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1.
A bacterial strain, designated KMM 6244T, was isolated from the sea urchin Strongylocentrotus intermedius and subjected to a polyphasic taxonomic investigation. The bacterium was found to be heterotrophic, aerobic, non-motile and
spore-forming. Comparative phylogenetic analysis based on 16S rRNA gene sequencing placed the marine isolate in the genus
Bacillus. The nearest neighbor of strain KMM 6244T was Bacillus decolorationis LMG 19507T with a 16S rRNA gene sequence similarity of 98.0%. Sequence similarities with the other recognized Bacillus species were less than 96.0%. The results of the DNA–DNA hybridization experiments revealed a low relatedness (37%) of the
novel isolate with the type strain of B. decolorationis LMG 19507T. Strain KMM 6244T grew at 4–45°C and with 0–12% NaCl. It produced catalase and oxidase and hydrolyzed aesculin, casein, gelatin and DNA. The
predominant fatty acids were anteiso-C15:0, iso-C15:0, anteiso-C17:0, C15:0, iso-C16:0 and iso-C14:0. The DNA G + C content was 39.4 mol%. A combination of phylogenetic, genotypic and phenotypic data clearly indicated that
strain KMM 6244T represents a novel species in the genus Bacillus, for which the name Bacillus berkeleyi sp. nov. is proposed. The type strain is KMM 6244T (KCTC 12718T = LMG 26357T). 相似文献
2.
Byung-Chun Kim Kang Hyun Lee Mi Na Kim Min Young Jung Young-Hyo Chang Jongtae Lee Kee-Sun Shin 《Current microbiology》2011,62(2):606-611
Strain M1-2T was isolated from the black sand from the seashore of Jeju Island, Republic of Korea and was classified using a polyphasic
taxonomic approach. Strain M1-2T appeared as Gram-negative, motile rods that could grow in the presence of 1–10% (w/v) NaCl and at temperatures ranging from
4 to 37°C. This isolate has catalase and oxidase activity and hydrolyses aesculin, DNA and l-tyrosine. Based on phylogenetic analysis using 16S rRNA gene sequences, strain M1-2T belongs to the genus Joostella and is clearly distinct from the other described species of this genus, Joostella marina (type strain En5T). The 16S rRNA gene sequence similarity level between M1-2T and J. marina En5T is 97.2%, and the DNA–DNA relatedness value between the two strains is 23.9%. Strain M1-2T contains MK-6 as the major menaquinone and iso-C15:0, summed feature 3 (C16:1 ω7c and/or iso-C15:0 2OH) and iso-C17:0 3OH as major cellular fatty acids. The DNA G + C content is 32.3 mol%. These data suggest that strain M1-2T should be classified as a novel species, for which the name Joostella atrarenae sp. nov. is proposed. The type strain for the novel species is M1-2T (= KCTC 23194T = NCAIM B.002413T). 相似文献
3.
You-Sung Oh Sang-Bin Lim Hyung-Yeel Kahng Byoung-Jun Yoon Duck-Chul Oh Jae-Ho Joa Dong-Heon Lee 《Journal of microbiology (Seoul, Korea)》2010,48(3):307-311
A novel marine bacterium, designated strain CNURIC014T was isolated from coastal seawater of Jeju Island in Korea. Strain CNURIC014T formed yellow colonies on marine agar 2216 and the cells were Gram-negative, non-motile, strictly aerobic, rod-shaped. The
temperature, pH and NaCl ranges for growth were 15–37°C, pH 6.0–9.0 and 1.0–7.0% NaCl. Phylogenetic analysis based on 16S
rRNA gene sequences indicated that strain CNURIC014T was most closely related to Gaetbulibacter marinus and Gaetbulibacter saemankumensis, with a sequence similarity of 95.1% and 94.6%, respectively. The DNA G+C content of the strain was 33.1 mol% and the major
respiratory quinone was menaquinone-6. The major cellular fatty acids were iso-C15:1 (22.8%), iso-C15:0 (18.8%), summed feature 3 (iso-C15:0 2-OH/C16:1 ω7c, 12.9%) and iso-C17:0 3-OH (11.5%). On the basis of phenotypic, phylogenetic, and genotypic data, strain CNURIC014T represents a novel species within the genus Geatbulibacter, for which the name Gaetbulibacter jejuensis sp. nov. is proposed. The type strain is CNURIC014T(=KCTC 22615T =JCM 15976T). 相似文献
4.
Shengwen Duan Zhengchu Liu Xiangyuan Feng Ke Zheng Lifeng Cheng 《Journal of microbiology (Seoul, Korea)》2009,47(6):693-698
A Gram-negative, non-motile, non-spore-forming bacterial strain designated IBFC2009T was isolated from soil of a bamboo plantation. The strain could grow at 11°C∼39°C, pH 6.0–9.0, and in the presence of 0∼5%
NaCl. Based on 16S rRNA gene sequence analysis, Strain IBFC2009T belonged to the genus Sphingobacterium and showed the highest sequence similarity of 94.6% (S. composti T5-12T) with the type strains within the genus. The major fatty acids were summed feature 3 (iso-C15:0 2-OH and/or C16:1
ω7c, 34.4%), iso-C15:0 (22.4%), C16:0 3-OH (15.2%), and iso-C17:0 3-OH (12.8%). The G+C content of the genomic DNA was 41.0 mol%. According to the phenotypic and genotypic characteristics,
Strain IBFC2009T should represent a novel species of the genus Sphingobacterium, for which the name Sphingobacterium bambusae sp. nov. is proposed. The type strain is IBFC2009T (=CCTCC AB 209162T =KCTC 22814T). 相似文献
5.
Taxonomic studies were performed on three strains isolated from Cheonho reservoir in Cheonan, Korea. The isolates were Gram-negative,
aerobic, rod-shaped, non-motile, catalase-positive, and oxidase-positive. Colonies on solid media were cream-yellow, smooth,
shiny, and circular. Phylogenetic analysis of the 16S rRNA gene sequences revealed that these strains belong to the genus
Flavobacterium. The strains shared 98.6–99.4% sequence similarity with each other and showed less than 97% similarity with members of the
genus Flavobacterium with validly published names. The DNA-DNA hybridization results confirmed the separate genomic status of strains ARSA-42T, ARSA-103T, and ARSA-108T. The isolates contained menaqui-none-6 as the predominant menaquinone and iso-C15:0, iso-C15:0 3-OH, iso-Ci15:1 G, and iso-C16:0 3-OH as the major fatty acids. The genomic DNA G+C content of the isolates were 31.4–33.2 mol%. According to the phenotypic
and genotypic data, these organisms are classified as representative of three novel species in the genus Flavobacterium, and the name Flavobacterium koreense sp. nov. (strain ARSA-42T =KCTC 23182T =JCM 17066T =KACC 14969T), Flavobacterium chungnamense sp. nov. (strain ARSA-103T =KCTC 23183T =JCM 17068T =KACC 14971T), and Flavobacterium cheonanense sp. nov. (strain ARSA-108T =KCTC 23184T =JCM 17069T =KACC 14972) are proposed. 相似文献
6.
A Gram-negative, non-motile, rod shaped, and orange-pigmented chemoheterotrophic bacterium, strain MS-31T was isolated from the marine sponge Hymeniacidon flavia, collected from near Jeju Island, Korea. The Strain MS-31T was subjected to a polyphasic taxonomic study. The phylogenetic analysis based on the 16S rRNA gene sequences revealed that
the novel isolate could be affiliated within the genus Sphingomonas. The strain MS-31T showed 95.6% of 16S rRNA gene sequence similarity with the most closely related species Sphingomonas koreensis JSS26T. The DNA G+C content of the strain MS-31T was 69.4 mol%. The major isoprenoid quinone was ubiqunone 10 and predominant cellular fatty acids were summed feature 7 (comprising
C18:1 ω7c, C18:1 Ω9t and/or C18:1 ωl2t, 39.7%), C16:0 (16.3%), C14:0 2OH (15.9%) and summed feature 3 (comprising C16:1 ω7c and/or C15:0 iso 2OH, 11.7%). The polar lipids were sphingoglycolipid, phosphatidyletha-nolamine, phosphatidylglycerol, diphosphatidylglycerol
and unidentified glycolipid. Based on the evidence from the polyphasic taxonomic study, the strain should be classified as
a new species of the genus Sphingomonas. As a result, the name Sphingomonas jejuensis sp. nov. (type strain MS-31T =KCTC 23321T =NBRC 107775T) is proposed. 相似文献
7.
An aerobic, Gram-negative bacterial strain, designated KU27E1T, which degrades phthalate and dimethylphthalate, was isolated from seawater obtained from the coastal region of Ishigaki
Island, Japan. Cells are motile rods with polar flagella. Strain KU27E1T grew at 15–30°C, pH 6.0–8.0, in the presence of 1.0–2.0% (w/v) NaCl. The 16S rRNA gene sequence analysis revealed that this
strain was affiliated with the family Rhodobacteraceae in the class Alphaproteobacteria, and was most closely related to Tropicibacter naphthalenivorans (96.8%). The predominant respiratory lipoquinone was ubiquinone-10, and the major cellular fatty acid was C18:1ω7c (88.5%). The G+C content of genomic DNA was 58.7 mol%. Based on the physiological, chemotaxonomic, and phylogenetic data,
strain KU27E1T is suggested to represent a novel species of the genus Tropicibacter, for which the name Tropicibacter phthalicus sp. nov. is proposed. The type strain of Tropicibacter phthalicus is designated as KU27E1T (=JCM 17793T = KCTC 23703T). 相似文献
8.
9.
Zhe-Xue?Quail Dan-Ning?Zeng Yi-Ping?Xiao Seong?Woon?Roh Young-Do?Nam Ho-Won?Chang Jung-Hoon?Yoon Hee-Mock?Oh Jin-Woo?Bae
A bacterial strain, designated Iso4T, was isolated from the East Sea of Korea and was subjected to a poly-phasic taxonomy study including phenotypic and chemotaxonomic
characteristics as well as 16S rRNA gene sequence analysis. Cells of the strain were Gram-negative, motile, non-budding, non-stalked,
and strictly aerobic. Strain Iso4T grew optimally at 20°C in the presence of 1∼2% (w/v) NaCl and at pH 6.9∼7.6. The major respiratory quinone was Q-10 and the
major cellular fatty acids were C18:1 ω7c (53.5%), C17:1 ω5c (11.7%), C17:1 ω6c (8.1%), C16:0 (7.8%), C17:0 (4.8%), C15:0 (2.9%), and C16:1 ω5c (2.2%). The DNA G+C content of strain Iso4T was 56.2 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain Iso4T formed a monophyletic clade in the family Hyphomonadaceae, supported by high bootstrap value and was most closely related to the genus Hyphomonas (92∼94%), a member of marine bacteria in the family. The phenotypic, genotypic, and chemotaxonomic evidences also suggest
strain Iso4T represents a novel genus and species in the family Hyphomonadaceae, for which the name Henriciella gen. nov., sp. nov. is proposed. The type strain is Iso4T (=KCTC 12513T =DSM 19595T =JCM 15116T). 相似文献
10.
Yi-Guang Chen Shu-Kun Tang Yu-Qin Zhang Zhao-Yang Li Lang-Bo Yi Yong-Xia Wang Wen-Jun Li Xiao-Long Cui 《Antonie van Leeuwenhoek》2009,96(1):63-70
A novel Gram-positive, halotolerant, non-sporulating, non-motile, catalase-positive, oxidase-negative and aerobic bacterium,
designated strain JSM 078085T, was isolated from sea water collected from the South China Sea. Strain JSM 078085T exhibited a rod-coccus growth cycle and produced a yellow pigment. The strain was able to grow in the presence of 0–12% (w/v)
NaCl and at pH 6.0–9.5 and 4–35°C; optimum growth was observed at pH 7.0 and 25–30°C in the absence of NaCl. The peptidoglycan
type was A4α (l-Lys–l-Ala–l-Glu). Cell-wall sugars contained galactose and glucose. Strain JSM 078085T contained menaquinone MK-9(H2) as the major respiratory quinone and diphosphatidylglycerol, phosphatidylglycerol and phosphatidylinositol as the major
polar lipids. The major cellular fatty acids were anteiso-C15:0, iso-C15:0 and anteiso-C17:0 and the DNA G + C content was 63.3 mol%. Phylogenetic analysis based on 16S rRNA gene sequence comparisons revealed that
strain JSM 078085T should be assigned to the genus Arthrobacter, being most closely related to the type strain of Arthrobacter rhombi (sequence similarity 97.1%), and the two strains formed a distinct lineage in the phylogenetic tree. The level of DNA–DNA
relatedness between strain JSM 078085T and the type strain of Arthrobacter rhombi was 10.6%. The combination of phylogenetic analysis, DNA–DNA relatedness, phenotypic characteristics and chemotaxonomic data
supported the view that strain JSM 078085T represents a novel species of the genus Arthrobacter, for which the name Arthrobacter halodurans sp. nov. is proposed. The type strain is JSM 078085T (=DSM 21081T=KCTC 19430T).
The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain JSM 078085T is EU583729. 相似文献
11.
Lei Wang Wen-Yan Liu Zhi-Jing Gu San-Feng Chen Su-Sheng Yang 《Journal of microbiology (Seoul, Korea)》2010,48(3):312-317
Three Gram reaction positive, rod-shaped, moderately motile halophilic bacterial strains, designated YD3-56T, YD16, and YH29, were isolated from the sediments of Manasi and Aiding salt lakes in the Xinjiang region of China, respectively.
The strains grew optimally at 30–37°C, pH 8–11, in the presence of 5–10% (w/v) NaCl. Phylogenetic analysis based on 16S rRNA
gene sequences revealed that the strains were closely related to members of the genus Oceanobacillus, exhibiting 99.1–99.2% similarity to O. kapialis KCTC 13177T, 99.2–99.3% to O. picturae KCTC 3821T, and 94.2–96% sequence similarity to other described Oceanobacillus species. SDS-PAGE of whole cell proteins preparations demonstrated that the strains exhibited high similarity to each other,
but distinguished from O. kapialis KCTC 13177T and O. picturae KCTC 3821T (75%). DNA-DNA hybridization revealed that the similarity between the representative strain YD3-56T and O. kapialis KCTC 13177T was 35.3%, and the similarity between YD3-56T and O. picturae KCTC 3821T was 22.3%. Chemotaxonomic analysis of the strains showed menaquinone-7 was the predominant respiratory quinine. Major cellular
fatty acids were anteiso-C15:0 and anteiso-C17:0. The polar lipid pattern for strain YD3-56T predominantly contained phosphatidylcholine, and trace to moderate amounts of phosphatidyl ethanolamine and hydroxy-phosphatidyl
ethanolamine. The diamino acid in murein was meso-diaminopimelic acid. The DNA G+C content of the strains was 39.7–40.1 mol%. On the basis of these results, the three strains
should be classified as a novel species of the genus Oceanobacillus, for which the name Oceanobacillus manasiensis sp. nov. has been proposed, with the type strain as YD3-56T (=CGMCC 1.9105T =NBRC 105903T). 相似文献
12.
Yi-Guang Chen Zhi-Xiong Liu Yu-Qin Zhang You-Xiang Zhang Shu-Kun Tang Entomack Borrathybay Wen-Jun Li Xiao-Long Cui 《Antonie van Leeuwenhoek》2009,96(1):99-107
A moderately halophilic, Gram-positive, catalase- and oxidase-positive, rod-shaped, aerobic bacterium, designated strain JSM
071068T, was isolated from a sea anemone (Anthopleura xanthogrammica) collected from the Naozhou Island on the Leizhou Bay in the South China Sea. Cells were motile by means of peritrichous
flagella and formed ellipsoidal endospores lying in subterminal swollen sporangia. Strain JSM 071068T was able to grow with 1–20% (w/v) total salts (optimum, 6–9%), at pH values of 6.0–10.0 (optimum, pH 7.5) and a temperature
range of 10–35°C (optimum, 25°C). meso-Diaminopimelic acid was present in the cell-wall peptidoglycan. The predominant menaquinone was MK-7 and the major cellular
fatty acids were anteiso-C15:0, anteiso-C17:0 and iso-C15:0. The genomic DNA G + C content was 42.8 mol%. Phylogenetic analysis based on 16S rRNA gene sequence comparisons revealed
that strain JSM 071068T belonged to the genus Halobacillus. The 16S rRNA gene sequence similarities between strain JSM 071068T and the type strains of the recognized Halobacillus species ranged from 97.9% (with Halobacillus alkaliphilus) to 95.3% (with Halobacillus kuroshimensis). The levels of DNA–DNA relatedness between the new isolate and the type strains of H. alkaliphilus, Halobacillus campisalis, Halobacillus halophilus and Halobacillus seohaensis were 25.6, 22.1, 10.8 and 13.2%, respectively. The combination of phylogenetic analysis, DNA–DNA relatedness, phenotypic
characteristics and chemotaxonomic data supported the view that strain JSM 071068T represents a new species of the genus Halobacillus, for which the name Halobacillus naozhouensis sp. nov. is proposed, with JSM 071068T (=DSM 21183T =KCTC 13234T) as the type strain.
The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain JSM 071068T is EU925615. 相似文献
13.
Cui CH Choi TE Yu H Jin F Lee ST Kim SC Im WT 《Journal of microbiology (Seoul, Korea)》2011,49(3):393-398
The Gram-negative, strictly aerobic, non-motile, non-spore-forming, rod shaped bacterial strain designated TR6-03T was isolated from compost, and its taxonomic position was investigated by using a polyphasic approach. Strain TR6-03T grew at 4–42°C and at pH 6.0–8.0 on R2A and nutrient agar without NaCl supplement. Strain TR6-03T had β-glucosidase activity, which was responsible for its ability to transform ginsenoside Re (one of the dominant active components
of ginseng) to Rg2. On the basis of 16S rRNA gene sequence similarity, strain TR6-03T was shown to belong to the family Sphingobacteriaceae and to be related to Mucilaginibacter lappiensis ANJLI2T (96.3% sequence similarity), M. dorajii FR-f4T (96.1%), and M. rigui WPCB133T (94.1%). The G+C content of the genomic DNA was 45.6%. The predominant respiratory quinone was MK-7 and the major fatty acids
were summed feature 3 (comprising C16:1 ω7c and/or iso-C15:0 20H), iso-C16:0 and iso-C17:0 3OH. DNA and chemotaxonomic data supported the affiliation of strain TR6-03T to the genus Mucilaginibacter. Strain TR6-03T could be differentiated genotypically and phenotypically from the recognized species of the genus Mucilaginibacter. The isolate therefore represents a novel species, for which the name Mucilaginibacter composti sp. nov. is proposed, with the type strain TR6-03T (=KACC 14956T = KCTC 12642T =LMG 23497T). 相似文献
14.
A Gram-negative, non-motile, catalase- and oxidase- positive, strictly aerobic, and short rod-shaped bacterium that was designated
strain KOPRI 25157T was isolated from coastal seawater sample in Antarctica. The temperature and pH ranges for growth on R2A agar were 10–20°C,
and 5.0–10.0, respectively. Phylogenetic analyses of the 16S rRNA gene sequence of strain KOPRI 25157T showed it to belong to the family Oxalobacteraceae of the class Betaproteobacteria, and it formed a distinct clade from other recognized members of the family. DNA G + C content was 65.9 mol%. Major ubiquinone
was Q-8. Predominant cellular fatty acids were C16:1
ω7c/15 iso 2OH (56.4%) and C16:1 (30.5%). Major polar lipids were phosphatidylglycerol, phosphatidylethanolamine, and unknown lipid. On the basis of these
data, it is proposed that strain KOPRI 25157T is the representative of a novel genus, for which the name Actimicrobium gen. nov. is proposed in the family Oxalobacteraceae. The type strain for Actimicrobium antarcticum sp. nov. is KOPRI 25157T (=JCM 16673T=KCTC 23040T). 相似文献
15.
Chen C Shi R Liu BB Zhang YJ Sun HZ Li CT Tang SK Zhang LL Li WJ 《Antonie van Leeuwenhoek》2011,100(3):365-373
Two moderately halophilic, Gram-negative, rod-shaped bacteria, designated YIM 93003T and YIM 94343T, were isolated from a salt lake in Xinjiang province, north-west China. The two strains YIM 93003T and YIM 94343T grew at 20–40°C, pH 6–9, 0.5–24% (w/v) NaCl and at 20–40°C, pH 6–9, 0.5–23% (w/v) NaCl, respectively. No growth occurred
in absence of NaCl. Phylogenetic analyses based on 16S rRNA gene sequences showed that strains YIM 93003T and YIM 94343T were phylogenetically affiliated to the genus Halomonas and exhibited sequence similarity of 97.5% and 97.4% to the type strain Halomonas anticariensis DSM 16096T, respectively. The strains possessed chemotaxonomic markers that were consistent with their classification in the genus Halomonas (Q-9 as predominant respiratory quinine; C18:1ω7c, C16:0 and C16:1 ω7c/iso-C15:02-OH as the major fatty acids). The DNA–DNA hybridization values for strains YIM 93003T and YIM 94343T, YIM 93003T and DSM 16096T, YIM 94343T and DSM 16096T were 38.1 ± 3.0, 18.3 ± 4.7, and 20.8 ± 4.6%, respectively. The G+C contents of the strains YIM 93003T and YIM 94343T were 63.4 and 64.0 mol%, respectively. Based on comparative analysis of physiological, biochemical and chemotaxonomic data,
including low DNA–DNA hybridization results, two novel species, Halomonas qijiaojingensis sp. nov., and Halomonas
flava sp. nov., are proposed. The type strains are YIM 93003T (=CCTCC AB 208133T =KCTC 22228T) and YIM 94343T (=CCTCC AB 2010382T =KCTC 23356T), respectively. 相似文献
16.
The gram-reaction-negative, motile, facultatively anaerobic, catalase-positive, oxidase-positive bacterial strain M3-4T was isolated from black sea sand and subjected to a taxonomic study. Cells of strain M3-4T have monotrichous flagella, grow optimally at 37°C and at pH 7–8 in the presence of 1–4% (w/v) NaCl and hydrolyze casein,
starch and l-tyrosine. According to phylogenetic analyses using 16S rRNA gene sequences, strain M3-4T belongs to the genus Photobacterium and is most closely related to Photobacterium rosenbergii LMG 22223T (97.4%) and P. gaetbulicola KCTC 22804T (96.6%). The DNA–DNA relatedness value between M3-4T and P. rosenbergii LMG 22223T was 21.5%. The DNA G+C mol% of strain M3-4T was 53.6. The major cellular fatty acid of strain M3-4T was a summed feature 3 consisting of C16:1 ω7c and/or iso-C15:0 2-OH (35.0%), followed by C16:0 (25.4%) and C18:1ω7c (16.8%). These data suggest that strain M3-4T represents a novel species in genus Photobacterium, for which the name P. atrarenae sp. nov. is proposed. The type strain is M3-4T (= KCTC 23265T = NCAIM B 02414T). 相似文献
17.
An aerobic, non-motile, catalase-positive, Gram-stain positive actinomycete designated YIM 63233T was isolated from the surface-sterilized leaves of Artemisia annua L. and characterized using a polyphasic taxonomic approach. Optimal growth occurred at 20–28°C, pH 6.0–7.0 and in the presence
of 0–3% (w/v) NaCl. 16S rRNA gene sequence-based phylogenetic analysis showed that strain YIM 63233T clustered with species of the genus Pseudonocardia, displaying ≥1.2% sequence divergence with recognized species of this genus (from 98.8 to 94.0%). Relatively low levels of
DNA–DNA relatedness were found between strain YIM 63233T and Pseudonocardia petroleophila IMSNU 22072T, which supported the classification of strain YIM 63233T within a novel species of the genus Pseudonocardia. The G + C content of genomic DNA was 72.0 mol%. Strain YIM 63233T possessed chemotaxonomic markers that were consistent with classification in the genus Pseudonocardia, i.e. the predominant fatty acids were iso-C16:0 (32.27%), C16:0 10-methyl (8.73%) and C17:1ω8c (8.30%), whilst the predominant
menaquinone was MK-8(H4). The diagnostic diamino acid of the cell-wall peptidoglycan was meso-diaminopimelic acid. The major cell wall sugars were glucose, arabinose, galactose, mannose and rhamnose. The results of
physiological and biochemical tests and DNA–DNA hybridization allowed the phenotypic and genotypic differentiation of strain
YIM 63233T from its closest phylogenetic neighbours. Therefore, the new isolate YIM 63233T represents a novel species of the genus Pseudonocardia, for which the name Pseudonocardia serianimatus sp. nov. is proposed. The type strain is YIM 63233T (=DSM 45302T = CCTCC AA 208079T). 相似文献
18.
Wan-Taek Im Qing-Mei Liu Jung-Eun Yang Min-Seok Kim Se-Young Kim Sung-Taik Lee Tae-Hoo Yi 《Journal of microbiology (Seoul, Korea)》2010,48(2):262-266
A taxonomic study was carried out on Gsoil 142T, a bacterial strain isolated from the soil collected in a ginseng field in Pocheon province, South Korea. Comparative 16S
rRNA gene sequence studies showed a clear affiliation of this bacterium to the Gammaproteobacteria, and it was most closely related to Hydrocarboniphaga effusa ATCC BAA 332T (94.4%, 16S rRNA gene sequence similarity), Nevskia ramosa DSM 11499T (94.1%) and Alkanibacter difficilis MN154.3T (92.0%). Strain Gsoil 142T was a Gram-negative, strictly aerobic, motile, and rod-shaped bacterium. The G+C content of the genomic DNA was 69.9% and
predominant ubiquinone was Q-8. Major fatty acids were summed feature 8 (C18:1
ω7c and/or ω6c, 36.3%), summed feature 3 (iso-C15:0 2-OH and/or C16:1
ω7c, 20.6%) and C16:0 (17.4%). The major polar lipids detected in strain Gsoil 142T were phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, and an unknown glycolipid. On the basis of polyphasic
evidence, it is proposed that strain Gsoil 142T should be placed in a novel genus and species, for which the name Panacagrimonas perspica gen. nov., sp. nov. is proposed. The type strain is Gsoil 142T (= KCTC 12982T = LMG 23239T). 相似文献
19.
Hana Kim Hyun-Myung Oh Seung-Jo Yang Jung-Sook Lee Jae-Sang Hong Jang-Cheon Cho 《Current microbiology》2009,58(5):511-515
A marine bacterium designated strain IMCC4074T was isolated from surface seawater collected off Incheon Port, the Yellow Sea, and subjected to a polyphasic taxonomy. The
strain was Gram-negative, chemoheterotrophic, slightly halophilic, strictly aerobic, and motile rods. Based on 16S rRNA gene
sequence comparisons, the strain was most closely related to Marinobacterium litorale KCTC 12756T (93.9%) and shared low 16S rRNA gene sequence similarities with members of the genus Marinobacterium (91.8–93.9%) and the genus Neptunomonas (93.4%) in the order Oceanospirillales. Phylogenetic analyses showed that this marine isolate formed an independent phyletic line within the genus Marinobacterium clade. The DNA G+C composition of the strain was 56.0 mol% and the predominant constituents of the cellular fatty acids were
C16:0 (28.0%), C16:1 ω7c and/or iso-C15:0 2-OH (19.3%), C18:1 ω7c (17.8%), and C17:1 cyclo (12.5%), which differentiated the strain from other Marinobacterium species. Based on the taxonomic data collected in this study, only a distant relationship could be found between strain IMCC4074T and other members of the genus Marinobacterium, thus the strain represents a novel species of the genus Marinobacterium, for which the name Marinobacterium marisflavi sp. nov. is proposed. The type strain of Marinobacterium marisflavi is IMCC4074T (= KCTC 12757T = LMG 23873T).
The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain IMCC4074T is EF468717.
An erratum to this article can be found at 相似文献
20.
Miao Q Qin S Bian GK Yuan B Xing K Zhang YJ Li Q Tang SK Li WJ Jiang JH 《Antonie van Leeuwenhoek》2011,100(3):333-339
A novel actinomycete, designated KLBMP 1221T, was isolated from the surface-sterilized seeds of an oil-seed plant Jatropha curcas L. collected from Sichuan Province, south-west China and was characterized taxonomically by using a polyphasic approach.
Phylogenetic analyses based on 16S rRNA gene sequence showed that this strain formed a distinct phyletic line within the radiation
of the genus Amycolatopsis. The 16S rRNA gene sequence similarity indicated that strain KLBMP 1221T was most closely related to Amycolatopsis eurytherma NT202T (98.9%), Amycolatopsis tucumanensis ABOT (98.8%), Amycolatopsis thermoflava N1165T (98.6%) and Amycolatopsis methanolica IMSNU 20055T (98.5%). Strain KLBMP 1221T had morphological and chemotaxonomic properties that were consistent with its classification in the genus Amycolatopsis. However, DNA–DNA relatedness data and phenotypic differences clearly distinguished the isolate from its closest relatives.
Based on the combined genotypic and phenotypic evidence, it is proposed that strain KLBMP 1221T be classified as representative of a novel species for which the name Amycolatopsis
endophytica sp. nov. is proposed. The type strain is KLBMP 1221T (= KCTC 19776T = CCTCC AA 2010003T). 相似文献