头组采集到的三叶虫新材料,重新厘定和恢复群星虫(Asteromajia Nan and Chang,1982)作为裂头虫科内有效属名的地位,将徐庄虫(Hsuchuangia Lu and Zhu in Qiu et al.,1983),八公山虫(Bagongshania Lin in Qiu et al.,1983)作为群星虫的晚出异名。修订我国以往所描述的毛庄期和徐庄期4个属(Kochaspis Resser,1935,Asteromajia Nan and Chang,1982,Hsuchuangia Lu and Zhu in Qiu et al.,1983,Bagongshania Lin in Qiu et al.,1983)内的15个种的特征和头尾搭配,归并为4属,10种:Temnoura huoshanensis(Zhang and Wang,1985),Zhongtiaoshanaspis angustilimbata(Meng in Zhou et al.,1977),Solenoparia funingensis(An in Duan et al.,2005),Asteromajia hsuchuangensis(Lu in Lu and Dong,1952),A.quadrata(Resser and Endo,1937),A.liuheensis(An,1966),A.huainanensis(Lin in Qiu et al.,1983),A.lüliangshanensis(Zhang and Wang,1985),A.?yangchengensis(Zhang and Wang,1985),A.?longiceps(Lu and Zhu,2001),其中后2个种的归属尚有疑问。     

论寒武系馒头组三叶虫群星虫属(Asteromajia)

根据山东长清县张夏镇馒头山馒头组采集到的三叶虫新材料,重新厘定和恢复群星虫(Asteromajia Nan and Chang,1982)作为裂头虫科内有效属名的地位,将徐庄虫(Hsuchuangia Lu and Zhu in Qiu et al.,1983),八公山虫(Bagongshania Lin in Qiu et al.,1983)作为群星虫的晚出异名。修订我国以往所描述的毛庄期和徐庄期4个属(Kochaspis Resser,1935,Asteromajia Nan and Chang,1982,Hsuchuangia Lu and Zhu in Qiu et al.,1983,Bagongshania Lin in Qiu et al.,1983)内的15个种的特征和头尾搭配,归并为4属,10种:Temnoura huoshanensis(Zhang and Wang,1985),Zhongtiaoshanaspis angustilimbata(Meng in Zhou et al.,1977),Solenoparia funingensis(An in Duan et al.,2005),Asteromajia hsuchuangensis(Lu in Lu and Dong,1952),A.quadrata(Resser and Endo,1937),A.liuheensis(An,1966),A.huainanensis(Lin in Qiu et al.,1983),A.lüliangshanensis(Zhang and Wang,1985),A.?yangchengensis(Zhang and Wang,1985),A.?longiceps(Lu and Zhu,2001),其中后2个种的归属尚有疑问。     

Breeding cotton with superior fiber quality: identification and utilization of multiple elite loci and exotic genetic resources

正In the last decade, cotton genomics had great progress in genome assembling(Li et al., 2015; Huang et al., 2020) and agriculturally important locus identification(Du et al., 2018;Ma et al., 2018; Li et al., 2021).Upland cotton(Gossypium hirsutum) is one of the most important cash crops cultivated worldwide. The fiber quality of cotton directly determines its economic value, and therefore enhancing fiber quality is always the primary task in cotton breeding practices(Xia et al., 2019; Huang et al.,2021).     

The post-genomics era of cotton

正As one of the important industrial crops and also an excellent model organism for studying cell elongation,cell wall biosynthesis as well as polyploidy evolution,cotton has always been in the limelight of the world(Shi et al.,2006;Qin and Zhu,2011).With the publication of the allotetraploid Gossypium hirsutum and Gossypium barbadense genomes this year(Li et al.,2015;Zhang et al.,2015;Liu et al.,2015),all three important cotton genomes,including the two ancestor diploid Gossypium raimondii(DD)(Wang et al.,2012;Paterson et al.,2012)and Gossypium arboreum(AA)(Li et al.,2014)have been successfully sequenced by scientists from China and from the United States of America.Since functional studies of Arabidopsis thaliana and     

Structural basis of AimP signaling molecule recognition by AimR in Spbeta group of bacteriophages

Dear Editor,Quorum sensing(QS)is a widespread phenomenon in bacteria which enables them to participate in cell-to-cell communication by producing and responding to small signal molecules,thus synchronously altering their behavior depending on population density(Singh et al.,2000;Miller and Bassler,2001).Through QS,bacteria coordinate processes such as expression of virulenee factors(Slamti and Lereclus,2002),biofilm formation(Parashar et al.,2011),sporulation(Perego et al.,1996),conjugation(Kozlowicz et al.,2006),antibiotic synthesis(Miller and Bassler,2001;Whiteley et al.,2017)etc.     

土耳其斯坦东毕吸虫的扫描电镜观察

血吸虫类中如血居科(sanguinicolidae)的Aporocotyle simplex Odhner,1900、裂体科(Schistosomatidae)的日本血吸虫(Schistosoma japonicum)曼氏血吸虫(S.mansoni)及埃及血吸虫(S.haematobium)等多种血吸虫均经扫描电镜观察(Johnson and Moriearts,1969;Silk et al., 1969;Robson and Erasmus, 1970; Miller et al., 1972; Kuntz et al.,1976、1977;Voge et al., 1978; Thulin, 1980;及何毅勋和马金鑫,1980等)。关于土耳其斯坦东毕吸虫[Orientobitharzia lurkestanica(skrjabin,1913)Dut et Srivastava,1955]的体表扫描电镜尚无报告,而只见有此虫种体壁及肠管的透射电镜观察的资料(Lavrov and Fedoseenke,1978)。本文部分作者最近在内蒙东部兴安岭以南部分地区进行牛羊土     

Plant genome editing using xCas9 with expanded PAM compatibility

CRISPR/Cas enables robust genome editing and has revolution-ized both functional genomics and crop breeding.The specificity of Cas-directed DNA cleavage is strictly determined by a chimeric single guide RNA(SgRNA)and a short protospacer adjacent motif(PAM)in the genome(Cong et al,2013;Zetsche et al,2015).The widely used Cas9 from Streptococcus pyogenes(SpCas9)generally recognizes the canonical NGG PAM(where N indicates any nucleicacid base)(Miao et al.,2013;Ma et al,2015),making many regionsuntargetable by Cas9.SpCas9 VQR and VRER variants,which recog-nize the non-canonical PAM sequences of NGA and NGCG,respectively,have been used to expand targetable sequences in plants(Hu et al.,2016).In addition,the applications of other Cas endonucleases such as SaCas9(Staphylococcus aureus Cas9)(Ran et al..2015).     

SOCS-3 contributes to bisphenol a exposure-induced insulin resistance in hepatocytes

<正>Dear Editor,Bisphenol A (BPA), a common and widely used monomer in the production of polycarbonate plastics and epoxy resin,(Healy et al., 2015; De Filippis et al., 2018) has been defined as an endocrine disrupting chemical (EDC) associated with the etiology of insulin resistance (IR)(Gioiosa et al., 2015;Wei et al., 2017) and metabolic disorders (Padmanabhan et al., 2010; Fang et al., 2015; Balistrieri et al., 2018). However,     

Beneficial effects exerted by hydroxychloroquine in treating COVID-19 patients via protecting multiple organs

正Dear Editor.The coronavirus disease 2019 (COVID-19) pandemic caused by widespread infection with the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)(Chen et al.,2020;Guan et al.,2020) has led to a global health crisis.More than 3.5 million infections and 246,838 deaths have occurred as of May 4,2020 (Saqrane and El Mhammedi,2020),with a rapid upward trend.Many CO VID-19 patients suffer from complicated systemic injuries such as cardiac(Chen et al.,2020) and hepatic (Huang et al.,2020) damages.There are currently no specific drugs on the market to treat COVID-19-induced systemic injuries (Ni et al.,     

Multilayer network analysis: new opportunities and challenges for studying animal social systems

Introduction Many behavioral,ecological,and evolutionary processes are closely intertwined with patterns of social interactions,such as the evolution of cooperation(Croft et al.2006),information and disease transmission(VanderWaal et al.2014;Aplin et al.2015),predator-prey dynamics(Ioannou et al.2012),and dispersal decisions(Blumstein et al.2009).Even in species where individuals are traditionally viewed as leading a relatively solitary existence,interac-tions occur across diverse contexts,including territorial defense,resource competition,and courtship.     

Structure-activity relationship optimization for lassa virus fusion inhibitors targeting the transmembrane domain of GP2

Dear Editor,Lassa virus(LASV)belongs to the Mammarenavirus genus,Arenaviridae family.Arenaviruses are classified into two main groups-Old World(OW)and New World(NW)-based on virus genetics,serology,antigenic properties and geographical relationships.The OW LASV and Lujo virus(LUJV),as well as NW Jurn'n virus(JUNV),Machupo virus(MACV),Guanarito virus(GTOV),Sabia virus(SABV)and Chapare virus(CHAPV),are known to cause severe hemorrhagic fever and are listed as biosafety level 4(BSL-4)agents.The arenavirus glycoprotein complex(GPC)contains three subunits-the retained stable-signal peptide(SSP),the receptor-binding subunit GP1,and the membrane fusion subunit GP2(Lenz et al.,2001).Notably,the proximate external membrane region and TM of GP2,together with the ectodomain loop and TMs of SSP,form an SSP-GP2 interface,playing essential roles in regulating membrane fusion,and providing targets for distinct fusion inhibitors(Larson et al.,2008;Lee et al.,2008;York et al.,2008;York and Nunberg,2009;Thomas et al.,2011;Burgeson et al.,2013a;Shankar et al.,2016;Wang et al.,2016;Wang et al.,2018).Among these inhibitors,ST-161 is LASV specific(Burgeson et al.,2013a).In this study,we conducted structure-activity relationship(SAR)optimization of ST-161.As a result,21 derivatives with IC50 values<1 pmol/L are presented in Table S1.Hit compounds 21,29 and 57 exhibiting robust inhibition of the LASV pseudotype virus(LASVpv,VSV backbone enveloped by LASV GPC with single cycle infection)entry with IC50 values lower than 0.2 nmol/L(Figs.1A and S1),as well as hit compound 72 with an ester bond instead of acylhydrazone,were further investigated.To test whether the four hit compounds inhibit LASV entry by blocking the GPC-mediated membrane fusion,the inhibition effects of these compounds against LASV GPC mediated fusion were quantitatively determined by dual-luciferase assay(Thomas et al.,2011;Wang et al.,2018).